Restriction map for
pF1KB8953
(Circular) (Six-base) MAPSORT of: pF1KB8953.seq Check: 5566 from: 1 to: 4097
pF1KB8953 4097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 405 405
Size: 4097
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 1922 2354 3101 3369 696
Size: 1226 432 747 268 1424
Fragments arranged by size:
1424 1226 747 432 268
AflIII A'CryG_T
Cuts at: 128 1449 2553 3530 128
Size: 1321 1104 977 695
Fragments arranged by size:
1321 1104 977 695
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AhdI GACnn_n'nnGTC
Cuts at: 492 492
Size: 4097
AleI CACnn'nnGTG
Cuts at: 632 632
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 1402 2969 1402
Size: 1567 2530
ApaLI G'TGCA_C
Cuts at: 360 2867 360
Size: 2507 1590
ApoI r'AATT_y
Cuts at: 810 1101 3450 3706 810
Size: 291 2349 256 1201
Fragments arranged by size:
2349 1201 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 459 528 872 1117 459
Size: 69 344 245 3439
Fragments arranged by size:
3439 344 245 69
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 177 250 388 664 1113 1527 1775 1810 177
Size: 73 138 276 449 414 248 35 2464
Fragments arranged by size:
2464 449 414 276 248 138 73 35
BanII G_rGCy'C
Cuts at: 115 879 1111 2141 115
Size: 764 232 1030 2071
Fragments arranged by size:
2071 1030 764 232
BbeI G_GCGC'C
Cuts at: 254 1531 1779 254
Size: 1277 248 2572
Fragments arranged by size:
2572 1277 248
BbsI GAAGACnn'nnnn_
Cuts at: 516 516
Size: 4097
BbvCI CC'TCA_GC
Cuts at: 550 550
Size: 4097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 370 404 370
Size: 34 4063
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 517 517
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 169 1209 169
Size: 1040 3057
Bme1580I G_kGCm'C
Cuts at: 364 1722 1815 2871 364
Size: 1358 93 1056 1590
Fragments arranged by size:
1590 1358 1056 93
BmgBI CAC'GTC
Cuts at: 165 501 165
Size: 336 3761
BmrI ACTGGGnnnn_n'
Cuts at: 342 1309 1390 1725 3869 342
Size: 967 81 335 2144 570
Fragments arranged by size:
2144 967 570 335 81
BmtI G_CTAG'C
Cuts at: 788 788
Size: 4097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 388 777 142
Size: 246 389 3462
Fragments arranged by size:
3462 389 246
Bpu10I CC'TnA_GC
Cuts at: 550 550
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 500 3801 500
Size: 3301 796
BsaAI yAC'GTr
Cuts at: 129 1450 129
Size: 1321 2776
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 200 251 1528 1776 2478 3974 200
Size: 51 1277 248 702 1496 323
Fragments arranged by size:
1496 1277 702 323 248 51
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 115
Size: 30 4067
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 4097
BseYI C'CCAG_C
Cuts at: 409 468 1522 2280 2857 3353 409
Size: 59 1054 758 577 496 1153
Fragments arranged by size:
1153 1054 758 577 496 59
BsiEI CG_ry'CG
Cuts at: 104 308 322 341 482 521 1639 1685 2893
Size: 204 14 19 141 39 1118 46 1208
Cuts at: 2893 104
Size: 1308
Fragments arranged by size:
1308 1208 1118 204 141 46 39 19
14
BsiHKAI G_wGCw'C
Cuts at: 115 170 364 1111 1889 2079 2871 115
Size: 55 194 747 778 190 792 1341
Fragments arranged by size:
1341 792 778 747 194 190 55
BsiWI C'GTAC_G
Cuts at: 449 449
Size: 4097
BsmI GAATG_Cn'
Cuts at: 750 750
Size: 4097
BsmBI CGTCTCn'nnnn_
Cuts at: 687 687
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 115 170 364 879 1111 1722 1815 1889 2079
Size: 55 194 515 232 611 93 74 190
Cuts at: 2079 2141 2871 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 515 232 194 190 93
74 62 55
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 405 1147 1361 1663 2044 405
Size: 742 214 302 381 2458
Fragments arranged by size:
2458 742 381 302 214
BsrBI CCG'CTC
Cuts at: 50 194 2389 2443 3927 4092 50
Size: 144 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 444 2095 2276 2485 444
Size: 1651 181 209 2056
Fragments arranged by size:
2056 1651 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 1002 1002
Size: 4097
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 209 401 209
Size: 192 3905
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 364 1340 364
Size: 976 3121
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 922 1803 2661 922
Size: 881 858 2358
Fragments arranged by size:
2358 881 858
EaeI y'GGCC_r
Cuts at: 275 305 319 338 479 518 555 624 986
Size: 30 14 19 141 39 37 69 362
Cuts at: 986 1682 1856 2247 2274 3619 275
Size: 696 174 391 27 1345 753
Fragments arranged by size:
1345 753 696 391 362 174 141 69
39 37 30 27 19 14
EagI C'GGCC_G
Cuts at: 305 319 338 479 518 1682 305
Size: 14 19 141 39 1164 2720
Fragments arranged by size:
2720 1164 141 39 19 14
EarI CTCTTCn'nnn_
Cuts at: 733 2120 2330 733
Size: 1387 210 2500
Fragments arranged by size:
2500 1387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 1109 113
Size: 996 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 388 696 777 1922 2354 3101 3369 142
Size: 246 308 81 1145 432 747 268 870
Fragments arranged by size:
1145 870 747 432 308 268 246 81
EcoO109I rG'GnC_Cy
Cuts at: 374 1236 374
Size: 862 3235
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 254 1531 1779 2801 3780 254
Size: 1277 248 1022 979 571
Fragments arranged by size:
1277 1022 979 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 478 510 1939 1969 1971 2001 115
Size: 32 331 32 1429 30 2 30 2211
Fragments arranged by size:
2211 1429 331 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 250 1527 1775 250
Size: 1277 248 2572
Fragments arranged by size:
2572 1277 248
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 1038 2768 2952 221
Size: 817 1730 184 1366
Fragments arranged by size:
1730 1366 817 184
MscI TGG'CCA
Cuts at: 988 1858 988
Size: 870 3227
MslI CAynn'nnrTG
Cuts at: 632 911 1000 2086 2213 632
Size: 279 89 1086 127 2516
Fragments arranged by size:
2516 1086 279 127 89
MspA1I CmG'CkG
Cuts at: 146 263 403 409 554 1208 1522 1882 2895
Size: 117 140 6 145 654 314 360 1013
Cuts at: 2895 3140 146
Size: 245 1103
Fragments arranged by size:
1103 1013 654 360 314 245 145 140
117 6
NaeI GCC'GGC
Cuts at: 446 2278 446
Size: 1832 2265
NarI GG'CG_CC
Cuts at: 251 1528 1776 251
Size: 1277 248 2572
Fragments arranged by size:
2572 1277 248
NgoMIV G'CCGG_C
Cuts at: 444 2276 444
Size: 1832 2265
NheI G'CTAG_C
Cuts at: 784 784
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PspXI vC'TCGA_Gb
Cuts at: 872 872
Size: 4097
PstI C_TGCA'G
Cuts at: 221 1144 1829 221
Size: 923 685 2489
Fragments arranged by size:
2489 923 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 263 409 1522 1882 263
Size: 146 1113 360 2478
Fragments arranged by size:
2478 1113 360 146
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 115 1111 115
Size: 996 3101
SacII CC_GC'GG
Cuts at: 404 404
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 865 3614 865
Size: 2749 1348
SexAI A'CCwGG_T
Cuts at: 384 384
Size: 4097
SfcI C'TryA_G
Cuts at: 33 217 1140 1825 2818 3009 33
Size: 184 923 685 993 191 1121
Fragments arranged by size:
1121 993 923 685 191 184
SfoI GGC'GCC
Cuts at: 252 1529 1777 252
Size: 1277 248 2572
Fragments arranged by size:
2572 1277 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 461 1119 461
Size: 658 3439
SmlI C'TyrA_G
Cuts at: 872 1252 2167 2659 2921 872
Size: 380 915 492 262 2048
Fragments arranged by size:
2048 915 492 380 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 1231 1585 1231
Size: 354 3743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1680 1813 2272 2481 195
Size: 1485 133 459 209 1811
Fragments arranged by size:
1811 1485 459 209 133
TatI w'GTAC_w
Cuts at: 863 3612 863
Size: 2749 1348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 981 1013 3614 3646 981
Size: 32 2601 32 1432
Fragments arranged by size:
2601 1432 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XhoI C'TCGA_G
Cuts at: 872 872
Size: 4097
XmaI C'CCGG_G
Cuts at: 459 1117 459
Size: 658 3439
XmnI GAAnn'nnTTC
Cuts at: 980 980
Size: 4097
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PciI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BfrBI BsgI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI