Restriction map for
pF1KB8885
(Circular) (Six-base) MAPSORT of: pF1KB8885.seq Check: 7906 from: 1 to: 3692
pF1KB8885 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 165 165
Size: 3692
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1949 2696 2964 1517
Size: 432 747 268 2245
Fragments arranged by size:
2245 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaI G_GGCC'C
Cuts at: 492 492
Size: 3692
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 300 451 712 300
Size: 151 261 3280
Fragments arranged by size:
3280 261 151
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 148 529 708 1122 1370 1405 148
Size: 381 179 414 248 35 2435
Fragments arranged by size:
2435 414 381 248 179 35
BanII G_rGCy'C
Cuts at: 190 288 492 541 706 1736 190
Size: 98 204 49 165 1030 2146
Fragments arranged by size:
2146 1030 204 165 98 49
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3658
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglI GCCn_nnn'nGGC
Cuts at: 283 283
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 492 534 1317 1410 2466 492
Size: 42 783 93 1056 1718
Fragments arranged by size:
1718 1056 783 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 486 556 610 904 985 1320 3464 486
Size: 70 54 294 81 335 2144 714
Fragments arranged by size:
2144 714 335 294 81 70 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2059 204
Size: 1855 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1747 2239 2537 868
Size: 879 492 298 2023
Fragments arranged by size:
2023 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 627 881 1402 2080 2354 2501 2912 627
Size: 254 521 678 274 147 411 1407
Fragments arranged by size:
1407 678 521 411 274 254 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 318 348 318
Size: 30 3662
BseYI C'CCAG_C
Cuts at: 639 1117 1875 2452 2948 639
Size: 478 758 577 496 1383
Fragments arranged by size:
1383 758 577 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 226 346 113
Size: 113 120 3459
Fragments arranged by size:
3459 120 113
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 190 706 1484 1674 2466 190
Size: 516 778 190 792 1416
Fragments arranged by size:
1416 792 778 516 190
Bsp1286I G_dGCh'C
Cuts at: 190 288 492 534 541 706 1317 1410 1484
Size: 98 204 42 7 165 611 93 74
Cuts at: 1484 1674 1736 2466 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 204 190 165 98 93
74 62 42 7
BspEI T'CCGG_A
Cuts at: 627 2912 627
Size: 2285 1407
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 165 368 742 956 1258 1639 165
Size: 203 374 214 302 381 2218
Fragments arranged by size:
2218 381 374 302 214 203
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 304 717 1184 1207 1542 1788 2800 2886
Size: 303 413 467 23 335 246 1012 86
Cuts at: 2886 2898 3244 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 413 346 335 303 246
86 23 12
BtgI C'CryG_G
Cuts at: 191 191
Size: 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 236 662 935 236
Size: 426 273 2993
Fragments arranged by size:
2993 426 273
ClaI AT'CG_AT
Cuts at: 551 551
Size: 3692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 261 296 261
Size: 35 3657
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 233 1277 1451 1842 1869 3214 233
Size: 1044 174 391 27 1345 711
Fragments arranged by size:
1345 1044 711 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 188 704 188
Size: 516 3176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1517 1949 2696 2964 425
Size: 1092 432 747 268 1153
Fragments arranged by size:
1153 1092 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 463 488 831 463
Size: 25 343 3324
Fragments arranged by size:
3324 343 25
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 661 693 1534 1564 1566 1596 661
Size: 32 841 30 2 30 2757
Fragments arranged by size:
2757 841 32 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 587 2363 2547 227
Size: 360 1776 184 1372
Fragments arranged by size:
1776 1372 360 184
MscI TGG'CCA
Cuts at: 235 1453 235
Size: 1218 2474
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 396 803 1117 1477 2490 2735 396
Size: 407 314 360 1013 245 1353
Fragments arranged by size:
1353 1013 407 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PasI CC'CwG_GG
Cuts at: 250 250
Size: 3692
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PpuMI rG'GwC_Cy
Cuts at: 463 463
Size: 3692
PspOMI G'GGCC_C
Cuts at: 488 488
Size: 3692
PstI C_TGCA'G
Cuts at: 401 739 1424 401
Size: 338 685 2669
Fragments arranged by size:
2669 685 338
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 396 1117 1477 396
Size: 721 360 2611
Fragments arranged by size:
2611 721 360
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 190 706 190
Size: 516 3176
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 397 735 1420 2413 2604 33
Size: 364 338 685 993 191 1121
Fragments arranged by size:
1121 993 685 364 338 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 453 714 453
Size: 261 3431
SmlI C'TyrA_G
Cuts at: 847 1762 2254 2516 847
Size: 915 492 262 2023
Fragments arranged by size:
2023 915 492 262
SpeI A'CTAG_T
Cuts at: 67 292 67
Size: 225 3467
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StuI AGG'CCT
Cuts at: 126 126
Size: 3692
StyI C'CwwG_G
Cuts at: 272 826 1180 272
Size: 554 354 2784
Fragments arranged by size:
2784 554 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 626 1275 1408 1867 2076 626
Size: 649 133 459 209 2242
Fragments arranged by size:
2242 649 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 451 712 451
Size: 261 3431
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI