Restriction map for
pF1KB8919
(Circular) (Six-base) MAPSORT of: pF1KB8919.seq Check: 3395 from: 1 to: 3905
pF1KB8919 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 512 943 512
Size: 431 3474
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1730 2162 2909 3177 857
Size: 873 432 747 268 1585
Fragments arranged by size:
1585 873 747 432 268
AfeI AGC'GCT
Cuts at: 580 580
Size: 3905
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 261 1210 2777 261
Size: 949 1567 1389
Fragments arranged by size:
1567 1389 949
ApaI G_GGCC'C
Cuts at: 332 332
Size: 3905
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 154 530 909 3258 3514 154
Size: 376 379 2349 256 545
Fragments arranged by size:
2349 545 379 376 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 379 582 825 925 379
Size: 203 243 100 3359
Fragments arranged by size:
3359 243 203 100
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 408 421 921 1335 1583 1618 408
Size: 13 500 414 248 35 2695
Fragments arranged by size:
2695 500 414 248 35 13
BanII G_rGCy'C
Cuts at: 121 332 919 1949 121
Size: 211 587 1030 2077
Fragments arranged by size:
2077 1030 587 211
BbeI G_GCGC'C
Cuts at: 412 425 1339 1587 412
Size: 13 914 248 2730
Fragments arranged by size:
2730 914 248 13
BbsI GAAGACnn'nnnn_
Cuts at: 845 845
Size: 3905
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 110 1425 110
Size: 1315 2590
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 332 1530 1623 2679 332
Size: 1198 93 1056 1558
Fragments arranged by size:
1558 1198 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 468 307
Size: 161 3744
Bpu10I CC'TnA_GC
Cuts at: 296 296
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 670 709 1419 3456 6
Size: 664 39 710 2037 455
Fragments arranged by size:
2037 710 664 455 39
BsaHI Gr'CG_yC
Cuts at: 409 422 1336 1584 2286 3782 409
Size: 13 914 248 702 1496 532
Fragments arranged by size:
1496 914 702 532 248 13
BsaWI w'CCGG_w
Cuts at: 207 1094 1615 2293 2567 2714 3125 207
Size: 887 521 678 274 147 411 987
Fragments arranged by size:
987 887 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 466
Size: 30 3875
BseYI C'CCAG_C
Cuts at: 441 802 1330 2088 2665 3161 441
Size: 361 528 758 577 496 1185
Fragments arranged by size:
1185 758 577 528 496 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 297
Size: 3905
BsiEI CG_ry'CG
Cuts at: 104 222 791 1447 1493 2701 104
Size: 118 569 656 46 1208 1308
Fragments arranged by size:
1308 1208 656 569 118 46
BsiHKAI G_wGCw'C
Cuts at: 121 919 1697 1887 2679 121
Size: 798 778 190 792 1347
Fragments arranged by size:
1347 798 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 121 332 919 1530 1623 1697 1887 1949 2679
Size: 211 587 611 93 74 190 62 730
Cuts at: 2679 121
Size: 1347
Fragments arranged by size:
1347 730 611 587 211 190 93 74
62
BspEI T'CCGG_A
Cuts at: 207 3125 207
Size: 2918 987
BspHI T'CATG_A
Cuts at: 113 3081 113
Size: 2968 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 354 405 790 1903 2084 2293 354
Size: 51 385 1113 181 209 1966
Fragments arranged by size:
1966 1113 385 209 181 51
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111
Size: 929 467 23 335 246 1012 86 12
Cuts at: 3111 3457 1
Size: 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 348 517 348
Size: 169 3736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
ClaI AT'CG_AT
Cuts at: 146 146
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 219 788 1490 1664 2055 2082 3427 219
Size: 569 702 174 391 27 1345 697
Fragments arranged by size:
1345 702 697 569 391 174 27
EagI C'GGCC_G
Cuts at: 219 788 1490 219
Size: 569 702 2634
Fragments arranged by size:
2634 702 569
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 417 2113 2423 2569 3715 417
Size: 1696 310 146 1146 607
Fragments arranged by size:
1696 1146 607 310 146
EcoICRI GAG'CTC
Cuts at: 119 917 119
Size: 798 3107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 468 857 1730 2162 2909 3177 307
Size: 161 389 873 432 747 268 1035
Fragments arranged by size:
1035 873 747 432 389 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 416 416
Size: 3905
EcoO109I rG'GnC_Cy
Cuts at: 328 1044 328
Size: 716 3189
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FseI GG_CCGG'CC
Cuts at: 358 358
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 303 412 425 491 582 797 857 1339 1587
Size: 109 13 66 91 215 60 482 248
Cuts at: 1587 2609 3588 303
Size: 1022 979 620
Fragments arranged by size:
1022 979 620 482 248 215 109 91
66 60 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1747 1777 1779 1809 466
Size: 32 1249 30 2 30 2562
Fragments arranged by size:
2562 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 408 421 1335 1583 408
Size: 13 914 248 2730
Fragments arranged by size:
2730 914 248 13
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 112 1894 2021 112
Size: 1782 127 1996
Fragments arranged by size:
1996 1782 127
MspA1I CmG'CkG
Cuts at: 1016 1330 1690 2703 2948 1016
Size: 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 360 314 245
NaeI GCC'GGC
Cuts at: 356 407 792 2086 356
Size: 51 385 1294 2175
Fragments arranged by size:
2175 1294 385 51
NarI GG'CG_CC
Cuts at: 409 422 1336 1584 409
Size: 13 914 248 2730
Fragments arranged by size:
2730 914 248 13
NcoI C'CATG_G
Cuts at: 517 517
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 354 405 790 2084 354
Size: 51 385 1294 2175
Fragments arranged by size:
2175 1294 385 51
NruI TCG'CGA
Cuts at: 342 342
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PfoI T'CCnGG_A
Cuts at: 611 611
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PsiI TTA'TAA
Cuts at: 560 560
Size: 3905
PspOMI G'GGCC_C
Cuts at: 328 328
Size: 3905
PspXI vC'TCGA_Gb
Cuts at: 582 582
Size: 3905
PstI C_TGCA'G
Cuts at: 460 952 1637 460
Size: 492 685 2728
Fragments arranged by size:
2728 685 492
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 121 919 121
Size: 798 3107
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SexAI A'CCwGG_T
Cuts at: 892 892
Size: 3905
SfcI C'TryA_G
Cuts at: 33 456 948 1633 2626 2817 33
Size: 423 492 685 993 191 1121
Fragments arranged by size:
1121 993 685 492 423 191
SfoI GGC'GCC
Cuts at: 410 423 1337 1585 410
Size: 13 914 248 2730
Fragments arranged by size:
2730 914 248 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 827 927 827
Size: 100 3805
SmlI C'TyrA_G
Cuts at: 582 1060 1975 2467 2729 582
Size: 478 915 492 262 1758
Fragments arranged by size:
1758 915 492 478 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 175 214 175
Size: 39 3866
StyI C'CwwG_G
Cuts at: 517 1039 1393 517
Size: 522 354 3029
Fragments arranged by size:
3029 522 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XhoI C'TCGA_G
Cuts at: 582 582
Size: 3905
XmaI C'CCGG_G
Cuts at: 825 925 825
Size: 100 3805
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PfoI PmeI PpiI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XmnI ZraI