Restriction map for
pF1KB8841
(Circular) (Six-base) MAPSORT of: pF1KB8841.seq Check: 1812 from: 1 to: 4538
pF1KB8841 4538 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1576 1576
Size: 4538
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 755 822 941 2363 2795 3542 3810 211
Size: 544 67 119 1422 432 747 268 939
Fragments arranged by size:
1422 939 747 544 432 268 119 67
AflIII A'CryG_T
Cuts at: 1890 2994 3971 1890
Size: 1104 977 2457
Fragments arranged by size:
2457 1104 977
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AleI CACnn'nnGTG
Cuts at: 1400 1400
Size: 4538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1404 1404
Size: 4538
AlwNI CAG_nnn'CTG
Cuts at: 183 1503 1843 3410 183
Size: 1320 340 1567 1311
Fragments arranged by size:
1567 1320 1311 340
ApaLI G'TGCA_C
Cuts at: 3308 3308
Size: 4538
ApoI r'AATT_y
Cuts at: 158 570 703 1542 3891 4147 158
Size: 412 133 839 2349 256 549
Fragments arranged by size:
2349 839 549 412 256 133
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 467 1342 1558 467
Size: 875 216 3447
Fragments arranged by size:
3447 875 216
BamHI G'GATC_C
Cuts at: 585 1181 1563 585
Size: 596 382 3560
Fragments arranged by size:
3560 596 382
BanI G'GyrC_C
Cuts at: 615 905 1554 1968 2216 2251 615
Size: 290 649 414 248 35 2902
Fragments arranged by size:
2902 649 414 290 248 35
BanII G_rGCy'C
Cuts at: 290 566 1552 2582 290
Size: 276 986 1030 2246
Fragments arranged by size:
2246 1030 986 276
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BciVI GTATCCnnnnn_n'
Cuts at: 923 1041 2436 3197 4538 923
Size: 118 1395 761 1341 923
Fragments arranged by size:
1395 1341 923 761 118
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglI GCCn_nnn'nGGC
Cuts at: 331 1507 331
Size: 1176 3362
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1650 1650
Size: 4538
Bme1580I G_kGCm'C
Cuts at: 908 2163 2256 3312 908
Size: 1255 93 1056 2134
Fragments arranged by size:
2134 1255 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1170 1750 1831 2166 4310 1170
Size: 580 81 335 2144 1398
Fragments arranged by size:
2144 1398 580 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 4538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 406 443 703 757 1027 1033 190
Size: 216 37 260 54 270 6 3695
Fragments arranged by size:
3695 270 260 216 54 37 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 676 1133 1714 2593 3085 3383 155
Size: 521 457 581 879 492 298 1310
Fragments arranged by size:
1310 879 581 521 492 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 1301 2052 4089 6
Size: 1295 751 2037 455
Fragments arranged by size:
2037 1295 751 455
BsaHI Gr'CG_yC
Cuts at: 454 1969 2217 2919 4415 454
Size: 1515 248 702 1496 577
Fragments arranged by size:
1515 1496 702 577 248
BsaWI w'CCGG_w
Cuts at: 1727 2248 2926 3200 3347 3758 1727
Size: 521 678 274 147 411 2507
Fragments arranged by size:
2507 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1025 1055 1025
Size: 30 4508
BseYI C'CCAG_C
Cuts at: 318 405 432 1239 1963 2721 3298 3794 318
Size: 87 27 807 724 758 577 496 1062
Fragments arranged by size:
1062 807 758 724 577 496 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 308 1043 1552 2330 2520 3312 308
Size: 735 509 778 190 792 1534
Fragments arranged by size:
1534 792 778 735 509 190
BsmBI CGTCTCn'nnnn_
Cuts at: 461 461
Size: 4538
Bsp1286I G_dGCh'C
Cuts at: 290 308 566 908 1043 1552 2163 2256 2330
Size: 18 258 342 135 509 611 93 74
Cuts at: 2330 2520 2582 3312 290
Size: 190 62 730 1516
Fragments arranged by size:
1516 730 611 509 342 258 190 135
93 74 62 18
BspEI T'CCGG_A
Cuts at: 3758 3758
Size: 4538
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2830 2884 4368 4533 50
Size: 2780 54 1484 165 55
Fragments arranged by size:
2780 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1024 1142 2450 1024
Size: 118 1308 3112
Fragments arranged by size:
3112 1308 118
BsrFI r'CCGG_y
Cuts at: 2536 2717 2926 2536
Size: 181 209 4148
Fragments arranged by size:
4148 209 181
BssSI C'ACGA_G
Cuts at: 2809 3167 2809
Size: 358 4180
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstXI CCAn_nnnn'nTGG
Cuts at: 326 413 326
Size: 87 4451
BstYI r'GATC_y
Cuts at: 1 215 384 500 585 1181 1563 2030 2053
Size: 214 169 116 85 596 382 467 23
Cuts at: 2053 2388 2634 3646 3732 3744 4090 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 596 467 449 382 346 335 246
214 169 116 86 85 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4538
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 184 1781 184
Size: 1597 2941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 160 195 160
Size: 35 4503
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DraIII CAC_nnn'GTG
Cuts at: 1271 1271
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 1346 2244 3102 1346
Size: 898 858 2782
Fragments arranged by size:
2782 898 858
EaeI y'GGCC_r
Cuts at: 2123 2297 2688 2715 4060 2123
Size: 174 391 27 1345 2601
Fragments arranged by size:
2601 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 1280 2561 2771 1280
Size: 1281 210 3047
Fragments arranged by size:
3047 1281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3056 3202 4348 2746
Size: 310 146 1146 2936
Fragments arranged by size:
2936 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1550 1550
Size: 4538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 211 406 443 703 755 757 822 941
Size: 21 195 37 260 52 2 65 119
Cuts at: 941 1027 1033 2363 2795 3542 3810 190
Size: 86 6 1330 432 747 268 918
Fragments arranged by size:
1330 918 747 432 268 260 195 119
86 65 52 37 21 6 2
EcoO109I rG'GnC_Cy
Cuts at: 429 526 786 1500 1677 429
Size: 97 260 714 177 3290
Fragments arranged by size:
3290 714 260 177 97
EcoRI G'AATT_C
Cuts at: 570 703 1542 570
Size: 133 839 3566
Fragments arranged by size:
3566 839 133
EcoRV GAT'ATC
Cuts at: 996 996
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1216 2557 1216
Size: 1341 3197
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 1972 2220 3242 4221 1972
Size: 248 1022 979 2289
Fragments arranged by size:
2289 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1210 1242 2380 2410 2412 2442 1210
Size: 32 1138 30 2 30 3306
Fragments arranged by size:
3306 1138 32 30 30 2
HincII GTy'rAC
Cuts at: 1577 1577
Size: 4538
HindIII A'AGCT_T
Cuts at: 856 856
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MfeI C'AATT_G
Cuts at: 948 948
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3393 3209
Size: 184 4354
MscI TGG'CCA
Cuts at: 2299 2299
Size: 4538
MslI CAynn'nnrTG
Cuts at: 1036 1400 2527 2654 1036
Size: 364 1127 127 2920
Fragments arranged by size:
2920 1127 364 127
MspA1I CmG'CkG
Cuts at: 879 1357 1649 1963 2323 3336 3581 879
Size: 478 292 314 360 1013 245 1836
Fragments arranged by size:
1836 1013 478 360 314 292 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 107 107
Size: 4538
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 1591 2091 2998 1591
Size: 500 907 3131
Fragments arranged by size:
3131 907 500
PasI CC'CwG_GG
Cuts at: 395 395
Size: 4538
PciI A'CATG_T
Cuts at: 2994 2994
Size: 4538
PfoI T'CCnGG_A
Cuts at: 422 1043 422
Size: 621 3917
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PstI C_TGCA'G
Cuts at: 639 1329 1508 1585 2270 639
Size: 690 179 77 685 2907
Fragments arranged by size:
2907 690 685 179 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 879 1357 1963 2323 879
Size: 478 606 360 3094
Fragments arranged by size:
3094 606 478 360
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 1552 1552
Size: 4538
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1508 1585 1508
Size: 77 4461
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 635 806 1325 1504 1581 2266 3259 3450
Size: 602 171 519 179 77 685 993 191
Cuts at: 3450 33
Size: 1121
Fragments arranged by size:
1121 993 685 602 519 191 179 171
77
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 469 1560 469
Size: 1091 3447
SmlI C'TyrA_G
Cuts at: 170 655 1148 1693 2608 3100 3362 170
Size: 485 493 545 915 492 262 1346
Fragments arranged by size:
1346 915 545 493 492 485 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
SspI AAT'ATT
Cuts at: 597 597
Size: 4538
StuI AGG'CCT
Cuts at: 1525 1525
Size: 4538
StyI C'CwwG_G
Cuts at: 107 245 281 1107 1672 2026 107
Size: 138 36 826 565 354 2619
Fragments arranged by size:
2619 826 565 354 138 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 2121 2254 2713 2922 553
Size: 1568 133 459 209 2169
Fragments arranged by size:
2169 1568 459 209 133
TatI w'GTAC_w
Cuts at: 4053 4053
Size: 4538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4506
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2033 2033
Size: 4538
XmaI C'CCGG_G
Cuts at: 467 1558 467
Size: 1091 3447
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoNI FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI