Restriction map for
pF1KB8908
(Circular) (Six-base) MAPSORT of: pF1KB8908.seq Check: 4151 from: 1 to: 3839
pF1KB8908 3839 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 877 877
Size: 3839
Acc65I G'GTAC_C
Cuts at: 855 855
Size: 3839
AclI AA'CG_TT
Cuts at: 3679 3679
Size: 3839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1664 2096 2843 3111 1664
Size: 432 747 268 2392
Fragments arranged by size:
2392 747 432 268
AflIII A'CryG_T
Cuts at: 1191 2295 3272 1191
Size: 1104 977 1758
Fragments arranged by size:
1758 1104 977
AgeI A'CCGG_T
Cuts at: 2227 2227
Size: 3839
AlwNI CAG_nnn'CTG
Cuts at: 1144 2711 1144
Size: 1567 2272
ApaI G_GGCC'C
Cuts at: 233 233
Size: 3839
ApaLI G'TGCA_C
Cuts at: 2609 2609
Size: 3839
ApoI r'AATT_y
Cuts at: 367 843 3192 3448 367
Size: 476 2349 256 758
Fragments arranged by size:
2349 758 476 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
AvaI C'yCGr_G
Cuts at: 755 859 755
Size: 104 3735
BamHI G'GATC_C
Cuts at: 864 864
Size: 3839
BanI G'GyrC_C
Cuts at: 243 295 855 1269 1517 1552 243
Size: 52 560 414 248 35 2530
Fragments arranged by size:
2530 560 414 248 52 35
BanII G_rGCy'C
Cuts at: 115 233 414 756 853 1883 115
Size: 118 181 342 97 1030 2071
Fragments arranged by size:
2071 1030 342 181 118 97
BbeI G_GCGC'C
Cuts at: 247 1273 1521 247
Size: 1026 248 2565
Fragments arranged by size:
2565 1026 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 414 448 414
Size: 34 3805
BciVI GTATCCnnnnn_n'
Cuts at: 1737 2498 3839 1737
Size: 761 1341 1737
Fragments arranged by size:
1737 1341 761
BclI T'GATC_A
Cuts at: 1359 1359
Size: 3839
BglII A'GATC_T
Cuts at: 689 1354 3391 689
Size: 665 2037 1137
Fragments arranged by size:
2037 1137 665
BlpI GC'TnA_GC
Cuts at: 343 413 951 343
Size: 70 538 3231
Fragments arranged by size:
3231 538 70
Bme1580I G_kGCm'C
Cuts at: 233 1464 1557 2613 233
Size: 1231 93 1056 1459
Fragments arranged by size:
1459 1231 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1051 1132 1467 3611 1051
Size: 81 335 2144 1279
Fragments arranged by size:
2144 1279 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 3839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1894 2386 2684 1015
Size: 879 492 298 2170
Fragments arranged by size:
2170 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 528 3543 528
Size: 3015 824
BsaAI yAC'GTr
Cuts at: 1192 1192
Size: 3839
BsaBI GATnn'nnATC
Cuts at: 6 1353 3390 6
Size: 1347 2037 455
Fragments arranged by size:
2037 1347 455
BsaHI Gr'CG_yC
Cuts at: 244 359 1270 1518 2220 3716 244
Size: 115 911 248 702 1496 367
Fragments arranged by size:
1496 911 702 367 248 115
BsaWI w'CCGG_w
Cuts at: 1028 1549 2227 2501 2648 3059 1028
Size: 521 678 274 147 411 1808
Fragments arranged by size:
1808 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 543 573 283
Size: 30 230 30 3549
Fragments arranged by size:
3549 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 778 811 823 778
Size: 33 12 3794
Fragments arranged by size:
3794 33 12
BseYI C'CCAG_C
Cuts at: 511 1264 2022 2599 3095 511
Size: 753 758 577 496 1255
Fragments arranged by size:
1255 758 753 577 496
BsiEI CG_ry'CG
Cuts at: 104 1381 1427 2635 104
Size: 1277 46 1208 1308
Fragments arranged by size:
1308 1277 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 340 853 1631 1821 2613 115
Size: 225 513 778 190 792 1341
Fragments arranged by size:
1341 792 778 513 225 190
BsmBI CGTCTCn'nnnn_
Cuts at: 350 512 350
Size: 162 3677
Bsp1286I G_dGCh'C
Cuts at: 115 233 340 414 756 853 1464 1557 1631
Size: 118 107 74 342 97 611 93 74
Cuts at: 1631 1821 1883 2613 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 342 190 118 107 97
93 74 74 62
BspEI T'CCGG_A
Cuts at: 3059 3059
Size: 3839
BspHI T'CATG_A
Cuts at: 3015 3015
Size: 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 889 1103 1405 1786 889
Size: 214 302 381 2942
Fragments arranged by size:
2942 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2131 2185 3669 3834 50
Size: 2081 54 1484 165 55
Fragments arranged by size:
2081 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1751 1751
Size: 3839
BsrFI r'CCGG_y
Cuts at: 151 168 185 578 1837 2018 2227 151
Size: 17 17 393 1259 181 209 1763
Fragments arranged by size:
1763 1259 393 209 181 17 17
BssSI C'ACGA_G
Cuts at: 2110 2468 2110
Size: 358 3481
BstAPI GCAn_nnn'nTGC
Cuts at: 1216 1216
Size: 3839
BstBI TT'CG_AA
Cuts at: 2200 2200
Size: 3839
BstYI r'GATC_y
Cuts at: 1 689 864 1331 1354 1689 1935 2947 3033
Size: 688 175 467 23 335 246 1012 86
Cuts at: 3033 3045 3391 1
Size: 12 346 449
Fragments arranged by size:
1012 688 467 449 346 335 246 175
86 23 12
BtgI C'CryG_G
Cuts at: 291 351 526 291
Size: 60 175 3604
Fragments arranged by size:
3604 175 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1789 1970 2133 1789
Size: 181 163 3495
Fragments arranged by size:
3495 181 163
BtsI GCAGTG_nn'
Cuts at: 564 1082 564
Size: 518 3321
DraI TTT'AAA
Cuts at: 838 838
Size: 3839
DrdI GACnn_nn'nnGTC
Cuts at: 1545 2403 1545
Size: 858 2981
EaeI y'GGCC_r
Cuts at: 1424 1598 1989 2016 3361 1424
Size: 174 391 27 1345 1902
Fragments arranged by size:
1902 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1424 1424
Size: 3839
EarI CTCTTCn'nnn_
Cuts at: 788 800 1862 2072 788
Size: 12 1062 210 2555
Fragments arranged by size:
2555 1062 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 447 2047 2357 2503 3649 447
Size: 1600 310 146 1146 637
Fragments arranged by size:
1600 1146 637 310 146
EcoICRI GAG'CTC
Cuts at: 113 851 113
Size: 738 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1664 2096 2843 3111 1664
Size: 432 747 268 2392
Fragments arranged by size:
2392 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 978 978
Size: 3839
EcoRI G'AATT_C
Cuts at: 843 843
Size: 3839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3839
FspI TGC'GCA
Cuts at: 1620 1620
Size: 3839
HaeII r_GCGC'y
Cuts at: 247 388 1273 1521 2543 3522 247
Size: 141 885 248 1022 979 564
Fragments arranged by size:
1022 979 885 564 248 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 436 468 1681 1711 1713 1743 436
Size: 32 1213 30 2 30 2532
Fragments arranged by size:
2532 1213 32 30 30 2
HincII GTy'rAC
Cuts at: 126 878 126
Size: 752 3087
KasI G'GCGC_C
Cuts at: 243 1269 1517 243
Size: 1026 248 2565
Fragments arranged by size:
2565 1026 248
KpnI G_GTAC'C
Cuts at: 859 859
Size: 3839
MluI A'CGCG_T
Cuts at: 3272 3272
Size: 3839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 622 2510 2694 335
Size: 287 1888 184 1480
Fragments arranged by size:
1888 1480 287 184
MscI TGG'CCA
Cuts at: 1600 1600
Size: 3839
MslI CAynn'nnrTG
Cuts at: 1828 1955 1828
Size: 127 3712
MspA1I CmG'CkG
Cuts at: 950 1264 1624 2637 2882 950
Size: 314 360 1013 245 1907
Fragments arranged by size:
1907 1013 360 314 245
NaeI GCC'GGC
Cuts at: 153 2020 153
Size: 1867 1972
NarI GG'CG_CC
Cuts at: 244 1270 1518 244
Size: 1026 248 2565
Fragments arranged by size:
2565 1026 248
NcoI C'CATG_G
Cuts at: 526 526
Size: 3839
NgoMIV G'CCGG_C
Cuts at: 151 2018 151
Size: 1867 1972
NspI r_CATG'y
Cuts at: 892 1392 2299 892
Size: 500 907 2432
Fragments arranged by size:
2432 907 500
PasI CC'CwG_GG
Cuts at: 663 663
Size: 3839
PciI A'CATG_T
Cuts at: 2295 2295
Size: 3839
PflMI CCAn_nnn'nTGG
Cuts at: 219 219
Size: 3839
PmeI GTTT'AAAC
Cuts at: 838 838
Size: 3839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 354 2978 3010 322
Size: 32 2624 32 1151
Fragments arranged by size:
2624 1151 32 32
PspOMI G'GGCC_C
Cuts at: 229 229
Size: 3839
PstI C_TGCA'G
Cuts at: 886 1571 886
Size: 685 3154
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3839
PvuII CAG'CTG
Cuts at: 1264 1624 1264
Size: 360 3479
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 3839
SacI G_AGCT'C
Cuts at: 115 853 115
Size: 738 3101
SalI G'TCGA_C
Cuts at: 876 876
Size: 3839
SapI GCTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
SbfI CC_TGCA'GG
Cuts at: 886 886
Size: 3839
ScaI AGT'ACT
Cuts at: 3356 3356
Size: 3839
SfcI C'TryA_G
Cuts at: 33 882 1567 2560 2751 33
Size: 849 685 993 191 1121
Fragments arranged by size:
1121 993 849 685 191
SfoI GGC'GCC
Cuts at: 245 1271 1519 245
Size: 1026 248 2565
Fragments arranged by size:
2565 1026 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
SmaI CCC'GGG
Cuts at: 861 861
Size: 3839
SmlI C'TyrA_G
Cuts at: 994 1909 2401 2663 994
Size: 915 492 262 2170
Fragments arranged by size:
2170 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3839
SphI G_CATG'C
Cuts at: 892 1392 892
Size: 500 3339
StyI C'CwwG_G
Cuts at: 526 590 973 1327 526
Size: 64 383 354 3038
Fragments arranged by size:
3038 383 354 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 1422 1555 2014 2223 688
Size: 734 133 459 209 2304
Fragments arranged by size:
2304 734 459 209 133
TatI w'GTAC_w
Cuts at: 3354 3354
Size: 3839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 543 575 3356 3388 283
Size: 32 228 32 2781 32 734
Fragments arranged by size:
2781 734 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1636 1636
Size: 3839
XbaI T'CTAG_A
Cuts at: 870 870
Size: 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1334 1334
Size: 3839
XmaI C'CCGG_G
Cuts at: 859 859
Size: 3839
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI