Restriction map for
pF1KB8864
(Circular) (Six-base) MAPSORT of: pF1KB8864.seq Check: 3202 from: 1 to: 3443
pF1KB8864 3443 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 282 291 282
Size: 9 3434
AccI GT'mk_AC
Cuts at: 481 481
Size: 3443
Acc65I G'GTAC_C
Cuts at: 459 459
Size: 3443
AclI AA'CG_TT
Cuts at: 437 3283 437
Size: 2846 597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1700 2447 2715 1268
Size: 432 747 268 1996
Fragments arranged by size:
1996 747 432 268
AflIII A'CryG_T
Cuts at: 795 1899 2876 795
Size: 1104 977 1362
Fragments arranged by size:
1362 1104 977
AgeI A'CCGG_T
Cuts at: 1831 1831
Size: 3443
AlwNI CAG_nnn'CTG
Cuts at: 404 748 2315 404
Size: 344 1567 1532
Fragments arranged by size:
1567 1532 344
ApaI G_GGCC'C
Cuts at: 322 322
Size: 3443
ApaLI G'TGCA_C
Cuts at: 2213 2213
Size: 3443
ApoI r'AATT_y
Cuts at: 447 2796 3052 447
Size: 2349 256 838
Fragments arranged by size:
2349 838 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3443
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
AvaI C'yCGr_G
Cuts at: 463 463
Size: 3443
BamHI G'GATC_C
Cuts at: 468 468
Size: 3443
BanI G'GyrC_C
Cuts at: 459 873 1121 1156 459
Size: 414 248 35 2746
Fragments arranged by size:
2746 414 248 35
BanII G_rGCy'C
Cuts at: 322 457 1487 322
Size: 135 1030 2278
Fragments arranged by size:
2278 1030 135
BbeI G_GCGC'C
Cuts at: 877 1125 877
Size: 248 3195
BciVI GTATCCnnnnn_n'
Cuts at: 1341 2102 3443 1341
Size: 761 1341 1341
Fragments arranged by size:
1341 1341 761
BclI T'GATC_A
Cuts at: 963 963
Size: 3443
BglII A'GATC_T
Cuts at: 958 2995 958
Size: 2037 1406
BlpI GC'TnA_GC
Cuts at: 555 555
Size: 3443
Bme1580I G_kGCm'C
Cuts at: 322 1068 1161 2217 322
Size: 746 93 1056 1548
Fragments arranged by size:
1548 1056 746 93
BmrI ACTGGGnnnn_n'
Cuts at: 655 736 1071 3215 655
Size: 81 335 2144 883
Fragments arranged by size:
2144 883 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 442 373
Size: 69 3374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1498 1990 2288 619
Size: 879 492 298 1774
Fragments arranged by size:
1774 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3147 3147
Size: 3443
BsaAI yAC'GTr
Cuts at: 796 796
Size: 3443
BsaBI GATnn'nnATC
Cuts at: 6 957 2994 6
Size: 951 2037 455
Fragments arranged by size:
2037 951 455
BsaHI Gr'CG_yC
Cuts at: 874 1122 1824 3320 874
Size: 248 702 1496 997
Fragments arranged by size:
1496 997 702 248
BsaWI w'CCGG_w
Cuts at: 632 1153 1831 2105 2252 2663 632
Size: 521 678 274 147 411 1412
Fragments arranged by size:
1412 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 168 309 868 1626 2203 2699 168
Size: 141 559 758 577 496 912
Fragments arranged by size:
912 758 577 559 496 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 347
Size: 3443
BsiEI CG_ry'CG
Cuts at: 104 985 1031 2239 104
Size: 881 46 1208 1308
Fragments arranged by size:
1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 457 1235 1425 2217 457
Size: 778 190 792 1683
Fragments arranged by size:
1683 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 322 457 1068 1161 1235 1425 1487 2217 322
Size: 135 611 93 74 190 62 730 1548
Fragments arranged by size:
1548 730 611 190 135 93 74 62
BspEI T'CCGG_A
Cuts at: 2663 2663
Size: 3443
BspHI T'CATG_A
Cuts at: 2619 2619
Size: 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 282 291 296 493 707 1009 1390 282
Size: 9 5 197 214 302 381 2335
Fragments arranged by size:
2335 381 302 214 197 9 5
BsrBI CCG'CTC
Cuts at: 50 1735 1789 3273 3438 50
Size: 1685 54 1484 165 55
Fragments arranged by size:
1685 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1355 1355
Size: 3443
BsrFI r'CCGG_y
Cuts at: 1441 1622 1831 1441
Size: 181 209 3053
Fragments arranged by size:
3053 209 181
BssSI C'ACGA_G
Cuts at: 1714 2072 1714
Size: 358 3085
BstAPI GCAn_nnn'nTGC
Cuts at: 820 820
Size: 3443
BstBI TT'CG_AA
Cuts at: 1804 1804
Size: 3443
BstYI r'GATC_y
Cuts at: 1 468 935 958 1293 1539 2551 2637 2649
Size: 467 467 23 335 246 1012 86 12
Cuts at: 2649 2995 1
Size: 346 449
Fragments arranged by size:
1012 467 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1393 1574 1737 1393
Size: 181 163 3099
Fragments arranged by size:
3099 181 163
BtsI GCAGTG_nn'
Cuts at: 686 686
Size: 3443
DraI TTT'AAA
Cuts at: 442 442
Size: 3443
DraIII CAC_nnn'GTG
Cuts at: 213 213
Size: 3443
DrdI GACnn_nn'nnGTC
Cuts at: 1149 2007 1149
Size: 858 2585
EaeI y'GGCC_r
Cuts at: 1028 1202 1593 1620 2965 1028
Size: 174 391 27 1345 1506
Fragments arranged by size:
1506 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1028 1028
Size: 3443
EarI CTCTTCn'nnn_
Cuts at: 1466 1676 1466
Size: 210 3233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1651 1961 2107 3253 1651
Size: 310 146 1146 1841
Fragments arranged by size:
1841 1146 310 146
EcoICRI GAG'CTC
Cuts at: 455 455
Size: 3443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 442 1268 1700 2447 2715 373
Size: 69 826 432 747 268 1101
Fragments arranged by size:
1101 826 747 432 268 69
EcoNI CCTnn'n_nnAGG
Cuts at: 326 326
Size: 3443
EcoO109I rG'GnC_Cy
Cuts at: 160 285 318 319 359 582 160
Size: 125 33 1 40 223 3021
Fragments arranged by size:
3021 223 125 40 33 1
EcoRI G'AATT_C
Cuts at: 447 447
Size: 3443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1462 1462
Size: 3443
FspI TGC'GCA
Cuts at: 1224 1224
Size: 3443
HaeII r_GCGC'y
Cuts at: 145 877 1125 2147 3126 145
Size: 732 248 1022 979 462
Fragments arranged by size:
1022 979 732 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1315 1317 1347 1285
Size: 30 2 30 3381
Fragments arranged by size:
3381 30 30 2
HincII GTy'rAC
Cuts at: 482 482
Size: 3443
KasI G'GCGC_C
Cuts at: 873 1121 873
Size: 248 3195
KpnI G_GTAC'C
Cuts at: 463 463
Size: 3443
MluI A'CGCG_T
Cuts at: 2876 2876
Size: 3443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2298 2114
Size: 184 3259
MscI TGG'CCA
Cuts at: 1204 1204
Size: 3443
MslI CAynn'nnrTG
Cuts at: 1432 1559 1432
Size: 127 3316
MspA1I CmG'CkG
Cuts at: 420 554 868 1228 2241 2486 420
Size: 134 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 360 314 245 134
NaeI GCC'GGC
Cuts at: 1624 1624
Size: 3443
NarI GG'CG_CC
Cuts at: 874 1122 874
Size: 248 3195
NcoI C'CATG_G
Cuts at: 107 107
Size: 3443
NgoMIV G'CCGG_C
Cuts at: 1622 1622
Size: 3443
NspI r_CATG'y
Cuts at: 496 996 1903 496
Size: 500 907 2036
Fragments arranged by size:
2036 907 500
PciI A'CATG_T
Cuts at: 1899 1899
Size: 3443
PmeI GTTT'AAAC
Cuts at: 442 442
Size: 3443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 2582
Size: 32 3411
PpuMI rG'GwC_Cy
Cuts at: 160 285 359 160
Size: 125 74 3244
Fragments arranged by size:
3244 125 74
PspOMI G'GGCC_C
Cuts at: 318 318
Size: 3443
PstI C_TGCA'G
Cuts at: 293 341 368 490 1175 293
Size: 48 27 122 685 2561
Fragments arranged by size:
2561 685 122 48 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3443
PvuII CAG'CTG
Cuts at: 420 868 1228 420
Size: 448 360 2635
Fragments arranged by size:
2635 448 360
RsrII CG'GwC_CG
Cuts at: 1638 1638
Size: 3443
SacI G_AGCT'C
Cuts at: 457 457
Size: 3443
SalI G'TCGA_C
Cuts at: 480 480
Size: 3443
SanDI GG'GwC_CC
Cuts at: 359 359
Size: 3443
SapI GCTCTTCn'nnn_
Cuts at: 1466 1676 1466
Size: 210 3233
SbfI CC_TGCA'GG
Cuts at: 293 490 293
Size: 197 3246
ScaI AGT'ACT
Cuts at: 2960 2960
Size: 3443
SexAI A'CCwGG_T
Cuts at: 209 209
Size: 3443
SfcI C'TryA_G
Cuts at: 33 289 337 364 486 1171 2164 2355 33
Size: 256 48 27 122 685 993 191 1121
Fragments arranged by size:
1121 993 685 256 191 122 48 27
SfoI GGC'GCC
Cuts at: 875 1123 875
Size: 248 3195
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
SmaI CCC'GGG
Cuts at: 465 465
Size: 3443
SmlI C'TyrA_G
Cuts at: 598 1513 2005 2267 598
Size: 915 492 262 1774
Fragments arranged by size:
1774 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3443
SphI G_CATG'C
Cuts at: 496 996 496
Size: 500 2943
StyI C'CwwG_G
Cuts at: 107 344 577 931 107
Size: 237 233 354 2619
Fragments arranged by size:
2619 354 237 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 1026 1159 1618 1827 276
Size: 750 133 459 209 1892
Fragments arranged by size:
1892 750 459 209 133
TatI w'GTAC_w
Cuts at: 2958 2958
Size: 3443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3411
Tth111I GACn'n_nGTC
Cuts at: 1240 1240
Size: 3443
XbaI T'CTAG_A
Cuts at: 474 474
Size: 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 938
Size: 3443
XmaI C'CCGG_G
Cuts at: 463 463
Size: 3443
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI