Restriction map for
pF1KB8833
(Circular) (Six-base) MAPSORT of: pF1KB8833.seq Check: 9714 from: 1 to: 4472
pF1KB8833 4472 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 179 238 1510 179
Size: 59 1272 3141
Fragments arranged by size:
3141 1272 59
Acc65I G'GTAC_C
Cuts at: 496 1488 496
Size: 992 3480
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 472 2297 2729 3476 3744 301
Size: 171 1825 432 747 268 1029
Fragments arranged by size:
1825 1029 747 432 268 171
AflIII A'CryG_T
Cuts at: 1824 2928 3905 1824
Size: 1104 977 2391
Fragments arranged by size:
2391 1104 977
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AhdI GACnn_n'nnGTC
Cuts at: 234 234
Size: 4472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 747 779 747
Size: 32 4440
AlwNI CAG_nnn'CTG
Cuts at: 304 1777 3344 304
Size: 1473 1567 1432
Fragments arranged by size:
1567 1473 1432
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 1339 1476 3825 4081 1339
Size: 137 2349 256 1730
Fragments arranged by size:
2349 1730 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 119 358 1492 119
Size: 239 1134 3099
Fragments arranged by size:
3099 1134 239
BamHI G'GATC_C
Cuts at: 397 1497 397
Size: 1100 3372
BanI G'GyrC_C
Cuts at: 163 496 1488 1902 2150 2185 163
Size: 333 992 414 248 35 2450
Fragments arranged by size:
2450 992 414 333 248 35
BanII G_rGCy'C
Cuts at: 648 1322 1486 2516 648
Size: 674 164 1030 2604
Fragments arranged by size:
2604 1030 674 164
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 233 233
Size: 4472
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3131 4472 2370
Size: 761 1341 2370
Fragments arranged by size:
2370 1341 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BglI GCCn_nnn'nGGC
Cuts at: 545 545
Size: 4472
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4244 1684
Size: 81 335 2144 1912
Fragments arranged by size:
2144 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 769 1400 632
Size: 137 631 3704
Fragments arranged by size:
3704 631 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1648 2527 3019 3317 948
Size: 700 879 492 298 2103
Fragments arranged by size:
2103 879 700 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 838 1825 838
Size: 987 3485
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 1903 2151 2853 4349 1903
Size: 248 702 1496 2026
Fragments arranged by size:
2026 1496 702 248
BsaWI w'CCGG_w
Cuts at: 668 1661 2182 2860 3134 3281 3692 668
Size: 993 521 678 274 147 411 1448
Fragments arranged by size:
1448 993 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 630 660 1373 1403 630
Size: 30 713 30 3699
Fragments arranged by size:
3699 713 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 247
Size: 4472
BseYI C'CCAG_C
Cuts at: 292 1027 1897 2655 3232 3728 292
Size: 735 870 758 577 496 1036
Fragments arranged by size:
1036 870 758 735 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1039
Size: 4472
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 640 720 1486 2264 2454 3246 640
Size: 80 766 778 190 792 1866
Fragments arranged by size:
1866 792 778 766 190 80
BsmI GAATG_Cn'
Cuts at: 834 834
Size: 4472
BsmBI CGTCTCn'nnnn_
Cuts at: 194 194
Size: 4472
Bsp1286I G_dGCh'C
Cuts at: 640 648 720 1322 1486 2097 2190 2264 2454
Size: 8 72 602 164 611 93 74 190
Cuts at: 2454 2516 3246 640
Size: 62 730 1866
Fragments arranged by size:
1866 730 611 602 190 164 93 74
72 62 8
BspEI T'CCGG_A
Cuts at: 668 3692 668
Size: 3024 1448
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1522 1736 2038 2419 1522
Size: 214 302 381 3575
Fragments arranged by size:
3575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4472
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstYI r'GATC_y
Cuts at: 1 397 580 1497 1964 1987 2322 2568 3580
Size: 396 183 917 467 23 335 246 1012
Cuts at: 3580 3666 3678 4024 1
Size: 86 12 346 449
Fragments arranged by size:
1012 917 467 449 396 346 335 246
183 86 23 12
BstZ17I GTA'TAC
Cuts at: 180 180
Size: 4472
Bsu36I CC'TnA_GG
Cuts at: 341 341
Size: 4472
BtgI C'CryG_G
Cuts at: 107 992 107
Size: 885 3587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4128
Fragments arranged by size:
4128 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4472
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 994 2178 3036 994
Size: 1184 858 2430
Fragments arranged by size:
2430 1184 858
EaeI y'GGCC_r
Cuts at: 137 2057 2231 2622 2649 3994 137
Size: 1920 174 391 27 1345 615
Fragments arranged by size:
1920 1345 615 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 192 2495 2705 192
Size: 2303 210 1959
Fragments arranged by size:
2303 1959 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 472 632 769 1400 2297 2729 3476 3744
Size: 171 160 137 631 897 432 747 268
Cuts at: 3744 301
Size: 1029
Fragments arranged by size:
1029 897 747 631 432 268 171 160
137
EcoNI CCTnn'n_nnAGG
Cuts at: 730 730
Size: 4472
EcoO109I rG'GnC_Cy
Cuts at: 435 515 735 1611 435
Size: 80 220 876 3296
Fragments arranged by size:
3296 876 220 80
EcoRI G'AATT_C
Cuts at: 1339 1476 1339
Size: 137 4335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 702 2491 702
Size: 1789 2683
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 1184 1216 2314 2344 2346 2376 220
Size: 32 932 32 1098 30 2 30 2316
Fragments arranged by size:
2316 1098 932 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1043 1511 1043
Size: 468 4004
HpaI GTT'AAC
Cuts at: 1043 1043
Size: 4472
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 500 1492 500
Size: 992 3480
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3143 3327 3143
Size: 184 4288
MscI TGG'CCA
Cuts at: 2233 2233
Size: 4472
MslI CAynn'nnrTG
Cuts at: 1054 2461 2588 1054
Size: 1407 127 2938
Fragments arranged by size:
2938 1407 127
MspA1I CmG'CkG
Cuts at: 142 407 1015 1583 1897 2257 3270 3515 142
Size: 265 608 568 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 608 568 360 314 265 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NcoI C'CATG_G
Cuts at: 107 992 107
Size: 885 3587
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NspI r_CATG'y
Cuts at: 1525 2025 2932 1525
Size: 500 907 3065
Fragments arranged by size:
3065 907 500
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PfoI T'CCnGG_A
Cuts at: 746 1330 746
Size: 584 3888
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PpuMI rG'GwC_Cy
Cuts at: 515 735 515
Size: 220 4252
PsiI TTA'TAA
Cuts at: 695 1162 695
Size: 467 4005
PspXI vC'TCGA_Gb
Cuts at: 358 358
Size: 4472
PstI C_TGCA'G
Cuts at: 1519 2204 1519
Size: 685 3787
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 1015 1897 2257 1015
Size: 882 360 3230
Fragments arranged by size:
3230 882 360
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 1515 2200 3193 3384 33
Size: 1482 685 993 191 1121
Fragments arranged by size:
1482 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 358 927 1627 2542 3034 3296 358
Size: 569 700 915 492 262 1534
Fragments arranged by size:
1534 915 700 569 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
StyI C'CwwG_G
Cuts at: 107 425 431 614 785 992 1606 1960 107
Size: 318 6 183 171 207 614 354 2619
Fragments arranged by size:
2619 614 354 318 207 183 171 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 835 2055 2188 2647 2856 835
Size: 1220 133 459 209 2451
Fragments arranged by size:
2451 1220 459 209 133
TatI w'GTAC_w
Cuts at: 3987 3987
Size: 4472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 630 662 3989 4021 630
Size: 32 3327 32 1081
Fragments arranged by size:
3327 1081 32 32
Tth111I GACn'n_nGTC
Cuts at: 2269 2269
Size: 4472
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 656 840 1967 409
Size: 247 184 1127 2914
Fragments arranged by size:
2914 1127 247 184
XhoI C'TCGA_G
Cuts at: 358 358
Size: 4472
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
XmnI GAAnn'nnTTC
Cuts at: 1339 1339
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsrGI BssHII BstEII BstXI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI