Restriction map for
pF1KB8942
(Circular) (Six-base) MAPSORT of: pF1KB8942.seq Check: 9348 from: 1 to: 4016
pF1KB8942 4016 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 798 889 525
Size: 273 91 3652
Fragments arranged by size:
3652 273 91
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 572 1032 572
Size: 460 3556
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1841 2273 3020 3288 1841
Size: 432 747 268 2569
Fragments arranged by size:
2569 747 432 268
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 237 540 1007 1321 2888 237
Size: 303 467 314 1567 1365
Fragments arranged by size:
1567 1365 467 314 303
ApaI G_GGCC'C
Cuts at: 775 782 775
Size: 7 4009
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 1020 3369 3625 1020
Size: 2349 256 1411
Fragments arranged by size:
2349 1411 256
AscI GG'CGCG_CC
Cuts at: 893 893
Size: 4016
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 586 775 900 1036 586
Size: 189 125 136 3566
Fragments arranged by size:
3566 189 136 125
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 426 572 877 1032 1446 1694 1729 426
Size: 146 305 155 414 248 35 2713
Fragments arranged by size:
2713 414 305 248 155 146 35
BanII G_rGCy'C
Cuts at: 121 220 775 782 1030 2060 121
Size: 99 555 7 248 1030 2077
Fragments arranged by size:
2077 1030 555 248 99 7
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 803 837 90
Size: 34 679 34 3269
Fragments arranged by size:
3269 679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglII A'GATC_T
Cuts at: 638 1531 3568 638
Size: 893 2037 1086
Fragments arranged by size:
2037 1086 893
BlpI GC'TnA_GC
Cuts at: 115 1128 115
Size: 1013 3003
Bme1580I G_kGCm'C
Cuts at: 740 775 782 1641 1734 2790 740
Size: 35 7 859 93 1056 1966
Fragments arranged by size:
1966 1056 859 93 35 7
BmgBI CAC'GTC
Cuts at: 623 623
Size: 4016
BmrI ACTGGGnnnn_n'
Cuts at: 1228 1309 1644 3788 1228
Size: 81 335 2144 1456
Fragments arranged by size:
2144 1456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 568 184
Size: 384 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2563 2861 1192
Size: 879 492 298 2347
Fragments arranged by size:
2347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 1369 1369
Size: 4016
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 799 1441 2199 2776 3272 799
Size: 642 758 577 496 1543
Fragments arranged by size:
1543 758 642 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 833
Size: 4016
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 521 599 858 1030 1808 1998 2790 521
Size: 78 259 172 778 190 792 1747
Fragments arranged by size:
1747 792 778 259 190 172 78
BsmI GAATG_Cn'
Cuts at: 963 963
Size: 4016
Bsp1286I G_dGCh'C
Cuts at: 121 220 521 599 740 775 782 858 1030
Size: 99 301 78 141 35 7 76 172
Cuts at: 1030 1641 1734 1808 1998 2060 2790 121
Size: 611 93 74 190 62 730 1347
Fragments arranged by size:
1347 730 611 301 190 172 141 99
93 78 76 74 62 35 7
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 621 798 889 1066 1280 1582 1963 525
Size: 96 177 91 177 214 302 381 2578
Fragments arranged by size:
2578 381 302 214 177 177 96 91
BsrBI CCG'CTC
Cuts at: 50 174 476 552 2308 2362 3846 4011 50
Size: 124 302 76 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 302 165 124 76 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssHII G'CGCG_C
Cuts at: 608 893 608
Size: 285 3731
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 764 1393 764
Size: 629 3387
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 638 1041 1508 1531 1866 2112 3124 3210
Size: 637 403 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 637 467 449 403 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 185 202 185
Size: 17 3999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 767 1966 2147 2310 767
Size: 1199 181 163 2473
Fragments arranged by size:
2473 1199 181 163
BtsI GCAGTG_nn'
Cuts at: 824 1259 824
Size: 435 3581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 264 299 264
Size: 35 3981
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 471 602 705 926 951 1601 1775 2166 2193
Size: 131 103 221 25 650 174 391 27
Cuts at: 2193 3538 471
Size: 1345 949
Fragments arranged by size:
1345 949 650 391 221 174 131 103
27 25
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 208 2039 2249 208
Size: 1831 210 1975
Fragments arranged by size:
1975 1831 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 568 1841 2273 3020 3288 184
Size: 384 1273 432 747 268 912
Fragments arranged by size:
1273 912 747 432 384 268
EcoO109I rG'GnC_Cy
Cuts at: 331 772 904 1004 1155 331
Size: 441 132 100 151 3192
Fragments arranged by size:
3192 441 151 132 100
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 558 586 681 1450 1698 2720 3699 558
Size: 28 95 769 248 1022 979 875
Fragments arranged by size:
1022 979 875 769 248 95 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1858 1888 1890 1920 1858
Size: 30 2 30 3954
Fragments arranged by size:
3954 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 576 1036 576
Size: 460 3556
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2871 2687
Size: 184 3832
MscI TGG'CCA
Cuts at: 604 707 928 953 1777 604
Size: 103 221 25 824 2843
Fragments arranged by size:
2843 824 221 103 25
MslI CAynn'nnrTG
Cuts at: 2005 2132 2005
Size: 127 3889
MspA1I CmG'CkG
Cuts at: 187 204 452 570 851 1127 1441 1801 2814
Size: 17 248 118 281 276 314 360 1013
Cuts at: 2814 3059 187
Size: 245 1144
Fragments arranged by size:
1144 1013 360 314 281 276 248 245
118 17
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 1069 1569 2476 1069
Size: 500 907 2609
Fragments arranged by size:
2609 907 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 385 417 3155 3187 385
Size: 32 2738 32 1214
Fragments arranged by size:
2738 1214 32 32
PpuMI rG'GwC_Cy
Cuts at: 331 904 331
Size: 573 3443
PspOMI G'GGCC_C
Cuts at: 771 778 771
Size: 7 4009
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 643 675 643
Size: 32 3984
PstI C_TGCA'G
Cuts at: 617 995 1063 1748 617
Size: 378 68 685 2885
Fragments arranged by size:
2885 685 378 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SacII CC_GC'GG
Cuts at: 188 205 188
Size: 17 3999
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SanDI GG'GwC_CC
Cuts at: 331 331
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 208 2039 2249 208
Size: 1831 210 1975
Fragments arranged by size:
1975 1831 210
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 613 807 846 991 1059 1744 2737 2928
Size: 580 194 39 145 68 685 993 191
Cuts at: 2928 33
Size: 1121
Fragments arranged by size:
1121 993 685 580 194 191 145 68
39
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 777 1038 777
Size: 261 3755
SmlI C'TyrA_G
Cuts at: 1171 2086 2578 2840 1171
Size: 915 492 262 2347
Fragments arranged by size:
2347 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 488 1150 1504 488
Size: 662 354 3000
Fragments arranged by size:
3000 662 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 132 1599 1732 2191 2400 132
Size: 1467 133 459 209 1748
Fragments arranged by size:
1748 1467 459 209 133
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 779 811 3533 3565 779
Size: 32 2722 32 1230
Fragments arranged by size:
2722 1230 32 32
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 775 1036 775
Size: 261 3755
XmnI GAAnn'nnTTC
Cuts at: 227 227
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI