Restriction map for
pF1KB8914
(Circular) (Six-base) MAPSORT of: pF1KB8914.seq Check: 5407 from: 1 to: 3878
pF1KB8914 3878 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 506 916 506
Size: 410 3468
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1703 2135 2882 3150 1703
Size: 432 747 268 2431
Fragments arranged by size:
2431 747 432 268
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 556 2266 556
Size: 1710 2168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 3846
AlwNI CAG_nnn'CTG
Cuts at: 1183 2750 1183
Size: 1567 2311
ApaLI G'TGCA_C
Cuts at: 526 2648 526
Size: 2122 1756
ApoI r'AATT_y
Cuts at: 534 622 882 3231 3487 534
Size: 88 260 2349 256 925
Fragments arranged by size:
2349 925 260 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 252 307 437 475 898 252
Size: 55 130 38 423 3232
Fragments arranged by size:
3232 423 130 55 38
AvrII C'CTAG_G
Cuts at: 192 192
Size: 3878
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 205 408 420 433 650 894 1308 1556 1591
Size: 203 12 13 217 244 414 248 35
Cuts at: 1591 205
Size: 2492
Fragments arranged by size:
2492 414 248 244 217 203 35 13
12
BanII G_rGCy'C
Cuts at: 260 892 1922 260
Size: 632 1030 2216
Fragments arranged by size:
2216 1030 632
BbeI G_GCGC'C
Cuts at: 209 412 424 437 654 1312 1560 209
Size: 203 12 13 217 658 248 2527
Fragments arranged by size:
2527 658 248 217 203 13 12
BciVI GTATCCnnnnn_n'
Cuts at: 1776 2537 3878 1776
Size: 761 1341 1776
Fragments arranged by size:
1776 1341 761
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BglI GCCn_nnn'nGGC
Cuts at: 255 255
Size: 3878
BglII A'GATC_T
Cuts at: 704 1393 3430 704
Size: 689 2037 1152
Fragments arranged by size:
2037 1152 689
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 530 1503 1596 2652 530
Size: 973 93 1056 1756
Fragments arranged by size:
1756 1056 973 93
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1171 1506 3650 1090
Size: 81 335 2144 1318
Fragments arranged by size:
2144 1318 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 365
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1054 1933 2425 2723 472
Size: 582 879 492 298 1627
Fragments arranged by size:
1627 879 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 531 1231 531
Size: 700 3178
BsaBI GATnn'nnATC
Cuts at: 6 664 703 1392 3429 6
Size: 658 39 689 2037 455
Fragments arranged by size:
2037 689 658 455 39
BsaHI Gr'CG_yC
Cuts at: 206 409 421 434 651 1309 1557 2259 3755
Size: 203 12 13 217 658 248 702 1496
Cuts at: 3755 206
Size: 329
Fragments arranged by size:
1496 702 658 329 248 217 203 13
12
BsaWI w'CCGG_w
Cuts at: 556 719 1067 1588 2266 2540 2687 3098 556
Size: 163 348 521 678 274 147 411 1336
Fragments arranged by size:
1336 678 521 411 348 274 163 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 780 792 795 184
Size: 596 12 3 3267
Fragments arranged by size:
3267 596 12 3
BseYI C'CCAG_C
Cuts at: 396 817 1303 2061 2638 3134 396
Size: 421 486 758 577 496 1140
Fragments arranged by size:
1140 758 577 496 486 421
BsiEI CG_ry'CG
Cuts at: 104 500 1420 1466 2674 104
Size: 396 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 396 46
BsiHKAI G_wGCw'C
Cuts at: 530 892 1670 1860 2652 530
Size: 362 778 190 792 1756
Fragments arranged by size:
1756 792 778 362 190
Bsp1286I G_dGCh'C
Cuts at: 260 530 892 1503 1596 1670 1860 1922 2652
Size: 270 362 611 93 74 190 62 730
Cuts at: 2652 260
Size: 1486
Fragments arranged by size:
1486 730 611 362 270 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 113 3054 113
Size: 2941 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 928 1142 1444 1825 758
Size: 170 214 302 381 2811
Fragments arranged by size:
2811 381 302 214 170
BsrBI CCG'CTC
Cuts at: 50 379 2170 2224 3708 3873 50
Size: 329 1791 54 1484 165 55
Fragments arranged by size:
1791 1484 329 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 417 495 556 1876 2057 2266 417
Size: 78 61 1320 181 209 2029
Fragments arranged by size:
2029 1320 209 181 78 61
BssHII G'CGCG_C
Cuts at: 227 227
Size: 3878
BssSI C'ACGA_G
Cuts at: 2149 2507 2149
Size: 358 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 1255
Size: 3878
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstXI CCAn_nnnn'nTGG
Cuts at: 144 144
Size: 3878
BstYI r'GATC_y
Cuts at: 1 704 903 1370 1393 1728 1974 2986 3072
Size: 703 199 467 23 335 246 1012 86
Cuts at: 3072 3084 3430 1
Size: 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 246 199
86 23 12
BtgI C'CryG_G
Cuts at: 107 265 500 107
Size: 158 235 3485
Fragments arranged by size:
3485 235 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 1121 1121
Size: 3878
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DraIII CAC_nnn'GTG
Cuts at: 680 680
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 497 839 1463 1637 2028 2055 3400 497
Size: 342 624 174 391 27 1345 975
Fragments arranged by size:
1345 975 624 391 342 174 27
EagI C'GGCC_G
Cuts at: 497 1463 497
Size: 966 2912
EarI CTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 404 2086 2396 2542 3688 404
Size: 1682 310 146 1146 594
Fragments arranged by size:
1682 1146 594 310 146
EcoICRI GAG'CTC
Cuts at: 890 890
Size: 3878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 1703 2135 2882 3150 365
Size: 1338 432 747 268 1093
Fragments arranged by size:
1338 1093 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 293 293
Size: 3878
EcoO109I rG'GnC_Cy
Cuts at: 1017 1017
Size: 3878
EcoRI G'AATT_C
Cuts at: 534 882 534
Size: 348 3530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3878
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 209 325 412 424 437 654 696 1312 1560
Size: 116 87 12 13 217 42 616 248
Cuts at: 1560 2582 3561 209
Size: 1022 979 526
Fragments arranged by size:
1022 979 616 526 248 217 116 87
42 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1750 1752 1782 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 30 30 2
HincII GTy'rAC
Cuts at: 917 917
Size: 3878
KasI G'GCGC_C
Cuts at: 205 408 420 433 650 1308 1556 205
Size: 203 12 13 217 658 248 2527
Fragments arranged by size:
2527 658 248 217 203 13 12
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 637 668 2549 2733 479
Size: 158 31 1881 184 1624
Fragments arranged by size:
1881 1624 184 158 31
MscI TGG'CCA
Cuts at: 841 1639 841
Size: 798 3080
MslI CAynn'nnrTG
Cuts at: 112 1867 1994 112
Size: 1755 127 1996
Fragments arranged by size:
1996 1755 127
MspA1I CmG'CkG
Cuts at: 302 989 1303 1663 2676 2921 302
Size: 687 314 360 1013 245 1259
Fragments arranged by size:
1259 1013 687 360 314 245
NaeI GCC'GGC
Cuts at: 419 497 2059 419
Size: 78 1562 2238
Fragments arranged by size:
2238 1562 78
NarI GG'CG_CC
Cuts at: 206 409 421 434 651 1309 1557 206
Size: 203 12 13 217 658 248 2527
Fragments arranged by size:
2527 658 248 217 203 13 12
NcoI C'CATG_G
Cuts at: 107 107
Size: 3878
NgoMIV G'CCGG_C
Cuts at: 417 495 2057 417
Size: 78 1562 2238
Fragments arranged by size:
2238 1562 78
NspI r_CATG'y
Cuts at: 931 1431 2338 931
Size: 500 907 2471
Fragments arranged by size:
2471 907 500
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PfoI T'CCnGG_A
Cuts at: 381 381
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PmlI CAC'GTG
Cuts at: 531 531
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3846
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 535 567 535
Size: 32 3846
PstI C_TGCA'G
Cuts at: 925 1610 925
Size: 685 3193
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 1303 1663 1303
Size: 360 3518
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 892 892
Size: 3878
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 3395 3395
Size: 3878
SfcI C'TryA_G
Cuts at: 33 444 921 1606 2599 2790 33
Size: 411 477 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 411 191
SfoI GGC'GCC
Cuts at: 207 410 422 435 652 1310 1558 207
Size: 203 12 13 217 658 248 2527
Fragments arranged by size:
2527 658 248 217 203 13 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SgrAI Cr'CCGG_yG
Cuts at: 417 556 417
Size: 139 3739
SmaI CCC'GGG
Cuts at: 254 309 439 477 900 254
Size: 55 130 38 423 3232
Fragments arranged by size:
3232 423 130 55 38
SmlI C'TyrA_G
Cuts at: 487 1033 1948 2440 2702 487
Size: 546 915 492 262 1663
Fragments arranged by size:
1663 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 931 1431 931
Size: 500 3378
SrfI GCCC'GGGC
Cuts at: 439 439
Size: 3878
StyI C'CwwG_G
Cuts at: 107 192 1012 1366 107
Size: 85 820 354 2619
Fragments arranged by size:
2619 820 354 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1594 2053 2262 1461
Size: 133 459 209 3077
Fragments arranged by size:
3077 459 209 133
TatI w'GTAC_w
Cuts at: 3393 3393
Size: 3878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 252 307 437 475 898 252
Size: 55 130 38 423 3232
Fragments arranged by size:
3232 423 130 55 38
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SnaBI SspI StuI SwaI XhoI
XmnI ZraI