Restriction map for
pF1KB8875
(Circular) (Six-base) MAPSORT of: pF1KB8875.seq Check: 7063 from: 1 to: 3611
pF1KB8875 3611 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 328 627 328
Size: 299 3312
AclI AA'CG_TT
Cuts at: 3451 3451
Size: 3611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 397 1436 1868 2615 2883 310
Size: 87 1039 432 747 268 1038
Fragments arranged by size:
1039 1038 747 432 268 87
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AleI CACnn'nnGTG
Cuts at: 469 469
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 615 2964 3220 615
Size: 2349 256 1006
Fragments arranged by size:
2349 1006 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 158 631 158
Size: 473 3138
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 298 328 421 627 1041 1289 1324 298
Size: 30 93 206 414 248 35 2585
Fragments arranged by size:
2585 414 248 206 93 35 30
BanII G_rGCy'C
Cuts at: 561 625 1655 561
Size: 64 1030 2517
Fragments arranged by size:
2517 1030 64
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 255 289 90
Size: 34 131 34 3412
Fragments arranged by size:
3412 131 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 723 723
Size: 3611
Bme1580I G_kGCm'C
Cuts at: 432 1236 1329 2385 432
Size: 804 93 1056 1658
Fragments arranged by size:
1658 1056 804 93
BmgBI CAC'GTC
Cuts at: 168 168
Size: 3611
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 1978 575
Size: 1403 2208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 301 136
Size: 165 3446
Bpu10I CC'TnA_GC
Cuts at: 560 560
Size: 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 529 3315 529
Size: 2786 825
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 503 1125 3162 6
Size: 497 622 2037 455
Fragments arranged by size:
2037 622 497 455
BsaHI Gr'CG_yC
Cuts at: 215 515 1042 1290 1992 3488 215
Size: 300 527 248 702 1496 338
Fragments arranged by size:
1496 702 527 338 300 248
BsaWI w'CCGG_w
Cuts at: 325 800 1321 1999 2273 2420 2831 325
Size: 475 521 678 274 147 411 1105
Fragments arranged by size:
1105 678 521 475 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 3611
BseYI C'CCAG_C
Cuts at: 1036 1794 2371 2867 1036
Size: 758 577 496 1780
Fragments arranged by size:
1780 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 190 175
Size: 15 3596
BsiEI CG_ry'CG
Cuts at: 104 138 1153 1199 2407 104
Size: 34 1015 46 1208 1308
Fragments arranged by size:
1308 1208 1015 46 34
BsiHKAI G_wGCw'C
Cuts at: 395 625 1403 1593 2385 395
Size: 230 778 190 792 1621
Fragments arranged by size:
1621 792 778 230 190
Bsp1286I G_dGCh'C
Cuts at: 395 432 561 625 1236 1329 1403 1593 1655
Size: 37 129 64 611 93 74 190 62
Cuts at: 1655 2385 395
Size: 730 1621
Fragments arranged by size:
1621 730 611 190 129 93 74 64
62 37
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 251 2787 251
Size: 2536 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 661 875 1177 1558 237
Size: 424 214 302 381 2290
Fragments arranged by size:
2290 424 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 130 137 1609 1790 1999 130
Size: 7 1472 181 209 1742
Fragments arranged by size:
1742 1472 209 181 7
BssSI C'ACGA_G
Cuts at: 568 1882 2240 568
Size: 1314 358 1939
Fragments arranged by size:
1939 1314 358
BstAPI GCAn_nnn'nTGC
Cuts at: 177 988 177
Size: 811 2800
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstXI CCAn_nnnn'nTGG
Cuts at: 114 279 114
Size: 165 3446
BstYI r'GATC_y
Cuts at: 1 636 1103 1126 1461 1707 2719 2805 2817
Size: 635 467 23 335 246 1012 86 12
Cuts at: 2817 3163 1
Size: 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 258 490 1561 1742 1905 258
Size: 232 1071 181 163 1964
Fragments arranged by size:
1964 1071 232 181 163
BtsI GCAGTG_nn'
Cuts at: 464 544 854 464
Size: 80 310 3221
Fragments arranged by size:
3221 310 80
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DraIII CAC_nnn'GTG
Cuts at: 594 594
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 1317 2175 1317
Size: 858 2753
EaeI y'GGCC_r
Cuts at: 1196 1370 1761 1788 3133 1196
Size: 174 391 27 1345 1674
Fragments arranged by size:
1674 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1819 2129 2275 3421 1819
Size: 310 146 1146 2009
Fragments arranged by size:
2009 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 301 310 397 1436 1868 2615 2883 136
Size: 165 9 87 1039 432 747 268 864
Fragments arranged by size:
1039 864 747 432 268 165 87 9
EcoO109I rG'GnC_Cy
Cuts at: 381 564 750 381
Size: 183 186 3242
Fragments arranged by size:
3242 186 183
EcoRI G'AATT_C
Cuts at: 615 615
Size: 3611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1630 1630
Size: 3611
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 244 1045 1293 2315 3294 244
Size: 801 248 1022 979 561
Fragments arranged by size:
1022 979 801 561 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1453 1483 1485 1515 1453
Size: 30 2 30 3549
Fragments arranged by size:
3549 30 30 2
HincII GTy'rAC
Cuts at: 408 650 408
Size: 242 3369
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 332 631 332
Size: 299 3312
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2466 2282
Size: 184 3427
MscI TGG'CCA
Cuts at: 1372 1372
Size: 3611
MslI CAynn'nnrTG
Cuts at: 469 1600 1727 469
Size: 1131 127 2353
Fragments arranged by size:
2353 1131 127
MspA1I CmG'CkG
Cuts at: 490 603 722 1036 1396 2409 2654 490
Size: 113 119 314 360 1013 245 1447
Fragments arranged by size:
1447 1013 360 314 245 119 113
NaeI GCC'GGC
Cuts at: 132 1792 132
Size: 1660 1951
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NgoMIV G'CCGG_C
Cuts at: 130 1790 130
Size: 1660 1951
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PflMI CCAn_nnn'nTGG
Cuts at: 594 594
Size: 3611
PfoI T'CCnGG_A
Cuts at: 314 365 314
Size: 51 3560
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PpuMI rG'GwC_Cy
Cuts at: 381 564 381
Size: 183 3428
PstI C_TGCA'G
Cuts at: 248 551 584 658 1343 248
Size: 303 33 74 685 2516
Fragments arranged by size:
2516 685 303 74 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 490 1036 1396 490
Size: 546 360 2705
Fragments arranged by size:
2705 546 360
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 584 658 584
Size: 74 3537
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SfcI C'TryA_G
Cuts at: 33 244 547 580 654 1339 2332 2523 33
Size: 211 303 33 74 685 993 191 1121
Fragments arranged by size:
1121 993 685 303 211 191 74 33
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SgrAI Cr'CCGG_yG
Cuts at: 130 130
Size: 3611
SmaI CCC'GGG
Cuts at: 160 633 160
Size: 473 3138
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StyI C'CwwG_G
Cuts at: 533 745 1099 533
Size: 212 354 3045
Fragments arranged by size:
3045 354 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1194 1327 1786 1995 1194
Size: 133 459 209 2810
Fragments arranged by size:
2810 459 209 133
TatI w'GTAC_w
Cuts at: 3126 3126
Size: 3611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 3128 3160 422
Size: 32 2674 32 873
Fragments arranged by size:
2674 873 32 32
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 1106
Size: 3611
XmaI C'CCGG_G
Cuts at: 158 631 158
Size: 473 3138
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI