Restriction map for
pF1KB8836
(Circular) (Six-base) MAPSORT of: pF1KB8836.seq Check: 4725 from: 1 to: 4496
pF1KB8836 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 843 1431 2321 2753 3500 3768 423
Size: 420 588 890 432 747 268 1151
Fragments arranged by size:
1151 890 747 588 432 420 268
AflII C'TTAA_G
Cuts at: 460 460
Size: 4496
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 1801 3368 1801
Size: 1567 2929
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 256 363 557 699 789 1119 1145 1313 1371
Size: 107 194 142 90 330 26 168 58
Cuts at: 1371 1500 3849 4105 256
Size: 129 2349 256 647
Fragments arranged by size:
2349 647 330 256 194 168 142 129
107 90 58 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 451 955 1510 2540 451
Size: 504 555 1030 2407
Fragments arranged by size:
2407 1030 555 504
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 1344 2016 1344
Size: 672 3824
BfrBI ATG'CAT
Cuts at: 873 873
Size: 4496
BglII A'GATC_T
Cuts at: 407 2011 4048 407
Size: 1604 2037 855
Fragments arranged by size:
2037 1604 855
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3270 2121
Size: 93 1056 3347
Fragments arranged by size:
3347 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 491 1708 1789 2124 4268 491
Size: 1217 81 335 2144 719
Fragments arranged by size:
2144 1217 719 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 974 2863 974
Size: 1889 2607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 671 755 923 1007 1091 1175 1259 587
Size: 84 84 168 84 84 84 84 3824
Fragments arranged by size:
3824 168 84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 188 2010 4047 6
Size: 182 1822 2037 455
Fragments arranged by size:
2037 1822 455 182
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1921 2679 3256 3752 1921
Size: 758 577 496 2665
Fragments arranged by size:
2665 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 447
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 451 955 1510 2288 2478 3270 451
Size: 504 555 778 190 792 1677
Fragments arranged by size:
1677 792 778 555 504 190
BsmI GAATG_Cn'
Cuts at: 129 305 1276 1438 129
Size: 176 971 162 3187
Fragments arranged by size:
3187 971 176 162
Bsp1286I G_dGCh'C
Cuts at: 451 955 1510 2121 2214 2288 2478 2540 3270
Size: 504 555 611 93 74 190 62 730
Cuts at: 3270 451
Size: 1677
Fragments arranged by size:
1677 730 611 555 504 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1266 2408 1266
Size: 1142 3354
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BsrGI T'GTAC_A
Cuts at: 546 882 546
Size: 336 4160
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstYI r'GATC_y
Cuts at: 1 407 1521 1988 2011 2346 2592 3604 3690
Size: 406 1114 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1114 1012 467 449 406 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1739 1739
Size: 4496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1248 1283 1248
Size: 35 4461
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 953 1508 449
Size: 504 555 3437
Fragments arranged by size:
3437 555 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 587 671 755 843 923 1007 1091 1175
Size: 164 84 84 88 80 84 84 84
Cuts at: 1175 1259 1431 2321 2753 3500 3768 423
Size: 84 172 890 432 747 268 1151
Fragments arranged by size:
1151 890 747 432 268 172 164 88
84 84 84 84 84 84 80
EcoO109I rG'GnC_Cy
Cuts at: 323 1635 323
Size: 1312 3184
EcoRI G'AATT_C
Cuts at: 557 699 1119 1145 1313 1371 1500 557
Size: 142 420 26 168 58 129 3553
Fragments arranged by size:
3553 420 168 142 129 58 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 2515 240
Size: 2275 2221
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3200 4179 1930
Size: 248 1022 979 2247
Fragments arranged by size:
2247 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 606 638 786 818 2338 2368 2370 2400 606
Size: 32 148 32 1520 30 2 30 2702
Fragments arranged by size:
2702 1520 148 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
HindIII A'AGCT_T
Cuts at: 770 1106 770
Size: 336 4160
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 232 310 1322 2485 2612 232
Size: 78 1012 1163 127 2116
Fragments arranged by size:
2116 1163 1012 127 78
MspA1I CmG'CkG
Cuts at: 1607 1921 2281 3294 3539 1607
Size: 314 360 1013 245 2564
Fragments arranged by size:
2564 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NsiI A_TGCA'T
Cuts at: 875 875
Size: 4496
NspI r_CATG'y
Cuts at: 873 1549 2049 2956 873
Size: 676 500 907 2413
Fragments arranged by size:
2413 907 676 500
PacI TTA_AT'TAA
Cuts at: 377 377
Size: 4496
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 451 955 1510 451
Size: 504 555 3437
Fragments arranged by size:
3437 555 504
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1539 2224 3217 3408 33
Size: 1506 685 993 191 1121
Fragments arranged by size:
1506 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 460 1651 2566 3058 3320 460
Size: 1191 915 492 262 1636
Fragments arranged by size:
1636 1191 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 873 1549 2049 873
Size: 676 500 3320
Fragments arranged by size:
3320 676 500
StyI C'CwwG_G
Cuts at: 107 1630 1984 107
Size: 1523 354 2619
Fragments arranged by size:
2619 1523 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2079 2212 2671 2880 2079
Size: 133 459 209 3695
Fragments arranged by size:
3695 459 209 133
TatI w'GTAC_w
Cuts at: 546 882 1036 1456 4011 546
Size: 336 154 420 2555 1031
Fragments arranged by size:
2555 1031 420 336 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 520 725 520
Size: 205 4291
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PacI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI