Restriction map for
pF1KB8835
(Circular) (Six-base) MAPSORT of: pF1KB8835.seq Check: 3819 from: 1 to: 4490
pF1KB8835 4490 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1528 1528
Size: 4490
Acc65I G'GTAC_C
Cuts at: 1506 1506
Size: 4490
AclI AA'CG_TT
Cuts at: 4330 4330
Size: 4490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2747 3494 3762 2315
Size: 432 747 268 3043
Fragments arranged by size:
3043 747 432 268
AflIII A'CryG_T
Cuts at: 719 1842 2946 3923 719
Size: 1123 1104 977 1286
Fragments arranged by size:
1286 1123 1104 977
AgeI A'CCGG_T
Cuts at: 2878 2878
Size: 4490
AleI CACnn'nnGTG
Cuts at: 1269 1269
Size: 4490
AlwNI CAG_nnn'CTG
Cuts at: 294 1795 3362 294
Size: 1501 1567 1422
Fragments arranged by size:
1567 1501 1422
ApaLI G'TGCA_C
Cuts at: 338 1110 3260 338
Size: 772 2150 1568
Fragments arranged by size:
2150 1568 772
ApoI r'AATT_y
Cuts at: 388 1494 3843 4099 388
Size: 1106 2349 256 779
Fragments arranged by size:
2349 1106 779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
AvaI C'yCGr_G
Cuts at: 981 1019 1335 1510 981
Size: 38 316 175 3961
Fragments arranged by size:
3961 316 175 38
BamHI G'GATC_C
Cuts at: 1515 1515
Size: 4490
BanI G'GyrC_C
Cuts at: 379 1403 1506 1920 2168 2203 379
Size: 1024 103 414 248 35 2666
Fragments arranged by size:
2666 1024 414 248 103 35
BanII G_rGCy'C
Cuts at: 494 1504 2534 494
Size: 1010 1030 2450
Fragments arranged by size:
2450 1030 1010
BbeI G_GCGC'C
Cuts at: 383 1924 2172 383
Size: 1541 248 2701
Fragments arranged by size:
2701 1541 248
BbsI GAAGACnn'nnnn_
Cuts at: 677 1146 677
Size: 469 4021
BbvCI CC'TCA_GC
Cuts at: 475 475
Size: 4490
BciVI GTATCCnnnnn_n'
Cuts at: 2388 3149 4490 2388
Size: 761 1341 2388
Fragments arranged by size:
2388 1341 761
BclI T'GATC_A
Cuts at: 2010 2010
Size: 4490
BglI GCCn_nnn'nGGC
Cuts at: 1024 1459 1024
Size: 435 4055
BglII A'GATC_T
Cuts at: 2005 4042 2005
Size: 2037 2453
BlpI GC'TnA_GC
Cuts at: 1602 1602
Size: 4490
Bme1580I G_kGCm'C
Cuts at: 342 1114 1123 1192 2115 2208 3264 342
Size: 772 9 69 923 93 1056 1568
Fragments arranged by size:
1568 1056 923 772 93 69 9
BmgBI CAC'GTC
Cuts at: 210 720 1371 210
Size: 510 651 3329
Fragments arranged by size:
3329 651 510
BmrI ACTGGGnnnn_n'
Cuts at: 1702 1783 2118 4262 1702
Size: 81 335 2144 1930
Fragments arranged by size:
2144 1930 335 81
BmtI G_CTAG'C
Cuts at: 533 533
Size: 4490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 673 778 806 1468 557
Size: 116 105 28 662 3579
Fragments arranged by size:
3579 662 116 105 28
Bpu10I CC'TnA_GC
Cuts at: 475 475
Size: 4490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1393 1666 2545 3037 3335 217
Size: 1176 273 879 492 298 1372
Fragments arranged by size:
1372 1176 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 327 4194 327
Size: 3867 623
BsaAI yAC'GTr
Cuts at: 450 1843 450
Size: 1393 3097
BsaBI GATnn'nnATC
Cuts at: 6 2004 4041 6
Size: 1998 2037 455
Fragments arranged by size:
2037 1998 455
BsaHI Gr'CG_yC
Cuts at: 380 1921 2169 2871 4367 380
Size: 1541 248 702 1496 503
Fragments arranged by size:
1541 1496 702 503 248
BsaWI w'CCGG_w
Cuts at: 1679 2200 2878 3152 3299 3710 1679
Size: 521 678 274 147 411 2459
Fragments arranged by size:
2459 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 806 836 1342 1372 806
Size: 30 506 30 3924
Fragments arranged by size:
3924 506 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 352 556 608 208
Size: 144 204 52 4090
Fragments arranged by size:
4090 204 144 52
BseYI C'CCAG_C
Cuts at: 304 865 1915 2673 3250 3746 304
Size: 561 1050 758 577 496 1048
Fragments arranged by size:
1050 1048 758 577 561 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1315
Size: 4490
BsiEI CG_ry'CG
Cuts at: 104 1436 2032 2078 3286 104
Size: 1332 596 46 1208 1308
Fragments arranged by size:
1332 1308 1208 596 46
BsiHKAI G_wGCw'C
Cuts at: 342 449 1114 1504 2282 2472 3264 342
Size: 107 665 390 778 190 792 1568
Fragments arranged by size:
1568 792 778 665 390 190 107
BsmI GAATG_Cn'
Cuts at: 474 474
Size: 4490
Bsp1286I G_dGCh'C
Cuts at: 342 449 494 1114 1123 1192 1504 2115 2208
Size: 107 45 620 9 69 312 611 93
Cuts at: 2208 2282 2472 2534 3264 342
Size: 74 190 62 730 1568
Fragments arranged by size:
1568 730 620 611 312 190 107 93
74 69 62 45 9
BspEI T'CCGG_A
Cuts at: 3710 3710
Size: 4490
BspHI T'CATG_A
Cuts at: 3666 3666
Size: 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 443 1337 1540 1754 2056 2437 309
Size: 134 894 203 214 302 381 2362
Fragments arranged by size:
2362 894 381 302 214 203 134
BsrBI CCG'CTC
Cuts at: 50 198 2782 2836 4320 4485 50
Size: 148 2584 54 1484 165 55
Fragments arranged by size:
2584 1484 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 2402 2402
Size: 4490
BsrFI r'CCGG_y
Cuts at: 770 2488 2669 2878 770
Size: 1718 181 209 2382
Fragments arranged by size:
2382 1718 209 181
BssSI C'ACGA_G
Cuts at: 151 1269 2761 3119 151
Size: 1118 1492 358 1522
Fragments arranged by size:
1522 1492 1118 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1118 1867 1118
Size: 749 3741
BstBI TT'CG_AA
Cuts at: 2851 2851
Size: 4490
BstYI r'GATC_y
Cuts at: 1 1515 1982 2005 2340 2586 3598 3684 3696
Size: 1514 467 23 335 246 1012 86 12
Cuts at: 3696 4042 1
Size: 346 449
Fragments arranged by size:
1514 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 821 1010 1430 107
Size: 714 189 420 3167
Fragments arranged by size:
3167 714 420 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2440 2621 2784 2440
Size: 181 163 4146
Fragments arranged by size:
4146 181 163
BtsI GCAGTG_nn'
Cuts at: 1733 1733
Size: 4490
DraI TTT'AAA
Cuts at: 1489 1489
Size: 4490
DraIII CAC_nnn'GTG
Cuts at: 453 1118 453
Size: 665 3825
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3054 2196
Size: 858 3632
EaeI y'GGCC_r
Cuts at: 147 221 260 350 640 828 1007 1433 1460
Size: 74 39 90 290 188 179 426 27
Cuts at: 1460 2075 2249 2640 2667 4012 147
Size: 615 174 391 27 1345 625
Fragments arranged by size:
1345 625 615 426 391 290 188 179
174 90 74 39 27 27
EagI C'GGCC_G
Cuts at: 1433 2075 1433
Size: 642 3848
EarI CTCTTCn'nnn_
Cuts at: 689 2513 2723 689
Size: 1824 210 2456
Fragments arranged by size:
2456 1824 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2698 3008 3154 4300 2698
Size: 310 146 1146 2888
Fragments arranged by size:
2888 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1502 1502
Size: 4490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 673 778 806 1468 2315 2747 3494 3762
Size: 116 105 28 662 847 432 747 268
Cuts at: 3762 557
Size: 1285
Fragments arranged by size:
1285 847 747 662 432 268 116 105
28
EcoNI CCTnn'n_nnAGG
Cuts at: 508 508
Size: 4490
EcoO109I rG'GnC_Cy
Cuts at: 581 936 1060 1629 581
Size: 355 124 569 3442
Fragments arranged by size:
3442 569 355 124
EcoRI G'AATT_C
Cuts at: 388 1494 388
Size: 1106 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 1170 2509 273
Size: 897 1339 2254
Fragments arranged by size:
2254 1339 897
FspI TGC'GCA
Cuts at: 2271 2271
Size: 4490
HaeII r_GCGC'y
Cuts at: 383 554 1924 2172 3194 4173 383
Size: 171 1370 248 1022 979 700
Fragments arranged by size:
1370 1022 979 700 248 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 2332 2362 2364 2394 706
Size: 32 1594 30 2 30 2802
Fragments arranged by size:
2802 1594 32 30 30 2
HincII GTy'rAC
Cuts at: 411 1529 411
Size: 1118 3372
KasI G'GCGC_C
Cuts at: 379 1920 2168 379
Size: 1541 248 2701
Fragments arranged by size:
2701 1541 248
KpnI G_GTAC'C
Cuts at: 1510 1510
Size: 4490
MluI A'CGCG_T
Cuts at: 3923 3923
Size: 4490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 896 3161 3345 896
Size: 2265 184 2041
Fragments arranged by size:
2265 2041 184
MscI TGG'CCA
Cuts at: 830 1009 2251 830
Size: 179 1242 3069
Fragments arranged by size:
3069 1242 179
MslI CAynn'nnrTG
Cuts at: 1183 1243 1253 1269 2479 2606 1183
Size: 60 10 16 1210 127 3067
Fragments arranged by size:
3067 1210 127 60 16 10
MspA1I CmG'CkG
Cuts at: 141 1003 1355 1601 1915 2275 3288 3533 141
Size: 862 352 246 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 862 360 352 314 246 245
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 4490
NarI GG'CG_CC
Cuts at: 380 1921 2169 380
Size: 1541 248 2701
Fragments arranged by size:
2701 1541 248
NcoI C'CATG_G
Cuts at: 107 1010 107
Size: 903 3587
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 4490
NheI G'CTAG_C
Cuts at: 529 529
Size: 4490
NspI r_CATG'y
Cuts at: 1164 1543 2043 2950 1164
Size: 379 500 907 2704
Fragments arranged by size:
2704 907 500 379
PciI A'CATG_T
Cuts at: 2946 2946
Size: 4490
PflMI CCAn_nnn'nTGG
Cuts at: 1140 1140
Size: 4490
PfoI T'CCnGG_A
Cuts at: 951 951
Size: 4490
PmeI GTTT'AAAC
Cuts at: 1489 1489
Size: 4490
PmlI CAC'GTG
Cuts at: 450 450
Size: 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 216 292 324 3629 3661 184
Size: 32 76 32 3305 32 1013
Fragments arranged by size:
3305 1013 76 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 581 1060 581
Size: 479 4011
PstI C_TGCA'G
Cuts at: 320 440 1537 2222 320
Size: 120 1097 685 2588
Fragments arranged by size:
2588 1097 685 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4490
PvuII CAG'CTG
Cuts at: 141 1915 2275 141
Size: 1774 360 2356
Fragments arranged by size:
2356 1774 360
RsrII CG'GwC_CG
Cuts at: 2685 2685
Size: 4490
SacI G_AGCT'C
Cuts at: 1504 1504
Size: 4490
SalI G'TCGA_C
Cuts at: 1527 1527
Size: 4490
SanDI GG'GwC_CC
Cuts at: 1060 1060
Size: 4490
SapI GCTCTTCn'nnn_
Cuts at: 2513 2723 2513
Size: 210 4280
SbfI CC_TGCA'GG
Cuts at: 1537 1537
Size: 4490
ScaI AGT'ACT
Cuts at: 4007 4007
Size: 4490
SfcI C'TryA_G
Cuts at: 33 316 436 872 906 1533 2218 3211 3402
Size: 283 120 436 34 627 685 993 191
Cuts at: 3402 33
Size: 1121
Fragments arranged by size:
1121 993 685 627 436 283 191 120
34
SfiI GGCCn_nnn'nGGCC
Cuts at: 1459 1459
Size: 4490
SfoI GGC'GCC
Cuts at: 381 1922 2170 381
Size: 1541 248 2701
Fragments arranged by size:
2701 1541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
SmaI CCC'GGG
Cuts at: 1021 1512 1021
Size: 491 3999
SmlI C'TyrA_G
Cuts at: 232 1408 1645 2560 3052 3314 232
Size: 1176 237 915 492 262 1408
Fragments arranged by size:
1408 1176 915 492 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4490
SphI G_CATG'C
Cuts at: 1543 2043 1543
Size: 500 3990
StuI AGG'CCT
Cuts at: 503 791 901 503
Size: 288 110 4092
Fragments arranged by size:
4092 288 110
StyI C'CwwG_G
Cuts at: 107 383 834 1010 1624 1978 107
Size: 276 451 176 614 354 2619
Fragments arranged by size:
2619 614 451 354 276 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 814 2073 2206 2665 2874 814
Size: 1259 133 459 209 2430
Fragments arranged by size:
2430 1259 459 209 133
TatI w'GTAC_w
Cuts at: 4005 4005
Size: 4490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 806 838 4007 4039 806
Size: 32 3169 32 1257
Fragments arranged by size:
3169 1257 32 32
Tth111I GACn'n_nGTC
Cuts at: 406 2287 406
Size: 1881 2609
XbaI T'CTAG_A
Cuts at: 1521 1521
Size: 4490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 1985 984
Size: 1001 3489
XmaI C'CCGG_G
Cuts at: 1019 1510 1019
Size: 491 3999
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI