Restriction map for
pF1KB8891
(Circular) (Six-base) MAPSORT of: pF1KB8891.seq Check: 9095 from: 1 to: 3746
pF1KB8891 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 411 750 3099 3355 411
Size: 339 2349 256 802
Fragments arranged by size:
2349 802 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 193 766 193
Size: 573 3173
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 198 762 1176 1424 1459 198
Size: 564 414 248 35 2485
Fragments arranged by size:
2485 564 414 248 35
BanII G_rGCy'C
Cuts at: 760 1790 760
Size: 1030 2716
BbeI G_GCGC'C
Cuts at: 202 1180 1428 202
Size: 978 248 2520
Fragments arranged by size:
2520 978 248
BbsI GAAGACnn'nnnn_
Cuts at: 215 215
Size: 3746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 667 633
Size: 34 3712
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 282 1266 282
Size: 984 2762
BglI GCCn_nnn'nGGC
Cuts at: 443 443
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 247 858 247
Size: 611 3135
Bme1580I G_kGCm'C
Cuts at: 1371 1464 2520 1371
Size: 93 1056 2597
Fragments arranged by size:
2597 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 199 1177 1425 2127 3623 199
Size: 978 248 702 1496 322
Fragments arranged by size:
1496 978 702 322 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 225 1288 1334 2542 104
Size: 121 1063 46 1208 1308
Fragments arranged by size:
1308 1208 1063 121 46
BsiHKAI G_wGCw'C
Cuts at: 760 1538 1728 2520 760
Size: 778 190 792 1986
Fragments arranged by size:
1986 792 778 190
BsiWI C'GTAC_G
Cuts at: 572 572
Size: 3746
BsmI GAATG_Cn'
Cuts at: 714 714
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 760 1371 1464 1538 1728 1790 2520 760
Size: 611 93 74 190 62 730 1986
Fragments arranged by size:
1986 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 215 378 2038 2092 3576 3741 50
Size: 165 163 1660 54 1484 165 55
Fragments arranged by size:
1660 1484 165 165 163 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 178 1744 1925 2134 178
Size: 1566 181 209 1790
Fragments arranged by size:
1790 1566 209 181
BssHII G'CGCG_C
Cuts at: 233 233
Size: 3746
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstEII G'GTnAC_C
Cuts at: 338 338
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 569 636 1696 1877 2040 569
Size: 67 1060 181 163 2275
Fragments arranged by size:
2275 1060 181 163 67
BtsI GCAGTG_nn'
Cuts at: 258 365 989 258
Size: 107 624 3015
Fragments arranged by size:
3015 624 107
DraI TTT'AAA
Cuts at: 482 745 482
Size: 263 3483
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 222 1331 1505 1896 1923 3268 222
Size: 1109 174 391 27 1345 700
Fragments arranged by size:
1345 1109 700 391 174 27
EagI C'GGCC_G
Cuts at: 222 1331 222
Size: 1109 2637
EarI CTCTTCn'nnn_
Cuts at: 366 1769 1979 366
Size: 1403 210 2133
Fragments arranged by size:
2133 1403 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 1954 2264 2410 3556 150
Size: 1804 310 146 1146 340
Fragments arranged by size:
1804 1146 340 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 885 885
Size: 3746
EcoRI G'AATT_C
Cuts at: 411 750 411
Size: 339 3407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 202 1180 1428 2450 3429 202
Size: 978 248 1022 979 519
Fragments arranged by size:
1022 979 978 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 1588 1618 1620 1650 655
Size: 32 901 30 2 30 2751
Fragments arranged by size:
2751 901 32 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 198 1176 1424 198
Size: 978 248 2520
Fragments arranged by size:
2520 978 248
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 409 2417 2601 409
Size: 2008 184 1554
Fragments arranged by size:
2008 1554 184
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 482 1735 1862 482
Size: 1253 127 2366
Fragments arranged by size:
2366 1253 127
MspA1I CmG'CkG
Cuts at: 171 397 545 562 857 1171 1531 2544 2789
Size: 226 148 17 295 314 360 1013 245
Cuts at: 2789 171
Size: 1128
Fragments arranged by size:
1128 1013 360 314 295 245 226 148
17
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 199 1177 1425 199
Size: 978 248 2520
Fragments arranged by size:
2520 978 248
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 641 799 1299 2206 641
Size: 158 500 907 2181
Fragments arranged by size:
2181 907 500 158
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PshAI GACnn'nnGTC
Cuts at: 497 497
Size: 3746
PstI C_TGCA'G
Cuts at: 396 544 793 1478 396
Size: 148 249 685 2664
Fragments arranged by size:
2664 685 249 148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 397 562 1171 1531 397
Size: 165 609 360 2612
Fragments arranged by size:
2612 609 360 165
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SexAI A'CCwGG_T
Cuts at: 689 689
Size: 3746
SfcI C'TryA_G
Cuts at: 33 392 540 789 1474 2467 2658 33
Size: 359 148 249 685 993 191 1121
Fragments arranged by size:
1121 993 685 359 249 191 148
SfoI GGC'GCC
Cuts at: 200 1178 1426 200
Size: 978 248 2520
Fragments arranged by size:
2520 978 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
SspI AAT'ATT
Cuts at: 584 584
Size: 3746
StuI AGG'CCT
Cuts at: 189 189
Size: 3746
StyI C'CwwG_G
Cuts at: 515 880 1234 515
Size: 365 354 3027
Fragments arranged by size:
3027 365 354
SwaI ATTT'AAAT
Cuts at: 482 482
Size: 3746
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 3263 3295 142
Size: 32 3089 32 593
Fragments arranged by size:
3089 593 32 32
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 714 714
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsmBI BsrGI BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI XhoI ZraI