Restriction map for
pF1KB8832
(Circular) (Six-base) MAPSORT of: pF1KB8832.seq Check: 7045 from: 1 to: 4469
pF1KB8832 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 767 767
Size: 4469
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 882 1050 1218 1302 2294 2726 3473 3741 882
Size: 168 168 84 992 432 747 268 1610
Fragments arranged by size:
1610 992 747 432 268 168 168 84
AfeI AGC'GCT
Cuts at: 256 991 256
Size: 735 3734
AflIII A'CryG_T
Cuts at: 662 1821 2925 3902 662
Size: 1159 1104 977 1229
Fragments arranged by size:
1229 1159 1104 977
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AhdI GACnn_n'nnGTC
Cuts at: 688 688
Size: 4469
AleI CACnn'nnGTG
Cuts at: 218 707 832 957 1156 218
Size: 489 125 125 199 3531
Fragments arranged by size:
3531 489 199 125 125
AlwNI CAG_nnn'CTG
Cuts at: 474 1774 3341 474
Size: 1300 1567 1602
Fragments arranged by size:
1602 1567 1300
ApaI G_GGCC'C
Cuts at: 1356 1356
Size: 4469
ApaLI G'TGCA_C
Cuts at: 260 1006 3239 260
Size: 746 2233 1490
Fragments arranged by size:
2233 1490 746
ApoI r'AATT_y
Cuts at: 1473 3822 4078 1473
Size: 2349 256 1864
Fragments arranged by size:
2349 1864 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 771 1048 1216 1489 771
Size: 277 168 273 3751
Fragments arranged by size:
3751 277 273 168
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 461 631 1485 1899 2147 2182 461
Size: 170 854 414 248 35 2748
Fragments arranged by size:
2748 854 414 248 170 35
BanII G_rGCy'C
Cuts at: 213 676 742 1356 1483 2513 213
Size: 463 66 614 127 1030 2169
Fragments arranged by size:
2169 1030 614 463 127 66
BbeI G_GCGC'C
Cuts at: 465 635 1903 2151 465
Size: 170 1268 248 2783
Fragments arranged by size:
2783 1268 248 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 963 997 1395 1429 963
Size: 34 398 34 4003
Fragments arranged by size:
4003 398 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3128 4469 2367
Size: 761 1341 2367
Fragments arranged by size:
2367 1341 761
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglI GCCn_nnn'nGGC
Cuts at: 353 483 941 353
Size: 130 458 3881
Fragments arranged by size:
3881 458 130
BglII A'GATC_T
Cuts at: 1984 4021 1984
Size: 2037 2432
BlpI GC'TnA_GC
Cuts at: 1581 1581
Size: 4469
Bme1580I G_kGCm'C
Cuts at: 264 613 796 1010 1048 1356 1408 2094 2187
Size: 349 183 214 38 308 52 686 93
Cuts at: 2187 3243 264
Size: 1056 1490
Fragments arranged by size:
1490 1056 686 349 308 214 183 93
52 38
BmrI ACTGGGnnnn_n'
Cuts at: 1681 1762 2097 4241 1681
Size: 81 335 2144 1909
Fragments arranged by size:
2144 1909 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 4469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 838
Size: 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3016 3314 1645
Size: 879 492 298 2800
Fragments arranged by size:
2800 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4173 4173
Size: 4469
BsaAI yAC'GTr
Cuts at: 663 1154 1822 663
Size: 491 668 3310
Fragments arranged by size:
3310 668 491
BsaBI GATnn'nnATC
Cuts at: 6 1983 4020 6
Size: 1977 2037 455
Fragments arranged by size:
2037 1977 455
BsaHI Gr'CG_yC
Cuts at: 462 632 698 1900 2148 2850 4346 462
Size: 170 66 1202 248 702 1496 585
Fragments arranged by size:
1496 1202 702 585 248 170 66
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3131 3278 3689 1658
Size: 521 678 274 147 411 2438
Fragments arranged by size:
2438 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1894 2652 3229 3725 1894
Size: 758 577 496 2638
Fragments arranged by size:
2638 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1242 1243 174
Size: 1068 1 3400
Fragments arranged by size:
3400 1068 1
BsiEI CG_ry'CG
Cuts at: 104 228 357 423 1059 1074 2011 2057 3265
Size: 124 129 66 636 15 937 46 1208
Cuts at: 3265 104
Size: 1308
Fragments arranged by size:
1308 1208 937 636 129 124 66 46
15
BsiHKAI G_wGCw'C
Cuts at: 213 264 742 1010 1483 2261 2451 3243 213
Size: 51 478 268 473 778 190 792 1439
Fragments arranged by size:
1439 792 778 478 473 268 190 51
BsiWI C'GTAC_G
Cuts at: 869 869
Size: 4469
BsmI GAATG_Cn'
Cuts at: 250 250
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 213 264 613 676 742 796 1010 1048 1356
Size: 51 349 63 66 54 214 38 308
Cuts at: 1356 1408 1483 2094 2187 2261 2451 2513 3243
Size: 52 75 611 93 74 190 62 730
Cuts at: 3243 213
Size: 1439
Fragments arranged by size:
1439 730 611 349 308 214 190 93
75 74 66 63 62 54 52 51
38
BspEI T'CCGG_A
Cuts at: 3689 3689
Size: 4469
BspHI T'CATG_A
Cuts at: 3645 3645
Size: 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 767 1519 1733 2035 2416 767
Size: 752 214 302 381 2820
Fragments arranged by size:
2820 752 381 302 214
BsrBI CCG'CTC
Cuts at: 50 483 948 1076 1331 2761 2815 4299 4464
Size: 433 465 128 255 1430 54 1484 165
Cuts at: 4464 50
Size: 55
Fragments arranged by size:
1484 1430 465 433 255 165 128 55
54
BsrDI GCAATG_nn'
Cuts at: 2381 2381
Size: 4469
BsrFI r'CCGG_y
Cuts at: 383 422 458 1094 1192 1428 2467 2648 2857
Size: 39 36 636 98 236 1039 181 209
Cuts at: 2857 383
Size: 1995
Fragments arranged by size:
1995 1039 636 236 209 181 98 39
36
BssHII G'CGCG_C
Cuts at: 527 602 652 1362 527
Size: 75 50 710 3634
Fragments arranged by size:
3634 710 75 50
BssSI C'ACGA_G
Cuts at: 150 2740 3098 150
Size: 2590 358 1521
Fragments arranged by size:
2590 1521 358
BstAPI GCAn_nnn'nTGC
Cuts at: 268 329 1846 268
Size: 61 1517 2891
Fragments arranged by size:
2891 1517 61
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 709 709
Size: 4469
BstYI r'GATC_y
Cuts at: 1 1494 1961 1984 2319 2565 3577 3663 3675
Size: 1493 467 23 335 246 1012 86 12
Cuts at: 3675 4021 1
Size: 346 449
Fragments arranged by size:
1493 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 405 2419 2600 2763 405
Size: 2014 181 163 2111
Fragments arranged by size:
2111 2014 181 163
BtsI GCAGTG_nn'
Cuts at: 134 330 613 1255 1406 1712 134
Size: 196 283 642 151 306 2891
Fragments arranged by size:
2891 642 306 283 196 151
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 225 303 354 420 949 1056 1194 1285 2054
Size: 78 51 66 529 107 138 91 769
Cuts at: 2054 2228 2619 2646 3991 225
Size: 174 391 27 1345 703
Fragments arranged by size:
1345 769 703 529 391 174 138 107
91 78 66 51 27
EagI C'GGCC_G
Cuts at: 225 354 420 1056 2054 225
Size: 129 66 636 998 2640
Fragments arranged by size:
2640 998 636 129 66
EarI CTCTTCn'nnn_
Cuts at: 541 2492 2702 541
Size: 1951 210 2308
Fragments arranged by size:
2308 1951 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 334 2677 2987 3133 4279 334
Size: 2343 310 146 1146 524
Fragments arranged by size:
2343 1146 524 310 146
EcoICRI GAG'CTC
Cuts at: 211 740 1481 211
Size: 529 741 3199
Fragments arranged by size:
3199 741 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 882 1050 1218 1302 2294 2726 3473 3741
Size: 44 168 168 84 992 432 747 268
Cuts at: 3741 838
Size: 1566
Fragments arranged by size:
1566 992 747 432 268 168 168 84
44
EcoO109I rG'GnC_Cy
Cuts at: 1608 1608
Size: 4469
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 1299 2250 1299
Size: 951 3518
HaeII r_GCGC'y
Cuts at: 258 429 448 465 635 827 993 1903 2151
Size: 171 19 17 170 192 166 910 248
Cuts at: 2151 3173 4152 258
Size: 1022 979 575
Fragments arranged by size:
1022 979 910 575 248 192 171 170
166 19 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 674 706 2311 2341 2343 2373 674
Size: 32 1605 30 2 30 2770
Fragments arranged by size:
2770 1605 32 30 30 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
HindIII A'AGCT_T
Cuts at: 339 1249 339
Size: 910 3559
KasI G'GCGC_C
Cuts at: 461 631 1899 2147 461
Size: 170 1268 248 2783
Fragments arranged by size:
2783 1268 248 170
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1019 3140 3324 1019
Size: 2121 184 2164
Fragments arranged by size:
2164 2121 184
MscI TGG'CCA
Cuts at: 305 2230 305
Size: 1925 2544
MslI CAynn'nnrTG
Cuts at: 218 661 707 832 957 1156 1209 2458 2585
Size: 443 46 125 125 199 53 1249 127
Cuts at: 2585 218
Size: 2102
Fragments arranged by size:
2102 1249 443 199 127 125 125 53
46
MspA1I CmG'CkG
Cuts at: 136 353 494 501 599 1127 1291 1350 1375
Size: 217 141 7 98 528 164 59 25
Cuts at: 1375 1580 1894 2254 3267 3512 136
Size: 205 314 360 1013 245 1093
Fragments arranged by size:
1093 1013 528 360 314 245 217 205
164 141 98 59 25 7
NaeI GCC'GGC
Cuts at: 424 1194 1430 2650 424
Size: 770 236 1220 2243
Fragments arranged by size:
2243 1220 770 236
NarI GG'CG_CC
Cuts at: 462 632 1900 2148 462
Size: 170 1268 248 2783
Fragments arranged by size:
2783 1268 248 170
NgoMIV G'CCGG_C
Cuts at: 422 1192 1428 2648 422
Size: 770 236 1220 2243
Fragments arranged by size:
2243 1220 770 236
NotI GC'GGCC_GC
Cuts at: 1056 1056
Size: 4469
NspI r_CATG'y
Cuts at: 301 419 878 958 1522 2022 2929 301
Size: 118 459 80 564 500 907 1841
Fragments arranged by size:
1841 907 564 500 459 118 80
PciI A'CATG_T
Cuts at: 2925 2925
Size: 4469
PfoI T'CCnGG_A
Cuts at: 667 815 667
Size: 148 4321
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PmlI CAC'GTG
Cuts at: 663 1154 663
Size: 491 3978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4437
PspOMI G'GGCC_C
Cuts at: 1352 1352
Size: 4469
PstI C_TGCA'G
Cuts at: 325 764 1516 2201 325
Size: 439 752 685 2593
Fragments arranged by size:
2593 752 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 1894 2254 1894
Size: 360 4109
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 213 742 1483 213
Size: 529 741 3199
Fragments arranged by size:
3199 741 529
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 541 2492 2702 541
Size: 1951 210 2308
Fragments arranged by size:
2308 1951 210
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SexAI A'CCwGG_T
Cuts at: 998 998
Size: 4469
SfcI C'TryA_G
Cuts at: 33 180 321 760 1512 2197 3190 3381 33
Size: 147 141 439 752 685 993 191 1121
Fragments arranged by size:
1121 993 752 685 439 191 147 141
SfoI GGC'GCC
Cuts at: 463 633 1901 2149 463
Size: 170 1268 248 2783
Fragments arranged by size:
2783 1268 248 170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SgrAI Cr'CCGG_yG
Cuts at: 458 1094 458
Size: 636 3833
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 1624 2539 3031 3293 1624
Size: 915 492 262 2800
Fragments arranged by size:
2800 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 301 1522 2022 301
Size: 1221 500 2748
Fragments arranged by size:
2748 1221 500
StuI AGG'CCT
Cuts at: 1231 1231
Size: 4469
StyI C'CwwG_G
Cuts at: 560 1603 1957 560
Size: 1043 354 3072
Fragments arranged by size:
3072 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2185 2644 2853 2052
Size: 133 459 209 3668
Fragments arranged by size:
3668 459 209 133
TatI w'GTAC_w
Cuts at: 3984 3984
Size: 4469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 4437
Tth111I GACn'n_nGTC
Cuts at: 2266 2266
Size: 4469
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1964 1964
Size: 4469
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
XmnI GAAnn'nnTTC
Cuts at: 784 1036 784
Size: 252 4217
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PciI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI BsmBI
BsrGI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PasI PflMI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI SwaI XhoI ZraI