Restriction map for
pF1KB8947
(Circular) (Six-base) MAPSORT of: pF1KB8947.seq Check: 2748 from: 1 to: 4055
pF1KB8947 4055 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1880 2312 3059 3327 1880
Size: 432 747 268 2608
Fragments arranged by size:
2608 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 909 1360 2927 909
Size: 451 1567 2037
Fragments arranged by size:
2037 1567 451
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 1059 3408 3664 1059
Size: 2349 256 1450
Fragments arranged by size:
2349 1450 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 919 1071 1485 1733 1768 919
Size: 152 414 248 35 3206
Fragments arranged by size:
3206 414 248 152 35
BanII G_rGCy'C
Cuts at: 514 557 1069 2099 514
Size: 43 512 1030 2470
Fragments arranged by size:
2470 1030 512 43
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbsI GAAGACnn'nnnn_
Cuts at: 396 1029 396
Size: 633 3422
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 802 914 802
Size: 112 3943
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 619 1167 619
Size: 548 3507
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmgBI CAC'GTC
Cuts at: 486 486
Size: 4055
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 430 481 964 985 304
Size: 126 51 483 21 3374
Fragments arranged by size:
3374 483 126 51 21
Bpu10I CC'TnA_GC
Cuts at: 331 577 331
Size: 246 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1027 1231 2110 2602 2900 973
Size: 54 204 879 492 298 2128
Fragments arranged by size:
2128 879 492 298 204 54
BsaI GGTCTCn'nnnn_
Cuts at: 457 3759 457
Size: 3302 753
BsaAI yAC'GTr
Cuts at: 281 549 1408 281
Size: 268 859 2928
Fragments arranged by size:
2928 859 268
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 889 1486 1734 2436 3932 889
Size: 597 248 702 1496 1012
Fragments arranged by size:
1496 1012 702 597 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 251 281 430 460 251
Size: 30 149 30 3846
Fragments arranged by size:
3846 149 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 275 697 982 275
Size: 422 285 3348
Fragments arranged by size:
3348 422 285
BseYI C'CCAG_C
Cuts at: 384 571 1008 1480 2238 2815 3311 384
Size: 187 437 472 758 577 496 1128
Fragments arranged by size:
1128 758 577 496 472 437 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2829 1069
Size: 778 190 792 2295
Fragments arranged by size:
2295 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 514 557 1069 1680 1773 1847 2037 2099 2829
Size: 43 512 611 93 74 190 62 730
Cuts at: 2829 514
Size: 1740
Fragments arranged by size:
1740 730 611 512 190 93 74 62
43
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 395 3231 395
Size: 2836 1219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssHII G'CGCG_C
Cuts at: 527 597 527
Size: 70 3985
BssSI C'ACGA_G
Cuts at: 727 2326 2684 727
Size: 1599 358 2098
Fragments arranged by size:
2098 1599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 807 807
Size: 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 963 963
Size: 4055
BstYI r'GATC_y
Cuts at: 1 233 947 1080 1547 1570 1905 2151 3163
Size: 232 714 133 467 23 335 246 1012
Cuts at: 3163 3249 3261 3607 1
Size: 86 12 346 449
Fragments arranged by size:
1012 714 467 449 346 335 246 232
133 86 23 12
BtgI C'CryG_G
Cuts at: 107 558 564 743 107
Size: 451 6 179 3419
Fragments arranged by size:
3419 451 179 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 205 2005 2186 2349 205
Size: 1800 181 163 1911
Fragments arranged by size:
1911 1800 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 125 275 339 365 561 719 740 794 953
Size: 150 64 26 196 158 21 54 159
Cuts at: 953 1025 1640 1814 2205 2232 3577 125
Size: 72 615 174 391 27 1345 603
Fragments arranged by size:
1345 615 603 391 196 174 159 158
150 72 64 54 27 26 21
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 934 967 1003 2078 2288 934
Size: 33 36 1075 210 2701
Fragments arranged by size:
2701 1075 210 36 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 430 481 964 985 1880 2312 3059 3327
Size: 126 51 483 21 895 432 747 268
Cuts at: 3327 304
Size: 1032
Fragments arranged by size:
1032 895 747 483 432 268 126 51
21
EcoNI CCTnn'n_nnAGG
Cuts at: 679 955 679
Size: 276 3779
EcoO109I rG'GnC_Cy
Cuts at: 327 588 660 906 993 1194 327
Size: 261 72 246 87 201 3188
Fragments arranged by size:
3188 261 246 201 87 72
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 1014 2074 465
Size: 549 1060 2446
Fragments arranged by size:
2446 1060 549
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 821 866 1489 1737 2759 3738 821
Size: 45 623 248 1022 979 1138
Fragments arranged by size:
1138 1022 979 623 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 430 462 966 998 1897 1927 1929
Size: 32 173 32 504 32 899 30 2
Cuts at: 1929 1959 225
Size: 30 2321
Fragments arranged by size:
2321 899 504 173 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 127 277 955 1816 127
Size: 150 678 861 2366
Fragments arranged by size:
2366 861 678 150
MslI CAynn'nnrTG
Cuts at: 760 2044 2171 760
Size: 1284 127 2644
Fragments arranged by size:
2644 1284 127
MspA1I CmG'CkG
Cuts at: 304 363 417 1166 1480 1840 2853 3098 304
Size: 59 54 749 314 360 1013 245 1261
Fragments arranged by size:
1261 1013 749 360 314 245 59 54
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PasI CC'CwG_GG
Cuts at: 669 750 831 867 669
Size: 81 81 36 3857
Fragments arranged by size:
3857 81 81 36
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PfoI T'CCnGG_A
Cuts at: 407 941 407
Size: 534 3521
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PmlI CAC'GTG
Cuts at: 281 281
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PpuMI rG'GwC_Cy
Cuts at: 327 993 327
Size: 666 3389
PstI C_TGCA'G
Cuts at: 680 905 1102 1787 680
Size: 225 197 685 2948
Fragments arranged by size:
2948 685 225 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 417 1480 1840 417
Size: 1063 360 2632
Fragments arranged by size:
2632 1063 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 680 1102 680
Size: 422 3633
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 676 901 1098 1783 2776 2967 33
Size: 643 225 197 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 225 197 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 988 1042 1210 2125 2617 2879 988
Size: 54 168 915 492 262 2164
Fragments arranged by size:
2164 915 492 262 168 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 107 956 1189 1543 107
Size: 849 233 354 2619
Fragments arranged by size:
2619 849 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 639 1638 1771 2230 2439 639
Size: 999 133 459 209 2255
Fragments arranged by size:
2255 999 459 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 251 283 470 502 3572 3604 251
Size: 32 187 32 3070 32 702
Fragments arranged by size:
3070 702 187 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 828 1550 381
Size: 447 722 2886
Fragments arranged by size:
2886 722 447
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI