Restriction map for
pF1KB8880
(Circular) (Six-base) MAPSORT of: pF1KB8880.seq Check: 7026 from: 1 to: 3638
pF1KB8880 3638 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 299 676 299
Size: 377 3261
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 640 1463 1895 2642 2910 283
Size: 357 823 432 747 268 1011
Fragments arranged by size:
1011 823 747 432 357 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 121 642 2991 3247 121
Size: 521 2349 256 512
Fragments arranged by size:
2349 521 512 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 652 1682 652
Size: 1030 2608
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BbsI GAAGACnn'nnnn_
Cuts at: 353 353
Size: 3638
BciVI GTATCCnnnnn_n'
Cuts at: 438 1536 2297 3638 438
Size: 1098 761 1341 438
Fragments arranged by size:
1341 1098 761 438
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 596 1153 3190 596
Size: 557 2037 1044
Fragments arranged by size:
2037 1044 557
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 372 850 931 1266 3410 372
Size: 478 81 335 2144 600
Fragments arranged by size:
2144 600 478 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
Bpu10I CC'TnA_GC
Cuts at: 146 146
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 3638
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 524 1063 1821 2398 2894 524
Size: 539 758 577 496 1268
Fragments arranged by size:
1268 758 577 539 496
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 652 1263 1356 1430 1620 1682 2412 652
Size: 611 93 74 190 62 730 1878
Fragments arranged by size:
1878 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstYI r'GATC_y
Cuts at: 1 596 663 1130 1153 1488 1734 2746 2832
Size: 595 67 467 23 335 246 1012 86
Cuts at: 2832 2844 3190 1
Size: 12 346 449
Fragments arranged by size:
1012 595 467 449 346 335 246 86
67 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 166 302 881 166
Size: 136 579 2923
Fragments arranged by size:
2923 579 136
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 1223 1397 1788 1815 3160 1223
Size: 174 391 27 1345 1701
Fragments arranged by size:
1701 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 640 1463 1895 2642 2910 283
Size: 357 823 432 747 268 1011
Fragments arranged by size:
1011 823 747 432 357 268
EcoNI CCTnn'n_nnAGG
Cuts at: 188 188
Size: 3638
EcoO109I rG'GnC_Cy
Cuts at: 777 777
Size: 3638
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 288 1657 288
Size: 1369 2269
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1480 1510 1512 1542 1480
Size: 30 2 30 3576
Fragments arranged by size:
3576 30 30 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 1627 1754 1627
Size: 127 3511
MspA1I CmG'CkG
Cuts at: 749 1063 1423 2436 2681 749
Size: 314 360 1013 245 1706
Fragments arranged by size:
1706 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PflMI CCAn_nnn'nTGG
Cuts at: 184 330 184
Size: 146 3492
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PstI C_TGCA'G
Cuts at: 161 685 1370 161
Size: 524 685 2429
Fragments arranged by size:
2429 685 524
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 1063 1423 1063
Size: 360 3278
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 157 169 681 1366 2359 2550 33
Size: 124 12 512 685 993 191 1121
Fragments arranged by size:
1121 993 685 512 191 124 12
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 772 1126 772
Size: 354 3284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 1354 1813 2022 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 579 669 579
Size: 90 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
XmnI GAAnn'nnTTC
Cuts at: 398 398
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI