Restriction map for
pF1KB8877
(Circular) (Six-base) MAPSORT of: pF1KB8877.seq Check: 4012 from: 1 to: 3617
pF1KB8877 3617 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1874 2621 2889 1442
Size: 432 747 268 2170
Fragments arranged by size:
2170 747 432 268
AflIII A'CryG_T
Cuts at: 969 2073 3050 969
Size: 1104 977 1536
Fragments arranged by size:
1536 1104 977
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 452 452
Size: 3617
AlwNI CAG_nnn'CTG
Cuts at: 922 2489 922
Size: 1567 2050
ApaLI G'TGCA_C
Cuts at: 2387 2387
Size: 3617
ApoI r'AATT_y
Cuts at: 621 2970 3226 621
Size: 2349 256 1012
Fragments arranged by size:
2349 1012 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 256 637 256
Size: 381 3236
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 633 1047 1295 1330 633
Size: 414 248 35 2920
Fragments arranged by size:
2920 414 248 35
BanII G_rGCy'C
Cuts at: 631 1661 631
Size: 1030 2587
BbeI G_GCGC'C
Cuts at: 1051 1299 1051
Size: 248 3369
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2276 3617 1515
Size: 761 1341 1515
Fragments arranged by size:
1515 1341 761
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 729 729
Size: 3617
Bme1580I G_kGCm'C
Cuts at: 1242 1335 2391 1242
Size: 93 1056 2468
Fragments arranged by size:
2468 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 829 910 1245 3389 829
Size: 81 335 2144 1057
Fragments arranged by size:
2144 1057 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 3617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1672 2164 2462 793
Size: 879 492 298 1948
Fragments arranged by size:
1948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 136 1048 1296 1998 3494 136
Size: 912 248 702 1496 259
Fragments arranged by size:
1496 912 702 259 248
BsaWI w'CCGG_w
Cuts at: 806 1327 2005 2279 2426 2837 806
Size: 521 678 274 147 411 1586
Fragments arranged by size:
1586 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 589 1042 1800 2377 2873 589
Size: 453 758 577 496 1333
Fragments arranged by size:
1333 758 577 496 453
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 631 1409 1599 2391 631
Size: 778 190 792 1857
Fragments arranged by size:
1857 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 631 1242 1335 1409 1599 1661 2391 631
Size: 611 93 74 190 62 730 1857
Fragments arranged by size:
1857 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2837 2837
Size: 3617
BspHI T'CATG_A
Cuts at: 330 2793 330
Size: 2463 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 225 337 428 667 881 1183 1564 225
Size: 112 91 239 214 302 381 2278
Fragments arranged by size:
2278 381 302 239 214 112 91
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 498 1529 498
Size: 1031 2586
BsrFI r'CCGG_y
Cuts at: 1615 1796 2005 1615
Size: 181 209 3227
Fragments arranged by size:
3227 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstXI CCAn_nnnn'nTGG
Cuts at: 495 495
Size: 3617
BstYI r'GATC_y
Cuts at: 1 642 1109 1132 1467 1713 2725 2811 2823
Size: 641 467 23 335 246 1012 86 12
Cuts at: 2823 3169 1
Size: 346 449
Fragments arranged by size:
1012 641 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 860 860
Size: 3617
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 110 338 485 536 1202 1376 1767 1794 3139
Size: 228 147 51 666 174 391 27 1345
Cuts at: 3139 110
Size: 588
Fragments arranged by size:
1345 666 588 391 228 174 147 51
27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2135 2281 3427 1825
Size: 310 146 1146 2015
Fragments arranged by size:
2015 1146 310 146
EcoICRI GAG'CTC
Cuts at: 629 629
Size: 3617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1442 1874 2621 2889 397
Size: 1045 432 747 268 1125
Fragments arranged by size:
1125 1045 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 574 574
Size: 3617
EcoO109I rG'GnC_Cy
Cuts at: 156 756 156
Size: 600 3017
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3617
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 1051 1299 2321 3300 1051
Size: 248 1022 979 1368
Fragments arranged by size:
1368 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 1459 1489 1491 1521 121
Size: 32 1306 30 2 30 2217
Fragments arranged by size:
2217 1306 32 30 30 2
HincII GTy'rAC
Cuts at: 396 656 396
Size: 260 3357
HpaI GTT'AAC
Cuts at: 396 396
Size: 3617
KasI G'GCGC_C
Cuts at: 1047 1295 1047
Size: 248 3369
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2472 2288
Size: 184 3433
MscI TGG'CCA
Cuts at: 538 1378 538
Size: 840 2777
MslI CAynn'nnrTG
Cuts at: 1606 1733 1606
Size: 127 3490
MspA1I CmG'CkG
Cuts at: 145 728 1042 1402 2415 2660 145
Size: 583 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 583 360 314 245
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 1048 1296 1048
Size: 248 3369
NcoI C'CATG_G
Cuts at: 107 107
Size: 3617
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 3617
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 3585
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 3617
PstI C_TGCA'G
Cuts at: 236 664 1349 236
Size: 428 685 2504
Fragments arranged by size:
2504 685 428
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 145 1042 1402 145
Size: 897 360 2360
Fragments arranged by size:
2360 897 360
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 631 631
Size: 3617
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 3134 3134
Size: 3617
SfcI C'TryA_G
Cuts at: 33 232 660 1345 2338 2529 33
Size: 199 428 685 993 191 1121
Fragments arranged by size:
1121 993 685 428 199 191
SfoI GGC'GCC
Cuts at: 1049 1297 1049
Size: 248 3369
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 772 1687 2179 2441 772
Size: 915 492 262 1948
Fragments arranged by size:
1948 915 492 262
SpeI A'CTAG_T
Cuts at: 67 172 67
Size: 105 3512
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StyI C'CwwG_G
Cuts at: 107 118 751 1105 107
Size: 11 633 354 2619
Fragments arranged by size:
2619 633 354 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 323 1200 1333 1792 2001 323
Size: 877 133 459 209 1939
Fragments arranged by size:
1939 877 459 209 133
TatI w'GTAC_w
Cuts at: 3132 3132
Size: 3617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3585
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1112 1112
Size: 3617
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI