Restriction map for
pF1KB8945
(Circular) (Six-base) MAPSORT of: pF1KB8945.seq Check: 179 from: 1 to: 4025
pF1KB8945 4025 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 320 831 320
Size: 511 3514
AccI GT'mk_AC
Cuts at: 128 1063 128
Size: 935 3090
Acc65I G'GTAC_C
Cuts at: 702 1041 702
Size: 339 3686
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1850 2282 3029 3297 1850
Size: 432 747 268 2578
Fragments arranged by size:
2578 747 432 268
AflII C'TTAA_G
Cuts at: 421 421
Size: 4025
AflIII A'CryG_T
Cuts at: 779 1377 2481 3458 779
Size: 598 1104 977 1346
Fragments arranged by size:
1346 1104 977 598
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AhdI GACnn_n'nnGTC
Cuts at: 975 975
Size: 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 639 639
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 1330 2897 1330
Size: 1567 2458
ApaLI G'TGCA_C
Cuts at: 943 2795 943
Size: 1852 2173
ApoI r'AATT_y
Cuts at: 766 1029 3378 3634 766
Size: 263 2349 256 1157
Fragments arranged by size:
2349 1157 263 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 187 323 1045 187
Size: 136 722 3167
Fragments arranged by size:
3167 722 136
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 337 599 702 1041 1455 1703 1738 337
Size: 262 103 339 414 248 35 2624
Fragments arranged by size:
2624 414 339 262 248 103 35
BanII G_rGCy'C
Cuts at: 1039 2069 1039
Size: 1030 2995
BbeI G_GCGC'C
Cuts at: 341 1459 1707 341
Size: 1118 248 2659
Fragments arranged by size:
2659 1118 248
BbvCI CC'TCA_GC
Cuts at: 987 987
Size: 4025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 338 912 946 304
Size: 34 574 34 3383
Fragments arranged by size:
3383 574 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglI GCCn_nnn'nGGC
Cuts at: 406 553 589 406
Size: 147 36 3842
Fragments arranged by size:
3842 147 36
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 292 411 602 898 947 1650 1743 2799 292
Size: 119 191 296 49 703 93 1056 1518
Fragments arranged by size:
1518 1056 703 296 191 119 93 49
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 778 772
Size: 6 4019
Bpu10I CC'TnA_GC
Cuts at: 987 987
Size: 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 808 1539 3576 6
Size: 802 731 2037 455
Fragments arranged by size:
2037 802 731 455
BsaHI Gr'CG_yC
Cuts at: 338 692 1456 1704 2406 3902 338
Size: 354 764 248 702 1496 461
Fragments arranged by size:
1496 764 702 461 354 248
BsaWI w'CCGG_w
Cuts at: 797 1214 1735 2413 2687 2834 3245 797
Size: 417 521 678 274 147 411 1577
Fragments arranged by size:
1577 678 521 417 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 940 970 301
Size: 30 609 30 3356
Fragments arranged by size:
3356 609 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 309 309
Size: 4025
BseYI C'CCAG_C
Cuts at: 530 1450 2208 2785 3281 530
Size: 920 758 577 496 1274
Fragments arranged by size:
1274 920 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 609 298
Size: 311 3714
BsiEI CG_ry'CG
Cuts at: 104 548 584 1567 1613 2821 104
Size: 444 36 983 46 1208 1308
Fragments arranged by size:
1308 1208 983 444 46 36
BsiHKAI G_wGCw'C
Cuts at: 947 1039 1817 2007 2799 947
Size: 92 778 190 792 2173
Fragments arranged by size:
2173 792 778 190 92
BsmBI CGTCTCn'nnnn_
Cuts at: 266 266
Size: 4025
Bsp1286I G_dGCh'C
Cuts at: 292 411 602 898 947 1039 1650 1743 1817
Size: 119 191 296 49 92 611 93 74
Cuts at: 1817 2007 2069 2799 292
Size: 190 62 730 1518
Fragments arranged by size:
1518 730 611 296 191 190 119 93
92 74 62 49
BspEI T'CCGG_A
Cuts at: 797 3245 797
Size: 2448 1577
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 831 1075 1289 1591 1972 320
Size: 511 244 214 302 381 2373
Fragments arranged by size:
2373 511 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 272 2317 2371 3855 4020 50
Size: 222 2045 54 1484 165 55
Fragments arranged by size:
2045 1484 222 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 340 981 2023 2204 2413 340
Size: 641 1042 181 209 1952
Fragments arranged by size:
1952 1042 641 209 181
BssHII G'CGCG_C
Cuts at: 952 952
Size: 4025
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstEII G'GTnAC_C
Cuts at: 366 366
Size: 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 538 538
Size: 4025
BstYI r'GATC_y
Cuts at: 1 1050 1517 1540 1875 2121 3133 3219 3231
Size: 1049 467 23 335 246 1012 86 12
Cuts at: 3231 3577 1
Size: 346 449
Fragments arranged by size:
1049 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 466 548 584 655 677 466
Size: 82 36 71 22 3814
Fragments arranged by size:
3814 82 71 36 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 390 409 666 1268 390
Size: 19 257 602 3147
Fragments arranged by size:
3147 602 257 19
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 545 581 1610 1784 2175 2202 3547 545
Size: 36 1029 174 391 27 1345 1023
Fragments arranged by size:
1345 1029 1023 391 174 36 27
EagI C'GGCC_G
Cuts at: 545 581 1610 545
Size: 36 1029 2960
Fragments arranged by size:
2960 1029 36
EarI CTCTTCn'nnn_
Cuts at: 441 777 2048 2258 441
Size: 336 1271 210 2208
Fragments arranged by size:
2208 1271 336 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 664 2233 2543 2689 3835 664
Size: 1569 310 146 1146 854
Fragments arranged by size:
1569 1146 854 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 778 1850 2282 3029 3297 772
Size: 6 1072 432 747 268 1500
Fragments arranged by size:
1500 1072 747 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 1164 1164
Size: 4025
EcoRI G'AATT_C
Cuts at: 766 1029 766
Size: 263 3762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 482 1806 482
Size: 1324 2701
FspAI rTGC'GCAy
Cuts at: 482 482
Size: 4025
HaeII r_GCGC'y
Cuts at: 341 1459 1707 2729 3708 341
Size: 1118 248 1022 979 658
Fragments arranged by size:
1118 1022 979 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 894 926 955 961 987 993 1867 1897 1899
Size: 32 29 6 26 6 874 30 2
Cuts at: 1899 1929 894
Size: 30 2990
Fragments arranged by size:
2990 874 32 30 30 29 26 6
6 2
HincII GTy'rAC
Cuts at: 129 1064 129
Size: 935 3090
KasI G'GCGC_C
Cuts at: 337 1455 1703 337
Size: 1118 248 2659
Fragments arranged by size:
2659 1118 248
KpnI G_GTAC'C
Cuts at: 706 1045 706
Size: 339 3686
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 800 812 2696 2880 800
Size: 12 1884 184 1945
Fragments arranged by size:
1945 1884 184 12
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 2014 2141 2014
Size: 127 3898
MspA1I CmG'CkG
Cuts at: 347 468 550 571 586 657 1136 1450 1810
Size: 121 82 21 15 71 479 314 360
Cuts at: 1810 2823 3068 347
Size: 1013 245 1304
Fragments arranged by size:
1304 1013 479 360 314 245 121 82
71 21 15
NaeI GCC'GGC
Cuts at: 342 983 2206 342
Size: 641 1223 2161
Fragments arranged by size:
2161 1223 641
NarI GG'CG_CC
Cuts at: 338 1456 1704 338
Size: 1118 248 2659
Fragments arranged by size:
2659 1118 248
NcoI C'CATG_G
Cuts at: 677 677
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 340 981 2204 340
Size: 641 1223 2161
Fragments arranged by size:
2161 1223 641
NotI GC'GGCC_GC
Cuts at: 581 581
Size: 4025
NspI r_CATG'y
Cuts at: 219 783 1078 1578 2485 219
Size: 564 295 500 907 1759
Fragments arranged by size:
1759 907 564 500 295
PciI A'CATG_T
Cuts at: 779 2481 779
Size: 1702 2323
PfoI T'CCnGG_A
Cuts at: 749 749
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PstI C_TGCA'G
Cuts at: 1072 1757 1072
Size: 685 3340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SacII CC_GC'GG
Cuts at: 469 551 587 658 469
Size: 82 36 71 3836
Fragments arranged by size:
3836 82 71 36
SalI G'TCGA_C
Cuts at: 127 1062 127
Size: 935 3090
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 1068 1753 2746 2937 33
Size: 1035 685 993 191 1121
Fragments arranged by size:
1121 1035 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 589 589
Size: 4025
SfoI GGC'GCC
Cuts at: 339 1457 1705 339
Size: 1118 248 2659
Fragments arranged by size:
2659 1118 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 189 1047 189
Size: 858 3167
SmlI C'TyrA_G
Cuts at: 421 1180 2095 2587 2849 421
Size: 759 915 492 262 1597
Fragments arranged by size:
1597 915 759 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 219 1078 1578 219
Size: 859 500 2666
Fragments arranged by size:
2666 859 500
StyI C'CwwG_G
Cuts at: 593 677 1159 1513 593
Size: 84 482 354 3105
Fragments arranged by size:
3105 482 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 3542 3574 301
Size: 32 3209 32 752
Fragments arranged by size:
3209 752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 706 1520 642
Size: 64 814 3147
Fragments arranged by size:
3147 814 64
XmaI C'CCGG_G
Cuts at: 187 1045 187
Size: 858 3167
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI