Restriction map for
pF1KB8934
(Circular) (Six-base) MAPSORT of: pF1KB8934.seq Check: 6955 from: 1 to: 3965
pF1KB8934 3965 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 328 328
Size: 3965
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2969 3237 1790
Size: 432 747 268 2518
Fragments arranged by size:
2518 747 432 268
AfeI AGC'GCT
Cuts at: 951 951
Size: 3965
AflIII A'CryG_T
Cuts at: 542 1317 2421 3398 542
Size: 775 1104 977 1109
Fragments arranged by size:
1109 1104 977 775
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AlwNI CAG_nnn'CTG
Cuts at: 561 1270 2837 561
Size: 709 1567 1689
Fragments arranged by size:
1689 1567 709
ApaLI G'TGCA_C
Cuts at: 2735 2735
Size: 3965
ApoI r'AATT_y
Cuts at: 969 3318 3574 969
Size: 2349 256 1360
Fragments arranged by size:
2349 1360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 985 985
Size: 3965
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 222 312 981 1395 1643 1678 222
Size: 90 669 414 248 35 2509
Fragments arranged by size:
2509 669 414 248 90 35
BanII G_rGCy'C
Cuts at: 116 394 627 979 2009 116
Size: 278 233 352 1030 2072
Fragments arranged by size:
2072 1030 352 278 233
BbeI G_GCGC'C
Cuts at: 226 1399 1647 226
Size: 1173 248 2544
Fragments arranged by size:
2544 1173 248
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BglI GCCn_nnn'nGGC
Cuts at: 424 424
Size: 3965
BglII A'GATC_T
Cuts at: 851 1480 3517 851
Size: 629 2037 1299
Fragments arranged by size:
2037 1299 629
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 317 411 1590 1683 2739 317
Size: 94 1179 93 1056 1543
Fragments arranged by size:
1543 1179 1056 94 93
BmgBI CAC'GTC
Cuts at: 543 543
Size: 3965
BmrI ACTGGGnnnn_n'
Cuts at: 1177 1258 1593 3737 1177
Size: 81 335 2144 1405
Fragments arranged by size:
2144 1405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 696 820 593
Size: 103 124 3738
Fragments arranged by size:
3738 124 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2512 2810 1141
Size: 879 492 298 2296
Fragments arranged by size:
2296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3669 3669
Size: 3965
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 889 1479 3516 6
Size: 883 590 2037 455
Fragments arranged by size:
2037 883 590 455
BsaHI Gr'CG_yC
Cuts at: 223 325 734 1396 1644 2346 3842 223
Size: 102 409 662 248 702 1496 346
Fragments arranged by size:
1496 702 662 409 346 248 102
BsaWI w'CCGG_w
Cuts at: 1154 1675 2353 2627 2774 3185 1154
Size: 521 678 274 147 411 1934
Fragments arranged by size:
1934 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 793 823 793
Size: 30 3935
BseYI C'CCAG_C
Cuts at: 162 216 330 469 1390 2148 2725 3221 162
Size: 54 114 139 921 758 577 496 906
Fragments arranged by size:
921 906 758 577 496 139 114 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 574
Size: 3965
BsiEI CG_ry'CG
Cuts at: 104 455 756 1507 1553 2761 104
Size: 351 301 751 46 1208 1308
Fragments arranged by size:
1308 1208 751 351 301 46
BsiHKAI G_wGCw'C
Cuts at: 394 570 979 1757 1947 2739 394
Size: 176 409 778 190 792 1620
Fragments arranged by size:
1620 792 778 409 190 176
BsmI GAATG_Cn'
Cuts at: 767 767
Size: 3965
Bsp1286I G_dGCh'C
Cuts at: 116 317 394 411 570 627 979 1590 1683
Size: 201 77 17 159 57 352 611 93
Cuts at: 1683 1757 1947 2009 2739 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 352 201 190 159 93
77 74 62 57 17
BspEI T'CCGG_A
Cuts at: 3185 3185
Size: 3965
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1015 1229 1531 1912 1015
Size: 214 302 381 3068
Fragments arranged by size:
3068 381 302 214
BsrBI CCG'CTC
Cuts at: 50 169 804 2257 2311 3795 3960 50
Size: 119 635 1453 54 1484 165 55
Fragments arranged by size:
1484 1453 635 165 119 55 54
BsrDI GCAATG_nn'
Cuts at: 298 1877 298
Size: 1579 2386
BsrFI r'CCGG_y
Cuts at: 1963 2144 2353 1963
Size: 181 209 3575
Fragments arranged by size:
3575 209 181
BssSI C'ACGA_G
Cuts at: 2236 2594 2236
Size: 358 3607
BstAPI GCAn_nnn'nTGC
Cuts at: 703 1342 703
Size: 639 3326
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 537 537
Size: 3965
BstYI r'GATC_y
Cuts at: 1 261 851 990 1457 1480 1815 2061 3073
Size: 260 590 139 467 23 335 246 1012
Cuts at: 3073 3159 3171 3517 1
Size: 86 12 346 449
Fragments arranged by size:
1012 590 467 449 346 335 260 246
139 86 23 12
BtgI C'CryG_G
Cuts at: 107 437 449 107
Size: 330 12 3623
Fragments arranged by size:
3623 330 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 1208 1208
Size: 3965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 468 503 468
Size: 35 3930
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 1671 2529 1671
Size: 858 3107
EaeI y'GGCC_r
Cuts at: 425 452 527 753 1550 1724 2115 2142 3487
Size: 27 75 226 797 174 391 27 1345
Cuts at: 3487 425
Size: 903
Fragments arranged by size:
1345 903 797 391 226 174 75 27
27
EagI C'GGCC_G
Cuts at: 452 753 1550 452
Size: 301 797 2867
Fragments arranged by size:
2867 797 301
EarI CTCTTCn'nnn_
Cuts at: 455 1988 2198 455
Size: 1533 210 2222
Fragments arranged by size:
2222 1533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 195 292 2173 2483 2629 3775 195
Size: 97 1881 310 146 1146 385
Fragments arranged by size:
1881 1146 385 310 146 97
EcoICRI GAG'CTC
Cuts at: 392 977 392
Size: 585 3380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 696 820 1790 2222 2969 3237 593
Size: 103 124 970 432 747 268 1321
Fragments arranged by size:
1321 970 747 432 268 124 103
EcoO109I rG'GnC_Cy
Cuts at: 682 1104 682
Size: 422 3543
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
EcoRV GAT'ATC
Cuts at: 150 505 150
Size: 355 3610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 226 882 953 1399 1647 2669 3648 226
Size: 656 71 446 248 1022 979 543
Fragments arranged by size:
1022 979 656 543 446 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1807 1837 1839 1869 1807
Size: 30 2 30 3903
Fragments arranged by size:
3903 30 30 2
HincII GTy'rAC
Cuts at: 246 1004 246
Size: 758 3207
KasI G'GCGC_C
Cuts at: 222 1395 1643 222
Size: 1173 248 2544
Fragments arranged by size:
2544 1173 248
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 529 1726 529
Size: 1197 2768
MslI CAynn'nnrTG
Cuts at: 1954 2081 1954
Size: 127 3838
MspA1I CmG'CkG
Cuts at: 220 439 716 1076 1390 1750 2763 3008 220
Size: 219 277 360 314 360 1013 245 1177
Fragments arranged by size:
1177 1013 360 360 314 277 245 219
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 223 1396 1644 223
Size: 1173 248 2544
Fragments arranged by size:
2544 1173 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3965
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NotI GC'GGCC_GC
Cuts at: 753 753
Size: 3965
NspI r_CATG'y
Cuts at: 1018 1518 2425 1018
Size: 500 907 2558
Fragments arranged by size:
2558 907 500
PasI CC'CwG_GG
Cuts at: 307 318 307
Size: 11 3954
PciI A'CATG_T
Cuts at: 2421 2421
Size: 3965
PflMI CCAn_nnn'nTGG
Cuts at: 307 525 307
Size: 218 3747
PfoI T'CCnGG_A
Cuts at: 385 498 507 570 385
Size: 113 9 63 3780
Fragments arranged by size:
3780 113 63 9
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PpuMI rG'GwC_Cy
Cuts at: 682 682
Size: 3965
PstI C_TGCA'G
Cuts at: 1012 1697 1012
Size: 685 3280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 1390 1750 1390
Size: 360 3605
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 394 979 394
Size: 585 3380
SacII CC_GC'GG
Cuts at: 440 440
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SexAI A'CCwGG_T
Cuts at: 678 678
Size: 3965
SfcI C'TryA_G
Cuts at: 33 1008 1693 2686 2877 33
Size: 975 685 993 191 1121
Fragments arranged by size:
1121 993 975 685 191
SfoI GGC'GCC
Cuts at: 224 1397 1645 224
Size: 1173 248 2544
Fragments arranged by size:
2544 1173 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 1120 2035 2527 2789 1120
Size: 915 492 262 2296
Fragments arranged by size:
2296 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 107 131 143 257 833 935 1099 1453 107
Size: 24 12 114 576 102 164 354 2619
Fragments arranged by size:
2619 576 354 164 114 102 24 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 230 314 553 1548 1681 2140 2349 230
Size: 84 239 995 133 459 209 1846
Fragments arranged by size:
1846 995 459 239 209 133 84
TatI w'GTAC_w
Cuts at: 3480 3480
Size: 3965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 304 686 1460 304
Size: 382 774 2809
Fragments arranged by size:
2809 774 382
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
ZraI GAC'GTC
Cuts at: 326 326
Size: 3965
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI