Restriction map for
pF1KB8917
(Circular) (Six-base) MAPSORT of: pF1KB8917.seq Check: 316 from: 1 to: 3893
pF1KB8917 3893 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 931 931
Size: 3893
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1718 2150 2897 3165 1718
Size: 432 747 268 2446
Fragments arranged by size:
2446 747 432 268
AfeI AGC'GCT
Cuts at: 694 694
Size: 3893
AflIII A'CryG_T
Cuts at: 530 1245 2349 3326 530
Size: 715 1104 977 1097
Fragments arranged by size:
1104 1097 977 715
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AlwNI CAG_nnn'CTG
Cuts at: 576 720 1198 2765 576
Size: 144 478 1567 1704
Fragments arranged by size:
1704 1567 478 144
ApaI G_GGCC'C
Cuts at: 125 125
Size: 3893
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 897 3246 3502 897
Size: 2349 256 1288
Fragments arranged by size:
2349 1288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 397 478 544 586 785 913 397
Size: 81 66 42 199 128 3377
Fragments arranged by size:
3377 199 128 81 66 42
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 237 306 367 412 482 909 1323 1571 1606
Size: 69 61 45 70 427 414 248 35
Cuts at: 1606 237
Size: 2524
Fragments arranged by size:
2524 427 414 248 70 69 61 45
35
BanII G_rGCy'C
Cuts at: 125 207 907 1937 125
Size: 82 700 1030 2081
Fragments arranged by size:
2081 1030 700 82
BbeI G_GCGC'C
Cuts at: 310 371 416 486 1327 1575 310
Size: 61 45 70 841 248 2628
Fragments arranged by size:
2628 841 248 70 61 45
BbsI GAAGACnn'nnnn_
Cuts at: 176 176
Size: 3893
BciVI GTATCCnnnnn_n'
Cuts at: 1791 2552 3893 1791
Size: 761 1341 1791
Fragments arranged by size:
1791 1341 761
BclI T'GATC_A
Cuts at: 1413 1413
Size: 3893
BglI GCCn_nnn'nGGC
Cuts at: 112 202 112
Size: 90 3803
BglII A'GATC_T
Cuts at: 1408 3445 1408
Size: 2037 1856
BlpI GC'TnA_GC
Cuts at: 1005 1005
Size: 3893
Bme1580I G_kGCm'C
Cuts at: 125 317 1518 1611 2667 125
Size: 192 1201 93 1056 1351
Fragments arranged by size:
1351 1201 1056 192 93
BmgBI CAC'GTC
Cuts at: 299 299
Size: 3893
BmrI ACTGGGnnnn_n'
Cuts at: 1105 1186 1521 3665 1105
Size: 81 335 2144 1333
Fragments arranged by size:
2144 1333 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 3893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1069 1948 2440 2738 445
Size: 624 879 492 298 1600
Fragments arranged by size:
1600 879 624 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 141 1246 141
Size: 1105 2788
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 307 368 413 470 483 1324 1572 2274 3770
Size: 61 45 57 13 841 248 702 1496
Cuts at: 3770 307
Size: 430
Fragments arranged by size:
1496 841 702 430 248 61 57 45
13
BsaWI w'CCGG_w
Cuts at: 1082 1603 2281 2555 2702 3113 1082
Size: 521 678 274 147 411 1862
Fragments arranged by size:
1862 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 661 691 721 751 661
Size: 30 30 30 3803
Fragments arranged by size:
3803 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 637 637
Size: 3893
BseYI C'CCAG_C
Cuts at: 226 353 427 643 1318 2076 2653 3149 226
Size: 127 74 216 675 758 577 496 970
Fragments arranged by size:
970 758 675 577 496 216 127 74
BsiEI CG_ry'CG
Cuts at: 104 440 561 1435 1481 2689 104
Size: 336 121 874 46 1208 1308
Fragments arranged by size:
1308 1208 874 336 121 46
BsiHKAI G_wGCw'C
Cuts at: 907 1685 1875 2667 907
Size: 778 190 792 2133
Fragments arranged by size:
2133 792 778 190
BsiWI C'GTAC_G
Cuts at: 344 344
Size: 3893
BsmBI CGTCTCn'nnnn_
Cuts at: 423 423
Size: 3893
Bsp1286I G_dGCh'C
Cuts at: 125 207 317 907 1518 1611 1685 1875 1937
Size: 82 110 590 611 93 74 190 62
Cuts at: 1937 2667 125
Size: 730 1351
Fragments arranged by size:
1351 730 611 590 190 110 93 82
74 62
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 3069 3069
Size: 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 801 943 1157 1459 1840 801
Size: 142 214 302 381 2854
Fragments arranged by size:
2854 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 677 807 2185 2239 3723 3888 50
Size: 627 130 1378 54 1484 165 55
Fragments arranged by size:
1484 1378 627 165 130 55 54
BsrDI GCAATG_nn'
Cuts at: 1805 1805
Size: 3893
BsrFI r'CCGG_y
Cuts at: 282 604 1891 2072 2281 282
Size: 322 1287 181 209 1894
Fragments arranged by size:
1894 1287 322 209 181
BssSI C'ACGA_G
Cuts at: 681 2164 2522 681
Size: 1483 358 2052
Fragments arranged by size:
2052 1483 358
BstAPI GCAn_nnn'nTGC
Cuts at: 576 1270 576
Size: 694 3199
BstBI TT'CG_AA
Cuts at: 2254 2254
Size: 3893
BstXI CCAn_nnnn'nTGG
Cuts at: 234 234
Size: 3893
BstYI r'GATC_y
Cuts at: 1 918 1385 1408 1743 1989 3001 3087 3099
Size: 917 467 23 335 246 1012 86 12
Cuts at: 3099 3445 1
Size: 346 449
Fragments arranged by size:
1012 917 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 329 653 329
Size: 324 3569
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1843 2024 2187 1843
Size: 181 163 3549
Fragments arranged by size:
3549 181 163
BtsI GCAGTG_nn'
Cuts at: 293 574 1136 293
Size: 281 562 3050
Fragments arranged by size:
3050 562 281
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DraIII CAC_nnn'GTG
Cuts at: 302 302
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 194 437 656 1478 1652 2043 2070 3415 194
Size: 243 219 822 174 391 27 1345 672
Fragments arranged by size:
1345 822 672 391 243 219 174 27
EagI C'GGCC_G
Cuts at: 437 1478 437
Size: 1041 2852
EarI CTCTTCn'nnn_
Cuts at: 748 1916 2126 748
Size: 1168 210 2515
Fragments arranged by size:
2515 1168 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 607 2101 2411 2557 3703 607
Size: 1494 310 146 1146 797
Fragments arranged by size:
1494 1146 797 310 146
EcoICRI GAG'CTC
Cuts at: 905 905
Size: 3893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1718 2150 2897 3165 748
Size: 970 432 747 268 1476
Fragments arranged by size:
1476 970 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 121 1032 121
Size: 911 2982
EcoRI G'AATT_C
Cuts at: 897 897
Size: 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1912 1912
Size: 3893
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 310 371 416 486 696 1327 1575 2597 3576
Size: 61 45 70 210 631 248 1022 979
Cuts at: 3576 310
Size: 627
Fragments arranged by size:
1022 979 631 627 248 210 70 61
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 391 423 1735 1765 1767 1797 244
Size: 32 115 32 1312 30 2 30 2340
Fragments arranged by size:
2340 1312 115 32 32 30 30 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 306 367 412 482 1323 1571 306
Size: 61 45 70 841 248 2628
Fragments arranged by size:
2628 841 248 70 61 45
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2564 2748 2564
Size: 184 3709
MscI TGG'CCA
Cuts at: 658 1654 658
Size: 996 2897
MslI CAynn'nnrTG
Cuts at: 164 1882 2009 164
Size: 1718 127 2048
Fragments arranged by size:
2048 1718 127
MspA1I CmG'CkG
Cuts at: 230 405 1004 1318 1678 2691 2936 230
Size: 175 599 314 360 1013 245 1187
Fragments arranged by size:
1187 1013 599 360 314 245 175
NaeI GCC'GGC
Cuts at: 284 606 2074 284
Size: 322 1468 2103
Fragments arranged by size:
2103 1468 322
NarI GG'CG_CC
Cuts at: 307 368 413 483 1324 1572 307
Size: 61 45 70 841 248 2628
Fragments arranged by size:
2628 841 248 70 61 45
NgoMIV G'CCGG_C
Cuts at: 282 604 2072 282
Size: 322 1468 2103
Fragments arranged by size:
2103 1468 322
NspI r_CATG'y
Cuts at: 387 534 946 1446 2353 387
Size: 147 412 500 907 1927
Fragments arranged by size:
1927 907 500 412 147
PasI CC'CwG_GG
Cuts at: 502 502
Size: 3893
PciI A'CATG_T
Cuts at: 530 2349 530
Size: 1819 2074
PflMI CCAn_nnn'nTGG
Cuts at: 720 720
Size: 3893
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3861
PspOMI G'GGCC_C
Cuts at: 121 121
Size: 3893
PspXI vC'TCGA_Gb
Cuts at: 397 397
Size: 3893
PstI C_TGCA'G
Cuts at: 940 1625 940
Size: 685 3208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 1318 1678 1318
Size: 360 3533
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 907 907
Size: 3893
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
SbfI CC_TGCA'GG
Cuts at: 940 940
Size: 3893
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SfcI C'TryA_G
Cuts at: 33 246 264 936 1621 2614 2805 33
Size: 213 18 672 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 213 191 18
SfoI GGC'GCC
Cuts at: 308 369 414 484 1325 1573 308
Size: 61 45 70 841 248 2628
Fragments arranged by size:
2628 841 248 70 61 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 480 546 787 915 480
Size: 66 241 128 3458
Fragments arranged by size:
3458 241 128 66
SmlI C'TyrA_G
Cuts at: 397 460 1048 1963 2455 2717 397
Size: 63 588 915 492 262 1573
Fragments arranged by size:
1573 915 588 492 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
StyI C'CwwG_G
Cuts at: 1027 1381 1027
Size: 354 3539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1476 1609 2068 2277 1476
Size: 133 459 209 3092
Fragments arranged by size:
3092 459 209 133
TatI w'GTAC_w
Cuts at: 3408 3408
Size: 3893
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 661 693 3410 3442 661
Size: 32 2717 32 1112
Fragments arranged by size:
2717 1112 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 924 924
Size: 3893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 1388 651
Size: 737 3156
XhoI C'TCGA_G
Cuts at: 397 397
Size: 3893
XmaI C'CCGG_G
Cuts at: 478 544 785 913 478
Size: 66 241 128 3458
Fragments arranged by size:
3458 241 128 66
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsgI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI