Restriction map for
pF1KB8901
(Circular) (Six-base) MAPSORT of: pF1KB8901.seq Check: 6783 from: 1 to: 3812
pF1KB8901 3812 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 572 850 572
Size: 278 3534
Acc65I G'GTAC_C
Cuts at: 407 828 407
Size: 421 3391
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1637 2069 2816 3084 780
Size: 857 432 747 268 1508
Fragments arranged by size:
1508 857 747 432 268
AfeI AGC'GCT
Cuts at: 374 374
Size: 3812
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 1117 2684 1117
Size: 1567 2245
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 816 3165 3421 816
Size: 2349 256 1207
Fragments arranged by size:
2349 1207 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 338 832 338
Size: 494 3318
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 391 424 665 698 391
Size: 33 241 33 3505
Fragments arranged by size:
3505 241 33 33
BamHI G'GATC_C
Cuts at: 650 837 650
Size: 187 3625
BanI G'GyrC_C
Cuts at: 278 407 828 1242 1490 1525 278
Size: 129 421 414 248 35 2565
Fragments arranged by size:
2565 421 414 248 129 35
BanII G_rGCy'C
Cuts at: 790 826 1856 790
Size: 36 1030 2746
Fragments arranged by size:
2746 1030 36
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BbvCI CC'TCA_GC
Cuts at: 376 376
Size: 3812
BciVI GTATCCnnnnn_n'
Cuts at: 163 666 1710 2471 3812 163
Size: 503 1044 761 1341 163
Fragments arranged by size:
1341 1044 761 503 163
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 722 722
Size: 3812
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 555 1437 1530 2586 555
Size: 882 93 1056 1781
Fragments arranged by size:
1781 1056 882 93
BmrI ACTGGGnnnn_n'
Cuts at: 245 525 1024 1105 1440 3584 245
Size: 280 499 81 335 2144 473
Fragments arranged by size:
2144 499 473 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 198
Size: 3812
Bpu10I CC'TnA_GC
Cuts at: 376 376
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1867 2359 2657 988
Size: 879 492 298 2143
Fragments arranged by size:
2143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 3516 238
Size: 3278 534
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 1243 1491 2193 3689 1243
Size: 248 702 1496 1366
Fragments arranged by size:
1496 1366 702 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 130
Size: 30 3782
BseYI C'CCAG_C
Cuts at: 437 668 783 1237 1995 2572 3068 437
Size: 231 115 454 758 577 496 1181
Fragments arranged by size:
1181 758 577 496 454 231 115
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 826 1604 1794 2586 826
Size: 778 190 792 2052
Fragments arranged by size:
2052 792 778 190
BsmI GAATG_Cn'
Cuts at: 418 418
Size: 3812
Bsp1286I G_dGCh'C
Cuts at: 555 790 826 1437 1530 1604 1794 1856 2586
Size: 235 36 611 93 74 190 62 730
Cuts at: 2586 555
Size: 1781
Fragments arranged by size:
1781 730 611 235 190 93 74 62
36
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 576 862 1076 1378 1759 576
Size: 286 214 302 381 2629
Fragments arranged by size:
2629 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 410 769 1810 1991 2200 410
Size: 359 1041 181 209 2022
Fragments arranged by size:
2022 1041 359 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 650 837 1304 1327 1662 1908 2920 3006
Size: 649 187 467 23 335 246 1012 86
Cuts at: 3006 3018 3364 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 246 187
86 23 12
BtgI C'CryG_G
Cuts at: 296 353 296
Size: 57 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 315 617 767 1397 1571 1962 1989 3334 315
Size: 302 150 630 174 391 27 1345 793
Fragments arranged by size:
1345 793 630 391 302 174 150 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 194 502 799 1835 2045 194
Size: 308 297 1036 210 1961
Fragments arranged by size:
1961 1036 308 297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 780 1637 2069 2816 3084 198
Size: 582 857 432 747 268 926
Fragments arranged by size:
926 857 747 582 432 268
EcoO109I rG'GnC_Cy
Cuts at: 216 951 216
Size: 735 3077
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 376 1246 1494 2516 3495 376
Size: 870 248 1022 979 693
Fragments arranged by size:
1022 979 870 693 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 418 450 482 1654 1684 1686 1716
Size: 32 256 32 32 1172 30 2 30
Cuts at: 1716 130
Size: 2226
Fragments arranged by size:
2226 1172 256 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 138 851 138
Size: 713 3099
HindIII A'AGCT_T
Cuts at: 154 154
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 411 832 411
Size: 421 3391
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 580 781 2483 2667 580
Size: 201 1702 184 1725
Fragments arranged by size:
1725 1702 201 184
MscI TGG'CCA
Cuts at: 317 619 1573 317
Size: 302 954 2556
Fragments arranged by size:
2556 954 302
MslI CAynn'nnrTG
Cuts at: 763 1801 1928 763
Size: 1038 127 2647
Fragments arranged by size:
2647 1038 127
MspA1I CmG'CkG
Cuts at: 176 298 743 923 1237 1597 2610 2855 176
Size: 122 445 180 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 445 360 314 245 180 122
NaeI GCC'GGC
Cuts at: 771 1993 771
Size: 1222 2590
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NcoI C'CATG_G
Cuts at: 353 353
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 769 1991 769
Size: 1222 2590
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PasI CC'CwG_GG
Cuts at: 454 454
Size: 3812
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PfoI T'CCnGG_A
Cuts at: 500 653 500
Size: 153 3659
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 176 1237 1597 176
Size: 1061 360 2391
Fragments arranged by size:
2391 1061 360
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SacII CC_GC'GG
Cuts at: 299 299
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 855 1540 2533 2724 33
Size: 822 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 191
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 340 834 340
Size: 494 3318
SmlI C'TyrA_G
Cuts at: 967 1882 2374 2636 967
Size: 915 492 262 2143
Fragments arranged by size:
2143 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StyI C'CwwG_G
Cuts at: 353 646 946 1300 353
Size: 293 300 354 2865
Fragments arranged by size:
2865 354 300 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 1528 1987 2196 1395
Size: 133 459 209 3011
Fragments arranged by size:
3011 459 209 133
TatI w'GTAC_w
Cuts at: 3327 3327
Size: 3812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 338 832 338
Size: 494 3318
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI