Restriction map for
pF1KB8873
(Circular) (Six-base) MAPSORT of: pF1KB8873.seq Check: 6028 from: 1 to: 3599
pF1KB8873 3599 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 236 236
Size: 3599
AccI GT'mk_AC
Cuts at: 637 637
Size: 3599
Acc65I G'GTAC_C
Cuts at: 615 615
Size: 3599
AclI AA'CG_TT
Cuts at: 3439 3439
Size: 3599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1424 1856 2603 2871 283
Size: 1141 432 747 268 1011
Fragments arranged by size:
1141 1011 747 432 268
AflIII A'CryG_T
Cuts at: 951 2055 3032 951
Size: 1104 977 1518
Fragments arranged by size:
1518 1104 977
AgeI A'CCGG_T
Cuts at: 1987 1987
Size: 3599
AlwNI CAG_nnn'CTG
Cuts at: 228 904 2471 228
Size: 676 1567 1356
Fragments arranged by size:
1567 1356 676
ApaI G_GGCC'C
Cuts at: 502 524 502
Size: 22 3577
ApaLI G'TGCA_C
Cuts at: 2369 2369
Size: 3599
ApoI r'AATT_y
Cuts at: 603 2952 3208 603
Size: 2349 256 994
Fragments arranged by size:
2349 994 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
AvaI C'yCGr_G
Cuts at: 494 619 494
Size: 125 3474
BamHI G'GATC_C
Cuts at: 624 624
Size: 3599
BanI G'GyrC_C
Cuts at: 169 486 615 1029 1277 1312 169
Size: 317 129 414 248 35 2456
Fragments arranged by size:
2456 414 317 248 129 35
BanII G_rGCy'C
Cuts at: 419 502 524 613 1643 419
Size: 83 22 89 1030 2375
Fragments arranged by size:
2375 1030 89 83 22
BbeI G_GCGC'C
Cuts at: 490 1033 1281 490
Size: 543 248 2808
Fragments arranged by size:
2808 543 248
BciVI GTATCCnnnnn_n'
Cuts at: 1497 2258 3599 1497
Size: 761 1341 1497
Fragments arranged by size:
1497 1341 761
BclI T'GATC_A
Cuts at: 1119 1119
Size: 3599
BglII A'GATC_T
Cuts at: 1114 3151 1114
Size: 2037 1562
BlpI GC'TnA_GC
Cuts at: 711 711
Size: 3599
Bme1580I G_kGCm'C
Cuts at: 502 524 1224 1317 2373 502
Size: 22 700 93 1056 1728
Fragments arranged by size:
1728 1056 700 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 811 892 1227 3371 811
Size: 81 335 2144 1039
Fragments arranged by size:
2144 1039 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 433 364
Size: 69 3530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 775 1654 2146 2444 268
Size: 507 879 492 298 1423
Fragments arranged by size:
1423 879 507 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3303 3303
Size: 3599
BsaAI yAC'GTr
Cuts at: 952 952
Size: 3599
BsaBI GATnn'nnATC
Cuts at: 6 1113 3150 6
Size: 1107 2037 455
Fragments arranged by size:
2037 1107 455
BsaHI Gr'CG_yC
Cuts at: 487 1030 1278 1980 3476 487
Size: 543 248 702 1496 610
Fragments arranged by size:
1496 702 610 543 248
BsaWI w'CCGG_w
Cuts at: 788 1309 1987 2261 2408 2819 788
Size: 521 678 274 147 411 1568
Fragments arranged by size:
1568 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 262 352 202
Size: 60 90 3449
Fragments arranged by size:
3449 90 60
BseYI C'CCAG_C
Cuts at: 1024 1782 2359 2855 1024
Size: 758 577 496 1768
Fragments arranged by size:
1768 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 592 1141 1187 2395 104
Size: 488 549 46 1208 1308
Fragments arranged by size:
1308 1208 549 488 46
BsiHKAI G_wGCw'C
Cuts at: 419 613 1391 1581 2373 419
Size: 194 778 190 792 1645
Fragments arranged by size:
1645 792 778 194 190
Bsp1286I G_dGCh'C
Cuts at: 419 502 524 613 1224 1317 1391 1581 1643
Size: 83 22 89 611 93 74 190 62
Cuts at: 1643 2373 419
Size: 730 1645
Fragments arranged by size:
1645 730 611 190 93 89 83 74
62 22
BspEI T'CCGG_A
Cuts at: 2819 2819
Size: 3599
BspHI T'CATG_A
Cuts at: 2775 2775
Size: 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 649 863 1165 1546 236
Size: 413 214 302 381 2289
Fragments arranged by size:
2289 413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1891 1945 3429 3594 50
Size: 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1511 1511
Size: 3599
BsrFI r'CCGG_y
Cuts at: 272 511 1597 1778 1987 272
Size: 239 1086 181 209 1884
Fragments arranged by size:
1884 1086 239 209 181
BssSI C'ACGA_G
Cuts at: 1870 2228 1870
Size: 358 3241
BstAPI GCAn_nnn'nTGC
Cuts at: 976 976
Size: 3599
BstBI TT'CG_AA
Cuts at: 1960 1960
Size: 3599
BstEII G'GTnAC_C
Cuts at: 195 195
Size: 3599
BstYI r'GATC_y
Cuts at: 1 624 1091 1114 1449 1695 2707 2793 2805
Size: 623 467 23 335 246 1012 86 12
Cuts at: 2805 3151 1
Size: 346 449
Fragments arranged by size:
1012 623 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 291 482 291
Size: 191 3408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1549 1730 1893 1549
Size: 181 163 3255
Fragments arranged by size:
3255 181 163
BtsI GCAGTG_nn'
Cuts at: 842 842
Size: 3599
DraI TTT'AAA
Cuts at: 598 598
Size: 3599
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2163 1305
Size: 858 2741
EaeI y'GGCC_r
Cuts at: 542 1184 1358 1749 1776 3121 542
Size: 642 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 642 391 174 27
EagI C'GGCC_G
Cuts at: 1184 1184
Size: 3599
EarI CTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 1807 2117 2263 3409 373
Size: 1434 310 146 1146 563
Fragments arranged by size:
1434 1146 563 310 146
EcoICRI GAG'CTC
Cuts at: 417 611 417
Size: 194 3405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 364 433 1424 1856 2603 2871 283
Size: 81 69 991 432 747 268 1011
Fragments arranged by size:
1011 991 747 432 268 81 69
EcoNI CCTnn'n_nnAGG
Cuts at: 241 241
Size: 3599
EcoO109I rG'GnC_Cy
Cuts at: 234 498 499 520 521 738 234
Size: 264 1 21 1 217 3095
Fragments arranged by size:
3095 264 217 21 1 1
EcoRI G'AATT_C
Cuts at: 603 603
Size: 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1618 1618
Size: 3599
FspI TGC'GCA
Cuts at: 1380 1380
Size: 3599
HaeII r_GCGC'y
Cuts at: 273 448 490 1033 1281 2303 3282 273
Size: 175 42 543 248 1022 979 590
Fragments arranged by size:
1022 979 590 543 248 175 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1441 1471 1473 1503 574
Size: 32 835 30 2 30 2670
Fragments arranged by size:
2670 835 32 30 30 2
HincII GTy'rAC
Cuts at: 638 638
Size: 3599
KasI G'GCGC_C
Cuts at: 486 1029 1277 486
Size: 543 248 2808
Fragments arranged by size:
2808 543 248
KpnI G_GTAC'C
Cuts at: 619 619
Size: 3599
MluI A'CGCG_T
Cuts at: 3032 3032
Size: 3599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2454 2270
Size: 184 3415
MscI TGG'CCA
Cuts at: 1360 1360
Size: 3599
MslI CAynn'nnrTG
Cuts at: 205 1588 1715 205
Size: 1383 127 2089
Fragments arranged by size:
2089 1383 127
MspA1I CmG'CkG
Cuts at: 261 293 308 710 1024 1384 2397 2642 261
Size: 32 15 402 314 360 1013 245 1218
Fragments arranged by size:
1218 1013 402 360 314 245 32 15
NaeI GCC'GGC
Cuts at: 274 513 1780 274
Size: 239 1267 2093
Fragments arranged by size:
2093 1267 239
NarI GG'CG_CC
Cuts at: 487 1030 1278 487
Size: 543 248 2808
Fragments arranged by size:
2808 543 248
NgoMIV G'CCGG_C
Cuts at: 272 511 1778 272
Size: 239 1267 2093
Fragments arranged by size:
2093 1267 239
NspI r_CATG'y
Cuts at: 652 1152 2059 652
Size: 500 907 2192
Fragments arranged by size:
2192 907 500
PciI A'CATG_T
Cuts at: 2055 2055
Size: 3599
PmeI GTTT'AAAC
Cuts at: 598 598
Size: 3599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 3567
PspOMI G'GGCC_C
Cuts at: 498 520 498
Size: 22 3577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 280 312 280
Size: 32 3567
PstI C_TGCA'G
Cuts at: 368 452 646 1331 368
Size: 84 194 685 2636
Fragments arranged by size:
2636 685 194 84
PvuI CG_AT'CG
Cuts at: 104 592 104
Size: 488 3111
PvuII CAG'CTG
Cuts at: 261 308 1024 1384 261
Size: 47 716 360 2476
Fragments arranged by size:
2476 716 360 47
RsrII CG'GwC_CG
Cuts at: 1794 1794
Size: 3599
SacI G_AGCT'C
Cuts at: 419 613 419
Size: 194 3405
SacII CC_GC'GG
Cuts at: 294 294
Size: 3599
SalI G'TCGA_C
Cuts at: 636 636
Size: 3599
SapI GCTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
SbfI CC_TGCA'GG
Cuts at: 646 646
Size: 3599
ScaI AGT'ACT
Cuts at: 3116 3116
Size: 3599
SfcI C'TryA_G
Cuts at: 33 364 448 642 1327 2320 2511 33
Size: 331 84 194 685 993 191 1121
Fragments arranged by size:
1121 993 685 331 194 191 84
SfoI GGC'GCC
Cuts at: 488 1031 1279 488
Size: 543 248 2808
Fragments arranged by size:
2808 543 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
SgrAI Cr'CCGG_yG
Cuts at: 272 272
Size: 3599
SmaI CCC'GGG
Cuts at: 496 621 496
Size: 125 3474
SmlI C'TyrA_G
Cuts at: 283 754 1669 2161 2423 283
Size: 471 915 492 262 1459
Fragments arranged by size:
1459 915 492 471 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3599
SphI G_CATG'C
Cuts at: 652 1152 652
Size: 500 3099
StuI AGG'CCT
Cuts at: 136 136
Size: 3599
StyI C'CwwG_G
Cuts at: 242 733 1087 242
Size: 491 354 2754
Fragments arranged by size:
2754 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1182 1315 1774 1983 1182
Size: 133 459 209 2798
Fragments arranged by size:
2798 459 209 133
TatI w'GTAC_w
Cuts at: 3114 3114
Size: 3599
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3567
Tth111I GACn'n_nGTC
Cuts at: 201 1396 201
Size: 1195 2404
XbaI T'CTAG_A
Cuts at: 630 630
Size: 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 1094 408
Size: 686 2913
XmaI C'CCGG_G
Cuts at: 494 619 494
Size: 125 3474
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI