Restriction map for
pF1KB8929
(Circular) (Six-base) MAPSORT of: pF1KB8929.seq Check: 9743 from: 1 to: 3956
pF1KB8929 3956 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 578 578
Size: 3956
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1781 2213 2960 3228 679
Size: 1102 432 747 268 1407
Fragments arranged by size:
1407 1102 747 432 268
AflIII A'CryG_T
Cuts at: 203 1308 2412 3389 203
Size: 1105 1104 977 770
Fragments arranged by size:
1105 1104 977 770
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 660 660
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 298 603 1261 2828 298
Size: 305 658 1567 1426
Fragments arranged by size:
1567 1426 658 305
ApaI G_GGCC'C
Cuts at: 188 322 188
Size: 134 3822
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 960 3309 3565 960
Size: 2349 256 1351
Fragments arranged by size:
2349 1351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 140 160 187 479 976 140
Size: 20 27 292 497 3120
Fragments arranged by size:
3120 497 292 27 20
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 663 972 1386 1634 1669 663
Size: 309 414 248 35 2950
Fragments arranged by size:
2950 414 309 248 35
BanII G_rGCy'C
Cuts at: 140 188 322 479 970 2000 140
Size: 48 134 157 491 1030 2096
Fragments arranged by size:
2096 1030 491 157 134 48
BbeI G_GCGC'C
Cuts at: 667 1390 1638 667
Size: 723 248 2985
Fragments arranged by size:
2985 723 248
BbvCI CC'TCA_GC
Cuts at: 733 733
Size: 3956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 262 296 735 769 262
Size: 34 439 34 3449
Fragments arranged by size:
3449 439 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglI GCCn_nnn'nGGC
Cuts at: 317 483 625 317
Size: 166 142 3648
Fragments arranged by size:
3648 166 142
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 188 322 1581 1674 2730 188
Size: 134 1259 93 1056 1414
Fragments arranged by size:
1414 1259 1056 134 93
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 838 817
Size: 21 3935
Bpu10I CC'TnA_GC
Cuts at: 733 733
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 598 742 1132 2011 2503 2801 298
Size: 300 144 390 879 492 298 1453
Fragments arranged by size:
1453 879 492 390 300 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 3660 3660
Size: 3956
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 664 1387 1635 2337 3833 664
Size: 723 248 702 1496 787
Fragments arranged by size:
1496 787 723 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 487
Size: 3956
BseYI C'CCAG_C
Cuts at: 181 411 1381 2139 2716 3212 181
Size: 230 970 758 577 496 925
Fragments arranged by size:
970 925 758 577 496 230
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 970 1748 1938 2730 970
Size: 778 190 792 2196
Fragments arranged by size:
2196 792 778 190
BsmI GAATG_Cn'
Cuts at: 791 791
Size: 3956
BsmBI CGTCTCn'nnnn_
Cuts at: 793 793
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 140 188 322 479 970 1581 1674 1748 1938
Size: 48 134 157 491 611 93 74 190
Cuts at: 1938 2000 2730 140
Size: 62 730 1366
Fragments arranged by size:
1366 730 611 491 190 157 134 93
74 62 48
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1006 1220 1522 1903 578
Size: 428 214 302 381 2631
Fragments arranged by size:
2631 428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1868 1868
Size: 3956
BsrFI r'CCGG_y
Cuts at: 728 1954 2135 2344 728
Size: 1226 181 209 2340
Fragments arranged by size:
2340 1226 209 181
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 813 1333 813
Size: 520 3436
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 3956
BstYI r'GATC_y
Cuts at: 1 981 1448 1471 1806 2052 3064 3150 3162
Size: 980 467 23 335 246 1012 86 12
Cuts at: 3162 3508 1
Size: 346 449
Fragments arranged by size:
1012 980 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 299 911 299
Size: 612 3344
BtgI C'CryG_G
Cuts at: 114 114
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 738 1906 2087 2250 738
Size: 1168 181 163 2444
Fragments arranged by size:
2444 1168 181 163
BtsI GCAGTG_nn'
Cuts at: 629 710 1199 629
Size: 81 489 3386
Fragments arranged by size:
3386 489 81
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DraIII CAC_nnn'GTG
Cuts at: 550 550
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 147 594 626 917 1541 1715 2106 2133 3478
Size: 447 32 291 624 174 391 27 1345
Cuts at: 3478 147
Size: 625
Fragments arranged by size:
1345 625 624 447 391 291 174 32
27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 251 901 1979 2189 251
Size: 650 1078 210 2018
Fragments arranged by size:
2018 1078 650 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 225 889 2164 2474 2620 3766 225
Size: 664 1275 310 146 1146 415
Fragments arranged by size:
1275 1146 664 415 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 817 838 1781 2213 2960 3228 679
Size: 138 21 943 432 747 268 1407
Fragments arranged by size:
1407 943 747 432 268 138 21
EcoNI CCTnn'n_nnAGG
Cuts at: 658 928 658
Size: 270 3686
EcoO109I rG'GnC_Cy
Cuts at: 119 318 319 415 618 1095 119
Size: 199 1 96 203 477 2980
Fragments arranged by size:
2980 477 203 199 96 1
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 280 736 1975 280
Size: 456 1239 2261
Fragments arranged by size:
2261 1239 456
FspI TGC'GCA
Cuts at: 763 1737 763
Size: 974 2982
HaeII r_GCGC'y
Cuts at: 298 412 667 843 1390 1638 2660 3639 298
Size: 114 255 176 547 248 1022 979 615
Fragments arranged by size:
1022 979 615 547 255 248 176 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 995 995
Size: 3956
KasI G'GCGC_C
Cuts at: 663 1386 1634 663
Size: 723 248 2985
Fragments arranged by size:
2985 723 248
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 149 628 1717 149
Size: 479 1089 2388
Fragments arranged by size:
2388 1089 479
MslI CAynn'nnrTG
Cuts at: 757 1945 2072 757
Size: 1188 127 2641
Fragments arranged by size:
2641 1188 127
MspA1I CmG'CkG
Cuts at: 1067 1381 1741 2754 2999 1067
Size: 314 360 1013 245 2024
Fragments arranged by size:
2024 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 664 1387 1635 664
Size: 723 248 2985
Fragments arranged by size:
2985 723 248
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NspI r_CATG'y
Cuts at: 207 1009 1509 2416 207
Size: 802 500 907 1747
Fragments arranged by size:
1747 907 802 500
PasI CC'CwG_GG
Cuts at: 436 452 436
Size: 16 3940
PciI A'CATG_T
Cuts at: 203 2412 203
Size: 2209 1747
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PpuMI rG'GwC_Cy
Cuts at: 119 119
Size: 3956
PspOMI G'GGCC_C
Cuts at: 184 318 184
Size: 134 3822
PspXI vC'TCGA_Gb
Cuts at: 160 160
Size: 3956
PstI C_TGCA'G
Cuts at: 533 649 1003 1688 533
Size: 116 354 685 2801
Fragments arranged by size:
2801 685 354 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 1381 1741 1381
Size: 360 3596
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 251 1979 2189 251
Size: 1728 210 2018
Fragments arranged by size:
2018 1728 210
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 426 529 645 999 1684 2677 2868 33
Size: 393 103 116 354 685 993 191 1121
Fragments arranged by size:
1121 993 685 393 354 191 116 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 317 625 317
Size: 308 3648
SfoI GGC'GCC
Cuts at: 665 1388 1636 665
Size: 723 248 2985
Fragments arranged by size:
2985 723 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 142 189 481 978 142
Size: 47 292 497 3120
Fragments arranged by size:
3120 497 292 47
SmlI C'TyrA_G
Cuts at: 160 313 613 721 1111 2026 2518 2780 160
Size: 153 300 108 390 915 492 262 1336
Fragments arranged by size:
1336 915 492 390 300 262 153 108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StuI AGG'CCT
Cuts at: 220 311 220
Size: 91 3865
StyI C'CwwG_G
Cuts at: 1090 1444 1090
Size: 354 3602
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 1539 1672 2131 2340 883
Size: 656 133 459 209 2499
Fragments arranged by size:
2499 656 459 209 133
TatI w'GTAC_w
Cuts at: 821 3471 821
Size: 2650 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 1451
Size: 3956
XhoI C'TCGA_G
Cuts at: 160 160
Size: 3956
XmaI C'CCGG_G
Cuts at: 140 187 479 976 140
Size: 47 292 497 3120
Fragments arranged by size:
3120 497 292 47
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI