Restriction map for
pF1KB8911
(Circular) (Six-base) MAPSORT of: pF1KB8911.seq Check: 4301 from: 1 to: 3863
pF1KB8911 3863 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 143 338 901 143
Size: 195 563 3105
Fragments arranged by size:
3105 563 195
Acc65I G'GTAC_C
Cuts at: 386 879 386
Size: 493 3370
AclI AA'CG_TT
Cuts at: 3703 3703
Size: 3863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2120 2867 3135 1688
Size: 432 747 268 2416
Fragments arranged by size:
2416 747 432 268
AfeI AGC'GCT
Cuts at: 673 673
Size: 3863
AflIII A'CryG_T
Cuts at: 314 1215 2319 3296 314
Size: 901 1104 977 881
Fragments arranged by size:
1104 977 901 881
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AleI CACnn'nnGTG
Cuts at: 164 164
Size: 3863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 706 738 706
Size: 32 3831
AlwNI CAG_nnn'CTG
Cuts at: 699 1168 2735 699
Size: 469 1567 1827
Fragments arranged by size:
1827 1567 469
ApaLI G'TGCA_C
Cuts at: 2633 2633
Size: 3863
ApoI r'AATT_y
Cuts at: 584 867 3216 3472 584
Size: 283 2349 256 975
Fragments arranged by size:
2349 975 283 256
AscI GG'CGCG_CC
Cuts at: 476 476
Size: 3863
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 432 508 883 432
Size: 76 375 3412
Fragments arranged by size:
3412 375 76
BamHI G'GATC_C
Cuts at: 888 888
Size: 3863
BanI G'GyrC_C
Cuts at: 286 297 386 411 879 1293 1541 1576 286
Size: 11 89 25 468 414 248 35 2573
Fragments arranged by size:
2573 468 414 248 89 35 25 11
BanII G_rGCy'C
Cuts at: 214 310 877 1907 214
Size: 96 567 1030 2170
Fragments arranged by size:
2170 1030 567 96
BbeI G_GCGC'C
Cuts at: 290 301 415 1297 1545 290
Size: 11 114 882 248 2608
Fragments arranged by size:
2608 882 248 114 11
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2522 3863 1761
Size: 761 1341 1761
Fragments arranged by size:
1761 1341 761
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BglI GCCn_nnn'nGGC
Cuts at: 295 295
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 127 304 975 127
Size: 177 671 3015
Fragments arranged by size:
3015 671 177
Bme1580I G_kGCm'C
Cuts at: 505 1488 1581 2637 505
Size: 983 93 1056 1731
Fragments arranged by size:
1731 1056 983 93
BmrI ACTGGGnnnn_n'
Cuts at: 1075 1156 1491 3635 1075
Size: 81 335 2144 1303
Fragments arranged by size:
2144 1303 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 267 2230 267
Size: 1963 1900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 262 424 721 164
Size: 98 162 297 3306
Fragments arranged by size:
3306 297 162 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1918 2410 2708 1039
Size: 879 492 298 2194
Fragments arranged by size:
2194 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 287 298 365 412 1294 1542 2244 3740 287
Size: 11 67 47 882 248 702 1496 410
Fragments arranged by size:
1496 882 702 410 248 67 47 11
BsaWI w'CCGG_w
Cuts at: 1052 1573 2251 2525 2672 3083 1052
Size: 521 678 274 147 411 1832
Fragments arranged by size:
1832 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 706 736 706
Size: 30 3833
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 760 760
Size: 3863
BseYI C'CCAG_C
Cuts at: 471 1288 2046 2623 3119 471
Size: 817 758 577 496 1215
Fragments arranged by size:
1215 817 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 631 276
Size: 355 3508
BsiEI CG_ry'CG
Cuts at: 104 377 1405 1451 2659 104
Size: 273 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 273 46
BsiHKAI G_wGCw'C
Cuts at: 214 239 877 1655 1845 2637 214
Size: 25 638 778 190 792 1440
Fragments arranged by size:
1440 792 778 638 190 25
Bsp1286I G_dGCh'C
Cuts at: 214 239 310 505 877 1488 1581 1655 1845
Size: 25 71 195 372 611 93 74 190
Cuts at: 1845 1907 2637 214
Size: 62 730 1440
Fragments arranged by size:
1440 730 611 372 195 190 93 74
71 62 25
BspEI T'CCGG_A
Cuts at: 3083 3083
Size: 3863
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 913 1127 1429 1810 374
Size: 539 214 302 381 2427
Fragments arranged by size:
2427 539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2155 2209 3693 3858 50
Size: 2105 54 1484 165 55
Fragments arranged by size:
2105 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 1775
Size: 3863
BsrFI r'CCGG_y
Cuts at: 276 372 408 812 1861 2042 2251 276
Size: 96 36 404 1049 181 209 1888
Fragments arranged by size:
1888 1049 404 209 181 96 36
BssHII G'CGCG_C
Cuts at: 476 476
Size: 3863
BssSI C'ACGA_G
Cuts at: 160 252 2134 2492 160
Size: 92 1882 358 1531
Fragments arranged by size:
1882 1531 358 92
BstAPI GCAn_nnn'nTGC
Cuts at: 1240 1240
Size: 3863
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstYI r'GATC_y
Cuts at: 1 888 1355 1378 1713 1959 2971 3057 3069
Size: 887 467 23 335 246 1012 86 12
Cuts at: 3069 3415 1
Size: 346 449
Fragments arranged by size:
1012 887 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3519
Fragments arranged by size:
3519 181 163
BtsI GCAGTG_nn'
Cuts at: 1106 1106
Size: 3863
DraI TTT'AAA
Cuts at: 862 862
Size: 3863
DraIII CAC_nnn'GTG
Cuts at: 759 759
Size: 3863
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 198 374 761 1448 1622 2013 2040 3385 198
Size: 176 387 687 174 391 27 1345 676
Fragments arranged by size:
1345 687 676 391 387 176 174 27
EagI C'GGCC_G
Cuts at: 374 1448 374
Size: 1074 2789
EarI CTCTTCn'nnn_
Cuts at: 663 1886 2096 663
Size: 1223 210 2430
Fragments arranged by size:
2430 1223 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 624 831 2071 2381 2527 3673 475
Size: 149 207 1240 310 146 1146 665
Fragments arranged by size:
1240 1146 665 310 207 149 146
EcoICRI GAG'CTC
Cuts at: 212 875 212
Size: 663 3200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 262 424 721 1688 2120 2867 3135 164
Size: 98 162 297 967 432 747 268 892
Fragments arranged by size:
967 892 747 432 297 268 162 98
EcoO109I rG'GnC_Cy
Cuts at: 1002 1002
Size: 3863
EcoRI G'AATT_C
Cuts at: 867 867
Size: 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 277 290 301 415 675 1297 1545 2567 3546
Size: 13 11 114 260 622 248 1022 979
Cuts at: 3546 277
Size: 594
Fragments arranged by size:
1022 979 622 594 260 248 114 13
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 141 173 603 635 1705 1735 1737 1767 141
Size: 32 430 32 1070 30 2 30 2237
Fragments arranged by size:
2237 1070 430 32 32 30 30 2
HincII GTy'rAC
Cuts at: 144 902 144
Size: 758 3105
KasI G'GCGC_C
Cuts at: 286 297 411 1293 1541 286
Size: 11 114 882 248 2608
Fragments arranged by size:
2608 882 248 114 11
KpnI G_GTAC'C
Cuts at: 390 883 390
Size: 493 3370
MluI A'CGCG_T
Cuts at: 314 3296 314
Size: 2982 881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2718 2534
Size: 184 3679
MscI TGG'CCA
Cuts at: 200 763 1624 200
Size: 563 861 2439
Fragments arranged by size:
2439 861 563
MslI CAynn'nnrTG
Cuts at: 164 1852 1979 164
Size: 1688 127 2048
Fragments arranged by size:
2048 1688 127
MspA1I CmG'CkG
Cuts at: 303 468 974 1288 1648 2661 2906 303
Size: 165 506 314 360 1013 245 1260
Fragments arranged by size:
1260 1013 506 360 314 245 165
NaeI GCC'GGC
Cuts at: 374 410 2044 374
Size: 36 1634 2193
Fragments arranged by size:
2193 1634 36
NarI GG'CG_CC
Cuts at: 287 298 412 1294 1542 287
Size: 11 114 882 248 2608
Fragments arranged by size:
2608 882 248 114 11
NgoMIV G'CCGG_C
Cuts at: 372 408 2042 372
Size: 36 1634 2193
Fragments arranged by size:
2193 1634 36
NspI r_CATG'y
Cuts at: 916 1416 2323 916
Size: 500 907 2456
Fragments arranged by size:
2456 907 500
PasI CC'CwG_GG
Cuts at: 360 360
Size: 3863
PciI A'CATG_T
Cuts at: 2319 2319
Size: 3863
PflMI CCAn_nnn'nTGG
Cuts at: 699 699
Size: 3863
PfoI T'CCnGG_A
Cuts at: 319 661 319
Size: 342 3521
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 706 738 3002 3034 706
Size: 32 2264 32 1535
Fragments arranged by size:
2264 1535 32 32
PstI C_TGCA'G
Cuts at: 910 1595 910
Size: 685 3178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 1288 1648 1288
Size: 360 3503
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 214 877 214
Size: 663 3200
SalI G'TCGA_C
Cuts at: 142 900 142
Size: 758 3105
SapI GCTCTTCn'nnn_
Cuts at: 663 1886 2096 663
Size: 1223 210 2430
Fragments arranged by size:
2430 1223 210
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 3380 3380
Size: 3863
SfcI C'TryA_G
Cuts at: 33 906 1591 2584 2775 33
Size: 873 685 993 191 1121
Fragments arranged by size:
1121 993 873 685 191
SfoI GGC'GCC
Cuts at: 288 299 413 1295 1543 288
Size: 11 114 882 248 2608
Fragments arranged by size:
2608 882 248 114 11
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SgrAI Cr'CCGG_yG
Cuts at: 276 276
Size: 3863
SmaI CCC'GGG
Cuts at: 434 885 434
Size: 451 3412
SmlI C'TyrA_G
Cuts at: 1018 1933 2425 2687 1018
Size: 915 492 262 2194
Fragments arranged by size:
2194 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 916 1416 916
Size: 500 3363
StyI C'CwwG_G
Cuts at: 997 1351 997
Size: 354 3509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1579 2038 2247 1446
Size: 133 459 209 3062
Fragments arranged by size:
3062 459 209 133
TatI w'GTAC_w
Cuts at: 512 3378 512
Size: 2866 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1358 1358
Size: 3863
XmaI C'CCGG_G
Cuts at: 432 883 432
Size: 451 3412
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI