Restriction map for
pF1KB8844
(Circular) (Six-base) MAPSORT of: pF1KB8844.seq Check: 9872 from: 1 to: 4679
pF1KB8844 4679 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 959 1235 959
Size: 276 4403
AatII G_ACGT'C
Cuts at: 1166 1166
Size: 4679
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2936 3683 3951 2504
Size: 432 747 268 3232
Fragments arranged by size:
3232 747 432 268
AflIII A'CryG_T
Cuts at: 1070 1448 2031 3135 4112 1070
Size: 378 583 1104 977 1637
Fragments arranged by size:
1637 1104 977 583 378
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 201 441 960 1984 3551 201
Size: 240 519 1024 1567 1329
Fragments arranged by size:
1567 1329 1024 519 240
ApaI G_GGCC'C
Cuts at: 1265 1265
Size: 4679
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 1683 4032 4288 1683
Size: 2349 256 2074
Fragments arranged by size:
2349 2074 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 159 754 1699 159
Size: 595 945 3139
Fragments arranged by size:
3139 945 595
BamHI G'GATC_C
Cuts at: 1001 1704 1001
Size: 703 3976
BanI G'GyrC_C
Cuts at: 221 285 1399 1695 2109 2357 2392 221
Size: 64 1114 296 414 248 35 2508
Fragments arranged by size:
2508 1114 414 296 248 64 35
BanII G_rGCy'C
Cuts at: 926 998 1214 1265 1608 1693 2723 926
Size: 72 216 51 343 85 1030 2882
Fragments arranged by size:
2882 1030 343 216 85 72 51
BbeI G_GCGC'C
Cuts at: 289 2113 2361 289
Size: 1824 248 2607
Fragments arranged by size:
2607 1824 248
BbvCI CC'TCA_GC
Cuts at: 148 148
Size: 4679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 702 736 891 925 702
Size: 34 155 34 4456
Fragments arranged by size:
4456 155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 679 2577 3338 4679 679
Size: 1898 761 1341 679
Fragments arranged by size:
1898 1341 761 679
BclI T'GATC_A
Cuts at: 1613 2199 1613
Size: 586 4093
BfrBI ATG'CAT
Cuts at: 1539 1539
Size: 4679
BglI GCCn_nnn'nGGC
Cuts at: 311 1276 1339 311
Size: 965 63 3651
Fragments arranged by size:
3651 965 63
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 226 638 1080 1265 1402 2304 2397 3453 226
Size: 412 442 185 137 902 93 1056 1452
Fragments arranged by size:
1452 1056 902 442 412 185 137 93
BmrI ACTGGGnnnn_n'
Cuts at: 876 1891 1972 2307 4451 876
Size: 1015 81 335 2144 1104
Fragments arranged by size:
2144 1104 1015 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1647 3046 1647
Size: 1399 3280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 4679
Bpu10I CC'TnA_GC
Cuts at: 148 148
Size: 4679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 682 1855 2734 3226 3524 652
Size: 30 1173 879 492 298 1807
Fragments arranged by size:
1807 1173 879 492 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 133 931 4383 133
Size: 798 3452 429
Fragments arranged by size:
3452 798 429
BsaAI yAC'GTr
Cuts at: 383 1449 2032 383
Size: 1066 583 3030
Fragments arranged by size:
3030 1066 583
BsaBI GATnn'nnATC
Cuts at: 6 401 2193 4230 6
Size: 395 1792 2037 455
Fragments arranged by size:
2037 1792 455 395
BsaHI Gr'CG_yC
Cuts at: 286 428 1163 1172 2110 2358 3060 4556 286
Size: 142 735 9 938 248 702 1496 409
Fragments arranged by size:
1496 938 735 702 409 248 142 9
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 427 1595 1625 397
Size: 30 1168 30 3451
Fragments arranged by size:
3451 1168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 165
Size: 4679
BseYI C'CCAG_C
Cuts at: 180 214 309 1467 2104 2862 3439 3935 180
Size: 34 95 1158 637 758 577 496 924
Fragments arranged by size:
1158 924 758 637 577 496 95 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 733 796 1396 439
Size: 294 63 600 3722
Fragments arranged by size:
3722 600 294 63
BsiEI CG_ry'CG
Cuts at: 104 1238 2221 2267 3475 104
Size: 1134 983 46 1208 1308
Fragments arranged by size:
1308 1208 1134 983 46
BsiHKAI G_wGCw'C
Cuts at: 761 1361 1445 1693 2471 2661 3453 761
Size: 600 84 248 778 190 792 1987
Fragments arranged by size:
1987 792 778 600 248 190 84
BsmI GAATG_Cn'
Cuts at: 1669 1669
Size: 4679
Bsp1286I G_dGCh'C
Cuts at: 226 638 761 926 998 1080 1214 1265 1361
Size: 412 123 165 72 82 134 51 96
Cuts at: 1361 1402 1445 1608 1693 2304 2397 2471 2661
Size: 41 43 163 85 611 93 74 190
Cuts at: 2661 2723 3453 226
Size: 62 730 1452
Fragments arranged by size:
1452 730 611 412 190 165 163 134
123 96 93 85 82 74 72 62
51 43 41
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 3855 3855
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 530 959 1235 1648 1729 1943 2245 2626 530
Size: 429 276 413 81 214 302 381 2583
Fragments arranged by size:
2583 429 413 381 302 276 214 81
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2591 2591
Size: 4679
BsrFI r'CCGG_y
Cuts at: 236 352 724 2677 2858 3067 236
Size: 116 372 1953 181 209 1848
Fragments arranged by size:
1953 1848 372 209 181 116
BssHII G'CGCG_C
Cuts at: 348 709 348
Size: 361 4318
BssSI C'ACGA_G
Cuts at: 1086 1632 2950 3308 1086
Size: 546 1318 358 2457
Fragments arranged by size:
2457 1318 546 358
BstAPI GCAn_nnn'nTGC
Cuts at: 960 2056 960
Size: 1096 3583
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstYI r'GATC_y
Cuts at: 1 1001 1379 1704 2171 2194 2529 2775 3787
Size: 1000 378 325 467 23 335 246 1012
Cuts at: 3787 3873 3885 4231 1
Size: 86 12 346 449
Fragments arranged by size:
1012 1000 467 449 378 346 335 325
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1477 1477
Size: 4679
BtgI C'CryG_G
Cuts at: 107 579 1337 1403 107
Size: 472 758 66 3383
Fragments arranged by size:
3383 758 472 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1135 1257 1922 1135
Size: 122 665 3892
Fragments arranged by size:
3892 665 122
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DraIII CAC_nnn'GTG
Cuts at: 1389 1389
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3243 2385
Size: 858 3821
EaeI y'GGCC_r
Cuts at: 354 842 1040 1221 1235 1277 2264 2438 2829
Size: 488 198 181 14 42 987 174 391
Cuts at: 2829 2856 4201 354
Size: 27 1345 832
Fragments arranged by size:
1345 987 832 488 391 198 181 174
42 27 14
EagI C'GGCC_G
Cuts at: 1235 2264 1235
Size: 1029 3650
EarI CTCTTCn'nnn_
Cuts at: 580 1349 2702 2912 580
Size: 769 1353 210 2347
Fragments arranged by size:
2347 1353 769 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3197 3343 4489 2887
Size: 310 146 1146 3077
Fragments arranged by size:
3077 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 2504 2936 3683 3951 901
Size: 1603 432 747 268 1629
Fragments arranged by size:
1629 1603 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 280 1195 280
Size: 915 3764
EcoO109I rG'GnC_Cy
Cuts at: 155 185 1261 1262 1818 155
Size: 30 1076 1 556 3016
Fragments arranged by size:
3016 1076 556 30 1
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 798 2698 798
Size: 1900 2779
FspI TGC'GCA
Cuts at: 526 643 2460 526
Size: 117 1817 2745
Fragments arranged by size:
2745 1817 117
FspAI rTGC'GCAy
Cuts at: 643 643
Size: 4679
HaeII r_GCGC'y
Cuts at: 271 289 562 1113 2113 2361 3383 4362 271
Size: 18 273 551 1000 248 1022 979 588
Fragments arranged by size:
1022 1000 979 588 551 273 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1524 1556 2521 2551 2553 2583 1524
Size: 32 965 30 2 30 3620
Fragments arranged by size:
3620 965 32 30 30 2
HincII GTy'rAC
Cuts at: 1718 1718
Size: 4679
KasI G'GCGC_C
Cuts at: 285 2109 2357 285
Size: 1824 248 2607
Fragments arranged by size:
2607 1824 248
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 998 3350 3534 497
Size: 501 2352 184 1642
Fragments arranged by size:
2352 1642 501 184
MscI TGG'CCA
Cuts at: 844 2440 844
Size: 1596 3083
MslI CAynn'nnrTG
Cuts at: 2668 2795 2668
Size: 127 4552
MspA1I CmG'CkG
Cuts at: 218 231 966 1220 1790 2104 2464 3477 3722
Size: 13 735 254 570 314 360 1013 245
Cuts at: 3722 218
Size: 1175
Fragments arranged by size:
1175 1013 735 570 360 314 254 245
13
NaeI GCC'GGC
Cuts at: 354 726 2860 354
Size: 372 2134 2173
Fragments arranged by size:
2173 2134 372
NarI GG'CG_CC
Cuts at: 286 2110 2358 286
Size: 1824 248 2607
Fragments arranged by size:
2607 1824 248
NcoI C'CATG_G
Cuts at: 107 1403 107
Size: 1296 3383
NdeI CA'TA_TG
Cuts at: 608 608
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 352 724 2858 352
Size: 372 2134 2173
Fragments arranged by size:
2173 2134 372
NsiI A_TGCA'T
Cuts at: 1541 1541
Size: 4679
NspI r_CATG'y
Cuts at: 1732 2232 3139 1732
Size: 500 907 3272
Fragments arranged by size:
3272 907 500
PciI A'CATG_T
Cuts at: 3135 3135
Size: 4679
PflMI CCAn_nnn'nTGG
Cuts at: 1389 1389
Size: 4679
PfoI T'CCnGG_A
Cuts at: 650 1166 1453 650
Size: 516 287 3876
Fragments arranged by size:
3876 516 287
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PmlI CAC'GTG
Cuts at: 383 383
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PspOMI G'GGCC_C
Cuts at: 1261 1261
Size: 4679
PspXI vC'TCGA_Gb
Cuts at: 754 754
Size: 4679
PstI C_TGCA'G
Cuts at: 557 956 965 1726 2411 557
Size: 399 9 761 685 2825
Fragments arranged by size:
2825 761 685 399 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 231 966 2104 2464 231
Size: 735 1138 360 2446
Fragments arranged by size:
2446 1138 735 360
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 1349 2702 2912 1349
Size: 1353 210 3116
Fragments arranged by size:
3116 1353 210
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SfcI C'TryA_G
Cuts at: 33 553 952 961 1722 2407 3400 3591 33
Size: 520 399 9 761 685 993 191 1121
Fragments arranged by size:
1121 993 761 685 520 399 191 9
SfoI GGC'GCC
Cuts at: 287 2111 2359 287
Size: 1824 248 2607
Fragments arranged by size:
2607 1824 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 667 697 754 1834 2749 3241 3503 667
Size: 30 57 1080 915 492 262 1843
Fragments arranged by size:
1843 1080 915 492 262 57 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StyI C'CwwG_G
Cuts at: 107 1403 1813 2167 107
Size: 1296 410 354 2619
Fragments arranged by size:
2619 1296 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 115 499 2262 2395 2854 3063 115
Size: 384 1763 133 459 209 1731
Fragments arranged by size:
1763 1731 459 384 209 133
TatI w'GTAC_w
Cuts at: 257 914 4194 257
Size: 657 3280 742
Fragments arranged by size:
3280 742 657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 397 429 940 972 1595 1627 4196
Size: 32 82 32 511 32 623 32 2569
Cuts at: 4196 4228 283
Size: 32 734
Fragments arranged by size:
2569 734 623 511 82 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 769 2476 769
Size: 1707 2972
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1542 2174 1542
Size: 632 4047
XhoI C'TCGA_G
Cuts at: 754 754
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 1462 1462
Size: 4679
ZraI GAC'GTC
Cuts at: 1164 1164
Size: 4679
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BmgBI BmtI BsiWI BsmBI BsrGI BstEII
BstXI BstZ17I ClaI CspCI EcoRV FseI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PpuMI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI