Restriction map for
pF1KB8850
(Circular) (Six-base) MAPSORT of: pF1KB8850.seq Check: 2433 from: 1 to: 3326
pF1KB8850 3326 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 364 364
Size: 3326
Acc65I G'GTAC_C
Cuts at: 342 342
Size: 3326
AclI AA'CG_TT
Cuts at: 3166 3166
Size: 3326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1151 1583 2330 2598 260
Size: 891 432 747 268 988
Fragments arranged by size:
988 891 747 432 268
AflII C'TTAA_G
Cuts at: 163 163
Size: 3326
AflIII A'CryG_T
Cuts at: 678 1782 2759 678
Size: 1104 977 1245
Fragments arranged by size:
1245 1104 977
AgeI A'CCGG_T
Cuts at: 1714 1714
Size: 3326
AlwNI CAG_nnn'CTG
Cuts at: 631 2198 631
Size: 1567 1759
ApaLI G'TGCA_C
Cuts at: 2096 2096
Size: 3326
ApoI r'AATT_y
Cuts at: 330 2679 2935 330
Size: 2349 256 721
Fragments arranged by size:
2349 721 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3326
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3326
AvaI C'yCGr_G
Cuts at: 346 346
Size: 3326
BamHI G'GATC_C
Cuts at: 351 351
Size: 3326
BanI G'GyrC_C
Cuts at: 342 756 1004 1039 342
Size: 414 248 35 2629
Fragments arranged by size:
2629 414 248 35
BanII G_rGCy'C
Cuts at: 340 1370 340
Size: 1030 2296
BbeI G_GCGC'C
Cuts at: 760 1008 760
Size: 248 3078
BbsI GAAGACnn'nnnn_
Cuts at: 254 254
Size: 3326
BciVI GTATCCnnnnn_n'
Cuts at: 1224 1985 3326 1224
Size: 761 1341 1224
Fragments arranged by size:
1341 1224 761
BclI T'GATC_A
Cuts at: 846 846
Size: 3326
BglII A'GATC_T
Cuts at: 841 2878 841
Size: 2037 1289
BlpI GC'TnA_GC
Cuts at: 438 438
Size: 3326
Bme1580I G_kGCm'C
Cuts at: 951 1044 2100 951
Size: 93 1056 2177
Fragments arranged by size:
2177 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 538 619 954 3098 538
Size: 81 335 2144 766
Fragments arranged by size:
2144 766 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1381 1873 2171 502
Size: 879 492 298 1657
Fragments arranged by size:
1657 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3030 3030
Size: 3326
BsaAI yAC'GTr
Cuts at: 679 679
Size: 3326
BsaBI GATnn'nnATC
Cuts at: 6 840 2877 6
Size: 834 2037 455
Fragments arranged by size:
2037 834 455
BsaHI Gr'CG_yC
Cuts at: 757 1005 1707 3203 757
Size: 248 702 1496 880
Fragments arranged by size:
1496 880 702 248
BsaWI w'CCGG_w
Cuts at: 515 1036 1714 1988 2135 2546 515
Size: 521 678 274 147 411 1295
Fragments arranged by size:
1295 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 286
Size: 30 3296
BseYI C'CCAG_C
Cuts at: 751 1509 2086 2582 751
Size: 758 577 496 1495
Fragments arranged by size:
1495 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 868 914 2122 104
Size: 764 46 1208 1308
Fragments arranged by size:
1308 1208 764 46
BsiHKAI G_wGCw'C
Cuts at: 340 1118 1308 2100 340
Size: 778 190 792 1566
Fragments arranged by size:
1566 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 340 951 1044 1118 1308 1370 2100 340
Size: 611 93 74 190 62 730 1566
Fragments arranged by size:
1566 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2546 2546
Size: 3326
BspHI T'CATG_A
Cuts at: 2502 2502
Size: 3326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 376 590 892 1273 376
Size: 214 302 381 2429
Fragments arranged by size:
2429 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1618 1672 3156 3321 50
Size: 1568 54 1484 165 55
Fragments arranged by size:
1568 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1238 1238
Size: 3326
BsrFI r'CCGG_y
Cuts at: 1324 1505 1714 1324
Size: 181 209 2936
Fragments arranged by size:
2936 209 181
BssSI C'ACGA_G
Cuts at: 1597 1955 1597
Size: 358 2968
BstAPI GCAn_nnn'nTGC
Cuts at: 703 703
Size: 3326
BstBI TT'CG_AA
Cuts at: 1687 1687
Size: 3326
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 3326
BstYI r'GATC_y
Cuts at: 1 351 818 841 1176 1422 2434 2520 2532
Size: 350 467 23 335 246 1012 86 12
Cuts at: 2532 2878 1
Size: 346 449
Fragments arranged by size:
1012 467 449 350 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1276 1457 1620 1276
Size: 181 163 2982
Fragments arranged by size:
2982 181 163
BtsI GCAGTG_nn'
Cuts at: 569 569
Size: 3326
DraI TTT'AAA
Cuts at: 325 325
Size: 3326
DrdI GACnn_nn'nnGTC
Cuts at: 1032 1890 1032
Size: 858 2468
EaeI y'GGCC_r
Cuts at: 110 911 1085 1476 1503 2848 110
Size: 801 174 391 27 1345 588
Fragments arranged by size:
1345 801 588 391 174 27
EagI C'GGCC_G
Cuts at: 911 911
Size: 3326
EarI CTCTTCn'nnn_
Cuts at: 1349 1559 1349
Size: 210 3116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1534 1844 1990 3136 1534
Size: 310 146 1146 1724
Fragments arranged by size:
1724 1146 310 146
EcoICRI GAG'CTC
Cuts at: 338 338
Size: 3326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1151 1583 2330 2598 260
Size: 891 432 747 268 988
Fragments arranged by size:
988 891 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 465 465
Size: 3326
EcoRI G'AATT_C
Cuts at: 330 330
Size: 3326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1345 1345
Size: 3326
FspI TGC'GCA
Cuts at: 1107 1107
Size: 3326
HaeII r_GCGC'y
Cuts at: 760 1008 2030 3009 760
Size: 248 1022 979 1077
Fragments arranged by size:
1077 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1168 1198 1200 1230 1168
Size: 30 2 30 3264
Fragments arranged by size:
3264 30 30 2
HincII GTy'rAC
Cuts at: 365 365
Size: 3326
KasI G'GCGC_C
Cuts at: 756 1004 756
Size: 248 3078
KpnI G_GTAC'C
Cuts at: 346 346
Size: 3326
MluI A'CGCG_T
Cuts at: 2759 2759
Size: 3326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1997 2181 1997
Size: 184 3142
MscI TGG'CCA
Cuts at: 112 1087 112
Size: 975 2351
MslI CAynn'nnrTG
Cuts at: 1315 1442 1315
Size: 127 3199
MspA1I CmG'CkG
Cuts at: 208 437 751 1111 2124 2369 208
Size: 229 314 360 1013 245 1165
Fragments arranged by size:
1165 1013 360 314 245 229
NaeI GCC'GGC
Cuts at: 1507 1507
Size: 3326
NarI GG'CG_CC
Cuts at: 757 1005 757
Size: 248 3078
NcoI C'CATG_G
Cuts at: 107 107
Size: 3326
NgoMIV G'CCGG_C
Cuts at: 1505 1505
Size: 3326
NspI r_CATG'y
Cuts at: 241 379 879 1786 241
Size: 138 500 907 1781
Fragments arranged by size:
1781 907 500 138
PciI A'CATG_T
Cuts at: 1782 1782
Size: 3326
PmeI GTTT'AAAC
Cuts at: 325 325
Size: 3326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 3294
PstI C_TGCA'G
Cuts at: 213 373 1058 213
Size: 160 685 2481
Fragments arranged by size:
2481 685 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3326
PvuII CAG'CTG
Cuts at: 208 751 1111 208
Size: 543 360 2423
Fragments arranged by size:
2423 543 360
RsrII CG'GwC_CG
Cuts at: 1521 1521
Size: 3326
SacI G_AGCT'C
Cuts at: 340 340
Size: 3326
SalI G'TCGA_C
Cuts at: 363 363
Size: 3326
SapI GCTCTTCn'nnn_
Cuts at: 1349 1559 1349
Size: 210 3116
SbfI CC_TGCA'GG
Cuts at: 373 373
Size: 3326
ScaI AGT'ACT
Cuts at: 2843 2843
Size: 3326
SfcI C'TryA_G
Cuts at: 33 209 369 1054 2047 2238 33
Size: 176 160 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 176 160
SfoI GGC'GCC
Cuts at: 758 1006 758
Size: 248 3078
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3326
SmaI CCC'GGG
Cuts at: 348 348
Size: 3326
SmlI C'TyrA_G
Cuts at: 163 481 1396 1888 2150 163
Size: 318 915 492 262 1339
Fragments arranged by size:
1339 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3326
SphI G_CATG'C
Cuts at: 379 879 379
Size: 500 2826
StyI C'CwwG_G
Cuts at: 107 200 242 460 814 107
Size: 93 42 218 354 2619
Fragments arranged by size:
2619 354 218 93 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 909 1042 1501 1710 909
Size: 133 459 209 2525
Fragments arranged by size:
2525 459 209 133
TatI w'GTAC_w
Cuts at: 2841 2841
Size: 3326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3294
Tth111I GACn'n_nGTC
Cuts at: 1123 1123
Size: 3326
XbaI T'CTAG_A
Cuts at: 357 357
Size: 3326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 821 821
Size: 3326
XmaI C'CCGG_G
Cuts at: 346 346
Size: 3326
XmnI GAAnn'nnTTC
Cuts at: 304 304
Size: 3326
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI