Restriction map for
pF1KB8892
(Circular) (Six-base) MAPSORT of: pF1KB8892.seq Check: 8502 from: 1 to: 3758
pF1KB8892 3758 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2015 2762 3030 1583
Size: 432 747 268 2311
Fragments arranged by size:
2311 747 432 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaI G_GGCC'C
Cuts at: 685 685
Size: 3758
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 762 3111 3367 762
Size: 2349 256 1153
Fragments arranged by size:
2349 1153 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 352 583 778 352
Size: 231 195 3332
Fragments arranged by size:
3332 231 195
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 253 352 685 772 1802 253
Size: 99 333 87 1030 2209
Fragments arranged by size:
2209 1030 333 99 87
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglI GCCn_nnn'nGGC
Cuts at: 383 595 383
Size: 212 3546
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 247 870 247
Size: 623 3135
Bme1580I G_kGCm'C
Cuts at: 685 1383 1476 2532 685
Size: 698 93 1056 1911
Fragments arranged by size:
1911 1056 698 93
BmgBI CAC'GTC
Cuts at: 552 552
Size: 3758
BmrI ACTGGGnnnn_n'
Cuts at: 679 970 1051 1386 3530 679
Size: 291 81 335 2144 907
Fragments arranged by size:
2144 907 335 291 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 760 382
Size: 378 3380
Bpu10I CC'TnA_GC
Cuts at: 399 399
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 934 1813 2305 2603 589
Size: 345 879 492 298 1744
Fragments arranged by size:
1744 879 492 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 332 1189 1437 2139 3635 332
Size: 857 248 702 1496 455
Fragments arranged by size:
1496 857 702 455 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 302 378 485 624 1300 1346 2554 104
Size: 198 76 107 139 676 46 1208 1308
Fragments arranged by size:
1308 1208 676 198 139 107 76 46
BsiHKAI G_wGCw'C
Cuts at: 772 1550 1740 2532 772
Size: 778 190 792 1998
Fragments arranged by size:
1998 792 778 190
BsmI GAATG_Cn'
Cuts at: 445 445
Size: 3758
BsmBI CGTCTCn'nnnn_
Cuts at: 414 414
Size: 3758
Bsp1286I G_dGCh'C
Cuts at: 253 352 685 772 1383 1476 1550 1740 1802
Size: 99 333 87 611 93 74 190 62
Cuts at: 1802 2532 253
Size: 730 1479
Fragments arranged by size:
1479 730 611 333 190 99 93 87
74 62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 417 2050 2104 3588 3753 50
Size: 367 1633 54 1484 165 55
Fragments arranged by size:
1633 1484 367 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 386 480 712 1756 1937 2146 386
Size: 94 232 1044 181 209 1998
Fragments arranged by size:
1998 1044 232 209 181 94
BssSI C'ACGA_G
Cuts at: 115 2029 2387 115
Size: 1914 358 1486
Fragments arranged by size:
1914 1486 358
BstAPI GCAn_nnn'nTGC
Cuts at: 171 1135 171
Size: 964 2794
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 783 1250 1273 1608 1854 2866 2952 2964
Size: 782 467 23 335 246 1012 86 12
Cuts at: 2964 3310 1
Size: 346 449
Fragments arranged by size:
1012 782 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 296 296
Size: 3758
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 519 1708 1889 2052 519
Size: 1189 181 163 2225
Fragments arranged by size:
2225 1189 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1001
Size: 3758
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 299 375 384 482 557 621 718 1343 1517
Size: 76 9 98 75 64 97 625 174
Cuts at: 1517 1908 1935 3280 299
Size: 391 27 1345 777
Fragments arranged by size:
1345 777 625 391 174 98 97 76
75 64 27 9
EagI C'GGCC_G
Cuts at: 299 375 482 621 1343 299
Size: 76 107 139 722 2714
Fragments arranged by size:
2714 722 139 107 76
EarI CTCTTCn'nnn_
Cuts at: 196 688 1781 1991 196
Size: 492 1093 210 1963
Fragments arranged by size:
1963 1093 492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 405 628 1966 2276 2422 3568 405
Size: 223 1338 310 146 1146 595
Fragments arranged by size:
1338 1146 595 310 223 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 760 1583 2015 2762 3030 382
Size: 378 823 432 747 268 1110
Fragments arranged by size:
1110 823 747 432 378 268
EcoO109I rG'GnC_Cy
Cuts at: 224 403 897 224
Size: 179 494 3085
Fragments arranged by size:
3085 494 179
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 164 1539 164
Size: 1375 2383
HaeII r_GCGC'y
Cuts at: 370 739 1192 1440 2462 3441 370
Size: 369 453 248 1022 979 687
Fragments arranged by size:
1022 979 687 453 369 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 495 527 556 588 1600 1630 1632 1662 495
Size: 32 29 32 1012 30 2 30 2591
Fragments arranged by size:
2591 1012 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 559 1519 559
Size: 960 2798
MslI CAynn'nnrTG
Cuts at: 1747 1874 1747
Size: 127 3631
MspA1I CmG'CkG
Cuts at: 298 304 869 1183 1543 2556 2801 298
Size: 6 565 314 360 1013 245 1255
Fragments arranged by size:
1255 1013 565 360 314 245 6
NaeI GCC'GGC
Cuts at: 388 482 1939 388
Size: 94 1457 2207
Fragments arranged by size:
2207 1457 94
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NgoMIV G'CCGG_C
Cuts at: 386 480 1937 386
Size: 94 1457 2207
Fragments arranged by size:
2207 1457 94
NotI GC'GGCC_GC
Cuts at: 299 299
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PasI CC'CwG_GG
Cuts at: 678 678
Size: 3758
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 173 205 2897 2929 173
Size: 32 2692 32 1002
Fragments arranged by size:
2692 1002 32 32
PpuMI rG'GwC_Cy
Cuts at: 403 403
Size: 3758
PspOMI G'GGCC_C
Cuts at: 681 681
Size: 3758
PstI C_TGCA'G
Cuts at: 309 653 805 1490 309
Size: 344 152 685 2577
Fragments arranged by size:
2577 685 344 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 1183 1543 1183
Size: 360 3398
RsrII CG'GwC_CG
Cuts at: 196 1953 196
Size: 1757 2001
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SacII CC_GC'GG
Cuts at: 299 299
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SexAI A'CCwGG_T
Cuts at: 749 749
Size: 3758
SfcI C'TryA_G
Cuts at: 33 305 649 801 1486 2479 2670 33
Size: 272 344 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 344 272 191 152
SfiI GGCCn_nnn'nGGCC
Cuts at: 383 383
Size: 3758
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 354 780 354
Size: 426 3332
SmlI C'TyrA_G
Cuts at: 604 913 1828 2320 2582 604
Size: 309 915 492 262 1780
Fragments arranged by size:
1780 915 492 309 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StuI AGG'CCT
Cuts at: 339 589 339
Size: 250 3508
StyI C'CwwG_G
Cuts at: 560 892 1246 560
Size: 332 354 3072
Fragments arranged by size:
3072 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 184 1341 1474 1933 2142 184
Size: 1157 133 459 209 1800
Fragments arranged by size:
1800 1157 459 209 133
TatI w'GTAC_w
Cuts at: 3273 3273
Size: 3758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1253 114
Size: 1139 2619
XmaI C'CCGG_G
Cuts at: 352 778 352
Size: 426 3332
XmnI GAAnn'nnTTC
Cuts at: 665 665
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BsaXI BseRI BsgI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI