Restriction map for
pF1KB8860
(Circular) (Six-base) MAPSORT of: pF1KB8860.seq Check: 2025 from: 1 to: 3404
pF1KB8860 3404 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 442 442
Size: 3404
Acc65I G'GTAC_C
Cuts at: 420 420
Size: 3404
AclI AA'CG_TT
Cuts at: 3244 3244
Size: 3404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1661 2408 2676 1229
Size: 432 747 268 1957
Fragments arranged by size:
1957 747 432 268
AflIII A'CryG_T
Cuts at: 756 1860 2837 756
Size: 1104 977 1323
Fragments arranged by size:
1323 1104 977
AgeI A'CCGG_T
Cuts at: 1792 1792
Size: 3404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 205
Size: 32 3372
AlwNI CAG_nnn'CTG
Cuts at: 709 2276 709
Size: 1567 1837
ApaLI G'TGCA_C
Cuts at: 2174 2174
Size: 3404
ApoI r'AATT_y
Cuts at: 160 408 2757 3013 160
Size: 248 2349 256 551
Fragments arranged by size:
2349 551 256 248
AseI AT'TA_AT
Cuts at: 20 20
Size: 3404
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3404
AvaI C'yCGr_G
Cuts at: 424 424
Size: 3404
BamHI G'GATC_C
Cuts at: 429 429
Size: 3404
BanI G'GyrC_C
Cuts at: 420 834 1082 1117 420
Size: 414 248 35 2707
Fragments arranged by size:
2707 414 248 35
BanII G_rGCy'C
Cuts at: 418 1448 418
Size: 1030 2374
BbeI G_GCGC'C
Cuts at: 838 1086 838
Size: 248 3156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3370
BciVI GTATCCnnnnn_n'
Cuts at: 1302 2063 3404 1302
Size: 761 1341 1302
Fragments arranged by size:
1341 1302 761
BclI T'GATC_A
Cuts at: 924 924
Size: 3404
BglII A'GATC_T
Cuts at: 919 2956 919
Size: 2037 1367
BlpI GC'TnA_GC
Cuts at: 516 516
Size: 3404
Bme1580I G_kGCm'C
Cuts at: 1029 1122 2178 1029
Size: 93 1056 2255
Fragments arranged by size:
2255 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 616 697 1032 3176 616
Size: 81 335 2144 844
Fragments arranged by size:
2144 844 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1459 1951 2249 580
Size: 879 492 298 1735
Fragments arranged by size:
1735 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3108 3108
Size: 3404
BsaAI yAC'GTr
Cuts at: 757 757
Size: 3404
BsaBI GATnn'nnATC
Cuts at: 6 918 2955 6
Size: 912 2037 455
Fragments arranged by size:
2037 912 455
BsaHI Gr'CG_yC
Cuts at: 835 1083 1785 3281 835
Size: 248 702 1496 958
Fragments arranged by size:
1496 958 702 248
BsaWI w'CCGG_w
Cuts at: 593 1114 1792 2066 2213 2624 593
Size: 521 678 274 147 411 1373
Fragments arranged by size:
1373 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 166 829 1587 2164 2660 166
Size: 663 758 577 496 910
Fragments arranged by size:
910 758 663 577 496
BsiEI CG_ry'CG
Cuts at: 104 946 992 2200 104
Size: 842 46 1208 1308
Fragments arranged by size:
1308 1208 842 46
BsiHKAI G_wGCw'C
Cuts at: 418 1196 1386 2178 418
Size: 778 190 792 1644
Fragments arranged by size:
1644 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 418 1029 1122 1196 1386 1448 2178 418
Size: 611 93 74 190 62 730 1644
Fragments arranged by size:
1644 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2624 2624
Size: 3404
BspHI T'CATG_A
Cuts at: 2580 2580
Size: 3404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 454 668 970 1351 454
Size: 214 302 381 2507
Fragments arranged by size:
2507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1696 1750 3234 3399 50
Size: 1646 54 1484 165 55
Fragments arranged by size:
1646 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 347 363 1316 347
Size: 16 953 2435
Fragments arranged by size:
2435 953 16
BsrFI r'CCGG_y
Cuts at: 1402 1583 1792 1402
Size: 181 209 3014
Fragments arranged by size:
3014 209 181
BssSI C'ACGA_G
Cuts at: 1675 2033 1675
Size: 358 3046
BstAPI GCAn_nnn'nTGC
Cuts at: 781 781
Size: 3404
BstBI TT'CG_AA
Cuts at: 1765 1765
Size: 3404
BstYI r'GATC_y
Cuts at: 1 429 896 919 1254 1500 2512 2598 2610
Size: 428 467 23 335 246 1012 86 12
Cuts at: 2610 2956 1
Size: 346 449
Fragments arranged by size:
1012 467 449 428 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 131 131
Size: 3404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1354 1535 1698 1354
Size: 181 163 3060
Fragments arranged by size:
3060 181 163
BtsI GCAGTG_nn'
Cuts at: 647 647
Size: 3404
DraI TTT'AAA
Cuts at: 403 403
Size: 3404
DrdI GACnn_nn'nnGTC
Cuts at: 1110 1968 1110
Size: 858 2546
EaeI y'GGCC_r
Cuts at: 989 1163 1554 1581 2926 989
Size: 174 391 27 1345 1467
Fragments arranged by size:
1467 1345 391 174 27
EagI C'GGCC_G
Cuts at: 989 989
Size: 3404
EarI CTCTTCn'nnn_
Cuts at: 1427 1637 1427
Size: 210 3194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1612 1922 2068 3214 1612
Size: 310 146 1146 1802
Fragments arranged by size:
1802 1146 310 146
EcoICRI GAG'CTC
Cuts at: 416 416
Size: 3404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1661 2408 2676 1229
Size: 432 747 268 1957
Fragments arranged by size:
1957 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 195 279 543 195
Size: 84 264 3056
Fragments arranged by size:
3056 264 84
EcoRI G'AATT_C
Cuts at: 408 408
Size: 3404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1423 1423
Size: 3404
FspI TGC'GCA
Cuts at: 1185 1185
Size: 3404
HaeII r_GCGC'y
Cuts at: 838 1086 2108 3087 838
Size: 248 1022 979 1155
Fragments arranged by size:
1155 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1246 1276 1278 1308 1246
Size: 30 2 30 3342
Fragments arranged by size:
3342 30 30 2
HincII GTy'rAC
Cuts at: 443 443
Size: 3404
KasI G'GCGC_C
Cuts at: 834 1082 834
Size: 248 3156
KpnI G_GTAC'C
Cuts at: 424 424
Size: 3404
MluI A'CGCG_T
Cuts at: 2837 2837
Size: 3404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2259 2075
Size: 184 3220
MscI TGG'CCA
Cuts at: 1165 1165
Size: 3404
MslI CAynn'nnrTG
Cuts at: 1393 1520 1393
Size: 127 3277
MspA1I CmG'CkG
Cuts at: 515 829 1189 2202 2447 515
Size: 314 360 1013 245 1472
Fragments arranged by size:
1472 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1585 1585
Size: 3404
NarI GG'CG_CC
Cuts at: 835 1083 835
Size: 248 3156
NcoI C'CATG_G
Cuts at: 131 131
Size: 3404
NgoMIV G'CCGG_C
Cuts at: 1583 1583
Size: 3404
NspI r_CATG'y
Cuts at: 356 457 957 1864 356
Size: 101 500 907 1896
Fragments arranged by size:
1896 907 500 101
PciI A'CATG_T
Cuts at: 1860 1860
Size: 3404
PmeI GTTT'AAAC
Cuts at: 403 403
Size: 3404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 2543
Size: 32 3372
PpuMI rG'GwC_Cy
Cuts at: 195 279 195
Size: 84 3320
PstI C_TGCA'G
Cuts at: 451 1136 451
Size: 685 2719
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3404
PvuII CAG'CTG
Cuts at: 829 1189 829
Size: 360 3044
RsrII CG'GwC_CG
Cuts at: 1599 1599
Size: 3404
SacI G_AGCT'C
Cuts at: 418 418
Size: 3404
SalI G'TCGA_C
Cuts at: 441 441
Size: 3404
SapI GCTCTTCn'nnn_
Cuts at: 1427 1637 1427
Size: 210 3194
SbfI CC_TGCA'GG
Cuts at: 451 451
Size: 3404
ScaI AGT'ACT
Cuts at: 206 2921 206
Size: 2715 689
SfcI C'TryA_G
Cuts at: 33 447 1132 2125 2316 33
Size: 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 191
SfoI GGC'GCC
Cuts at: 836 1084 836
Size: 248 3156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3404
SmaI CCC'GGG
Cuts at: 426 426
Size: 3404
SmlI C'TyrA_G
Cuts at: 559 1474 1966 2228 559
Size: 915 492 262 1735
Fragments arranged by size:
1735 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3404
SphI G_CATG'C
Cuts at: 356 457 957 356
Size: 101 500 2803
Fragments arranged by size:
2803 500 101
StyI C'CwwG_G
Cuts at: 131 538 892 131
Size: 407 354 2643
Fragments arranged by size:
2643 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 987 1120 1579 1788 987
Size: 133 459 209 2603
Fragments arranged by size:
2603 459 209 133
TatI w'GTAC_w
Cuts at: 204 372 2919 204
Size: 168 2547 689
Fragments arranged by size:
2547 689 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3372
Tth111I GACn'n_nGTC
Cuts at: 1201 1201
Size: 3404
XbaI T'CTAG_A
Cuts at: 435 435
Size: 3404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 899 295
Size: 604 2800
XmaI C'CCGG_G
Cuts at: 424 424
Size: 3404
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI