Restriction map for
pF1KB8955
(Circular) (Six-base) MAPSORT of: pF1KB8955.seq Check: 4552 from: 1 to: 4109
pF1KB8955 4109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 410 1147 410
Size: 737 3372
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 730 1934 2366 3113 3381 372
Size: 358 1204 432 747 268 1100
Fragments arranged by size:
1204 1100 747 432 358 268
AflIII A'CryG_T
Cuts at: 1461 2565 3542 1461
Size: 1104 977 2028
Fragments arranged by size:
2028 1104 977
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AlwNI CAG_nnn'CTG
Cuts at: 1414 2981 1414
Size: 1567 2542
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 1113 3462 3718 1113
Size: 2349 256 1504
Fragments arranged by size:
2349 1504 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 467 771 1129 467
Size: 304 358 3447
Fragments arranged by size:
3447 358 304
BamHI G'GATC_C
Cuts at: 1134 1134
Size: 4109
BanI G'GyrC_C
Cuts at: 1125 1539 1787 1822 1125
Size: 414 248 35 3412
Fragments arranged by size:
3412 414 248 35
BanII G_rGCy'C
Cuts at: 281 382 1123 2153 281
Size: 101 741 1030 2237
Fragments arranged by size:
2237 1030 741 101
BbeI G_GCGC'C
Cuts at: 1543 1791 1543
Size: 248 3861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 201 235 90
Size: 34 77 34 3964
Fragments arranged by size:
3964 77 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 1629 1629
Size: 4109
BglI GCCn_nnn'nGGC
Cuts at: 268 268
Size: 4109
BglII A'GATC_T
Cuts at: 719 1624 3661 719
Size: 905 2037 1167
Fragments arranged by size:
2037 1167 905
BlpI GC'TnA_GC
Cuts at: 1221 1221
Size: 4109
Bme1580I G_kGCm'C
Cuts at: 208 1734 1827 2883 208
Size: 1526 93 1056 1434
Fragments arranged by size:
1526 1434 1056 93
BmgBI CAC'GTC
Cuts at: 267 267
Size: 4109
BmrI ACTGGGnnnn_n'
Cuts at: 1321 1402 1737 3881 1321
Size: 81 335 2144 1549
Fragments arranged by size:
2144 1549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 166 769 1008 136
Size: 30 603 239 3237
Fragments arranged by size:
3237 603 239 30
Bpu10I CC'TnA_GC
Cuts at: 439 893 439
Size: 454 3655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2164 2656 2954 1285
Size: 879 492 298 2440
Fragments arranged by size:
2440 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3813 3813
Size: 4109
BsaAI yAC'GTr
Cuts at: 138 1462 138
Size: 1324 2785
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 226 1540 1788 2490 3986 226
Size: 1314 248 702 1496 349
Fragments arranged by size:
1496 1314 702 349 248
BsaWI w'CCGG_w
Cuts at: 983 1298 1819 2497 2771 2918 3329 983
Size: 315 521 678 274 147 411 1763
Fragments arranged by size:
1763 678 521 411 315 274 147
BseYI C'CCAG_C
Cuts at: 1072 1534 2292 2869 3365 1072
Size: 462 758 577 496 1816
Fragments arranged by size:
1816 758 577 496 462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 552 306
Size: 246 3863
BsiEI CG_ry'CG
Cuts at: 104 263 1651 1697 2905 104
Size: 159 1388 46 1208 1308
Fragments arranged by size:
1388 1308 1208 159 46
BsiHKAI G_wGCw'C
Cuts at: 371 382 1123 1901 2091 2883 371
Size: 11 741 778 190 792 1597
Fragments arranged by size:
1597 792 778 741 190 11
Bsp1286I G_dGCh'C
Cuts at: 208 281 371 382 1123 1734 1827 1901 2091
Size: 73 90 11 741 611 93 74 190
Cuts at: 2091 2153 2883 208
Size: 62 730 1434
Fragments arranged by size:
1434 741 730 611 190 93 90 74
73 62 11
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1159 1373 1675 2056 1159
Size: 214 302 381 3212
Fragments arranged by size:
3212 381 302 214
BsrBI CCG'CTC
Cuts at: 50 438 2401 2455 3939 4104 50
Size: 388 1963 54 1484 165 55
Fragments arranged by size:
1963 1484 388 165 55 54
BsrDI GCAATG_nn'
Cuts at: 482 926 2021 482
Size: 444 1095 2570
Fragments arranged by size:
2570 1095 444
BsrFI r'CCGG_y
Cuts at: 2107 2288 2497 2107
Size: 181 209 3719
Fragments arranged by size:
3719 209 181
BssSI C'ACGA_G
Cuts at: 2380 2738 2380
Size: 358 3751
BstAPI GCAn_nnn'nTGC
Cuts at: 1486 1486
Size: 4109
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstEII G'GTnAC_C
Cuts at: 848 848
Size: 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 114 312 813 825 114
Size: 198 501 12 3398
Fragments arranged by size:
3398 501 198 12
BstYI r'GATC_y
Cuts at: 1 719 794 1134 1601 1624 1959 2205 3217
Size: 718 75 340 467 23 335 246 1012
Cuts at: 3217 3303 3315 3661 1
Size: 86 12 346 449
Fragments arranged by size:
1012 718 467 449 346 340 335 246
86 75 23 12
BtgI C'CryG_G
Cuts at: 197 272 401 197
Size: 75 129 3905
Fragments arranged by size:
3905 129 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 141 2059 2240 2403 141
Size: 1918 181 163 1847
Fragments arranged by size:
1918 1847 181 163
BtsI GCAGTG_nn'
Cuts at: 688 1352 688
Size: 664 3445
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DrdI GACnn_nn'nnGTC
Cuts at: 1815 2673 1815
Size: 858 3251
EaeI y'GGCC_r
Cuts at: 260 269 398 422 815 929 1694 1868 2259
Size: 9 129 24 393 114 765 174 391
Cuts at: 2259 2286 3631 260
Size: 27 1345 738
Fragments arranged by size:
1345 765 738 393 391 174 129 114
27 24 9
EagI C'GGCC_G
Cuts at: 260 1694 260
Size: 1434 2675
EarI CTCTTCn'nnn_
Cuts at: 286 349 2132 2342 286
Size: 63 1783 210 2053
Fragments arranged by size:
2053 1783 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2317 2627 2773 3919 2317
Size: 310 146 1146 2507
Fragments arranged by size:
2507 1146 310 146
EcoICRI GAG'CTC
Cuts at: 380 1121 380
Size: 741 3368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 166 372 730 769 1008 1934 2366 3113
Size: 30 206 358 39 239 926 432 747
Cuts at: 3113 3381 136
Size: 268 864
Fragments arranged by size:
926 864 747 432 358 268 239 206
39 30
EcoO109I rG'GnC_Cy
Cuts at: 464 498 1248 464
Size: 34 750 3325
Fragments arranged by size:
3325 750 34
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 639 2128 639
Size: 1489 2620
FspI TGC'GCA
Cuts at: 1890 1890
Size: 4109
HaeII r_GCGC'y
Cuts at: 145 1543 1791 2813 3792 145
Size: 1398 248 1022 979 462
Fragments arranged by size:
1398 1022 979 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1951 1981 1983 2013 1951
Size: 30 2 30 4047
Fragments arranged by size:
4047 30 30 2
HincII GTy'rAC
Cuts at: 411 867 1148 411
Size: 456 281 3372
Fragments arranged by size:
3372 456 281
HpaI GTT'AAC
Cuts at: 867 867
Size: 4109
KasI G'GCGC_C
Cuts at: 1539 1787 1539
Size: 248 3861
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2964 2780
Size: 184 3925
MscI TGG'CCA
Cuts at: 424 817 931 1870 424
Size: 393 114 939 2663
Fragments arranged by size:
2663 939 393 114
MslI CAynn'nnrTG
Cuts at: 823 2098 2225 823
Size: 1275 127 2707
Fragments arranged by size:
2707 1275 127
MspA1I CmG'CkG
Cuts at: 199 789 1220 1534 1894 2907 3152 199
Size: 590 431 314 360 1013 245 1156
Fragments arranged by size:
1156 1013 590 431 360 314 245
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 1540 1788 1540
Size: 248 3861
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NspI r_CATG'y
Cuts at: 862 949 1162 1662 2569 862
Size: 87 213 500 907 2402
Fragments arranged by size:
2402 907 500 213 87
PasI CC'CwG_GG
Cuts at: 578 578
Size: 4109
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 4077
PpuMI rG'GwC_Cy
Cuts at: 464 498 464
Size: 34 4075
PsiI TTA'TAA
Cuts at: 697 697
Size: 4109
PspXI vC'TCGA_Gb
Cuts at: 771 771
Size: 4109
PstI C_TGCA'G
Cuts at: 224 739 1156 1841 224
Size: 515 417 685 2492
Fragments arranged by size:
2492 685 515 417
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 1534 1894 1534
Size: 360 3749
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 382 1123 382
Size: 741 3368
SacII CC_GC'GG
Cuts at: 200 200
Size: 4109
SalI G'TCGA_C
Cuts at: 409 1146 409
Size: 737 3372
SanDI GG'GwC_CC
Cuts at: 464 464
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 286 2132 2342 286
Size: 1846 210 2053
Fragments arranged by size:
2053 1846 210
SbfI CC_TGCA'GG
Cuts at: 739 1156 739
Size: 417 3692
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 220 735 1152 1837 2830 3021 33
Size: 187 515 417 685 993 191 1121
Fragments arranged by size:
1121 993 685 515 417 191 187
SfiI GGCCn_nnn'nGGCC
Cuts at: 268 268
Size: 4109
SfoI GGC'GCC
Cuts at: 1541 1789 1541
Size: 248 3861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 1131 1131
Size: 4109
SmlI C'TyrA_G
Cuts at: 771 1264 2179 2671 2933 771
Size: 493 915 492 262 1947
Fragments arranged by size:
1947 915 493 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 862 1162 1662 862
Size: 300 500 3309
Fragments arranged by size:
3309 500 300
StyI C'CwwG_G
Cuts at: 1243 1597 1243
Size: 354 3755
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 662 1692 1825 2284 2493 662
Size: 1030 133 459 209 2278
Fragments arranged by size:
2278 1030 459 209 133
TatI w'GTAC_w
Cuts at: 3624 3624
Size: 4109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4077
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 533 648 1604 240
Size: 293 115 956 2745
Fragments arranged by size:
2745 956 293 115
XhoI C'TCGA_G
Cuts at: 771 771
Size: 4109
XmaI C'CCGG_G
Cuts at: 1129 1129
Size: 4109
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PsiI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PspOMI PsrI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI