Restriction map for
pF1KB8840
(Circular) (Six-base) MAPSORT of: pF1KB8840.seq Check: 6875 from: 1 to: 4514
pF1KB8840 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 614 1552 614
Size: 938 3576
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1399 2339 2771 3518 3786 565
Size: 834 940 432 747 268 1293
Fragments arranged by size:
1293 940 834 747 432 268
AflII C'TTAA_G
Cuts at: 661 661
Size: 4514
AflIII A'CryG_T
Cuts at: 1866 2970 3947 1866
Size: 1104 977 2433
Fragments arranged by size:
2433 1104 977
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 389 572 389
Size: 183 4331
AlwNI CAG_nnn'CTG
Cuts at: 270 708 1819 3386 270
Size: 438 1111 1567 1398
Fragments arranged by size:
1567 1398 1111 438
ApaI G_GGCC'C
Cuts at: 814 1334 814
Size: 520 3994
ApaLI G'TGCA_C
Cuts at: 145 3284 145
Size: 3139 1375
ApoI r'AATT_y
Cuts at: 1518 3867 4123 1518
Size: 2349 256 1909
Fragments arranged by size:
2349 1909 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 569 1034 1534 569
Size: 465 500 3549
Fragments arranged by size:
3549 500 465
BamHI G'GATC_C
Cuts at: 1445 1539 1445
Size: 94 4420
BanI G'GyrC_C
Cuts at: 685 866 937 1530 1944 2192 2227 685
Size: 181 71 593 414 248 35 2972
Fragments arranged by size:
2972 593 414 248 181 71 35
BanII G_rGCy'C
Cuts at: 541 814 928 1334 1440 1528 2558 541
Size: 273 114 406 106 88 1030 2497
Fragments arranged by size:
2497 1030 406 273 114 106 88
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BbvCI CC'TCA_GC
Cuts at: 1286 1286
Size: 4514
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3173 4514 2412
Size: 761 1341 2412
Fragments arranged by size:
2412 1341 761
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglI GCCn_nnn'nGGC
Cuts at: 241 1003 1171 241
Size: 762 168 3584
Fragments arranged by size:
3584 762 168
BglII A'GATC_T
Cuts at: 1299 2029 4066 1299
Size: 730 2037 1747
Fragments arranged by size:
2037 1747 730
BlpI GC'TnA_GC
Cuts at: 535 1439 1626 535
Size: 904 187 3423
Fragments arranged by size:
3423 904 187
Bme1580I G_kGCm'C
Cuts at: 149 378 814 869 940 993 1334 2139 2232
Size: 229 436 55 71 53 341 805 93
Cuts at: 2232 3288 149
Size: 1056 1375
Fragments arranged by size:
1375 1056 805 436 341 229 93 71
55 53
BmrI ACTGGGnnnn_n'
Cuts at: 896 902 1726 1807 2142 4286 896
Size: 6 824 81 335 2144 1124
Fragments arranged by size:
2144 1124 824 335 81 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1465 386
Size: 1079 3435
Bpu10I CC'TnA_GC
Cuts at: 1028 1067 1079 1217 1286 1028
Size: 39 12 138 69 4256
Fragments arranged by size:
4256 138 69 39 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1690 2569 3061 3359 664
Size: 1026 879 492 298 1819
Fragments arranged by size:
1819 1026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3176 3323 3734 1703
Size: 521 678 274 147 411 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 384 414 384
Size: 30 4484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 722 1303 722
Size: 581 3933
BseYI C'CCAG_C
Cuts at: 245 1181 1939 2697 3274 3770 245
Size: 936 758 758 577 496 989
Fragments arranged by size:
989 936 758 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 596
Size: 4514
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3310 104
Size: 1952 46 1208 1308
Fragments arranged by size:
1952 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 414 541 1528 2306 2496 3288 149
Size: 265 127 987 778 190 792 1375
Fragments arranged by size:
1375 987 792 778 265 190 127
Bsp1286I G_dGCh'C
Cuts at: 149 378 414 541 814 869 928 940 993
Size: 229 36 127 273 55 59 12 53
Cuts at: 993 1334 1440 1528 2139 2232 2306 2496 2558
Size: 341 106 88 611 93 74 190 62
Cuts at: 2558 3288 149
Size: 730 1375
Fragments arranged by size:
1375 730 611 341 273 229 190 127
106 93 88 74 62 59 55 53
36 12
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 3690 3690
Size: 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 430 536 1564 1778 2080 2461 430
Size: 106 1028 214 302 381 2483
Fragments arranged by size:
2483 1028 381 302 214 106
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1486 2426 1486
Size: 940 3574
BsrFI r'CCGG_y
Cuts at: 2512 2693 2902 2512
Size: 181 209 4124
Fragments arranged by size:
4124 209 181
BssSI C'ACGA_G
Cuts at: 371 986 2785 3143 371
Size: 615 1799 358 1742
Fragments arranged by size:
1799 1742 615 358
BstAPI GCAn_nnn'nTGC
Cuts at: 270 1891 270
Size: 1621 2893
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1113 114
Size: 999 3515
BstYI r'GATC_y
Cuts at: 1 225 1210 1299 1445 1539 2006 2029 2364
Size: 224 985 89 146 94 467 23 335
Cuts at: 2364 2610 3622 3708 3720 4066 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 985 467 449 346 335 246 224
146 94 89 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1343 1343
Size: 4514
BtgI C'CryG_G
Cuts at: 167 167
Size: 4514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 196 312 451 587 706 1757 196
Size: 116 139 136 119 1051 2953
Fragments arranged by size:
2953 1051 139 136 119 116
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 482 974 2099 2273 2664 2691 4036 482
Size: 492 1125 174 391 27 1345 960
Fragments arranged by size:
1345 1125 960 492 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 185 1267 1309 2537 2747 185
Size: 1082 42 1228 210 1952
Fragments arranged by size:
1952 1228 1082 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3032 3178 4324 2722
Size: 310 146 1146 2912
Fragments arranged by size:
2912 1146 310 146
EcoICRI GAG'CTC
Cuts at: 539 1526 539
Size: 987 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 565 1399 1465 2339 2771 3518 3786 386
Size: 179 834 66 874 432 747 268 1114
Fragments arranged by size:
1114 874 834 747 432 268 179 66
EcoNI CCTnn'n_nnAGG
Cuts at: 190 190
Size: 4514
EcoO109I rG'GnC_Cy
Cuts at: 183 267 648 810 811 835 896 1331 1357
Size: 84 381 162 1 24 61 435 26
Cuts at: 1357 1653 183
Size: 296 3044
Fragments arranged by size:
3044 435 381 296 162 84 61 26
24 1
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 304 2295 304
Size: 1991 2523
HaeII r_GCGC'y
Cuts at: 1948 2196 3218 4197 1948
Size: 248 1022 979 2265
Fragments arranged by size:
2265 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 1393 1425 2356 2386 2388 2418 210
Size: 32 1151 32 931 30 2 30 2306
Fragments arranged by size:
2306 1151 931 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 312 3185 3369 312
Size: 2873 184 1457
Fragments arranged by size:
2873 1457 184
MscI TGG'CCA
Cuts at: 484 976 2275 484
Size: 492 1299 2723
Fragments arranged by size:
2723 1299 492
MslI CAynn'nnrTG
Cuts at: 112 2503 2630 112
Size: 2391 127 1996
Fragments arranged by size:
2391 1996 127
MspA1I CmG'CkG
Cuts at: 1434 1443 1625 1939 2299 3312 3557 1434
Size: 9 182 314 360 1013 245 2391
Fragments arranged by size:
2391 1013 360 314 245 182 9
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 130 517 1567 2067 2974 130
Size: 387 1050 500 907 1670
Fragments arranged by size:
1670 1050 907 500 387
PasI CC'CwG_GG
Cuts at: 838 933 1187 838
Size: 95 254 4165
Fragments arranged by size:
4165 254 95
PciI A'CATG_T
Cuts at: 2970 2970
Size: 4514
PflMI CCAn_nnn'nTGG
Cuts at: 252 252
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 896 896
Size: 4514
PspOMI G'GGCC_C
Cuts at: 810 1330 810
Size: 520 3994
PstI C_TGCA'G
Cuts at: 266 441 713 1561 2246 266
Size: 175 272 848 685 2534
Fragments arranged by size:
2534 848 685 272 175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 541 1528 541
Size: 987 3527
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SanDI GG'GwC_CC
Cuts at: 896 896
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 266 441 1561 266
Size: 175 1120 3219
Fragments arranged by size:
3219 1120 175
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 262 324 437 709 1557 2242 3235 3426
Size: 229 62 113 272 848 685 993 191
Cuts at: 3426 33
Size: 1121
Fragments arranged by size:
1121 993 848 685 272 229 191 113
62
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 571 1036 1536 571
Size: 465 500 3549
Fragments arranged by size:
3549 500 465
SmlI C'TyrA_G
Cuts at: 643 661 1669 2584 3076 3338 643
Size: 18 1008 915 492 262 1819
Fragments arranged by size:
1819 1008 915 492 262 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 517 1567 2067 517
Size: 1050 500 2964
Fragments arranged by size:
2964 1050 500
SrfI GCCC'GGGC
Cuts at: 1036 1036
Size: 4514
StyI C'CwwG_G
Cuts at: 956 998 1019 1040 1046 1100 1127 1133 1145
Size: 42 21 21 6 54 27 6 12
Cuts at: 1145 1160 1166 1648 2002 956
Size: 15 6 482 354 3468
Fragments arranged by size:
3468 482 354 54 42 27 21 21
15 12 6 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 673 2097 2230 2689 2898 610
Size: 63 1424 133 459 209 2226
Fragments arranged by size:
2226 1424 459 209 133 63
TatI w'GTAC_w
Cuts at: 4029 4029
Size: 4514
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 384 416 441 473 4031 4063 327
Size: 32 25 32 25 32 3558 32 778
Fragments arranged by size:
3558 778 32 32 32 32 25 25
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 678 1053 1167 2009 114
Size: 564 375 114 842 2619
Fragments arranged by size:
2619 842 564 375 114
XmaI C'CCGG_G
Cuts at: 569 1034 1534 569
Size: 465 500 3549
Fragments arranged by size:
3549 500 465
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI