Restriction map for
pF1KB8895
(Circular) (Six-base) MAPSORT of: pF1KB8895.seq Check: 6714 from: 1 to: 3782
pF1KB8895 3782 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 558 820 558
Size: 262 3520
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1607 2039 2786 3054 317
Size: 1290 432 747 268 1045
Fragments arranged by size:
1290 1045 747 432 268
AflIII A'CryG_T
Cuts at: 611 1134 2238 3215 611
Size: 523 1104 977 1178
Fragments arranged by size:
1178 1104 977 523
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 1087 2654 1087
Size: 1567 2215
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 304 580 786 3135 3391 304
Size: 276 206 2349 256 695
Fragments arranged by size:
2349 695 276 256 206
AseI AT'TA_AT
Cuts at: 20 166 20
Size: 146 3636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 802 802
Size: 3782
BamHI G'GATC_C
Cuts at: 502 807 502
Size: 305 3477
BanI G'GyrC_C
Cuts at: 798 1212 1460 1495 798
Size: 414 248 35 3085
Fragments arranged by size:
3085 414 248 35
BanII G_rGCy'C
Cuts at: 289 796 1826 289
Size: 507 1030 2245
Fragments arranged by size:
2245 1030 507
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 1407 1500 2556 1407
Size: 93 1056 2633
Fragments arranged by size:
2633 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 114 994 1075 1410 3554 114
Size: 880 81 335 2144 342
Fragments arranged by size:
2144 880 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 619
Size: 30 3752
BseYI C'CCAG_C
Cuts at: 1207 1965 2542 3038 1207
Size: 758 577 496 1951
Fragments arranged by size:
1951 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1574 1764 2556 796
Size: 778 190 792 2022
Fragments arranged by size:
2022 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 289 796 1407 1500 1574 1764 1826 2556 289
Size: 507 611 93 74 190 62 730 1515
Fragments arranged by size:
1515 730 611 507 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 713 1159 713
Size: 446 3336
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstXI CCAn_nnnn'nTGG
Cuts at: 499 547 499
Size: 48 3734
BstYI r'GATC_y
Cuts at: 1 502 700 807 1274 1297 1632 1878 2890
Size: 501 198 107 467 23 335 246 1012
Cuts at: 2890 2976 2988 3334 1
Size: 86 12 346 449
Fragments arranged by size:
1012 501 467 449 346 335 246 198
107 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 711 1025 711
Size: 314 3468
DraI TTT'AAA
Cuts at: 465 781 465
Size: 316 3466
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 254 1367 1541 1932 1959 3304 254
Size: 1113 174 391 27 1345 732
Fragments arranged by size:
1345 1113 732 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 729 1805 2015 729
Size: 1076 210 2496
Fragments arranged by size:
2496 1076 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1607 2039 2786 3054 317
Size: 1290 432 747 268 1045
Fragments arranged by size:
1290 1045 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 921 921
Size: 3782
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
EcoRV GAT'ATC
Cuts at: 195 195
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 1801 258
Size: 1543 2239
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 228 1216 1464 2486 3465 228
Size: 988 248 1022 979 545
Fragments arranged by size:
1022 988 979 545 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 711 743 1624 1654 1656 1686 711
Size: 32 881 30 2 30 2807
Fragments arranged by size:
2807 881 32 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2637 2453
Size: 184 3598
MscI TGG'CCA
Cuts at: 256 1543 256
Size: 1287 2495
MslI CAynn'nnrTG
Cuts at: 151 1771 1898 151
Size: 1620 127 2035
Fragments arranged by size:
2035 1620 127
MspA1I CmG'CkG
Cuts at: 893 1207 1567 2580 2825 893
Size: 314 360 1013 245 1850
Fragments arranged by size:
1850 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NcoI C'CATG_G
Cuts at: 107 107
Size: 3782
NdeI CA'TA_TG
Cuts at: 327 327
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 615 835 1335 2242 615
Size: 220 500 907 2155
Fragments arranged by size:
2155 907 500 220
PciI A'CATG_T
Cuts at: 611 2238 611
Size: 1627 2155
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 405 437 405
Size: 32 3750
PstI C_TGCA'G
Cuts at: 718 829 1514 718
Size: 111 685 2986
Fragments arranged by size:
2986 685 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 1207 1567 1207
Size: 360 3422
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 714 825 1510 2503 2694 33
Size: 681 111 685 993 191 1121
Fragments arranged by size:
1121 993 685 681 191 111
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 391 67
Size: 324 3458
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StuI AGG'CCT
Cuts at: 241 241
Size: 3782
StyI C'CwwG_G
Cuts at: 107 916 1270 107
Size: 809 354 2619
Fragments arranged by size:
2619 809 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 675 3297 675
Size: 2622 1160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 1277
Size: 3782
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
XmnI GAAnn'nnTTC
Cuts at: 167 167
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI