Restriction map for
pF1KB8923
(Circular) (Six-base) MAPSORT of: pF1KB8923.seq Check: 1258 from: 1 to: 3923
pF1KB8923 3923 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 605 605
Size: 3923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 730 762 730
Size: 32 3891
AlwNI CAG_nnn'CTG
Cuts at: 729 1228 2795 729
Size: 499 1567 1857
Fragments arranged by size:
1857 1567 499
ApaI G_GGCC'C
Cuts at: 702 702
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 282 943 282
Size: 661 3262
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 345 487 612 939 1353 1601 1636 345
Size: 142 125 327 414 248 35 2632
Fragments arranged by size:
2632 414 327 248 142 125 35
BanII G_rGCy'C
Cuts at: 115 289 359 610 702 937 1967 115
Size: 174 70 251 92 235 1030 2071
Fragments arranged by size:
2071 1030 251 235 174 92 70
BbeI G_GCGC'C
Cuts at: 349 491 616 1357 1605 349
Size: 142 125 741 248 2667
Fragments arranged by size:
2667 741 248 142 125
BbvCI CC'TCA_GC
Cuts at: 395 395
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 284 299 343 455 689 706 284
Size: 15 44 112 234 17 3501
Fragments arranged by size:
3501 234 112 44 17 15
BglII A'GATC_T
Cuts at: 632 1438 3475 632
Size: 806 2037 1080
Fragments arranged by size:
2037 1080 806
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 702 1548 1641 2697 702
Size: 846 93 1056 1928
Fragments arranged by size:
1928 1056 846 93
BmgBI CAC'GTC
Cuts at: 380 380
Size: 3923
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 751 757 465
Size: 286 6 3631
Fragments arranged by size:
3631 286 6
Bpu10I CC'TnA_GC
Cuts at: 395 472 395
Size: 77 3846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 346 488 500 544 563 613 1354 1602 2304
Size: 142 12 44 19 50 741 248 702
Cuts at: 2304 3800 346
Size: 1496 469
Fragments arranged by size:
1496 741 702 469 248 142 50 44
19 12
BsaWI w'CCGG_w
Cuts at: 304 547 842 1112 1633 2311 2585 2732 3143
Size: 243 295 270 521 678 274 147 411
Cuts at: 3143 304
Size: 1084
Fragments arranged by size:
1084 678 521 411 295 274 270 243
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 375 405 730 760 286
Size: 30 59 30 325 30 3449
Fragments arranged by size:
3449 325 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 383 571 383
Size: 188 3735
BseYI C'CCAG_C
Cuts at: 1348 2106 2683 3179 1348
Size: 758 577 496 2092
Fragments arranged by size:
2092 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 640
Size: 3923
BsiEI CG_ry'CG
Cuts at: 104 141 279 497 1465 1511 2719 104
Size: 37 138 218 968 46 1208 1308
Fragments arranged by size:
1308 1208 968 218 138 46 37
BsiHKAI G_wGCw'C
Cuts at: 115 937 1715 1905 2697 115
Size: 822 778 190 792 1341
Fragments arranged by size:
1341 822 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 289 359 610 702 937 1548 1641 1715
Size: 174 70 251 92 235 611 93 74
Cuts at: 1715 1905 1967 2697 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 251 235 190 174 93
92 74 70 62
BspEI T'CCGG_A
Cuts at: 304 547 3143 304
Size: 243 2596 1084
Fragments arranged by size:
2596 1084 243
BspHI T'CATG_A
Cuts at: 666 3099 666
Size: 2433 1490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 973 1187 1489 1870 227
Size: 746 214 302 381 2280
Fragments arranged by size:
2280 746 381 302 214
BsrBI CCG'CTC
Cuts at: 50 281 2215 2269 3753 3918 50
Size: 231 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 372 502 1921 2102 2311 372
Size: 130 1419 181 209 1984
Fragments arranged by size:
1984 1419 209 181 130
BssHII G'CGCG_C
Cuts at: 312 312
Size: 3923
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstYI r'GATC_y
Cuts at: 1 264 632 948 1415 1438 1773 2019 3031
Size: 263 368 316 467 23 335 246 1012
Cuts at: 3031 3117 3129 3475 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 368 346 335 316 263
246 86 23 12
BtgI C'CryG_G
Cuts at: 422 453 893 422
Size: 31 440 3452
Fragments arranged by size:
3452 440 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 276 291 374 494 890 1508 1682 2073 2100
Size: 15 83 120 396 618 174 391 27
Cuts at: 2100 3445 276
Size: 1345 754
Fragments arranged by size:
1345 754 618 396 391 174 120 83
27 15
EagI C'GGCC_G
Cuts at: 276 494 1508 276
Size: 218 1014 2691
Fragments arranged by size:
2691 1014 218
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 408 2131 2441 2587 3733 408
Size: 1723 310 146 1146 598
Fragments arranged by size:
1723 1146 598 310 146
EcoICRI GAG'CTC
Cuts at: 113 935 113
Size: 822 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 751 757 1748 2180 2927 3195 465
Size: 286 6 991 432 747 268 1193
Fragments arranged by size:
1193 991 747 432 286 268 6
EcoO109I rG'GnC_Cy
Cuts at: 698 905 1062 698
Size: 207 157 3559
Fragments arranged by size:
3559 207 157
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 593 649 1704 593
Size: 56 1055 2812
Fragments arranged by size:
2812 1055 56
HaeII r_GCGC'y
Cuts at: 338 349 491 616 1357 1605 2627 3606 338
Size: 11 142 125 741 248 1022 979 655
Fragments arranged by size:
1022 979 741 655 248 142 125 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1795 1797 1827 1765
Size: 30 2 30 3861
Fragments arranged by size:
3861 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 345 487 612 1353 1601 345
Size: 142 125 741 248 2667
Fragments arranged by size:
2667 741 248 142 125
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 1684 1684
Size: 3923
MslI CAynn'nnrTG
Cuts at: 176 886 1912 2039 176
Size: 710 1026 127 2060
Fragments arranged by size:
2060 1026 710 127
MspA1I CmG'CkG
Cuts at: 408 485 895 1034 1348 1708 2721 2966 408
Size: 77 410 139 314 360 1013 245 1365
Fragments arranged by size:
1365 1013 410 360 314 245 139 77
NaeI GCC'GGC
Cuts at: 374 504 2104 374
Size: 130 1600 2193
Fragments arranged by size:
2193 1600 130
NarI GG'CG_CC
Cuts at: 346 488 613 1354 1602 346
Size: 142 125 741 248 2667
Fragments arranged by size:
2667 741 248 142 125
NgoMIV G'CCGG_C
Cuts at: 372 502 2102 372
Size: 130 1600 2193
Fragments arranged by size:
2193 1600 130
NspI r_CATG'y
Cuts at: 228 799 976 1476 2383 228
Size: 571 177 500 907 1768
Fragments arranged by size:
1768 907 571 500 177
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 729 729
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 730 762 3062 3094 730
Size: 32 2300 32 1559
Fragments arranged by size:
2300 1559 32 32
PspOMI G'GGCC_C
Cuts at: 698 698
Size: 3923
PstI C_TGCA'G
Cuts at: 970 1655 970
Size: 685 3238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 1348 1708 1348
Size: 360 3563
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 115 937 115
Size: 822 3101
SacII CC_GC'GG
Cuts at: 896 896
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 966 1651 2644 2835 33
Size: 933 685 993 191 1121
Fragments arranged by size:
1121 993 933 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 706 706
Size: 3923
SfoI GGC'GCC
Cuts at: 347 489 614 1355 1603 347
Size: 142 125 741 248 2667
Fragments arranged by size:
2667 741 248 142 125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 228 976 1476 228
Size: 748 500 2675
Fragments arranged by size:
2675 748 500
StyI C'CwwG_G
Cuts at: 1057 1411 1057
Size: 354 3569
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 526 1506 1639 2098 2307 526
Size: 980 133 459 209 2142
Fragments arranged by size:
2142 980 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 286 318 375 407 3440 3472 286
Size: 32 57 32 3033 32 737
Fragments arranged by size:
3033 737 57 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
XmnI GAAnn'nnTTC
Cuts at: 745 745
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI