Restriction map for
pF1KB8907
(Circular) (Six-base) MAPSORT of: pF1KB8907.seq Check: 6445 from: 1 to: 3830
pF1KB8907 3830 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 313 1655 2087 2834 3102 200
Size: 113 1342 432 747 268 928
Fragments arranged by size:
1342 928 747 432 268 113
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 345 1135 2702 345
Size: 790 1567 1473
Fragments arranged by size:
1567 1473 790
ApaI G_GGCC'C
Cuts at: 589 589
Size: 3830
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 850 850
Size: 3830
BamHI G'GATC_C
Cuts at: 855 855
Size: 3830
BanI G'GyrC_C
Cuts at: 229 846 1260 1508 1543 229
Size: 617 414 248 35 2516
Fragments arranged by size:
2516 617 414 248 35
BanII G_rGCy'C
Cuts at: 463 589 600 844 1874 463
Size: 126 11 244 1030 2419
Fragments arranged by size:
2419 1030 244 126 11
BbeI G_GCGC'C
Cuts at: 233 1264 1512 233
Size: 1031 248 2551
Fragments arranged by size:
2551 1031 248
BbsI GAAGACnn'nnnn_
Cuts at: 178 178
Size: 3830
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 452 452
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 589 1455 1548 2604 589
Size: 866 93 1056 1815
Fragments arranged by size:
1815 1056 866 93
BmrI ACTGGGnnnn_n'
Cuts at: 627 1042 1123 1458 3602 627
Size: 415 81 335 2144 855
Fragments arranged by size:
2144 855 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 711 1006 1885 2377 2675 456
Size: 255 295 879 492 298 1611
Fragments arranged by size:
1611 879 492 298 295 255
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 133 230 250 1261 1509 2211 3707 133
Size: 97 20 1011 248 702 1496 256
Fragments arranged by size:
1496 1011 702 256 248 97 20
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 406
Size: 3830
BseYI C'CCAG_C
Cuts at: 216 485 565 768 1255 2013 2590 3086 216
Size: 269 80 203 487 758 577 496 960
Fragments arranged by size:
960 758 577 496 487 269 203 80
BsiEI CG_ry'CG
Cuts at: 104 519 1372 1418 2626 104
Size: 415 853 46 1208 1308
Fragments arranged by size:
1308 1208 853 415 46
BsiHKAI G_wGCw'C
Cuts at: 844 1622 1812 2604 844
Size: 778 190 792 2070
Fragments arranged by size:
2070 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 463 589 600 844 1455 1548 1622 1812 1874
Size: 126 11 244 611 93 74 190 62
Cuts at: 1874 2604 463
Size: 730 1689
Fragments arranged by size:
1689 730 611 244 190 126 93 74
62 11
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 320 880 1094 1396 1777 149
Size: 171 560 214 302 381 2202
Fragments arranged by size:
2202 560 381 302 214 171
BsrBI CCG'CTC
Cuts at: 50 396 470 2122 2176 3660 3825 50
Size: 346 74 1652 54 1484 165 55
Fragments arranged by size:
1652 1484 346 165 74 55 54
BsrDI GCAATG_nn'
Cuts at: 414 1742 414
Size: 1328 2502
BsrFI r'CCGG_y
Cuts at: 259 518 1828 2009 2218 259
Size: 259 1310 181 209 1871
Fragments arranged by size:
1871 1310 259 209 181
BssSI C'ACGA_G
Cuts at: 2101 2459 2101
Size: 358 3472
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstEII G'GTnAC_C
Cuts at: 348 348
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 3830
BstYI r'GATC_y
Cuts at: 1 332 855 1322 1345 1680 1926 2938 3024
Size: 331 523 467 23 335 246 1012 86
Cuts at: 3024 3036 3382 1
Size: 12 346 449
Fragments arranged by size:
1012 523 467 449 346 335 331 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 119 192 107
Size: 12 73 3745
Fragments arranged by size:
3745 73 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 380 805 1073 380
Size: 425 268 3137
Fragments arranged by size:
3137 425 268
ClaI AT'CG_AT
Cuts at: 323 323
Size: 3830
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 123 1536 2394 123
Size: 1413 858 1559
Fragments arranged by size:
1559 1413 858
EaeI y'GGCC_r
Cuts at: 261 1415 1589 1980 2007 3352 261
Size: 1154 174 391 27 1345 739
Fragments arranged by size:
1345 1154 739 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 313 1655 2087 2834 3102 200
Size: 113 1342 432 747 268 928
Fragments arranged by size:
1342 928 747 432 268 113
EcoO109I rG'GnC_Cy
Cuts at: 585 969 585
Size: 384 3446
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 789 1849 789
Size: 1060 2770
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 233 292 1264 1512 2534 3513 233
Size: 59 972 248 1022 979 550
Fragments arranged by size:
1022 979 972 550 248 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 1672 1702 1704 1734 517
Size: 32 1123 30 2 30 2613
Fragments arranged by size:
2613 1123 32 30 30 2
HincII GTy'rAC
Cuts at: 407 869 407
Size: 462 3368
KasI G'GCGC_C
Cuts at: 229 1260 1508 229
Size: 1031 248 2551
Fragments arranged by size:
2551 1031 248
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 2501 2685 368
Size: 2133 184 1513
Fragments arranged by size:
2133 1513 184
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 325 1819 1946 325
Size: 1494 127 2209
Fragments arranged by size:
2209 1494 127
MspA1I CmG'CkG
Cuts at: 194 345 485 941 1255 1615 2628 2873 194
Size: 151 140 456 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 456 360 314 245 151 140
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 230 1261 1509 230
Size: 1031 248 2551
Fragments arranged by size:
2551 1031 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PasI CC'CwG_GG
Cuts at: 244 712 244
Size: 468 3362
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PflMI CCAn_nnn'nTGG
Cuts at: 270 270
Size: 3830
PfoI T'CCnGG_A
Cuts at: 548 548
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PspOMI G'GGCC_C
Cuts at: 585 585
Size: 3830
PstI C_TGCA'G
Cuts at: 341 877 1562 341
Size: 536 685 2609
Fragments arranged by size:
2609 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 345 485 1255 1615 345
Size: 140 770 360 2560
Fragments arranged by size:
2560 770 360 140
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SacII CC_GC'GG
Cuts at: 195 195
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 337 873 1558 2551 2742 33
Size: 304 536 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 304 191
SfoI GGC'GCC
Cuts at: 231 1262 1510 231
Size: 1031 248 2551
Fragments arranged by size:
2551 1031 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 471 690 985 1900 2392 2654 471
Size: 219 295 915 492 262 1647
Fragments arranged by size:
1647 915 492 295 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StyI C'CwwG_G
Cuts at: 107 964 1318 107
Size: 857 354 2619
Fragments arranged by size:
2619 857 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 3345 3345
Size: 3830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 529 1627 529
Size: 1098 2732
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 657 1325 519
Size: 138 668 3024
Fragments arranged by size:
3024 668 138
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI