Restriction map for
pF1KB8899
(Circular) (Six-base) MAPSORT of: pF1KB8899.seq Check: 8148 from: 1 to: 3812
pF1KB8899 3812 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2069 2816 3084 1637
Size: 432 747 268 2365
Fragments arranged by size:
2365 747 432 268
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 610 642 610
Size: 32 3780
AlwNI CAG_nnn'CTG
Cuts at: 609 1117 2684 609
Size: 508 1567 1737
Fragments arranged by size:
1737 1567 508
ApaI G_GGCC'C
Cuts at: 403 403
Size: 3812
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 816 3165 3421 816
Size: 2349 256 1207
Fragments arranged by size:
2349 1207 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 139 378 403 427 832 139
Size: 239 25 24 405 3119
Fragments arranged by size:
3119 405 239 25 24
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 367 487 572 653 828 1242 1490 1525 367
Size: 120 85 81 175 414 248 35 2654
Fragments arranged by size:
2654 414 248 175 120 85 81 35
BanII G_rGCy'C
Cuts at: 403 511 826 1856 403
Size: 108 315 1030 2359
Fragments arranged by size:
2359 1030 315 108
BbeI G_GCGC'C
Cuts at: 371 491 576 657 1246 1494 371
Size: 120 85 81 589 248 2689
Fragments arranged by size:
2689 589 248 120 85 81
BbvCI CC'TCA_GC
Cuts at: 211 211
Size: 3812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 651 685 651
Size: 34 3778
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglII A'GATC_T
Cuts at: 707 1327 3364 707
Size: 620 2037 1155
Fragments arranged by size:
2037 1155 620
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 403 1437 1530 2586 403
Size: 1034 93 1056 1629
Fragments arranged by size:
1629 1056 1034 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 637 631
Size: 6 3806
Bpu10I CC'TnA_GC
Cuts at: 211 211
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1867 2359 2657 988
Size: 879 492 298 2143
Fragments arranged by size:
2143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 3812
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 706 1326 3363 6
Size: 700 620 2037 455
Fragments arranged by size:
2037 700 620 455
BsaHI Gr'CG_yC
Cuts at: 238 316 368 488 573 654 1243 1491 2193
Size: 78 52 120 85 81 589 248 702
Cuts at: 2193 3689 238
Size: 1496 361
Fragments arranged by size:
1496 702 589 361 248 120 85 81
78 52
BsaWI w'CCGG_w
Cuts at: 494 1001 1522 2200 2474 2621 3032 494
Size: 507 521 678 274 147 411 1274
Fragments arranged by size:
1274 678 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 3782
BseYI C'CCAG_C
Cuts at: 151 772 1237 1995 2572 3068 151
Size: 621 465 758 577 496 895
Fragments arranged by size:
895 758 621 577 496 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 400
Size: 3812
BsiEI CG_ry'CG
Cuts at: 104 495 1354 1400 2608 104
Size: 391 859 46 1208 1308
Fragments arranged by size:
1308 1208 859 391 46
BsiHKAI G_wGCw'C
Cuts at: 826 1604 1794 2586 826
Size: 778 190 792 2052
Fragments arranged by size:
2052 792 778 190
BsiWI C'GTAC_G
Cuts at: 230 230
Size: 3812
Bsp1286I G_dGCh'C
Cuts at: 403 511 826 1437 1530 1604 1794 1856 2586
Size: 108 315 611 93 74 190 62 730
Cuts at: 2586 403
Size: 1629
Fragments arranged by size:
1629 730 611 315 190 108 93 74
62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 862 1076 1378 1759 422
Size: 440 214 302 381 2475
Fragments arranged by size:
2475 440 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 336 504 2083 2441 336
Size: 168 1579 358 1707
Fragments arranged by size:
1707 1579 358 168
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 707 837 1304 1327 1662 1908 2920 3006
Size: 706 130 467 23 335 246 1012 86
Cuts at: 3006 3018 3364 1
Size: 12 346 449
Fragments arranged by size:
1012 706 467 449 346 335 246 130
86 23 12
BtgI C'CryG_G
Cuts at: 567 567
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 390 1055 390
Size: 665 3147
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 156 1397 1571 1962 1989 3334 156
Size: 1241 174 391 27 1345 634
Fragments arranged by size:
1345 1241 634 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 637 1637 2069 2816 3084 631
Size: 6 1000 432 747 268 1359
Fragments arranged by size:
1359 1000 747 432 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 190 190
Size: 3812
EcoO109I rG'GnC_Cy
Cuts at: 215 332 357 399 400 951 215
Size: 117 25 42 1 551 3076
Fragments arranged by size:
3076 551 117 42 25 1
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 371 491 576 657 699 1246 1494 2516 3495
Size: 120 85 81 42 547 248 1022 979
Cuts at: 3495 371
Size: 688
Fragments arranged by size:
1022 979 688 547 248 120 85 81
42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1654 1684 1686 1716 1654
Size: 30 2 30 3750
Fragments arranged by size:
3750 30 30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
KasI G'GCGC_C
Cuts at: 367 487 572 653 1242 1490 367
Size: 120 85 81 589 248 2689
Fragments arranged by size:
2689 589 248 120 85 81
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 2483 2667 314
Size: 2169 184 1459
Fragments arranged by size:
2169 1459 184
MscI TGG'CCA
Cuts at: 1573 1573
Size: 3812
MslI CAynn'nnrTG
Cuts at: 1801 1928 1801
Size: 127 3685
MspA1I CmG'CkG
Cuts at: 155 180 458 531 776 923 1237 1597 2610
Size: 25 278 73 245 147 314 360 1013
Cuts at: 2610 2855 155
Size: 245 1112
Fragments arranged by size:
1112 1013 360 314 278 245 245 147
73 25
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 368 488 573 654 1243 1491 368
Size: 120 85 81 589 248 2689
Fragments arranged by size:
2689 589 248 120 85 81
NcoI C'CATG_G
Cuts at: 567 567
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 3812
PfoI T'CCnGG_A
Cuts at: 248 248
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 610 642 2951 2983 610
Size: 32 2309 32 1439
Fragments arranged by size:
2309 1439 32 32
PpuMI rG'GwC_Cy
Cuts at: 357 357
Size: 3812
PspOMI G'GGCC_C
Cuts at: 399 399
Size: 3812
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 180 1237 1597 180
Size: 1057 360 2395
Fragments arranged by size:
2395 1057 360
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 855 1540 2533 2724 33
Size: 822 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 191
SfoI GGC'GCC
Cuts at: 369 489 574 655 1244 1492 369
Size: 120 85 81 589 248 2689
Fragments arranged by size:
2689 589 248 120 85 81
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 141 405 834 141
Size: 264 429 3119
Fragments arranged by size:
3119 429 264
SmlI C'TyrA_G
Cuts at: 967 1882 2374 2636 967
Size: 915 492 262 2143
Fragments arranged by size:
2143 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StyI C'CwwG_G
Cuts at: 520 567 946 1300 520
Size: 47 379 354 3032
Fragments arranged by size:
3032 379 354 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 285 1395 1528 1987 2196 285
Size: 1110 133 459 209 1901
Fragments arranged by size:
1901 1110 459 209 133
TatI w'GTAC_w
Cuts at: 437 500 3327 437
Size: 63 2827 922
Fragments arranged by size:
2827 922 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 1307 297
Size: 1010 2802
XmaI C'CCGG_G
Cuts at: 139 403 832 139
Size: 264 429 3119
Fragments arranged by size:
3119 429 264
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 3812
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BseRI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI