Restriction map for
pF1KB8935
(Circular) (Six-base) MAPSORT of: pF1KB8935.seq Check: 5306 from: 1 to: 3965
pF1KB8935 3965 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2969 3237 1790
Size: 432 747 268 2518
Fragments arranged by size:
2518 747 432 268
AflIII A'CryG_T
Cuts at: 841 1317 2421 3398 841
Size: 476 1104 977 1408
Fragments arranged by size:
1408 1104 977 476
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AlwNI CAG_nnn'CTG
Cuts at: 1270 2837 1270
Size: 1567 2398
ApaLI G'TGCA_C
Cuts at: 2735 2735
Size: 3965
ApoI r'AATT_y
Cuts at: 669 969 3318 3574 669
Size: 300 2349 256 1060
Fragments arranged by size:
2349 1060 300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 422 449 985 422
Size: 27 536 3402
Fragments arranged by size:
3402 536 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 419 452 419
Size: 33 3932
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 453 981 1395 1643 1678 453
Size: 528 414 248 35 2740
Fragments arranged by size:
2740 528 414 248 35
BanII G_rGCy'C
Cuts at: 979 2009 979
Size: 1030 2935
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 326 360 326
Size: 34 3931
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BglII A'GATC_T
Cuts at: 1480 3517 1480
Size: 2037 1928
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 456 1590 1683 2739 456
Size: 1134 93 1056 1682
Fragments arranged by size:
1682 1134 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1177 1258 1593 3737 1177
Size: 81 335 2144 1405
Fragments arranged by size:
2144 1405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2512 2810 1141
Size: 879 492 298 2296
Fragments arranged by size:
2296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3669 3669
Size: 3965
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 1479 3516 6
Size: 1473 2037 455
Fragments arranged by size:
2037 1473 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1154 1675 2353 2627 2774 3185 1154
Size: 521 678 274 147 411 1934
Fragments arranged by size:
1934 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1390 2148 2725 3221 1390
Size: 758 577 496 2134
Fragments arranged by size:
2134 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 496
Size: 3965
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2761 104
Size: 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 979 1757 1947 2739 979
Size: 778 190 792 2205
Fragments arranged by size:
2205 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 456 979 1590 1683 1757 1947 2009 2739 456
Size: 523 611 93 74 190 62 730 1682
Fragments arranged by size:
1682 730 611 523 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3185 3185
Size: 3965
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 342 1015 1229 1531 1912 342
Size: 673 214 302 381 2395
Fragments arranged by size:
2395 673 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2257 2311 3795 3960 50
Size: 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 530 1877 396
Size: 134 1347 2484
Fragments arranged by size:
2484 1347 134
BsrFI r'CCGG_y
Cuts at: 1963 2144 2353 1963
Size: 181 209 3575
Fragments arranged by size:
3575 209 181
BssSI C'ACGA_G
Cuts at: 2236 2594 2236
Size: 358 3607
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 1342
Size: 3965
BstBI TT'CG_AA
Cuts at: 513 2326 513
Size: 1813 2152
BstXI CCAn_nnnn'nTGG
Cuts at: 141 141
Size: 3965
BstYI r'GATC_y
Cuts at: 1 562 990 1457 1480 1815 2061 3073 3159
Size: 561 428 467 23 335 246 1012 86
Cuts at: 3159 3171 3517 1
Size: 12 346 449
Fragments arranged by size:
1012 561 467 449 428 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 359 359
Size: 3965
BtgI C'CryG_G
Cuts at: 107 107
Size: 3965
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 329 1915 2096 2259 329
Size: 1586 181 163 2035
Fragments arranged by size:
2035 1586 181 163
BtsI GCAGTG_nn'
Cuts at: 1208 1208
Size: 3965
ClaI AT'CG_AT
Cuts at: 945 945
Size: 3965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 798 833 798
Size: 35 3930
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 121 1671 2529 121
Size: 1550 858 1557
Fragments arranged by size:
1557 1550 858
EaeI y'GGCC_r
Cuts at: 806 1550 1724 2115 2142 3487 806
Size: 744 174 391 27 1345 1284
Fragments arranged by size:
1345 1284 744 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 515 1988 2198 515
Size: 1473 210 2282
Fragments arranged by size:
2282 1473 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2173 2483 2629 3775 2173
Size: 310 146 1146 2363
Fragments arranged by size:
2363 1146 310 146
EcoICRI GAG'CTC
Cuts at: 977 977
Size: 3965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2969 3237 1790
Size: 432 747 268 2518
Fragments arranged by size:
2518 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 821 1104 821
Size: 283 3682
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
EcoRV GAT'ATC
Cuts at: 641 641
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 1399 1647 2669 3648 1399
Size: 248 1022 979 1716
Fragments arranged by size:
1716 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 405 437 630 662 1807 1837 1839
Size: 32 93 32 193 32 1145 30 2
Cuts at: 1839 1869 280
Size: 30 2376
Fragments arranged by size:
2376 1145 193 93 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MfeI C'AATT_G
Cuts at: 536 536
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 808 1726 808
Size: 918 3047
MslI CAynn'nnrTG
Cuts at: 1954 2081 1954
Size: 127 3838
MspA1I CmG'CkG
Cuts at: 386 1076 1390 1750 2763 3008 386
Size: 690 314 360 1013 245 1343
Fragments arranged by size:
1343 1013 690 360 314 245
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 107 107
Size: 3965
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NspI r_CATG'y
Cuts at: 845 1018 1518 2425 845
Size: 173 500 907 2385
Fragments arranged by size:
2385 907 500 173
PciI A'CATG_T
Cuts at: 841 2421 841
Size: 1580 2385
PfoI T'CCnGG_A
Cuts at: 271 271
Size: 3965
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PpuMI rG'GwC_Cy
Cuts at: 821 821
Size: 3965
PstI C_TGCA'G
Cuts at: 182 507 1012 1697 182
Size: 325 505 685 2450
Fragments arranged by size:
2450 685 505 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 1390 1750 1390
Size: 360 3605
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 979 979
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SanDI GG'GwC_CC
Cuts at: 821 821
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 515 1988 2198 515
Size: 1473 210 2282
Fragments arranged by size:
2282 1473 210
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 378 3482 378
Size: 3104 861
SfcI C'TryA_G
Cuts at: 33 178 503 1008 1693 2686 2877 33
Size: 145 325 505 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 325 191 145
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 422 1120 2035 2527 2789 422
Size: 698 915 492 262 1598
Fragments arranged by size:
1598 915 698 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
SspI AAT'ATT
Cuts at: 156 699 156
Size: 543 3422
StyI C'CwwG_G
Cuts at: 107 1099 1453 107
Size: 992 354 2619
Fragments arranged by size:
2619 992 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1548 1681 2140 2349 1548
Size: 133 459 209 3164
Fragments arranged by size:
3164 459 209 133
TatI w'GTAC_w
Cuts at: 226 376 3480 226
Size: 150 3104 711
Fragments arranged by size:
3104 711 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 942 974 3482 3514 942
Size: 32 2508 32 1393
Fragments arranged by size:
2508 1393 32 32
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1460 1460
Size: 3965
XhoI C'TCGA_G
Cuts at: 422 422
Size: 3965
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I DraIII EcoNI FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI