Restriction map for
pF1KB8926
(Circular) (Six-base) MAPSORT of: pF1KB8926.seq Check: 4778 from: 1 to: 3932
pF1KB8926 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 948 948
Size: 3932
AclI AA'CG_TT
Cuts at: 385 3772 385
Size: 3387 545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 432 516 600 684 769 1757 2189 2936
Size: 223 84 84 84 85 988 432 747
Cuts at: 2936 3204 209
Size: 268 937
Fragments arranged by size:
988 937 747 432 268 223 85 84
84 84
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AhdI GACnn_n'nnGTC
Cuts at: 331 331
Size: 3932
AleI CACnn'nnGTG
Cuts at: 659 659
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaLI G'TGCA_C
Cuts at: 426 892 2702 426
Size: 466 1810 1656
Fragments arranged by size:
1810 1656 466
ApoI r'AATT_y
Cuts at: 249 936 3285 3541 249
Size: 687 2349 256 640
Fragments arranged by size:
2349 687 640 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 952 952
Size: 3932
BamHI G'GATC_C
Cuts at: 226 957 226
Size: 731 3201
BanI G'GyrC_C
Cuts at: 948 1362 1610 1645 948
Size: 414 248 35 3235
Fragments arranged by size:
3235 414 248 35
BanII G_rGCy'C
Cuts at: 946 1976 946
Size: 1030 2902
BbeI G_GCGC'C
Cuts at: 1366 1614 1366
Size: 248 3684
BbsI GAAGACnn'nnnn_
Cuts at: 301 301
Size: 3932
BciVI GTATCCnnnnn_n'
Cuts at: 476 1830 2591 3932 476
Size: 1354 761 1341 476
Fragments arranged by size:
1354 1341 761 476
BclI T'GATC_A
Cuts at: 681 1452 681
Size: 771 3161
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 430 896 1557 1650 2706 430
Size: 466 661 93 1056 1656
Fragments arranged by size:
1656 1056 661 466 93
BmrI ACTGGGnnnn_n'
Cuts at: 762 1144 1225 1560 3704 762
Size: 382 81 335 2144 990
Fragments arranged by size:
2144 990 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 565 2299 565
Size: 1734 2198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 338
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 1108 1987 2479 2777 216
Size: 892 879 492 298 1371
Fragments arranged by size:
1371 892 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 3932
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 1363 1611 2313 3809 1363
Size: 248 702 1496 1486
Fragments arranged by size:
1496 1486 702 248
BsaWI w'CCGG_w
Cuts at: 1121 1642 2320 2594 2741 3152 1121
Size: 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 236 1357 2115 2692 3188 236
Size: 1121 758 577 496 980
Fragments arranged by size:
1121 980 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 792 792
Size: 3932
BsiEI CG_ry'CG
Cuts at: 104 1474 1520 2728 104
Size: 1370 46 1208 1308
Fragments arranged by size:
1370 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 430 896 946 1724 1914 2706 430
Size: 466 50 778 190 792 1656
Fragments arranged by size:
1656 792 778 466 190 50
Bsp1286I G_dGCh'C
Cuts at: 430 896 946 1557 1650 1724 1914 1976 2706
Size: 466 50 611 93 74 190 62 730
Cuts at: 2706 430
Size: 1656
Fragments arranged by size:
1656 730 611 466 190 93 74 62
50
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 982 1196 1498 1879 982
Size: 214 302 381 3035
Fragments arranged by size:
3035 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 1930 2111 2320 1930
Size: 181 209 3542
Fragments arranged by size:
3542 209 181
BssSI C'ACGA_G
Cuts at: 559 2203 2561 559
Size: 1644 358 1930
Fragments arranged by size:
1930 1644 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1309
Size: 3932
BstBI TT'CG_AA
Cuts at: 292 2293 292
Size: 2001 1931
BstXI CCAn_nnnn'nTGG
Cuts at: 352 352
Size: 3932
BstYI r'GATC_y
Cuts at: 1 226 957 1424 1447 1782 2028 3040 3126
Size: 225 731 467 23 335 246 1012 86
Cuts at: 3126 3138 3484 1
Size: 12 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 225
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1882 2063 2226 1882
Size: 181 163 3588
Fragments arranged by size:
3588 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 1175
Size: 3932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 501 536 501
Size: 35 3897
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 849 1517 1691 2082 2109 3454 849
Size: 668 174 391 27 1345 1327
Fragments arranged by size:
1345 1327 668 391 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 255 772 1955 2165 255
Size: 517 1183 210 2022
Fragments arranged by size:
2022 1183 517 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2450 2596 3742 2140
Size: 310 146 1146 2330
Fragments arranged by size:
2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 944
Size: 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 338 432 516 600 684 769 1757 2189
Size: 129 94 84 84 84 85 988 432
Cuts at: 2189 2936 3204 209
Size: 747 268 937
Fragments arranged by size:
988 937 747 432 268 129 94 85
84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 1071 1071
Size: 3932
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
EcoRV GAT'ATC
Cuts at: 243 243
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 481 1951 481
Size: 1470 2462
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 1366 1614 2636 3615 1366
Size: 248 1022 979 1683
Fragments arranged by size:
1683 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 317 349 825 857 867 899 1774 1804 1806
Size: 32 476 32 10 32 875 30 2
Cuts at: 1806 1836 317
Size: 30 2413
Fragments arranged by size:
2413 875 476 32 32 32 30 30
10 2
HincII GTy'rAC
Cuts at: 538 971 538
Size: 433 3499
HindIII A'AGCT_T
Cuts at: 444 528 612 714 864 444
Size: 84 84 102 150 3512
Fragments arranged by size:
3512 150 102 84 84
KasI G'GCGC_C
Cuts at: 1362 1610 1362
Size: 248 3684
KpnI G_GTAC'C
Cuts at: 952 952
Size: 3932
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 145 2603 2787 145
Size: 2458 184 1290
Fragments arranged by size:
2458 1290 184
MscI TGG'CCA
Cuts at: 1693 1693
Size: 3932
MslI CAynn'nnrTG
Cuts at: 203 659 1921 2048 203
Size: 456 1262 127 2087
Fragments arranged by size:
2087 1262 456 127
MspA1I CmG'CkG
Cuts at: 1043 1357 1717 2730 2975 1043
Size: 314 360 1013 245 2000
Fragments arranged by size:
2000 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 1363 1611 1363
Size: 248 3684
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 985 1485 2392 985
Size: 500 907 2525
Fragments arranged by size:
2525 907 500
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PstI C_TGCA'G
Cuts at: 979 1664 979
Size: 685 3247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 1357 1717 1357
Size: 360 3572
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 946 946
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SfcI C'TryA_G
Cuts at: 33 221 301 672 975 1660 2653 2844 33
Size: 188 80 371 303 685 993 191 1121
Fragments arranged by size:
1121 993 685 371 303 191 188 80
SfoI GGC'GCC
Cuts at: 1364 1612 1364
Size: 248 3684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 954 954
Size: 3932
SmlI C'TyrA_G
Cuts at: 231 1087 2002 2494 2756 231
Size: 856 915 492 262 1407
Fragments arranged by size:
1407 915 856 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StuI AGG'CCT
Cuts at: 746 746
Size: 3932
StyI C'CwwG_G
Cuts at: 1066 1420 1066
Size: 354 3578
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 886 1515 1648 2107 2316 445
Size: 441 629 133 459 209 2061
Fragments arranged by size:
2061 629 459 441 209 133
TatI w'GTAC_w
Cuts at: 3447 3447
Size: 3932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1427 1427
Size: 3932
XmaI C'CCGG_G
Cuts at: 952 952
Size: 3932
XmnI GAAnn'nnTTC
Cuts at: 529 781 529
Size: 252 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI DraIII EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI