Restriction map for
pF1KB8918
(Circular) (Six-base) MAPSORT of: pF1KB8918.seq Check: 9515 from: 1 to: 3899
pF1KB8918 3899 bp
With 182 enzymes: SgfI *
October 2, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 937 937
Size: 3899
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1724 2156 2903 3171 779
Size: 945 432 747 268 1507
Fragments arranged by size:
1507 945 747 432 268
AflIII A'CryG_T
Cuts at: 1251 2355 3332 1251
Size: 1104 977 1818
Fragments arranged by size:
1818 1104 977
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AlwNI CAG_nnn'CTG
Cuts at: 1204 2771 1204
Size: 1567 2332
ApaLI G'TGCA_C
Cuts at: 695 2669 695
Size: 1974 1925
ApoI r'AATT_y
Cuts at: 246 385 393 497 513 581 903 3252 3508
Size: 139 8 104 16 68 322 2349 256
Cuts at: 3508 246
Size: 637
Fragments arranged by size:
2349 637 322 256 139 104 68 16
8
AseI AT'TA_AT
Cuts at: 20 20
Size: 3899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 919 919
Size: 3899
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 915 1329 1577 1612 915
Size: 414 248 35 3202
Fragments arranged by size:
3202 414 248 35
BanII G_rGCy'C
Cuts at: 913 1943 913
Size: 1030 2869
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 1419 1419
Size: 3899
BfrBI ATG'CAT
Cuts at: 381 381
Size: 3899
BglII A'GATC_T
Cuts at: 1414 3451 1414
Size: 2037 1862
BlpI GC'TnA_GC
Cuts at: 1011 1011
Size: 3899
Bme1580I G_kGCm'C
Cuts at: 699 1524 1617 2673 699
Size: 825 93 1056 1925
Fragments arranged by size:
1925 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 1111 1192 1527 3671 1111
Size: 81 335 2144 1339
Fragments arranged by size:
2144 1339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 3899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 695 863 611
Size: 84 168 3647
Fragments arranged by size:
3647 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3603 3603
Size: 3899
BsaAI yAC'GTr
Cuts at: 1252 1252
Size: 3899
BsaBI GATnn'nnATC
Cuts at: 6 1413 3450 6
Size: 1407 2037 455
Fragments arranged by size:
2037 1407 455
BsaHI Gr'CG_yC
Cuts at: 1330 1578 2280 3776 1330
Size: 248 702 1496 1453
Fragments arranged by size:
1496 1453 702 248
BsaWI w'CCGG_w
Cuts at: 1088 1609 2287 2561 2708 3119 1088
Size: 521 678 274 147 411 1868
Fragments arranged by size:
1868 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1324 2082 2659 3155 1324
Size: 758 577 496 2068
Fragments arranged by size:
2068 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1441 1487 2695 104
Size: 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 699 913 1691 1881 2673 699
Size: 214 778 190 792 1925
Fragments arranged by size:
1925 792 778 214 190
Bsp1286I G_dGCh'C
Cuts at: 699 913 1524 1617 1691 1881 1943 2673 699
Size: 214 611 93 74 190 62 730 1925
Fragments arranged by size:
1925 730 611 214 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3119 3119
Size: 3899
BspHI T'CATG_A
Cuts at: 3075 3075
Size: 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1811 1811
Size: 3899
BsrFI r'CCGG_y
Cuts at: 1897 2078 2287 1897
Size: 181 209 3509
Fragments arranged by size:
3509 209 181
BssSI C'ACGA_G
Cuts at: 2170 2528 2170
Size: 358 3541
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1276
Size: 3899
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstYI r'GATC_y
Cuts at: 1 924 1391 1414 1749 1995 3007 3093 3105
Size: 923 467 23 335 246 1012 86 12
Cuts at: 3105 3451 1
Size: 346 449
Fragments arranged by size:
1012 923 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1849 2030 2193 1849
Size: 181 163 3555
Fragments arranged by size:
3555 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1142
Size: 3899
DraI TTT'AAA
Cuts at: 717 898 717
Size: 181 3718
DraIII CAC_nnn'GTG
Cuts at: 703 703
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1605 2463 1605
Size: 858 3041
EaeI y'GGCC_r
Cuts at: 1484 1658 2049 2076 3421 1484
Size: 174 391 27 1345 1962
Fragments arranged by size:
1962 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1484 1484
Size: 3899
EarI CTCTTCn'nnn_
Cuts at: 106 524 755 1922 2132 106
Size: 418 231 1167 210 1873
Fragments arranged by size:
1873 1167 418 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2107 2417 2563 3709 2107
Size: 310 146 1146 2297
Fragments arranged by size:
2297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 695 779 863 1724 2156 2903 3171 611
Size: 84 84 84 861 432 747 268 1339
Fragments arranged by size:
1339 861 747 432 268 84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 1038 1038
Size: 3899
EcoRI G'AATT_C
Cuts at: 903 903
Size: 3899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3899
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 1333 1581 2603 3582 1333
Size: 248 1022 979 1650
Fragments arranged by size:
1650 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1741 1771 1773 1803 1741
Size: 30 2 30 3837
Fragments arranged by size:
3837 30 30 2
HincII GTy'rAC
Cuts at: 938 938
Size: 3899
HindIII A'AGCT_T
Cuts at: 878 878
Size: 3899
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2754 2570
Size: 184 3715
MscI TGG'CCA
Cuts at: 1660 1660
Size: 3899
MslI CAynn'nnrTG
Cuts at: 858 1888 2015 858
Size: 1030 127 2742
Fragments arranged by size:
2742 1030 127
MspA1I CmG'CkG
Cuts at: 1010 1324 1684 2697 2942 1010
Size: 314 360 1013 245 1967
Fragments arranged by size:
1967 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 3899
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 3899
NsiI A_TGCA'T
Cuts at: 383 383
Size: 3899
NspI r_CATG'y
Cuts at: 471 952 1452 2359 471
Size: 481 500 907 2011
Fragments arranged by size:
2011 907 500 481
PciI A'CATG_T
Cuts at: 2355 2355
Size: 3899
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PsiI TTA'TAA
Cuts at: 308 308
Size: 3899
PstI C_TGCA'G
Cuts at: 946 1631 946
Size: 685 3214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 1324 1684 1324
Size: 360 3539
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 3416 3416
Size: 3899
SfcI C'TryA_G
Cuts at: 33 942 1627 2620 2811 33
Size: 909 685 993 191 1121
Fragments arranged by size:
1121 993 909 685 191
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 1054 1969 2461 2723 1054
Size: 915 492 262 2230
Fragments arranged by size:
2230 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 471 952 1452 471
Size: 481 500 2918
Fragments arranged by size:
2918 500 481
SspI AAT'ATT
Cuts at: 350 350
Size: 3899
StyI C'CwwG_G
Cuts at: 297 1033 1387 297
Size: 736 354 2809
Fragments arranged by size:
2809 736 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1482 1615 2074 2283 1482
Size: 133 459 209 3098
Fragments arranged by size:
3098 459 209 133
TatI w'GTAC_w
Cuts at: 3414 3414
Size: 3899
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 3867
Tth111I GACn'n_nGTC
Cuts at: 1696 1696
Size: 3899
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1394 1394
Size: 3899
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI