Restriction map for
pF1KB8881
(Circular) (Six-base) MAPSORT of: pF1KB8881.seq Check: 7855 from: 1 to: 3650
pF1KB8881 3650 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1907 2654 2922 1475
Size: 432 747 268 2203
Fragments arranged by size:
2203 747 432 268
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 510 955 2522 510
Size: 445 1567 1638
Fragments arranged by size:
1638 1567 445
ApaI G_GGCC'C
Cuts at: 378 378
Size: 3650
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 196 654 3003 3259 196
Size: 458 2349 256 587
Fragments arranged by size:
2349 587 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 586 670 586
Size: 84 3566
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 291 432 666 1080 1328 1363 291
Size: 141 234 414 248 35 2578
Fragments arranged by size:
2578 414 248 234 141 35
BanII G_rGCy'C
Cuts at: 232 310 378 664 1694 232
Size: 78 68 286 1030 2188
Fragments arranged by size:
2188 1030 286 78 68
BbeI G_GCGC'C
Cuts at: 295 1084 1332 295
Size: 789 248 2613
Fragments arranged by size:
2613 789 248
BbvCI CC'TCA_GC
Cuts at: 124 124
Size: 3650
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglI GCCn_nnn'nGGC
Cuts at: 269 269
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 391 762 391
Size: 371 3279
Bme1580I G_kGCm'C
Cuts at: 378 437 1275 1368 2424 378
Size: 59 838 93 1056 1604
Fragments arranged by size:
1604 1056 838 93 59
BmgBI CAC'GTC
Cuts at: 560 560
Size: 3650
BmrI ACTGGGnnnn_n'
Cuts at: 862 943 1278 3422 862
Size: 81 335 2144 1090
Fragments arranged by size:
2144 1090 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 2017 246
Size: 1771 1879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 390
Size: 3650
Bpu10I CC'TnA_GC
Cuts at: 124 313 469 124
Size: 189 156 3305
Fragments arranged by size:
3305 189 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 519 826 1705 2197 2495 397
Size: 122 307 879 492 298 1552
Fragments arranged by size:
1552 879 492 307 298 122
BsaI GGTCTCn'nnnn_
Cuts at: 254 3354 254
Size: 3100 550
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 292 478 1081 1329 2031 3527 292
Size: 186 603 248 702 1496 415
Fragments arranged by size:
1496 702 603 415 248 186
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 297 238
Size: 59 3591
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 220 232 664 1442 1632 2424 220
Size: 12 432 778 190 792 1446
Fragments arranged by size:
1446 792 778 432 190 12
Bsp1286I G_dGCh'C
Cuts at: 220 232 310 378 437 664 1275 1368 1442
Size: 12 78 68 59 227 611 93 74
Cuts at: 1442 1632 1694 2424 220
Size: 190 62 730 1446
Fragments arranged by size:
1446 730 611 227 190 93 78 74
68 62 59 12
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 330 700 914 1216 1597 330
Size: 370 214 302 381 2383
Fragments arranged by size:
2383 381 370 302 214
BsrBI CCG'CTC
Cuts at: 50 317 372 1942 1996 3480 3645 50
Size: 267 55 1570 54 1484 165 55
Fragments arranged by size:
1570 1484 267 165 55 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 115 1648 1829 2038 115
Size: 1533 181 209 1727
Fragments arranged by size:
1727 1533 209 181
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 187 2011 187
Size: 1824 1826
BstEII G'GTnAC_C
Cuts at: 609 609
Size: 3650
BstYI r'GATC_y
Cuts at: 1 506 675 1142 1165 1500 1746 2758 2844
Size: 505 169 467 23 335 246 1012 86
Cuts at: 2844 2856 3202 1
Size: 12 346 449
Fragments arranged by size:
1012 505 467 449 346 335 246 169
86 23 12
BtgI C'CryG_G
Cuts at: 264 264
Size: 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 474 1600 1781 1944 143
Size: 331 1126 181 163 1849
Fragments arranged by size:
1849 1126 331 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 575 1235 1409 1800 1827 3172 575
Size: 660 174 391 27 1345 1053
Fragments arranged by size:
1345 1053 660 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 298 1673 1883 298
Size: 1375 210 2065
Fragments arranged by size:
2065 1375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 230 662 230
Size: 432 3218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1475 1907 2654 2922 390
Size: 1085 432 747 268 1118
Fragments arranged by size:
1118 1085 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 145 145
Size: 3650
EcoO109I rG'GnC_Cy
Cuts at: 138 789 138
Size: 651 2999
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 295 1084 1332 2354 3333 295
Size: 789 248 1022 979 612
Fragments arranged by size:
1022 979 789 612 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 1492 1522 1524 1554 232
Size: 32 1228 30 2 30 2328
Fragments arranged by size:
2328 1228 32 30 30 2
HincII GTy'rAC
Cuts at: 552 689 552
Size: 137 3513
KasI G'GCGC_C
Cuts at: 291 1080 1328 291
Size: 789 248 2613
Fragments arranged by size:
2613 789 248
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 233 257 2321 2505 140
Size: 93 24 2064 184 1285
Fragments arranged by size:
2064 1285 184 93 24
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 385 1639 1766 385
Size: 1254 127 2269
Fragments arranged by size:
2269 1254 127
MspA1I CmG'CkG
Cuts at: 266 761 1075 1435 2448 2693 266
Size: 495 314 360 1013 245 1223
Fragments arranged by size:
1223 1013 495 360 314 245
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 292 1081 1329 292
Size: 789 248 2613
Fragments arranged by size:
2613 789 248
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 384 703 1203 2110 384
Size: 319 500 907 1924
Fragments arranged by size:
1924 907 500 319
PasI CC'CwG_GG
Cuts at: 428 428
Size: 3650
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PpuMI rG'GwC_Cy
Cuts at: 138 138
Size: 3650
PspOMI G'GGCC_C
Cuts at: 374 374
Size: 3650
PstI C_TGCA'G
Cuts at: 697 1382 697
Size: 685 2965
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 232 664 232
Size: 432 3218
SacII CC_GC'GG
Cuts at: 267 267
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 298 1673 1883 298
Size: 1375 210 2065
Fragments arranged by size:
2065 1375 210
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 693 1378 2371 2562 33
Size: 660 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 269 269
Size: 3650
SfoI GGC'GCC
Cuts at: 293 1082 1330 293
Size: 789 248 2613
Fragments arranged by size:
2613 789 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 412 498 805 1720 2212 2474 412
Size: 86 307 915 492 262 1588
Fragments arranged by size:
1588 915 492 307 262 86
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 384 703 1203 384
Size: 319 500 2831
Fragments arranged by size:
2831 500 319
StuI AGG'CCT
Cuts at: 400 400
Size: 3650
StyI C'CwwG_G
Cuts at: 784 1138 784
Size: 354 3296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 1233 1366 1825 2034 420
Size: 813 133 459 209 2036
Fragments arranged by size:
2036 813 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI