Restriction map for
pF1KB8846
(Circular) (Six-base) MAPSORT of: pF1KB8846.seq Check: 2538 from: 1 to: 4718
pF1KB8846 4718 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1584 1584
Size: 4718
AccI GT'mk_AC
Cuts at: 775 914 1639 1756 775
Size: 139 725 117 3737
Fragments arranged by size:
3737 725 139 117
Acc65I G'GTAC_C
Cuts at: 1734 1734
Size: 4718
AclI AA'CG_TT
Cuts at: 4558 4558
Size: 4718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1068 2543 2975 3722 3990 700
Size: 368 1475 432 747 268 1428
Fragments arranged by size:
1475 1428 747 432 368 268
AflIII A'CryG_T
Cuts at: 729 1276 2070 3174 4151 729
Size: 547 794 1104 977 1296
Fragments arranged by size:
1296 1104 977 794 547
AgeI A'CCGG_T
Cuts at: 3106 3106
Size: 4718
AhdI GACnn_n'nnGTC
Cuts at: 642 642
Size: 4718
AlwNI CAG_nnn'CTG
Cuts at: 1614 1665 2023 3590 1614
Size: 51 358 1567 2742
Fragments arranged by size:
2742 1567 358 51
ApaLI G'TGCA_C
Cuts at: 3488 3488
Size: 4718
ApoI r'AATT_y
Cuts at: 1140 1722 4071 4327 1140
Size: 582 2349 256 1531
Fragments arranged by size:
2349 1531 582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
AvaI C'yCGr_G
Cuts at: 597 895 1005 1738 597
Size: 298 110 733 3577
Fragments arranged by size:
3577 733 298 110
BamHI G'GATC_C
Cuts at: 1743 1743
Size: 4718
BanI G'GyrC_C
Cuts at: 979 1734 2148 2396 2431 979
Size: 755 414 248 35 3266
Fragments arranged by size:
3266 755 414 248 35
BanII G_rGCy'C
Cuts at: 400 462 688 1006 1732 2762 400
Size: 62 226 318 726 1030 2356
Fragments arranged by size:
2356 1030 726 318 226 62
BbeI G_GCGC'C
Cuts at: 2152 2400 2152
Size: 248 4470
BbvCI CC'TCA_GC
Cuts at: 429 429
Size: 4718
BciVI GTATCCnnnnn_n'
Cuts at: 812 2616 3377 4718 812
Size: 1804 761 1341 812
Fragments arranged by size:
1804 1341 812 761
BclI T'GATC_A
Cuts at: 2238 2238
Size: 4718
BglI GCCn_nnn'nGGC
Cuts at: 525 1114 525
Size: 589 4129
BglII A'GATC_T
Cuts at: 941 2233 4270 941
Size: 1292 2037 1389
Fragments arranged by size:
2037 1389 1292
BlpI GC'TnA_GC
Cuts at: 1830 1830
Size: 4718
Bme1580I G_kGCm'C
Cuts at: 385 2343 2436 3492 385
Size: 1958 93 1056 1611
Fragments arranged by size:
1958 1611 1056 93
BmgBI CAC'GTC
Cuts at: 453 482 453
Size: 29 4689
BmrI ACTGGGnnnn_n'
Cuts at: 1272 1570 1930 2011 2346 4490 1272
Size: 298 360 81 335 2144 1500
Fragments arranged by size:
2144 1500 360 335 298 81
BmtI G_CTAG'C
Cuts at: 205 1303 205
Size: 1098 3620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 4718
Bpu10I CC'TnA_GC
Cuts at: 429 429
Size: 4718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1386 1894 2773 3265 3563 857
Size: 529 508 879 492 298 2012
Fragments arranged by size:
2012 879 529 508 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 538 643 698 1535 4422 538
Size: 105 55 837 2887 834
Fragments arranged by size:
2887 837 834 105 55
BsaAI yAC'GTr
Cuts at: 656 1113 2071 656
Size: 457 958 3303
Fragments arranged by size:
3303 958 457
BsaBI GATnn'nnATC
Cuts at: 6 2232 4269 6
Size: 2226 2037 455
Fragments arranged by size:
2226 2037 455
BsaHI Gr'CG_yC
Cuts at: 617 2149 2397 3099 4595 617
Size: 1532 248 702 1496 740
Fragments arranged by size:
1532 1496 740 702 248
BsaWI w'CCGG_w
Cuts at: 620 1907 2428 3106 3380 3527 3938 620
Size: 1287 521 678 274 147 411 1400
Fragments arranged by size:
1400 1287 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 813 843 403
Size: 30 380 30 4278
Fragments arranged by size:
4278 380 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 411 414 417 556 860 1243 411
Size: 3 3 139 304 383 3886
Fragments arranged by size:
3886 383 304 139 3 3
BseYI C'CCAG_C
Cuts at: 2143 2901 3478 3974 2143
Size: 758 577 496 2887
Fragments arranged by size:
2887 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1350 2260 2306 3514 104
Size: 1246 910 46 1208 1308
Fragments arranged by size:
1308 1246 1208 910 46
BsiHKAI G_wGCw'C
Cuts at: 400 462 688 1412 1732 2510 2700 3492 400
Size: 62 226 724 320 778 190 792 1626
Fragments arranged by size:
1626 792 778 724 320 226 190 62
BsmI GAATG_Cn'
Cuts at: 1485 1485
Size: 4718
Bsp1286I G_dGCh'C
Cuts at: 385 400 462 688 1006 1412 1732 2343 2436
Size: 15 62 226 318 406 320 611 93
Cuts at: 2436 2510 2700 2762 3492 385
Size: 74 190 62 730 1611
Fragments arranged by size:
1611 730 611 406 320 318 226 190
93 74 62 62 15
BspEI T'CCGG_A
Cuts at: 620 3938 620
Size: 3318 1400
BspHI T'CATG_A
Cuts at: 3894 3894
Size: 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 716 1584 1768 1982 2284 2665 556
Size: 160 868 184 214 302 381 2609
Fragments arranged by size:
2609 868 381 302 214 184 160
BsrBI CCG'CTC
Cuts at: 50 1012 3010 3064 4548 4713 50
Size: 962 1998 54 1484 165 55
Fragments arranged by size:
1998 1484 962 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1042 2630 1042
Size: 1588 3130
BsrFI r'CCGG_y
Cuts at: 2716 2897 3106 2716
Size: 181 209 4328
Fragments arranged by size:
4328 209 181
BssHII G'CGCG_C
Cuts at: 885 885
Size: 4718
BssSI C'ACGA_G
Cuts at: 2989 3347 2989
Size: 358 4360
BstAPI GCAn_nnn'nTGC
Cuts at: 2095 2095
Size: 4718
BstBI TT'CG_AA
Cuts at: 3079 3079
Size: 4718
BstYI r'GATC_y
Cuts at: 1 941 1543 1743 2210 2233 2568 2814 3826
Size: 940 602 200 467 23 335 246 1012
Cuts at: 3826 3912 3924 4270 1
Size: 86 12 346 449
Fragments arranged by size:
1012 940 602 467 449 346 335 246
200 86 23 12
BstZ17I GTA'TAC
Cuts at: 776 1640 776
Size: 864 3854
Bsu36I CC'TnA_GG
Cuts at: 758 758
Size: 4718
BtgI C'CryG_G
Cuts at: 514 848 983 1205 1380 514
Size: 334 135 222 175 3852
Fragments arranged by size:
3852 334 222 175 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 182 2668 2849 3012 182
Size: 2486 181 163 1888
Fragments arranged by size:
2486 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 1961 1961
Size: 4718
DraI TTT'AAA
Cuts at: 447 1717 447
Size: 1270 3448
DrdI GACnn_nn'nnGTC
Cuts at: 928 2424 3282 928
Size: 1496 858 2364
Fragments arranged by size:
2364 1496 858
EaeI y'GGCC_r
Cuts at: 260 2303 2477 2868 2895 4240 260
Size: 2043 174 391 27 1345 738
Fragments arranged by size:
2043 1345 738 391 174 27
EagI C'GGCC_G
Cuts at: 2303 2303
Size: 4718
EarI CTCTTCn'nnn_
Cuts at: 594 676 846 2741 2951 594
Size: 82 170 1895 210 2361
Fragments arranged by size:
2361 1895 210 170 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 311 2926 3236 3382 4528 311
Size: 2615 310 146 1146 501
Fragments arranged by size:
2615 1146 501 310 146
EcoICRI GAG'CTC
Cuts at: 398 460 686 1730 398
Size: 62 226 1044 3386
Fragments arranged by size:
3386 1044 226 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 700 1068 2543 2975 3722 3990 625
Size: 75 368 1475 432 747 268 1353
Fragments arranged by size:
1475 1353 747 432 368 268 75
EcoNI CCTnn'n_nnAGG
Cuts at: 809 1225 809
Size: 416 4302
EcoO109I rG'GnC_Cy
Cuts at: 921 1857 921
Size: 936 3782
EcoRI G'AATT_C
Cuts at: 1722 1722
Size: 4718
EcoRV GAT'ATC
Cuts at: 273 273
Size: 4718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4718
FspI TGC'GCA
Cuts at: 2499 2499
Size: 4718
HaeII r_GCGC'y
Cuts at: 2152 2400 3422 4401 2152
Size: 248 1022 979 2469
Fragments arranged by size:
2469 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 628 660 813 845 999 1031 1138
Size: 32 193 32 153 32 154 32 107
Cuts at: 1138 1170 2560 2590 2592 2622 403
Size: 32 1390 30 2 30 2499
Fragments arranged by size:
2499 1390 193 154 153 107 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1757 1757
Size: 4718
HindIII A'AGCT_T
Cuts at: 1362 1362
Size: 4718
KasI G'GCGC_C
Cuts at: 2148 2396 2148
Size: 248 4470
KpnI G_GTAC'C
Cuts at: 1738 1738
Size: 4718
MluI A'CGCG_T
Cuts at: 4151 4151
Size: 4718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3389 3573 3389
Size: 184 4534
MscI TGG'CCA
Cuts at: 262 2479 262
Size: 2217 2501
MslI CAynn'nnrTG
Cuts at: 556 722 1022 1210 1415 1594 2707 2834 556
Size: 166 300 188 205 179 1113 127 2440
Fragments arranged by size:
2440 1113 300 205 188 179 166 127
MspA1I CmG'CkG
Cuts at: 266 1829 2143 2503 3516 3761 266
Size: 1563 314 360 1013 245 1223
Fragments arranged by size:
1563 1223 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2899 2899
Size: 4718
NarI GG'CG_CC
Cuts at: 2149 2397 2149
Size: 248 4470
NcoI C'CATG_G
Cuts at: 848 1205 1380 848
Size: 357 175 4186
Fragments arranged by size:
4186 357 175
NgoMIV G'CCGG_C
Cuts at: 2897 2897
Size: 4718
NheI G'CTAG_C
Cuts at: 201 1299 201
Size: 1098 3620
NspI r_CATG'y
Cuts at: 733 1771 2271 3178 733
Size: 1038 500 907 2273
Fragments arranged by size:
2273 1038 907 500
PciI A'CATG_T
Cuts at: 729 3174 729
Size: 2445 2273
PmeI GTTT'AAAC
Cuts at: 1717 1717
Size: 4718
PmlI CAC'GTG
Cuts at: 656 1113 656
Size: 457 4261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4686
PpuMI rG'GwC_Cy
Cuts at: 921 921
Size: 4718
PshAI GACnn'nnGTC
Cuts at: 1461 1461
Size: 4718
PstI C_TGCA'G
Cuts at: 1765 2450 1765
Size: 685 4033
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4718
PvuII CAG'CTG
Cuts at: 266 2143 2503 266
Size: 1877 360 2481
Fragments arranged by size:
2481 1877 360
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 4718
SacI G_AGCT'C
Cuts at: 400 462 688 1732 400
Size: 62 226 1044 3386
Fragments arranged by size:
3386 1044 226 62
SalI G'TCGA_C
Cuts at: 1755 1755
Size: 4718
SapI GCTCTTCn'nnn_
Cuts at: 676 846 2741 2951 676
Size: 170 1895 210 2443
Fragments arranged by size:
2443 1895 210 170
SbfI CC_TGCA'GG
Cuts at: 1765 1765
Size: 4718
ScaI AGT'ACT
Cuts at: 4235 4235
Size: 4718
SfcI C'TryA_G
Cuts at: 33 501 1761 2446 3439 3630 33
Size: 468 1260 685 993 191 1121
Fragments arranged by size:
1260 1121 993 685 468 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 525 525
Size: 4718
SfoI GGC'GCC
Cuts at: 2150 2398 2150
Size: 248 4470
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
SmaI CCC'GGG
Cuts at: 1740 1740
Size: 4718
SmlI C'TyrA_G
Cuts at: 872 1365 1873 2788 3280 3542 872
Size: 493 508 915 492 262 2048
Fragments arranged by size:
2048 915 508 493 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4718
SphI G_CATG'C
Cuts at: 1771 2271 1771
Size: 500 4218
StuI AGG'CCT
Cuts at: 577 577
Size: 4718
StyI C'CwwG_G
Cuts at: 578 848 1205 1380 1852 2206 578
Size: 270 357 175 472 354 3090
Fragments arranged by size:
3090 472 357 354 270 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 450 2301 2434 2893 3102 450
Size: 1851 133 459 209 2066
Fragments arranged by size:
2066 1851 459 209 133
TatI w'GTAC_w
Cuts at: 186 4233 186
Size: 4047 671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1071 1103 4235 4267 1071
Size: 32 3132 32 1522
Fragments arranged by size:
3132 1522 32 32
Tth111I GACn'n_nGTC
Cuts at: 2515 2515
Size: 4718
XbaI T'CTAG_A
Cuts at: 1749 1749
Size: 4718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 2213 1277
Size: 936 3782
XmaI C'CCGG_G
Cuts at: 1738 1738
Size: 4718
XmnI GAAnn'nnTTC
Cuts at: 628 628
Size: 4718
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PmeI PmlI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BsgI BsiWI BsmBI
BsrGI BstEII BstXI ClaI CspCI DraIII FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PflMI PfoI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI