Restriction map for
pF1KB8946
(Circular) (Six-base) MAPSORT of: pF1KB8946.seq Check: 3170 from: 1 to: 4052
pF1KB8946 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 449 899 1090 449
Size: 450 191 3411
Fragments arranged by size:
3411 450 191
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 876 1877 2309 3056 3324 276
Size: 600 1001 432 747 268 1004
Fragments arranged by size:
1004 1001 747 600 432 268
AfeI AGC'GCT
Cuts at: 841 841
Size: 4052
AflIII A'CryG_T
Cuts at: 143 1404 2508 3485 143
Size: 1261 1104 977 710
Fragments arranged by size:
1261 1104 977 710
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 868 900 868
Size: 32 4020
AlwNI CAG_nnn'CTG
Cuts at: 383 1357 2924 383
Size: 974 1567 1511
Fragments arranged by size:
1567 1511 974
ApaI G_GGCC'C
Cuts at: 614 614
Size: 4052
ApaLI G'TGCA_C
Cuts at: 373 2822 373
Size: 2449 1603
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AscI GG'CGCG_CC
Cuts at: 662 662
Size: 4052
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 174 586 754 772 1072 174
Size: 412 168 18 300 3154
Fragments arranged by size:
3154 412 300 168 18
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 326 393 553 749 1068 1482 1730 1765 326
Size: 67 160 196 319 414 248 35 2613
Fragments arranged by size:
2613 414 319 248 196 160 67 35
BanII G_rGCy'C
Cuts at: 314 371 439 614 1066 2096 314
Size: 57 68 175 452 1030 2270
Fragments arranged by size:
2270 1030 452 175 68 57
BbeI G_GCGC'C
Cuts at: 397 753 1486 1734 397
Size: 356 733 248 2715
Fragments arranged by size:
2715 733 356 248
BbsI GAAGACnn'nnnn_
Cuts at: 215 773 215
Size: 558 3494
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 377 614 1677 1770 2826 377
Size: 237 1063 93 1056 1603
Fragments arranged by size:
1603 1063 1056 237 93
BmgBI CAC'GTC
Cuts at: 423 423
Size: 4052
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 798 174
Size: 624 3428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1004 3756 1004
Size: 2752 1300
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 394 509 750 1483 1731 2433 3929 394
Size: 115 241 733 248 702 1496 517
Fragments arranged by size:
1496 733 702 517 248 241 115
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 340 370 556 586 232
Size: 30 78 30 186 30 3698
Fragments arranged by size:
3698 186 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 240 379 564 745 1033 126
Size: 114 139 185 181 288 3145
Fragments arranged by size:
3145 288 185 181 139 114
BseYI C'CCAG_C
Cuts at: 397 1477 2235 2812 3308 397
Size: 1080 758 577 496 1141
Fragments arranged by size:
1141 1080 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 407 1594 1640 2848 104
Size: 303 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 303 46
BsiHKAI G_wGCw'C
Cuts at: 371 377 439 1066 1844 2034 2826 371
Size: 6 62 627 778 190 792 1597
Fragments arranged by size:
1597 792 778 627 190 62 6
BsmBI CGTCTCn'nnnn_
Cuts at: 177 706 177
Size: 529 3523
Bsp1286I G_dGCh'C
Cuts at: 314 371 377 439 614 1066 1677 1770 1844
Size: 57 6 62 175 452 611 93 74
Cuts at: 1844 2034 2096 2826 314
Size: 190 62 730 1540
Fragments arranged by size:
1540 730 611 452 190 175 93 74
62 62 57 6
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 1102 1316 1618 1999 266
Size: 836 214 302 381 2319
Fragments arranged by size:
2319 836 381 302 214
BsrBI CCG'CTC
Cuts at: 50 741 2344 2398 3882 4047 50
Size: 691 1603 54 1484 165 55
Fragments arranged by size:
1603 1484 691 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 406 666 805 2050 2231 2440 406
Size: 260 139 1245 181 209 2018
Fragments arranged by size:
2018 1245 260 209 181 139
BssHII G'CGCG_C
Cuts at: 662 662
Size: 4052
BssSI C'ACGA_G
Cuts at: 370 2323 2681 370
Size: 1953 358 1741
Fragments arranged by size:
1953 1741 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstYI r'GATC_y
Cuts at: 1 863 1077 1544 1567 1902 2148 3160 3246
Size: 862 214 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 862 467 449 346 335 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 107 333 359 494 623 635 107
Size: 226 26 135 129 12 3524
Fragments arranged by size:
3524 226 135 129 26 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 348 1295 348
Size: 947 3105
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 404 428 440 620 786 819 1637 1811 2202
Size: 24 12 180 166 33 818 174 391
Cuts at: 2202 2229 3574 404
Size: 27 1345 882
Fragments arranged by size:
1345 882 818 391 180 174 166 33
27 24 12
EagI C'GGCC_G
Cuts at: 404 1637 404
Size: 1233 2819
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 369 437 1064 369
Size: 68 627 3357
Fragments arranged by size:
3357 627 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 276 798 876 1877 2309 3056 3324 174
Size: 102 522 78 1001 432 747 268 902
Fragments arranged by size:
1001 902 747 522 432 268 102 78
EcoNI CCTnn'n_nnAGG
Cuts at: 262 517 262
Size: 255 3797
EcoO109I rG'GnC_Cy
Cuts at: 610 611 1191 610
Size: 1 580 3471
Fragments arranged by size:
3471 580 1
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 179 2071 179
Size: 1892 2160
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 397 602 753 843 921 1486 1734 2756 3735
Size: 205 151 90 78 565 248 1022 979
Cuts at: 3735 397
Size: 714
Fragments arranged by size:
1022 979 714 565 248 205 151 90
78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 393 749 1482 1730 393
Size: 356 733 248 2715
Fragments arranged by size:
2715 733 356 248
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 294 602 2723 2907 278
Size: 16 308 2121 184 1423
Fragments arranged by size:
2121 1423 308 184 16
MscI TGG'CCA
Cuts at: 430 821 1813 430
Size: 391 992 2669
Fragments arranged by size:
2669 992 391
MslI CAynn'nnrTG
Cuts at: 2041 2168 2041
Size: 127 3925
MspA1I CmG'CkG
Cuts at: 335 361 637 689 1163 1477 1837 2850 3095
Size: 26 276 52 474 314 360 1013 245
Cuts at: 3095 335
Size: 1292
Fragments arranged by size:
1292 1013 474 360 314 276 245 52
26
NaeI GCC'GGC
Cuts at: 408 668 807 2233 408
Size: 260 139 1426 2227
Fragments arranged by size:
2227 1426 260 139
NarI GG'CG_CC
Cuts at: 394 750 1483 1731 394
Size: 356 733 248 2715
Fragments arranged by size:
2715 733 356 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 406 666 805 2231 406
Size: 260 139 1426 2227
Fragments arranged by size:
2227 1426 260 139
NspI r_CATG'y
Cuts at: 147 939 1105 1605 2512 147
Size: 792 166 500 907 1687
Fragments arranged by size:
1687 907 792 500 166
PciI A'CATG_T
Cuts at: 143 2508 143
Size: 2365 1687
PflMI CCAn_nnn'nTGG
Cuts at: 323 323
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PspOMI G'GGCC_C
Cuts at: 610 610
Size: 4052
PstI C_TGCA'G
Cuts at: 392 1099 1784 392
Size: 707 685 2660
Fragments arranged by size:
2660 707 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 371 439 1066 371
Size: 68 627 3357
Fragments arranged by size:
3357 627 68
SacII CC_GC'GG
Cuts at: 336 362 638 336
Size: 26 276 3750
Fragments arranged by size:
3750 276 26
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 392 1099 392
Size: 707 3345
ScaI AGT'ACT
Cuts at: 1024 3569 1024
Size: 2545 1507
SfcI C'TryA_G
Cuts at: 33 388 486 1095 1780 2773 2964 33
Size: 355 98 609 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 355 191 98
SfoI GGC'GCC
Cuts at: 395 751 1484 1732 395
Size: 356 733 248 2715
Fragments arranged by size:
2715 733 356 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 588 1074 588
Size: 486 3566
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 939 1105 1605 939
Size: 166 500 3386
Fragments arranged by size:
3386 500 166
StyI C'CwwG_G
Cuts at: 107 1186 1540 107
Size: 1079 354 2619
Fragments arranged by size:
2619 1079 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 160 1022 3567 160
Size: 862 2545 645
Fragments arranged by size:
2545 862 645
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 3569 3601 340
Size: 32 3197 32 791
Fragments arranged by size:
3197 791 32 32
Tth111I GACn'n_nGTC
Cuts at: 219 1849 219
Size: 1630 2422
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 586 1072 586
Size: 486 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI