Restriction map for
pF1KB8925
(Circular) (Six-base) MAPSORT of: pF1KB8925.seq Check: 6478 from: 1 to: 3932
pF1KB8925 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 560 948 560
Size: 388 3544
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 340 1757 2189 2936 3204 310
Size: 30 1417 432 747 268 1038
Fragments arranged by size:
1417 1038 747 432 268 30
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 282 774 831 1237 2804 282
Size: 492 57 406 1567 1410
Fragments arranged by size:
1567 1410 492 406 57
ApaI G_GGCC'C
Cuts at: 224 224
Size: 3932
ApaLI G'TGCA_C
Cuts at: 2702 2702
Size: 3932
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 223 462 574 665 952 223
Size: 239 112 91 287 3203
Fragments arranged by size:
3203 287 239 112 91
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 259 387 560 570 948 1362 1610 1645 259
Size: 128 173 10 378 414 248 35 2546
Fragments arranged by size:
2546 414 378 248 173 128 35 10
BanII G_rGCy'C
Cuts at: 187 224 425 946 1976 187
Size: 37 201 521 1030 2143
Fragments arranged by size:
2143 1030 521 201 37
BbeI G_GCGC'C
Cuts at: 263 391 574 1366 1614 263
Size: 128 183 792 248 2581
Fragments arranged by size:
2581 792 248 183 128
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglI GCCn_nnn'nGGC
Cuts at: 238 252 238
Size: 14 3918
BglII A'GATC_T
Cuts at: 626 1447 3484 626
Size: 821 2037 1074
Fragments arranged by size:
2037 1074 821
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 224 725 1557 1650 2706 224
Size: 501 832 93 1056 1450
Fragments arranged by size:
1450 1056 832 501 93
BmrI ACTGGGnnnn_n'
Cuts at: 849 873 1144 1225 1560 3704 849
Size: 24 271 81 335 2144 1077
Fragments arranged by size:
2144 1077 335 271 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 2299 201
Size: 2098 1834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 1108 1987 2479 2777 387
Size: 721 879 492 298 1542
Fragments arranged by size:
1542 879 721 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 451 3636 451
Size: 3185 747
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 260 388 479 571 691 1363 1611 2313 3809
Size: 128 91 92 120 672 248 702 1496
Cuts at: 3809 260
Size: 383
Fragments arranged by size:
1496 702 672 383 248 128 120 92
91
BsaWI w'CCGG_w
Cuts at: 201 441 1121 1642 2320 2594 2741 3152 201
Size: 240 680 521 678 274 147 411 981
Fragments arranged by size:
981 680 678 521 411 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 391 421 391
Size: 30 3902
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 193
Size: 3932
BseYI C'CCAG_C
Cuts at: 249 911 1357 2115 2692 3188 249
Size: 662 446 758 577 496 993
Fragments arranged by size:
993 758 662 577 496 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 352 295
Size: 57 3875
BsiEI CG_ry'CG
Cuts at: 104 431 1474 1520 2728 104
Size: 327 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 327 46
BsiHKAI G_wGCw'C
Cuts at: 946 1724 1914 2706 946
Size: 778 190 792 2172
Fragments arranged by size:
2172 792 778 190
BsmI GAATG_Cn'
Cuts at: 800 800
Size: 3932
Bsp1286I G_dGCh'C
Cuts at: 187 224 425 725 946 1557 1650 1724 1914
Size: 37 201 300 221 611 93 74 190
Cuts at: 1914 1976 2706 187
Size: 62 730 1413
Fragments arranged by size:
1413 730 611 300 221 201 190 93
74 62 37
BspEI T'CCGG_A
Cuts at: 201 3152 201
Size: 2951 981
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 609 982 1196 1498 1879 167
Size: 442 373 214 302 381 2220
Fragments arranged by size:
2220 442 381 373 302 214
BsrBI CCG'CTC
Cuts at: 50 540 2224 2278 3762 3927 50
Size: 490 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 490 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 481 1930 2111 2320 481
Size: 1449 181 209 2093
Fragments arranged by size:
2093 1449 209 181
BssHII G'CGCG_C
Cuts at: 660 821 660
Size: 161 3771
BssSI C'ACGA_G
Cuts at: 2203 2561 2203
Size: 358 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 282 831 840 1309 282
Size: 549 9 469 2905
Fragments arranged by size:
2905 549 469 9
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstYI r'GATC_y
Cuts at: 1 626 957 1424 1447 1782 2028 3040 3126
Size: 625 331 467 23 335 246 1012 86
Cuts at: 3126 3138 3484 1
Size: 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 331 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 395 395
Size: 3932
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1882 2063 2226 1882
Size: 181 163 3588
Fragments arranged by size:
3588 181 163
BtsI GCAGTG_nn'
Cuts at: 922 1175 922
Size: 253 3679
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 191 338 428 590 716 866 1517 1691 2082
Size: 147 90 162 126 150 651 174 391
Cuts at: 2082 2109 3454 191
Size: 27 1345 669
Fragments arranged by size:
1345 669 651 391 174 162 150 147
126 90 27
EagI C'GGCC_G
Cuts at: 428 1517 428
Size: 1089 2843
EarI CTCTTCn'nnn_
Cuts at: 203 1955 2165 203
Size: 1752 210 1970
Fragments arranged by size:
1970 1752 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 2140 2450 2596 3742 592
Size: 1548 310 146 1146 782
Fragments arranged by size:
1548 1146 782 310 146
EcoICRI GAG'CTC
Cuts at: 944 944
Size: 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 340 556 1757 2189 2936 3204 310
Size: 30 216 1201 432 747 268 1038
Fragments arranged by size:
1201 1038 747 432 268 216 30
EcoNI CCTnn'n_nnAGG
Cuts at: 904 904
Size: 3932
EcoO109I rG'GnC_Cy
Cuts at: 220 351 771 1071 220
Size: 131 420 300 3081
Fragments arranged by size:
3081 420 300 131
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 168 315 1951 168
Size: 147 1636 2149
Fragments arranged by size:
2149 1636 147
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 263 391 546 574 1366 1614 2636 3615 263
Size: 128 155 28 792 248 1022 979 580
Fragments arranged by size:
1022 979 792 580 248 155 128 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 1774 1804 1806 1836 391
Size: 32 1351 30 2 30 2487
Fragments arranged by size:
2487 1351 32 30 30 2
HincII GTy'rAC
Cuts at: 971 971
Size: 3932
KasI G'GCGC_C
Cuts at: 259 387 570 1362 1610 259
Size: 128 183 792 248 2581
Fragments arranged by size:
2581 792 248 183 128
KpnI G_GTAC'C
Cuts at: 564 952 564
Size: 388 3544
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2603 2787 2603
Size: 184 3748
MscI TGG'CCA
Cuts at: 340 592 1693 340
Size: 252 1101 2579
Fragments arranged by size:
2579 1101 252
MslI CAynn'nnrTG
Cuts at: 1921 2048 1921
Size: 127 3805
MspA1I CmG'CkG
Cuts at: 249 558 1043 1357 1717 2730 2975 249
Size: 309 485 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 485 360 314 309 245
NaeI GCC'GGC
Cuts at: 483 2113 483
Size: 1630 2302
NarI GG'CG_CC
Cuts at: 260 388 571 1363 1611 260
Size: 128 183 792 248 2581
Fragments arranged by size:
2581 792 248 183 128
NgoMIV G'CCGG_C
Cuts at: 481 2111 481
Size: 1630 2302
NspI r_CATG'y
Cuts at: 843 985 1485 2392 843
Size: 142 500 907 2383
Fragments arranged by size:
2383 907 500 142
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PflMI CCAn_nnn'nTGG
Cuts at: 774 774
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PpuMI rG'GwC_Cy
Cuts at: 771 771
Size: 3932
PspOMI G'GGCC_C
Cuts at: 220 220
Size: 3932
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 286 318 631 663 286
Size: 32 313 32 3555
Fragments arranged by size:
3555 313 32 32
PstI C_TGCA'G
Cuts at: 836 979 1664 836
Size: 143 685 3104
Fragments arranged by size:
3104 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 1357 1717 1357
Size: 360 3572
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 946 946
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 361 3449 361
Size: 3088 844
SfcI C'TryA_G
Cuts at: 33 816 832 975 1660 2653 2844 33
Size: 783 16 143 685 993 191 1121
Fragments arranged by size:
1121 993 783 685 191 143 16
SfoI GGC'GCC
Cuts at: 261 389 572 1364 1612 261
Size: 128 183 792 248 2581
Fragments arranged by size:
2581 792 248 183 128
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 464 667 954 464
Size: 203 287 3442
Fragments arranged by size:
3442 287 203
SmlI C'TyrA_G
Cuts at: 402 1087 2002 2494 2756 402
Size: 685 915 492 262 1578
Fragments arranged by size:
1578 915 685 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
SrfI GCCC'GGGC
Cuts at: 667 667
Size: 3932
StyI C'CwwG_G
Cuts at: 515 1066 1420 515
Size: 551 354 3027
Fragments arranged by size:
3027 551 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1515 1648 2107 2316 129
Size: 1386 133 459 209 1745
Fragments arranged by size:
1745 1386 459 209 133
TatI w'GTAC_w
Cuts at: 359 845 857 3447 359
Size: 486 12 2590 844
Fragments arranged by size:
2590 844 486 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1427 1427
Size: 3932
XmaI C'CCGG_G
Cuts at: 462 665 952 462
Size: 203 287 3442
Fragments arranged by size:
3442 287 203
XmnI GAAnn'nnTTC
Cuts at: 298 298
Size: 3932
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmBI BsrGI BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI ZraI