Restriction map for
pF1KB8868
(Circular) (Six-base) MAPSORT of: pF1KB8868.seq Check: 4176 from: 1 to: 3491
pF1KB8868 3491 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 529 529
Size: 3491
Acc65I G'GTAC_C
Cuts at: 507 507
Size: 3491
AclI AA'CG_TT
Cuts at: 3331 3331
Size: 3491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1748 2495 2763 1316
Size: 432 747 268 2044
Fragments arranged by size:
2044 747 432 268
AflIII A'CryG_T
Cuts at: 843 1947 2924 843
Size: 1104 977 1410
Fragments arranged by size:
1410 1104 977
AgeI A'CCGG_T
Cuts at: 308 1879 308
Size: 1571 1920
AhdI GACnn_n'nnGTC
Cuts at: 345 345
Size: 3491
AlwNI CAG_nnn'CTG
Cuts at: 126 796 2363 126
Size: 670 1567 1254
Fragments arranged by size:
1567 1254 670
ApaLI G'TGCA_C
Cuts at: 2261 2261
Size: 3491
ApoI r'AATT_y
Cuts at: 495 2844 3100 495
Size: 2349 256 886
Fragments arranged by size:
2349 886 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
AvaI C'yCGr_G
Cuts at: 181 277 511 181
Size: 96 234 3161
Fragments arranged by size:
3161 234 96
BamHI G'GATC_C
Cuts at: 516 516
Size: 3491
BanI G'GyrC_C
Cuts at: 186 289 507 921 1169 1204 186
Size: 103 218 414 248 35 2473
Fragments arranged by size:
2473 414 248 218 103 35
BanII G_rGCy'C
Cuts at: 366 505 1535 366
Size: 139 1030 2322
Fragments arranged by size:
2322 1030 139
BbeI G_GCGC'C
Cuts at: 190 293 925 1173 190
Size: 103 632 248 2508
Fragments arranged by size:
2508 632 248 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3457
BciVI GTATCCnnnnn_n'
Cuts at: 1389 2150 3491 1389
Size: 761 1341 1389
Fragments arranged by size:
1389 1341 761
BclI T'GATC_A
Cuts at: 1011 1011
Size: 3491
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3491
BglII A'GATC_T
Cuts at: 1006 3043 1006
Size: 2037 1454
BlpI GC'TnA_GC
Cuts at: 603 603
Size: 3491
Bme1580I G_kGCm'C
Cuts at: 1116 1209 2265 1116
Size: 93 1056 2342
Fragments arranged by size:
2342 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 703 784 1119 3263 703
Size: 81 335 2144 931
Fragments arranged by size:
2144 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 430 667 1546 2038 2336 283
Size: 147 237 879 492 298 1438
Fragments arranged by size:
1438 879 492 298 237 147
BsaI GGTCTCn'nnnn_
Cuts at: 3195 3195
Size: 3491
BsaAI yAC'GTr
Cuts at: 844 844
Size: 3491
BsaBI GATnn'nnATC
Cuts at: 6 1005 3042 6
Size: 999 2037 455
Fragments arranged by size:
2037 999 455
BsaHI Gr'CG_yC
Cuts at: 187 290 922 1170 1872 3368 187
Size: 103 632 248 702 1496 310
Fragments arranged by size:
1496 702 632 310 248 103
BsaWI w'CCGG_w
Cuts at: 308 680 1201 1879 2153 2300 2711 308
Size: 372 521 678 274 147 411 1088
Fragments arranged by size:
1088 678 521 411 372 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 235
Size: 3491
BseYI C'CCAG_C
Cuts at: 119 916 1674 2251 2747 119
Size: 797 758 577 496 863
Fragments arranged by size:
863 797 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 309 313 1033 1079 2287 104
Size: 205 4 720 46 1208 1308
Fragments arranged by size:
1308 1208 720 205 46 4
BsiHKAI G_wGCw'C
Cuts at: 383 505 1283 1473 2265 383
Size: 122 778 190 792 1609
Fragments arranged by size:
1609 792 778 190 122
Bsp1286I G_dGCh'C
Cuts at: 366 383 505 1116 1209 1283 1473 1535 2265
Size: 17 122 611 93 74 190 62 730
Cuts at: 2265 366
Size: 1592
Fragments arranged by size:
1592 730 611 190 122 93 74 62
17
BspEI T'CCGG_A
Cuts at: 2711 2711
Size: 3491
BspHI T'CATG_A
Cuts at: 2667 2667
Size: 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 338 541 755 1057 1438 338
Size: 203 214 302 381 2391
Fragments arranged by size:
2391 381 302 214 203
BsrBI CCG'CTC
Cuts at: 50 473 1783 1837 3321 3486 50
Size: 423 1310 54 1484 165 55
Fragments arranged by size:
1484 1310 423 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1403 1403
Size: 3491
BsrFI r'CCGG_y
Cuts at: 213 308 1489 1670 1879 213
Size: 95 1181 181 209 1825
Fragments arranged by size:
1825 1181 209 181 95
BssHII G'CGCG_C
Cuts at: 391 391
Size: 3491
BssSI C'ACGA_G
Cuts at: 1762 2120 1762
Size: 358 3133
BstAPI GCAn_nnn'nTGC
Cuts at: 868 868
Size: 3491
BstBI TT'CG_AA
Cuts at: 1852 1852
Size: 3491
BstYI r'GATC_y
Cuts at: 1 516 983 1006 1341 1587 2599 2685 2697
Size: 515 467 23 335 246 1012 86 12
Cuts at: 2697 3043 1
Size: 346 449
Fragments arranged by size:
1012 515 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1441 1622 1785 1441
Size: 181 163 3147
Fragments arranged by size:
3147 181 163
BtsI GCAGTG_nn'
Cuts at: 734 734
Size: 3491
DraI TTT'AAA
Cuts at: 490 490
Size: 3491
DrdI GACnn_nn'nnGTC
Cuts at: 1197 2055 1197
Size: 858 2633
EaeI y'GGCC_r
Cuts at: 227 1076 1250 1641 1668 3013 227
Size: 849 174 391 27 1345 705
Fragments arranged by size:
1345 849 705 391 174 27
EagI C'GGCC_G
Cuts at: 1076 1076
Size: 3491
EarI CTCTTCn'nnn_
Cuts at: 1514 1724 1514
Size: 210 3281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1699 2009 2155 3301 1699
Size: 310 146 1146 1889
Fragments arranged by size:
1889 1146 310 146
EcoICRI GAG'CTC
Cuts at: 503 503
Size: 3491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1748 2495 2763 1316
Size: 432 747 268 2044
Fragments arranged by size:
2044 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 198 327 630 198
Size: 129 303 3059
Fragments arranged by size:
3059 303 129
EcoRI G'AATT_C
Cuts at: 495 495
Size: 3491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 453 1510 438
Size: 15 1057 2419
Fragments arranged by size:
2419 1057 15
FspI TGC'GCA
Cuts at: 1272 1272
Size: 3491
HaeII r_GCGC'y
Cuts at: 190 293 925 1173 2195 3174 190
Size: 103 632 248 1022 979 507
Fragments arranged by size:
1022 979 632 507 248 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 1333 1363 1365 1395 331
Size: 32 970 30 2 30 2427
Fragments arranged by size:
2427 970 32 30 30 2
HincII GTy'rAC
Cuts at: 530 530
Size: 3491
KasI G'GCGC_C
Cuts at: 186 289 921 1169 186
Size: 103 632 248 2508
Fragments arranged by size:
2508 632 248 103
KpnI G_GTAC'C
Cuts at: 511 511
Size: 3491
MluI A'CGCG_T
Cuts at: 2924 2924
Size: 3491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2162 2346 2162
Size: 184 3307
MscI TGG'CCA
Cuts at: 1252 1252
Size: 3491
MslI CAynn'nnrTG
Cuts at: 1480 1607 1480
Size: 127 3364
MspA1I CmG'CkG
Cuts at: 123 602 916 1276 2289 2534 123
Size: 479 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 479 360 314 245
NaeI GCC'GGC
Cuts at: 215 1672 215
Size: 1457 2034
NarI GG'CG_CC
Cuts at: 187 290 922 1170 187
Size: 103 632 248 2508
Fragments arranged by size:
2508 632 248 103
NgoMIV G'CCGG_C
Cuts at: 213 1670 213
Size: 1457 2034
NruI TCG'CGA
Cuts at: 315 315
Size: 3491
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3491
NspI r_CATG'y
Cuts at: 544 1044 1951 544
Size: 500 907 2084
Fragments arranged by size:
2084 907 500
PciI A'CATG_T
Cuts at: 1947 1947
Size: 3491
PflMI CCAn_nnn'nTGG
Cuts at: 126 126
Size: 3491
PfoI T'CCnGG_A
Cuts at: 323 323
Size: 3491
PmeI GTTT'AAAC
Cuts at: 490 490
Size: 3491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 3459
PpuMI rG'GwC_Cy
Cuts at: 198 327 198
Size: 129 3362
PstI C_TGCA'G
Cuts at: 538 1223 538
Size: 685 2806
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3491
PvuII CAG'CTG
Cuts at: 123 916 1276 123
Size: 793 360 2338
Fragments arranged by size:
2338 793 360
RsrII CG'GwC_CG
Cuts at: 1686 1686
Size: 3491
SacI G_AGCT'C
Cuts at: 505 505
Size: 3491
SalI G'TCGA_C
Cuts at: 528 528
Size: 3491
SapI GCTCTTCn'nnn_
Cuts at: 1514 1724 1514
Size: 210 3281
SbfI CC_TGCA'GG
Cuts at: 538 538
Size: 3491
ScaI AGT'ACT
Cuts at: 3008 3008
Size: 3491
SfcI C'TryA_G
Cuts at: 33 534 1219 2212 2403 33
Size: 501 685 993 191 1121
Fragments arranged by size:
1121 993 685 501 191
SfoI GGC'GCC
Cuts at: 188 291 923 1171 188
Size: 103 632 248 2508
Fragments arranged by size:
2508 632 248 103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
SmaI CCC'GGG
Cuts at: 183 279 513 183
Size: 96 234 3161
Fragments arranged by size:
3161 234 96
SmlI C'TyrA_G
Cuts at: 298 445 646 1561 2053 2315 298
Size: 147 201 915 492 262 1474
Fragments arranged by size:
1474 915 492 262 201 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3491
SphI G_CATG'C
Cuts at: 544 1044 544
Size: 500 2991
StyI C'CwwG_G
Cuts at: 428 625 979 428
Size: 197 354 2940
Fragments arranged by size:
2940 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 1074 1207 1666 1875 470
Size: 604 133 459 209 2086
Fragments arranged by size:
2086 604 459 209 133
TatI w'GTAC_w
Cuts at: 3006 3006
Size: 3491
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3459
Tth111I GACn'n_nGTC
Cuts at: 1288 1288
Size: 3491
XbaI T'CTAG_A
Cuts at: 522 522
Size: 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 986 474
Size: 512 2979
XmaI C'CCGG_G
Cuts at: 181 277 511 181
Size: 96 234 3161
Fragments arranged by size:
3161 234 96
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI