Restriction map for
pF1KB8867
(Circular) (Six-base) MAPSORT of: pF1KB8867.seq Check: 8504 from: 1 to: 3479
pF1KB8867 3479 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 162 291 162
Size: 129 3350
AccI GT'mk_AC
Cuts at: 517 517
Size: 3479
Acc65I G'GTAC_C
Cuts at: 495 495
Size: 3479
AclI AA'CG_TT
Cuts at: 3319 3319
Size: 3479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1736 2483 2751 1304
Size: 432 747 268 2032
Fragments arranged by size:
2032 747 432 268
AflIII A'CryG_T
Cuts at: 831 1935 2912 831
Size: 1104 977 1398
Fragments arranged by size:
1398 1104 977
AgeI A'CCGG_T
Cuts at: 1867 1867
Size: 3479
AhdI GACnn_n'nnGTC
Cuts at: 336 336
Size: 3479
AlwNI CAG_nnn'CTG
Cuts at: 383 784 2351 383
Size: 401 1567 1511
Fragments arranged by size:
1567 1511 401
ApaLI G'TGCA_C
Cuts at: 2249 2249
Size: 3479
ApoI r'AATT_y
Cuts at: 483 2832 3088 483
Size: 2349 256 874
Fragments arranged by size:
2349 874 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3479
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
AvaI C'yCGr_G
Cuts at: 499 499
Size: 3479
BamHI G'GATC_C
Cuts at: 504 504
Size: 3479
BanI G'GyrC_C
Cuts at: 495 909 1157 1192 495
Size: 414 248 35 2782
Fragments arranged by size:
2782 414 248 35
BanII G_rGCy'C
Cuts at: 196 493 1523 196
Size: 297 1030 2152
Fragments arranged by size:
2152 1030 297
BbeI G_GCGC'C
Cuts at: 913 1161 913
Size: 248 3231
BbsI GAAGACnn'nnnn_
Cuts at: 335 335
Size: 3479
BbvCI CC'TCA_GC
Cuts at: 376 376
Size: 3479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3445
BciVI GTATCCnnnnn_n'
Cuts at: 1377 2138 3479 1377
Size: 761 1341 1377
Fragments arranged by size:
1377 1341 761
BclI T'GATC_A
Cuts at: 999 999
Size: 3479
BglI GCCn_nnn'nGGC
Cuts at: 429 429
Size: 3479
BglII A'GATC_T
Cuts at: 994 3031 994
Size: 2037 1442
BlpI GC'TnA_GC
Cuts at: 591 591
Size: 3479
Bme1580I G_kGCm'C
Cuts at: 1104 1197 2253 1104
Size: 93 1056 2330
Fragments arranged by size:
2330 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 691 772 1107 3251 691
Size: 81 335 2144 919
Fragments arranged by size:
2144 919 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3479
Bpu10I CC'TnA_GC
Cuts at: 376 376
Size: 3479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1534 2026 2324 655
Size: 879 492 298 1810
Fragments arranged by size:
1810 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3183 3183
Size: 3479
BsaAI yAC'GTr
Cuts at: 832 832
Size: 3479
BsaBI GATnn'nnATC
Cuts at: 6 993 3030 6
Size: 987 2037 455
Fragments arranged by size:
2037 987 455
BsaHI Gr'CG_yC
Cuts at: 910 1158 1860 3356 910
Size: 248 702 1496 1033
Fragments arranged by size:
1496 1033 702 248
BsaWI w'CCGG_w
Cuts at: 668 1189 1867 2141 2288 2699 668
Size: 521 678 274 147 411 1448
Fragments arranged by size:
1448 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 270
Size: 3479
BseYI C'CCAG_C
Cuts at: 125 317 904 1662 2239 2735 125
Size: 192 587 758 577 496 869
Fragments arranged by size:
869 758 587 577 496 192
BsiEI CG_ry'CG
Cuts at: 104 1021 1067 2275 104
Size: 917 46 1208 1308
Fragments arranged by size:
1308 1208 917 46
BsiHKAI G_wGCw'C
Cuts at: 269 493 1271 1461 2253 269
Size: 224 778 190 792 1495
Fragments arranged by size:
1495 792 778 224 190
Bsp1286I G_dGCh'C
Cuts at: 196 269 493 1104 1197 1271 1461 1523 2253
Size: 73 224 611 93 74 190 62 730
Cuts at: 2253 196
Size: 1422
Fragments arranged by size:
1422 730 611 224 190 93 74 73
62
BspEI T'CCGG_A
Cuts at: 2699 2699
Size: 3479
BspHI T'CATG_A
Cuts at: 2655 2655
Size: 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 291 529 743 1045 1426 162
Size: 129 238 214 302 381 2215
Fragments arranged by size:
2215 381 302 238 214 129
BsrBI CCG'CTC
Cuts at: 50 1771 1825 3309 3474 50
Size: 1721 54 1484 165 55
Fragments arranged by size:
1721 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1391 1391
Size: 3479
BsrFI r'CCGG_y
Cuts at: 1477 1658 1867 1477
Size: 181 209 3089
Fragments arranged by size:
3089 209 181
BssSI C'ACGA_G
Cuts at: 1750 2108 1750
Size: 358 3121
BstAPI GCAn_nnn'nTGC
Cuts at: 856 856
Size: 3479
BstBI TT'CG_AA
Cuts at: 260 1840 260
Size: 1580 1899
BstXI CCAn_nnnn'nTGG
Cuts at: 405 405
Size: 3479
BstYI r'GATC_y
Cuts at: 1 504 971 994 1329 1575 2587 2673 2685
Size: 503 467 23 335 246 1012 86 12
Cuts at: 2685 3031 1
Size: 346 449
Fragments arranged by size:
1012 503 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1429 1610 1773 1429
Size: 181 163 3135
Fragments arranged by size:
3135 181 163
BtsI GCAGTG_nn'
Cuts at: 146 722 146
Size: 576 2903
DraI TTT'AAA
Cuts at: 478 478
Size: 3479
DraIII CAC_nnn'GTG
Cuts at: 273 273
Size: 3479
DrdI GACnn_nn'nnGTC
Cuts at: 1185 2043 1185
Size: 858 2621
EaeI y'GGCC_r
Cuts at: 395 1064 1238 1629 1656 3001 395
Size: 669 174 391 27 1345 873
Fragments arranged by size:
1345 873 669 391 174 27
EagI C'GGCC_G
Cuts at: 1064 1064
Size: 3479
EarI CTCTTCn'nnn_
Cuts at: 257 1502 1712 257
Size: 1245 210 2024
Fragments arranged by size:
2024 1245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 447 1687 1997 2143 3289 447
Size: 1240 310 146 1146 637
Fragments arranged by size:
1240 1146 637 310 146
EcoICRI GAG'CTC
Cuts at: 491 491
Size: 3479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1736 2483 2751 1304
Size: 432 747 268 2032
Fragments arranged by size:
2032 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 199 618 199
Size: 419 3060
EcoRI G'AATT_C
Cuts at: 483 483
Size: 3479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1498 1498
Size: 3479
FspI TGC'GCA
Cuts at: 1260 1260
Size: 3479
HaeII r_GCGC'y
Cuts at: 913 1161 2183 3162 913
Size: 248 1022 979 1230
Fragments arranged by size:
1230 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 1321 1351 1353 1383 322
Size: 32 967 30 2 30 2418
Fragments arranged by size:
2418 967 32 30 30 2
HincII GTy'rAC
Cuts at: 518 518
Size: 3479
KasI G'GCGC_C
Cuts at: 909 1157 909
Size: 248 3231
KpnI G_GTAC'C
Cuts at: 499 499
Size: 3479
MluI A'CGCG_T
Cuts at: 2912 2912
Size: 3479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 474 2150 2334 474
Size: 1676 184 1619
Fragments arranged by size:
1676 1619 184
MscI TGG'CCA
Cuts at: 397 1240 397
Size: 843 2636
MslI CAynn'nnrTG
Cuts at: 1468 1595 1468
Size: 127 3352
MspA1I CmG'CkG
Cuts at: 352 590 904 1264 2277 2522 352
Size: 238 314 360 1013 245 1309
Fragments arranged by size:
1309 1013 360 314 245 238
NaeI GCC'GGC
Cuts at: 1660 1660
Size: 3479
NarI GG'CG_CC
Cuts at: 910 1158 910
Size: 248 3231
NgoMIV G'CCGG_C
Cuts at: 1658 1658
Size: 3479
NspI r_CATG'y
Cuts at: 532 1032 1939 532
Size: 500 907 2072
Fragments arranged by size:
2072 907 500
PciI A'CATG_T
Cuts at: 1935 1935
Size: 3479
PmeI GTTT'AAAC
Cuts at: 478 478
Size: 3479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 3447
PstI C_TGCA'G
Cuts at: 136 173 240 526 1211 136
Size: 37 67 286 685 2404
Fragments arranged by size:
2404 685 286 67 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3479
PvuII CAG'CTG
Cuts at: 352 904 1264 352
Size: 552 360 2567
Fragments arranged by size:
2567 552 360
RsrII CG'GwC_CG
Cuts at: 1674 1674
Size: 3479
SacI G_AGCT'C
Cuts at: 493 493
Size: 3479
SalI G'TCGA_C
Cuts at: 516 516
Size: 3479
SapI GCTCTTCn'nnn_
Cuts at: 257 1502 1712 257
Size: 1245 210 2024
Fragments arranged by size:
2024 1245 210
SbfI CC_TGCA'GG
Cuts at: 526 526
Size: 3479
ScaI AGT'ACT
Cuts at: 2996 2996
Size: 3479
SfcI C'TryA_G
Cuts at: 33 132 169 236 522 1207 2200 2391 33
Size: 99 37 67 286 685 993 191 1121
Fragments arranged by size:
1121 993 685 286 191 99 67 37
SfoI GGC'GCC
Cuts at: 911 1159 911
Size: 248 3231
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
SmaI CCC'GGG
Cuts at: 501 501
Size: 3479
SmlI C'TyrA_G
Cuts at: 634 1549 2041 2303 634
Size: 915 492 262 1810
Fragments arranged by size:
1810 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3479
SphI G_CATG'C
Cuts at: 532 1032 532
Size: 500 2979
StyI C'CwwG_G
Cuts at: 613 967 613
Size: 354 3125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1062 1195 1654 1863 277
Size: 785 133 459 209 1893
Fragments arranged by size:
1893 785 459 209 133
TatI w'GTAC_w
Cuts at: 2994 2994
Size: 3479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3447
Tth111I GACn'n_nGTC
Cuts at: 1276 1276
Size: 3479
XbaI T'CTAG_A
Cuts at: 510 510
Size: 3479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 974 114
Size: 860 2619
XmaI C'CCGG_G
Cuts at: 499 499
Size: 3479
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI