Restriction map for
pF1KB8897
(Circular) (Six-base) MAPSORT of: pF1KB8897.seq Check: 8871 from: 1 to: 3785
pF1KB8897 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 488 823 488
Size: 335 3450
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1610 2042 2789 3057 1610
Size: 432 747 268 2338
Fragments arranged by size:
2338 747 432 268
AflIII A'CryG_T
Cuts at: 568 1137 2241 3218 568
Size: 569 1104 977 1135
Fragments arranged by size:
1135 1104 977 569
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 582 1090 2657 582
Size: 508 1567 1710
Fragments arranged by size:
1710 1567 508
ApaI G_GGCC'C
Cuts at: 539 539
Size: 3785
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 510 789 3138 3394 510
Size: 279 2349 256 901
Fragments arranged by size:
2349 901 279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 409 805 409
Size: 396 3389
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 315 336 382 801 1215 1463 1498 315
Size: 21 46 419 414 248 35 2602
Fragments arranged by size:
2602 419 414 248 46 35 21
BanII G_rGCy'C
Cuts at: 539 799 1829 539
Size: 260 1030 2495
Fragments arranged by size:
2495 1030 260
BbeI G_GCGC'C
Cuts at: 319 340 386 1219 1467 319
Size: 21 46 833 248 2637
Fragments arranged by size:
2637 833 248 46 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 624 658 624
Size: 34 3751
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglI GCCn_nnn'nGGC
Cuts at: 237 551 237
Size: 314 3471
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 730 897 730
Size: 167 3618
Bme1580I G_kGCm'C
Cuts at: 539 1410 1503 2559 539
Size: 871 93 1056 1765
Fragments arranged by size:
1765 1056 871 93
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 610 604
Size: 6 3779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 316 337 383 1216 1464 2166 3662 316
Size: 21 46 833 248 702 1496 439
Fragments arranged by size:
1496 833 702 439 248 46 21
BsaWI w'CCGG_w
Cuts at: 483 974 1495 2173 2447 2594 3005 483
Size: 491 521 678 274 147 411 1263
Fragments arranged by size:
1263 678 521 491 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 583 613 511
Size: 30 42 30 3683
Fragments arranged by size:
3683 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 772 769
Size: 3 3782
BseYI C'CCAG_C
Cuts at: 538 1210 1968 2545 3041 538
Size: 672 758 577 496 1282
Fragments arranged by size:
1282 758 672 577 496
BsiEI CG_ry'CG
Cuts at: 104 309 546 1327 1373 2581 104
Size: 205 237 781 46 1208 1308
Fragments arranged by size:
1308 1208 781 237 205 46
BsiHKAI G_wGCw'C
Cuts at: 478 799 1577 1767 2559 478
Size: 321 778 190 792 1704
Fragments arranged by size:
1704 792 778 321 190
BsmBI CGTCTCn'nnnn_
Cuts at: 743 743
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 478 539 799 1410 1503 1577 1767 1829 2559
Size: 61 260 611 93 74 190 62 730
Cuts at: 2559 478
Size: 1704
Fragments arranged by size:
1704 730 611 260 190 93 74 62
61
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 313 2077 2131 3615 3780 50
Size: 136 127 1764 54 1484 165 55
Fragments arranged by size:
1764 1484 165 136 127 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 288 333 339 545 1783 1964 2173 288
Size: 45 6 206 1238 181 209 1900
Fragments arranged by size:
1900 1238 209 206 181 45 6
BssHII G'CGCG_C
Cuts at: 431 431
Size: 3785
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstYI r'GATC_y
Cuts at: 1 810 1277 1300 1635 1881 2893 2979 2991
Size: 809 467 23 335 246 1012 86 12
Cuts at: 2991 3337 1
Size: 346 449
Fragments arranged by size:
1012 809 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 415 493 415
Size: 78 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 149 306 543 1370 1544 1935 1962 3307 149
Size: 157 237 827 174 391 27 1345 627
Fragments arranged by size:
1345 827 627 391 237 174 157 27
EagI C'GGCC_G
Cuts at: 306 543 1370 306
Size: 237 827 2721
Fragments arranged by size:
2721 827 237
EarI CTCTTCn'nnn_
Cuts at: 458 1808 2018 458
Size: 1350 210 2225
Fragments arranged by size:
2225 1350 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 610 1610 2042 2789 3057 604
Size: 6 1000 432 747 268 1332
Fragments arranged by size:
1332 1000 747 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 924 924
Size: 3785
EcoRI G'AATT_C
Cuts at: 510 789 510
Size: 279 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 372 1566 372
Size: 1194 2591
HaeII r_GCGC'y
Cuts at: 319 340 386 1219 1467 2489 3468 319
Size: 21 46 833 248 1022 979 636
Fragments arranged by size:
1022 979 833 636 248 46 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 1627 1657 1659 1689 346
Size: 32 1249 30 2 30 2442
Fragments arranged by size:
2442 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 315 336 382 1215 1463 315
Size: 21 46 833 248 2637
Fragments arranged by size:
2637 833 248 46 21
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 2456 2640 404
Size: 2052 184 1549
Fragments arranged by size:
2052 1549 184
MscI TGG'CCA
Cuts at: 151 1546 151
Size: 1395 2390
MslI CAynn'nnrTG
Cuts at: 1774 1901 1774
Size: 127 3658
MspA1I CmG'CkG
Cuts at: 155 213 417 504 542 896 1210 1570 2583
Size: 58 204 87 38 354 314 360 1013
Cuts at: 2583 2828 155
Size: 245 1112
Fragments arranged by size:
1112 1013 360 354 314 245 204 87
58 38
NaeI GCC'GGC
Cuts at: 290 335 341 547 1966 290
Size: 45 6 206 1419 2109
Fragments arranged by size:
2109 1419 206 45 6
NarI GG'CG_CC
Cuts at: 316 337 383 1216 1464 316
Size: 21 46 833 248 2637
Fragments arranged by size:
2637 833 248 46 21
NgoMIV G'CCGG_C
Cuts at: 288 333 339 545 1964 288
Size: 45 6 206 1419 2109
Fragments arranged by size:
2109 1419 206 45 6
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PflMI CCAn_nnn'nTGG
Cuts at: 582 582
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PspOMI G'GGCC_C
Cuts at: 535 535
Size: 3785
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SacII CC_GC'GG
Cuts at: 418 418
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 458 1808 2018 458
Size: 1350 210 2225
Fragments arranged by size:
2225 1350 210
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 551 551
Size: 3785
SfoI GGC'GCC
Cuts at: 317 338 384 1217 1465 317
Size: 21 46 833 248 2637
Fragments arranged by size:
2637 833 248 46 21
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SgrAI Cr'CCGG_yG
Cuts at: 288 288
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 940 1855 2347 2609 940
Size: 915 492 262 2116
Fragments arranged by size:
2116 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 919 1273 919
Size: 354 3431
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI