Restriction map for
pF1KB8931
(Circular) (Six-base) MAPSORT of: pF1KB8931.seq Check: 3535 from: 1 to: 3959
pF1KB8931 3959 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 728 975 728
Size: 247 3712
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1784 2216 2963 3231 448
Size: 1336 432 747 268 1176
Fragments arranged by size:
1336 1176 747 432 268
AfeI AGC'GCT
Cuts at: 476 476
Size: 3959
AflIII A'CryG_T
Cuts at: 254 1311 2415 3392 254
Size: 1057 1104 977 821
Fragments arranged by size:
1104 1057 977 821
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 603 1264 2831 603
Size: 661 1567 1731
Fragments arranged by size:
1731 1567 661
ApaI G_GGCC'C
Cuts at: 467 467
Size: 3959
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AscI GG'CGCG_CC
Cuts at: 719 719
Size: 3959
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 133 217 453 466 979 133
Size: 84 236 13 513 3113
Fragments arranged by size:
3113 513 236 84 13
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 649 728 975 1389 1637 1672 649
Size: 79 247 414 248 35 2936
Fragments arranged by size:
2936 414 248 247 79 35
BanII G_rGCy'C
Cuts at: 467 707 973 2003 467
Size: 240 266 1030 2423
Fragments arranged by size:
2423 1030 266 240
BbeI G_GCGC'C
Cuts at: 653 1393 1641 653
Size: 740 248 2971
Fragments arranged by size:
2971 740 248
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BglI GCCn_nnn'nGGC
Cuts at: 498 498
Size: 3959
BglII A'GATC_T
Cuts at: 884 1474 3511 884
Size: 590 2037 1332
Fragments arranged by size:
2037 1332 590
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 467 1584 1677 2733 467
Size: 1117 93 1056 1693
Fragments arranged by size:
1693 1117 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 316 1171 1252 1587 3731 316
Size: 855 81 335 2144 544
Fragments arranged by size:
2144 855 544 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 600 814 508
Size: 92 214 3653
Fragments arranged by size:
3653 214 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3663 3663
Size: 3959
BsaAI yAC'GTr
Cuts at: 353 1312 353
Size: 959 3000
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 650 1390 1638 2340 3836 650
Size: 740 248 702 1496 773
Fragments arranged by size:
1496 773 740 702 248
BsaWI w'CCGG_w
Cuts at: 899 1148 1669 2347 2621 2768 3179 899
Size: 249 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 574 604 787 817 244
Size: 30 300 30 183 30 3386
Fragments arranged by size:
3386 300 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 451
Size: 3959
BseYI C'CCAG_C
Cuts at: 1384 2142 2719 3215 1384
Size: 758 577 496 2128
Fragments arranged by size:
2128 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 348 1501 1547 2755 104
Size: 244 1153 46 1208 1308
Fragments arranged by size:
1308 1208 1153 244 46
BsiHKAI G_wGCw'C
Cuts at: 262 973 1751 1941 2733 262
Size: 711 778 190 792 1488
Fragments arranged by size:
1488 792 778 711 190
Bsp1286I G_dGCh'C
Cuts at: 262 467 707 973 1584 1677 1751 1941 2003
Size: 205 240 266 611 93 74 190 62
Cuts at: 2003 2733 262
Size: 730 1488
Fragments arranged by size:
1488 730 611 266 240 205 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 867 1009 1223 1525 1906 867
Size: 142 214 302 381 2920
Fragments arranged by size:
2920 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 746 2251 2305 3789 3954 50
Size: 696 1505 54 1484 165 55
Fragments arranged by size:
1505 1484 696 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 1871 297
Size: 1574 2385
BsrFI r'CCGG_y
Cuts at: 652 1957 2138 2347 652
Size: 1305 181 209 2264
Fragments arranged by size:
2264 1305 209 181
BssHII G'CGCG_C
Cuts at: 364 719 364
Size: 355 3604
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 772 2320 772
Size: 1548 2411
BstYI r'GATC_y
Cuts at: 1 884 984 1451 1474 1809 2055 3067 3153
Size: 883 100 467 23 335 246 1012 86
Cuts at: 3153 3165 3511 1
Size: 12 346 449
Fragments arranged by size:
1012 883 467 449 346 335 246 100
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3959
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 877 1665 2523 877
Size: 788 858 2313
Fragments arranged by size:
2313 858 788
EaeI y'GGCC_r
Cuts at: 271 345 710 1544 1718 2109 2136 3481 271
Size: 74 365 834 174 391 27 1345 749
Fragments arranged by size:
1345 834 749 391 365 174 74 27
EagI C'GGCC_G
Cuts at: 345 1544 345
Size: 1199 2760
EarI CTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 2167 2477 2623 3769 295
Size: 1872 310 146 1146 485
Fragments arranged by size:
1872 1146 485 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 508 600 814 1784 2216 2963 3231 448
Size: 60 92 214 970 432 747 268 1176
Fragments arranged by size:
1176 970 747 432 268 214 92 60
EcoO109I rG'GnC_Cy
Cuts at: 423 571 1098 423
Size: 148 527 3284
Fragments arranged by size:
3284 527 148
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 478 653 837 1393 1641 2663 3642 478
Size: 175 184 556 248 1022 979 795
Fragments arranged by size:
1022 979 795 556 248 184 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1801 1831 1833 1863 574
Size: 32 1195 30 2 30 2670
Fragments arranged by size:
2670 1195 32 30 30 2
HincII GTy'rAC
Cuts at: 813 998 813
Size: 185 3774
KasI G'GCGC_C
Cuts at: 649 1389 1637 649
Size: 740 248 2971
Fragments arranged by size:
2971 740 248
KpnI G_GTAC'C
Cuts at: 732 979 732
Size: 247 3712
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 2630 2814 635
Size: 1995 184 1780
Fragments arranged by size:
1995 1780 184
MscI TGG'CCA
Cuts at: 1720 1720
Size: 3959
MslI CAynn'nnrTG
Cuts at: 1948 2075 1948
Size: 127 3832
MspA1I CmG'CkG
Cuts at: 150 486 716 1070 1384 1744 2757 3002 150
Size: 336 230 354 314 360 1013 245 1107
Fragments arranged by size:
1107 1013 360 354 336 314 245 230
NaeI GCC'GGC
Cuts at: 654 2140 654
Size: 1486 2473
NarI GG'CG_CC
Cuts at: 650 1390 1638 650
Size: 740 248 2971
Fragments arranged by size:
2971 740 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3959
NgoMIV G'CCGG_C
Cuts at: 652 2138 652
Size: 1486 2473
NspI r_CATG'y
Cuts at: 1012 1512 2419 1012
Size: 500 907 2552
Fragments arranged by size:
2552 907 500
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PflMI CCAn_nnn'nTGG
Cuts at: 603 603
Size: 3959
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PmlI CAC'GTG
Cuts at: 353 353
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PpuMI rG'GwC_Cy
Cuts at: 571 571
Size: 3959
PspOMI G'GGCC_C
Cuts at: 463 463
Size: 3959
PstI C_TGCA'G
Cuts at: 1006 1691 1006
Size: 685 3274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SfcI C'TryA_G
Cuts at: 33 1002 1687 2680 2871 33
Size: 969 685 993 191 1121
Fragments arranged by size:
1121 993 969 685 191
SfoI GGC'GCC
Cuts at: 651 1391 1639 651
Size: 740 248 2971
Fragments arranged by size:
2971 740 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SgrAI Cr'CCGG_yG
Cuts at: 652 652
Size: 3959
SmaI CCC'GGG
Cuts at: 135 219 455 468 981 135
Size: 84 236 13 513 3113
Fragments arranged by size:
3113 513 236 84 13
SmlI C'TyrA_G
Cuts at: 1114 2029 2521 2783 1114
Size: 915 492 262 2290
Fragments arranged by size:
2290 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
StyI C'CwwG_G
Cuts at: 107 1093 1447 107
Size: 986 354 2619
Fragments arranged by size:
2619 986 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1542 1675 2134 2343 1542
Size: 133 459 209 3158
Fragments arranged by size:
3158 459 209 133
TatI w'GTAC_w
Cuts at: 3474 3474
Size: 3959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 3927
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1454 1454
Size: 3959
XmaI C'CCGG_G
Cuts at: 133 217 453 466 979 133
Size: 84 236 13 513 3113
Fragments arranged by size:
3113 513 236 84 13
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI