Restriction map for
pF1KB8938
(Circular) (Six-base) MAPSORT of: pF1KB8938.seq Check: 3212 from: 1 to: 3983
pF1KB8938 3983 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 696 1021 696
Size: 325 3658
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 691 1288 2855 691
Size: 597 1567 1819
Fragments arranged by size:
1819 1567 597
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 751 886 987 3336 3592 751
Size: 135 101 2349 256 1142
Fragments arranged by size:
2349 1142 256 135 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 220 1003 220
Size: 783 3200
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 999 1413 1661 1696 999
Size: 414 248 35 3286
Fragments arranged by size:
3286 414 248 35
BanII G_rGCy'C
Cuts at: 506 646 997 2027 506
Size: 140 351 1030 2462
Fragments arranged by size:
2462 1030 351 140
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 714 959 714
Size: 245 3738
BciVI GTATCCnnnnn_n'
Cuts at: 514 1881 2642 3983 514
Size: 1367 761 1341 514
Fragments arranged by size:
1367 1341 761 514
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglI GCCn_nnn'nGGC
Cuts at: 424 424
Size: 3983
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 359 1195 1276 1611 3755 359
Size: 836 81 335 2144 587
Fragments arranged by size:
2144 836 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 665 2350 665
Size: 1685 2298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 669 3687 669
Size: 3018 965
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1172 1693 2371 2645 2792 3203 1172
Size: 521 678 274 147 411 1952
Fragments arranged by size:
1952 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 428 431 428
Size: 3 3980
BseYI C'CCAG_C
Cuts at: 557 1408 2166 2743 3239 557
Size: 851 758 577 496 1301
Fragments arranged by size:
1301 851 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 234 413 1525 1571 2779 104
Size: 130 179 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 179 130 46
BsiHKAI G_wGCw'C
Cuts at: 646 997 1775 1965 2757 646
Size: 351 778 190 792 1872
Fragments arranged by size:
1872 792 778 351 190
BsmBI CGTCTCn'nnnn_
Cuts at: 653 653
Size: 3983
Bsp1286I G_dGCh'C
Cuts at: 506 646 997 1608 1701 1775 1965 2027 2757
Size: 140 351 611 93 74 190 62 730
Cuts at: 2757 506
Size: 1732
Fragments arranged by size:
1732 730 611 351 190 140 93 74
62
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 283 1981 2162 2371 283
Size: 1698 181 209 1895
Fragments arranged by size:
1895 1698 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstYI r'GATC_y
Cuts at: 1 1008 1475 1498 1833 2079 3091 3177 3189
Size: 1007 467 23 335 246 1012 86 12
Cuts at: 3189 3535 1
Size: 346 449
Fragments arranged by size:
1012 1007 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 667 667
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 231 410 1568 1742 2133 2160 3505 231
Size: 179 1158 174 391 27 1345 709
Fragments arranged by size:
1345 1158 709 391 179 174 27
EagI C'GGCC_G
Cuts at: 231 410 1568 231
Size: 179 1158 2646
Fragments arranged by size:
2646 1158 179
EarI CTCTTCn'nnn_
Cuts at: 696 2006 2216 696
Size: 1310 210 2463
Fragments arranged by size:
2463 1310 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 443 449 2191 2501 2647 3793 443
Size: 6 1742 310 146 1146 633
Fragments arranged by size:
1742 1146 633 310 146 6
EcoICRI GAG'CTC
Cuts at: 644 995 644
Size: 351 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1808 2240 2987 3255 967
Size: 841 432 747 268 1695
Fragments arranged by size:
1695 841 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 621 1122 621
Size: 501 3482
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 281 1417 1665 2687 3666 281
Size: 1136 248 1022 979 598
Fragments arranged by size:
1136 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 580 612 1825 1855 1857 1887 477
Size: 32 71 32 1213 30 2 30 2573
Fragments arranged by size:
2573 1213 71 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 2654 2838 722
Size: 1932 184 1867
Fragments arranged by size:
1932 1867 184
MscI TGG'CCA
Cuts at: 1744 1744
Size: 3983
MslI CAynn'nnrTG
Cuts at: 1972 2099 1972
Size: 127 3856
MspA1I CmG'CkG
Cuts at: 132 415 1094 1408 1768 2781 3026 132
Size: 283 679 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 679 360 314 283 245
NaeI GCC'GGC
Cuts at: 285 2164 285
Size: 1879 2104
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NgoMIV G'CCGG_C
Cuts at: 283 2162 283
Size: 1879 2104
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 945 945
Size: 3983
PfoI T'CCnGG_A
Cuts at: 343 343
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PpuMI rG'GwC_Cy
Cuts at: 621 621
Size: 3983
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 118 150 118
Size: 32 3951
PstI C_TGCA'G
Cuts at: 263 1030 1715 263
Size: 767 685 2531
Fragments arranged by size:
2531 767 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 646 997 646
Size: 351 3632
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 385 3500 385
Size: 3115 868
SfcI C'TryA_G
Cuts at: 33 259 717 1026 1711 2704 2895 33
Size: 226 458 309 685 993 191 1121
Fragments arranged by size:
1121 993 685 458 309 226 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
SspI AAT'ATT
Cuts at: 612 612
Size: 3983
StuI AGG'CCT
Cuts at: 968 968
Size: 3983
StyI C'CwwG_G
Cuts at: 1117 1471 1117
Size: 354 3629
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 341 1566 1699 2158 2367 341
Size: 1225 133 459 209 1957
Fragments arranged by size:
1957 1225 459 209 133
TatI w'GTAC_w
Cuts at: 134 143 383 3498 134
Size: 9 240 3115 619
Fragments arranged by size:
3115 619 240 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 735 1014 735
Size: 279 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI