Restriction map for
pF1KB8890
(Circular) (Six-base) MAPSORT of: pF1KB8890.seq Check: 9642 from: 1 to: 3737
pF1KB8890 3737 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 775 775
Size: 3737
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AflII C'TTAA_G
Cuts at: 286 286
Size: 3737
AflIII A'CryG_T
Cuts at: 1089 2193 3170 1089
Size: 1104 977 1656
Fragments arranged by size:
1656 1104 977
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 1042 2609 1042
Size: 1567 2170
ApaI G_GGCC'C
Cuts at: 324 324
Size: 3737
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 741 3090 3346 741
Size: 2349 256 1132
Fragments arranged by size:
2349 1132 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 757 757
Size: 3737
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 114 148 348 564 753 1167 1415 1450 114
Size: 34 200 216 189 414 248 35 2401
Fragments arranged by size:
2401 414 248 216 200 189 35 34
BanII G_rGCy'C
Cuts at: 324 751 1781 324
Size: 427 1030 2280
Fragments arranged by size:
2280 1030 427
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3737
BglII A'GATC_T
Cuts at: 1252 3289 1252
Size: 2037 1700
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 151 324 351 1362 1455 2511 151
Size: 173 27 1011 93 1056 1377
Fragments arranged by size:
1377 1056 1011 173 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 152 949 1030 1365 3509 152
Size: 797 81 335 2144 380
Fragments arranged by size:
2144 797 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 104
Size: 3737
Bpu10I CC'TnA_GC
Cuts at: 500 500
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1792 2284 2582 913
Size: 879 492 298 2068
Fragments arranged by size:
2068 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 657 3441 657
Size: 2784 953
BsaAI yAC'GTr
Cuts at: 1090 1090
Size: 3737
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 1168 1416 2118 3614 1168
Size: 248 702 1496 1291
Fragments arranged by size:
1496 1291 702 248
BsaWI w'CCGG_w
Cuts at: 926 1447 2125 2399 2546 2957 926
Size: 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 3707
BseYI C'CCAG_C
Cuts at: 637 1162 1920 2497 2993 637
Size: 525 758 577 496 1381
Fragments arranged by size:
1381 758 577 525 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 751 1529 1719 2511 751
Size: 778 190 792 1977
Fragments arranged by size:
1977 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 324 351 751 1362 1455 1529 1719 1781
Size: 173 27 400 611 93 74 190 62
Cuts at: 1781 2511 151
Size: 730 1377
Fragments arranged by size:
1377 730 611 400 190 173 93 74
62 27
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 527 2913 527
Size: 2386 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 787 1001 1303 1684 787
Size: 214 302 381 2840
Fragments arranged by size:
2840 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2029 2083 3567 3732 50
Size: 1979 54 1484 165 55
Fragments arranged by size:
1979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 428 1649 428
Size: 1221 2516
BsrFI r'CCGG_y
Cuts at: 1735 1916 2125 1735
Size: 181 209 3347
Fragments arranged by size:
3347 209 181
BsrGI T'GTAC_A
Cuts at: 542 542
Size: 3737
BssSI C'ACGA_G
Cuts at: 610 2008 2366 610
Size: 1398 358 1981
Fragments arranged by size:
1981 1398 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstYI r'GATC_y
Cuts at: 1 762 1229 1252 1587 1833 2845 2931 2943
Size: 761 467 23 335 246 1012 86 12
Cuts at: 2943 3289 1
Size: 346 449
Fragments arranged by size:
1012 761 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 1322 1496 1887 1914 3259 1322
Size: 174 391 27 1345 1800
Fragments arranged by size:
1800 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 2255 2401 3547 1945
Size: 310 146 1146 2135
Fragments arranged by size:
2135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 749 749
Size: 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1562 1994 2741 3009 104
Size: 1458 432 747 268 832
Fragments arranged by size:
1458 832 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 175 286 175
Size: 111 3626
EcoO109I rG'GnC_Cy
Cuts at: 320 876 320
Size: 556 3181
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1756 1756
Size: 3737
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 1171 1419 2441 3420 1171
Size: 248 1022 979 1488
Fragments arranged by size:
1488 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 637 669 1579 1609 1611 1641 580
Size: 32 25 32 910 30 2 30 2676
Fragments arranged by size:
2676 910 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 776 776
Size: 3737
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2408 2592 2408
Size: 184 3553
MscI TGG'CCA
Cuts at: 1498 1498
Size: 3737
MslI CAynn'nnrTG
Cuts at: 130 157 562 1726 1853 130
Size: 27 405 1164 127 2014
Fragments arranged by size:
2014 1164 405 127 27
MspA1I CmG'CkG
Cuts at: 637 848 1162 1522 2535 2780 637
Size: 211 314 360 1013 245 1594
Fragments arranged by size:
1594 1013 360 314 245 211
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NcoI C'CATG_G
Cuts at: 107 107
Size: 3737
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PflMI CCAn_nnn'nTGG
Cuts at: 273 408 273
Size: 135 3602
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PspOMI G'GGCC_C
Cuts at: 320 320
Size: 3737
PstI C_TGCA'G
Cuts at: 784 1469 784
Size: 685 3052
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 1162 1522 1162
Size: 360 3377
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 751 751
Size: 3737
SalI G'TCGA_C
Cuts at: 774 774
Size: 3737
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 780 1465 2458 2649 33
Size: 747 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 191
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 759 759
Size: 3737
SmlI C'TyrA_G
Cuts at: 286 892 1807 2299 2561 286
Size: 606 915 492 262 1462
Fragments arranged by size:
1462 915 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
StuI AGG'CCT
Cuts at: 592 592
Size: 3737
StyI C'CwwG_G
Cuts at: 107 871 1225 107
Size: 764 354 2619
Fragments arranged by size:
2619 764 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1453 1912 2121 1320
Size: 133 459 209 2936
Fragments arranged by size:
2936 459 209 133
TatI w'GTAC_w
Cuts at: 542 3252 542
Size: 2710 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 649 1534 649
Size: 885 2852
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 223 726 1232 159
Size: 64 503 506 2664
Fragments arranged by size:
2664 506 503 64
XmaI C'CCGG_G
Cuts at: 757 757
Size: 3737
XmnI GAAnn'nnTTC
Cuts at: 670 670
Size: 3737
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI