Restriction map for
pF1KB8951
(Circular) (Six-base) MAPSORT of: pF1KB8951.seq Check: 3585 from: 1 to: 4091
pF1KB8951 4091 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 352 352
Size: 4091
AccI GT'mk_AC
Cuts at: 650 1129 650
Size: 479 3612
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1916 2348 3095 3363 817
Size: 1099 432 747 268 1545
Fragments arranged by size:
1545 1099 747 432 268
AflII C'TTAA_G
Cuts at: 1006 1006
Size: 4091
AflIII A'CryG_T
Cuts at: 1443 2547 3524 1443
Size: 1104 977 2010
Fragments arranged by size:
2010 1104 977
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 225 1396 2963 225
Size: 1171 1567 1353
Fragments arranged by size:
1567 1353 1171
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 1095 3444 3700 1095
Size: 2349 256 1486
Fragments arranged by size:
2349 1486 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 181 1111 181
Size: 930 3161
BamHI G'GATC_C
Cuts at: 503 1116 503
Size: 613 3478
BanI G'GyrC_C
Cuts at: 283 1107 1521 1769 1804 283
Size: 824 414 248 35 2570
Fragments arranged by size:
2570 824 414 248 35
BanII G_rGCy'C
Cuts at: 388 602 1105 2135 388
Size: 214 503 1030 2344
Fragments arranged by size:
2344 1030 503 214
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BbsI GAAGACnn'nnnn_
Cuts at: 708 708
Size: 4091
BbvCI CC'TCA_GC
Cuts at: 841 841
Size: 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4057
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 605 1611 605
Size: 1006 3085
BglI GCCn_nnn'nGGC
Cuts at: 403 403
Size: 4091
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 182 288 1716 1809 2865 182
Size: 106 1428 93 1056 1408
Fragments arranged by size:
1428 1408 1056 106 93
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4091
Bpu10I CC'TnA_GC
Cuts at: 841 841
Size: 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 177 3795 177
Size: 3618 473
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 1522 1770 2472 3968 1522
Size: 248 702 1496 1645
Fragments arranged by size:
1645 1496 702 248
BsaWI w'CCGG_w
Cuts at: 363 446 1280 1801 2479 2753 2900 3311 363
Size: 83 834 521 678 274 147 411 1143
Fragments arranged by size:
1143 834 678 521 411 274 147 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 750 679
Size: 71 4020
BseYI C'CCAG_C
Cuts at: 904 1516 2274 2851 3347 904
Size: 612 758 577 496 1648
Fragments arranged by size:
1648 758 612 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 1025
Size: 4091
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 161 388 1105 1883 2073 2865 161
Size: 227 717 778 190 792 1387
Fragments arranged by size:
1387 792 778 717 227 190
Bsp1286I G_dGCh'C
Cuts at: 161 182 288 388 602 1105 1716 1809 1883
Size: 21 106 100 214 503 611 93 74
Cuts at: 1883 2073 2135 2865 161
Size: 190 62 730 1387
Fragments arranged by size:
1387 730 611 503 214 190 106 100
93 74 62 21
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 352 1141 1355 1657 2038 352
Size: 789 214 302 381 2405
Fragments arranged by size:
2405 789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 567 2383 2437 3921 4086 50
Size: 517 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 517 165 55 54
BsrDI GCAATG_nn'
Cuts at: 920 2003 920
Size: 1083 3008
BsrFI r'CCGG_y
Cuts at: 493 2089 2270 2479 493
Size: 1596 181 209 2105
Fragments arranged by size:
2105 1596 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstEII G'GTnAC_C
Cuts at: 462 462
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 813 813
Size: 4091
BstYI r'GATC_y
Cuts at: 1 437 503 1059 1116 1583 1606 1941 2187
Size: 436 66 556 57 467 23 335 246
Cuts at: 2187 3199 3285 3297 3643 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 556 467 449 436 346 335 246
86 66 57 23 12
BtgI C'CryG_G
Cuts at: 278 278
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 147 2041 2222 2385 147
Size: 1894 181 163 1853
Fragments arranged by size:
1894 1853 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 395 430 395
Size: 35 4056
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 275 485 950 1676 1850 2241 2268 3613 275
Size: 210 465 726 174 391 27 1345 753
Fragments arranged by size:
1345 753 726 465 391 210 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 832 886 1064 2114 2324 832
Size: 54 178 1050 210 2599
Fragments arranged by size:
2599 1050 210 178 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 386 1103 386
Size: 717 3374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 817 1916 2348 3095 3363 730
Size: 87 1099 432 747 268 1458
Fragments arranged by size:
1458 1099 747 432 268 87
EcoO109I rG'GnC_Cy
Cuts at: 1230 1230
Size: 4091
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 226 1525 1773 2795 3774 226
Size: 1299 248 1022 979 543
Fragments arranged by size:
1299 1022 979 543 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1933 1963 1965 1995 1933
Size: 30 2 30 4029
Fragments arranged by size:
4029 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MfeI C'AATT_G
Cuts at: 407 407
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 487 1852 487
Size: 1365 2726
MslI CAynn'nnrTG
Cuts at: 154 634 2080 2207 154
Size: 480 1446 127 2038
Fragments arranged by size:
2038 1446 480 127
MspA1I CmG'CkG
Cuts at: 845 1031 1202 1516 1876 2889 3134 845
Size: 186 171 314 360 1013 245 1802
Fragments arranged by size:
1802 1013 360 314 245 186 171
NaeI GCC'GGC
Cuts at: 495 2272 495
Size: 1777 2314
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NgoMIV G'CCGG_C
Cuts at: 493 2270 493
Size: 1777 2314
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PstI C_TGCA'G
Cuts at: 1138 1823 1138
Size: 685 3406
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 845 1516 1876 845
Size: 671 360 3060
Fragments arranged by size:
3060 671 360
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 388 1105 388
Size: 717 3374
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 781 3608 781
Size: 2827 1264
SfcI C'TryA_G
Cuts at: 33 1134 1819 2812 3003 33
Size: 1101 685 993 191 1121
Fragments arranged by size:
1121 1101 993 685 191
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 183 1113 183
Size: 930 3161
SmlI C'TyrA_G
Cuts at: 1006 1246 2161 2653 2915 1006
Size: 240 915 492 262 2182
Fragments arranged by size:
2182 915 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StuI AGG'CCT
Cuts at: 1077 1077
Size: 4091
StyI C'CwwG_G
Cuts at: 1225 1579 1225
Size: 354 3737
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 394 660 1674 1807 2266 2475 271
Size: 123 266 1014 133 459 209 1887
Fragments arranged by size:
1887 1014 459 266 209 133 123
TatI w'GTAC_w
Cuts at: 641 779 3606 641
Size: 138 2827 1126
Fragments arranged by size:
2827 1126 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 588 1586 588
Size: 998 3093
XmaI C'CCGG_G
Cuts at: 181 1111 181
Size: 930 3161
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI