Restriction map for
pF1KB8879
(Circular) (Six-base) MAPSORT of: pF1KB8879.seq Check: 6584 from: 1 to: 3632
pF1KB8879 3632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1889 2636 2904 1457
Size: 432 747 268 2185
Fragments arranged by size:
2185 747 432 268
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AleI CACnn'nnGTG
Cuts at: 554 554
Size: 3632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 543 543
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 199 937 2504 199
Size: 738 1567 1327
Fragments arranged by size:
1567 1327 738
ApaLI G'TGCA_C
Cuts at: 518 2402 518
Size: 1884 1748
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 444 531 652 444
Size: 87 121 3424
Fragments arranged by size:
3424 121 87
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 177 345 648 1062 1310 1345 177
Size: 168 303 414 248 35 2464
Fragments arranged by size:
2464 414 303 248 168 35
BanII G_rGCy'C
Cuts at: 209 483 646 1676 209
Size: 274 163 1030 2165
Fragments arranged by size:
2165 1030 274 163
BbeI G_GCGC'C
Cuts at: 349 1066 1314 349
Size: 717 248 2667
Fragments arranged by size:
2667 717 248
BbsI GAAGACnn'nnnn_
Cuts at: 423 423
Size: 3632
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglI GCCn_nnn'nGGC
Cuts at: 218 358 449 218
Size: 140 91 3401
Fragments arranged by size:
3401 140 91
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 744 744
Size: 3632
Bme1580I G_kGCm'C
Cuts at: 522 1257 1350 2406 522
Size: 735 93 1056 1748
Fragments arranged by size:
1748 1056 735 93
BmgBI CAC'GTC
Cuts at: 589 589
Size: 3632
BmrI ACTGGGnnnn_n'
Cuts at: 128 844 925 1260 3404 128
Size: 716 81 335 2144 356
Fragments arranged by size:
2144 716 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3336 3336
Size: 3632
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 346 1063 1311 2013 3509 346
Size: 717 248 702 1496 469
Fragments arranged by size:
1496 717 702 469 248
BsaWI w'CCGG_w
Cuts at: 257 821 1342 2020 2294 2441 2852 257
Size: 564 521 678 274 147 411 1037
Fragments arranged by size:
1037 678 564 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 375 405 375
Size: 30 3602
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 396 396
Size: 3632
BseYI C'CCAG_C
Cuts at: 1057 1815 2392 2888 1057
Size: 758 577 496 1801
Fragments arranged by size:
1801 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 548
Size: 3632
BsiEI CG_ry'CG
Cuts at: 104 1174 1220 2428 104
Size: 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 46
BsiHKAI G_wGCw'C
Cuts at: 209 522 646 1424 1614 2406 209
Size: 313 124 778 190 792 1435
Fragments arranged by size:
1435 792 778 313 190 124
Bsp1286I G_dGCh'C
Cuts at: 209 483 522 646 1257 1350 1424 1614 1676
Size: 274 39 124 611 93 74 190 62
Cuts at: 1676 2406 209
Size: 730 1435
Fragments arranged by size:
1435 730 611 274 190 124 93 74
62 39
BspEI T'CCGG_A
Cuts at: 257 2852 257
Size: 2595 1037
BspHI T'CATG_A
Cuts at: 426 2808 426
Size: 2382 1250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 682 896 1198 1579 230
Size: 452 214 302 381 2283
Fragments arranged by size:
2283 452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1924 1978 3462 3627 50
Size: 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 1630 1811 2020 1630
Size: 181 209 3242
Fragments arranged by size:
3242 209 181
BssHII G'CGCG_C
Cuts at: 267 267
Size: 3632
BssSI C'ACGA_G
Cuts at: 364 1903 2261 364
Size: 1539 358 1735
Fragments arranged by size:
1735 1539 358
BstAPI GCAn_nnn'nTGC
Cuts at: 199 1009 199
Size: 810 2822
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstEII G'GTnAC_C
Cuts at: 249 249
Size: 3632
BstYI r'GATC_y
Cuts at: 1 657 1124 1147 1482 1728 2740 2826 2838
Size: 656 467 23 335 246 1012 86 12
Cuts at: 2838 3184 1
Size: 346 449
Fragments arranged by size:
1012 656 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 313 469 615 107
Size: 206 156 146 3124
Fragments arranged by size:
3124 206 156 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 609 1582 1763 1926 609
Size: 973 181 163 2315
Fragments arranged by size:
2315 973 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 1217 1391 1782 1809 3154 1217
Size: 174 391 27 1345 1695
Fragments arranged by size:
1695 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 467 1840 2150 2296 3442 467
Size: 1373 310 146 1146 657
Fragments arranged by size:
1373 1146 657 310 146
EcoICRI GAG'CTC
Cuts at: 207 644 207
Size: 437 3195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 1457 1889 2636 2904 236
Size: 1221 432 747 268 964
Fragments arranged by size:
1221 964 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 130 771 130
Size: 641 2991
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 238 1413 238
Size: 1175 2457
HaeII r_GCGC'y
Cuts at: 165 340 349 444 1066 1314 2336 3315 165
Size: 175 9 95 622 248 1022 979 482
Fragments arranged by size:
1022 979 622 482 248 175 95 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 1474 1504 1506 1536 375
Size: 32 1067 30 2 30 2471
Fragments arranged by size:
2471 1067 32 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 345 1062 1310 345
Size: 717 248 2667
Fragments arranged by size:
2667 717 248
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2487 2303
Size: 184 3448
MscI TGG'CCA
Cuts at: 1393 1393
Size: 3632
MslI CAynn'nnrTG
Cuts at: 431 554 1621 1748 431
Size: 123 1067 127 2315
Fragments arranged by size:
2315 1067 127 123
MspA1I CmG'CkG
Cuts at: 196 315 355 743 1057 1417 2430 2675 196
Size: 119 40 388 314 360 1013 245 1153
Fragments arranged by size:
1153 1013 388 360 314 245 119 40
NaeI GCC'GGC
Cuts at: 1813 1813
Size: 3632
NarI GG'CG_CC
Cuts at: 346 1063 1311 346
Size: 717 248 2667
Fragments arranged by size:
2667 717 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 1811 1811
Size: 3632
NspI r_CATG'y
Cuts at: 685 1185 2092 685
Size: 500 907 2225
Fragments arranged by size:
2225 907 500
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PfoI T'CCnGG_A
Cuts at: 543 543
Size: 3632
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PpuMI rG'GwC_Cy
Cuts at: 130 130
Size: 3632
PstI C_TGCA'G
Cuts at: 143 679 1364 143
Size: 536 685 2411
Fragments arranged by size:
2411 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 209 646 209
Size: 437 3195
SacII CC_GC'GG
Cuts at: 316 316
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SanDI GG'GwC_CC
Cuts at: 130 130
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SexAI A'CCwGG_T
Cuts at: 420 420
Size: 3632
SfcI C'TryA_G
Cuts at: 33 139 675 1360 2353 2544 33
Size: 106 536 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 191 106
SfoI GGC'GCC
Cuts at: 347 1064 1312 347
Size: 717 248 2667
Fragments arranged by size:
2667 717 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 533 654 533
Size: 121 3511
SmlI C'TyrA_G
Cuts at: 787 1702 2194 2456 787
Size: 915 492 262 1963
Fragments arranged by size:
1963 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StyI C'CwwG_G
Cuts at: 107 766 1120 107
Size: 659 354 2619
Fragments arranged by size:
2619 659 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1215 1348 1807 2016 1215
Size: 133 459 209 2831
Fragments arranged by size:
2831 459 209 133
TatI w'GTAC_w
Cuts at: 3147 3147
Size: 3632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3600
Tth111I GACn'n_nGTC
Cuts at: 422 1429 422
Size: 1007 2625
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1127 1127
Size: 3632
XmaI C'CCGG_G
Cuts at: 531 652 531
Size: 121 3511
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI