Restriction map for
pF1KB8830
(Circular) (Six-base) MAPSORT of: pF1KB8830.seq Check: 4739 from: 1 to: 4433
pF1KB8830 4433 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1024 1449 1024
Size: 425 4008
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 928 1384 2258 2690 3437 3705 431
Size: 497 456 874 432 747 268 1159
Fragments arranged by size:
1159 874 747 497 456 432 268
AflIII A'CryG_T
Cuts at: 1785 2889 3866 1785
Size: 1104 977 2352
Fragments arranged by size:
2352 1104 977
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AlwNI CAG_nnn'CTG
Cuts at: 253 390 1738 3305 253
Size: 137 1348 1567 1381
Fragments arranged by size:
1567 1381 1348 137
ApaLI G'TGCA_C
Cuts at: 3203 3203
Size: 4433
ApoI r'AATT_y
Cuts at: 470 570 806 1437 3786 4042 470
Size: 100 236 631 2349 256 861
Fragments arranged by size:
2349 861 631 256 236 100
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 1453 1453
Size: 4433
BamHI G'GATC_C
Cuts at: 1458 1458
Size: 4433
BanI G'GyrC_C
Cuts at: 1024 1449 1863 2111 2146 1024
Size: 425 414 248 35 3311
Fragments arranged by size:
3311 425 414 248 35
BanII G_rGCy'C
Cuts at: 1187 1447 2477 1187
Size: 260 1030 3143
Fragments arranged by size:
3143 1030 260
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BbsI GAAGACnn'nnnn_
Cuts at: 1061 1152 1061
Size: 91 4342
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 2058 2151 3207 2058
Size: 93 1056 3284
Fragments arranged by size:
3284 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 978 1190 1645 1726 2061 4205 978
Size: 212 455 81 335 2144 1206
Fragments arranged by size:
2144 1206 455 335 212 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2980 3278 1609
Size: 879 492 298 2764
Fragments arranged by size:
2764 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4137 4137
Size: 4433
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 1864 2112 2814 4310 1864
Size: 248 702 1496 1987
Fragments arranged by size:
1987 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1622 2143 2821 3095 3242 3653 1622
Size: 521 678 274 147 411 2402
Fragments arranged by size:
2402 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 159 189 923 953 159
Size: 30 734 30 3639
Fragments arranged by size:
3639 734 30 30
BseYI C'CCAG_C
Cuts at: 1858 2616 3193 3689 1858
Size: 758 577 496 2602
Fragments arranged by size:
2602 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3229 104
Size: 1871 46 1208 1308
Fragments arranged by size:
1871 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1447 2225 2415 3207 1447
Size: 778 190 792 2673
Fragments arranged by size:
2673 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1187 1447 2058 2151 2225 2415 2477 3207 1187
Size: 260 611 93 74 190 62 730 2413
Fragments arranged by size:
2413 730 611 260 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3653 3653
Size: 4433
BspHI T'CATG_A
Cuts at: 3609 3609
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1483 1697 1999 2380 1483
Size: 214 302 381 3536
Fragments arranged by size:
3536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2725 2779 4263 4428 50
Size: 2675 54 1484 165 55
Fragments arranged by size:
2675 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1429 2345 1429
Size: 916 3517
BsrFI r'CCGG_y
Cuts at: 2431 2612 2821 2431
Size: 181 209 4043
Fragments arranged by size:
4043 209 181
BsrGI T'GTAC_A
Cuts at: 1306 1306
Size: 4433
BssSI C'ACGA_G
Cuts at: 2704 3062 2704
Size: 358 4075
BstAPI GCAn_nnn'nTGC
Cuts at: 1810 1810
Size: 4433
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 333 968 333
Size: 635 3798
BstYI r'GATC_y
Cuts at: 1 1458 1925 1948 2283 2529 3541 3627 3639
Size: 1457 467 23 335 246 1012 86 12
Cuts at: 3639 3985 1
Size: 346 449
Fragments arranged by size:
1457 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 401 107
Size: 294 4139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 415 557 1676 415
Size: 142 1119 3172
Fragments arranged by size:
3172 1119 142
ClaI AT'CG_AT
Cuts at: 360 1325 360
Size: 965 3468
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 1088 1156 2139 2997 1088
Size: 68 983 858 2524
Fragments arranged by size:
2524 983 858 68
EaeI y'GGCC_r
Cuts at: 2018 2192 2583 2610 3955 2018
Size: 174 391 27 1345 2496
Fragments arranged by size:
2496 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 450 1103 2456 2666 450
Size: 653 1353 210 2217
Fragments arranged by size:
2217 1353 653 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 2951 3097 4243 2641
Size: 310 146 1146 2831
Fragments arranged by size:
2831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 928 1384 2258 2690 3437 3705 431
Size: 497 456 874 432 747 268 1159
Fragments arranged by size:
1159 874 747 497 456 432 268
EcoO109I rG'GnC_Cy
Cuts at: 730 1203 1572 730
Size: 473 369 3591
Fragments arranged by size:
3591 473 369
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 1214 1867 2115 3137 4116 1214
Size: 653 248 1022 979 1531
Fragments arranged by size:
1531 1022 979 653 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 612 644 2275 2305 2307 2337 612
Size: 32 1631 30 2 30 2708
Fragments arranged by size:
2708 1631 32 30 30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1028 1453 1028
Size: 425 4008
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 145 314 860 3104 3288 145
Size: 169 546 2244 184 1290
Fragments arranged by size:
2244 1290 546 184 169
MscI TGG'CCA
Cuts at: 2194 2194
Size: 4433
MslI CAynn'nnrTG
Cuts at: 350 479 680 2422 2549 350
Size: 129 201 1742 127 2234
Fragments arranged by size:
2234 1742 201 129 127
MspA1I CmG'CkG
Cuts at: 317 1544 1858 2218 3231 3476 317
Size: 1227 314 360 1013 245 1274
Fragments arranged by size:
1274 1227 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 4433
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NcoI C'CATG_G
Cuts at: 107 107
Size: 4433
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 4433
NspI r_CATG'y
Cuts at: 1486 1986 2893 1486
Size: 500 907 3026
Fragments arranged by size:
3026 907 500
PciI A'CATG_T
Cuts at: 2889 2889
Size: 4433
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4401
PpuMI rG'GwC_Cy
Cuts at: 730 1203 730
Size: 473 3960
PsiI TTA'TAA
Cuts at: 1199 1379 1199
Size: 180 4253
PstI C_TGCA'G
Cuts at: 878 1277 1480 2165 878
Size: 399 203 685 3146
Fragments arranged by size:
3146 685 399 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 317 1858 2218 317
Size: 1541 360 2532
Fragments arranged by size:
2532 1541 360
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 1397 3950 1397
Size: 2553 1880
SexAI A'CCwGG_T
Cuts at: 755 755
Size: 4433
SfcI C'TryA_G
Cuts at: 33 874 1086 1273 1476 2161 3154 3345 33
Size: 841 212 187 203 685 993 191 1121
Fragments arranged by size:
1121 993 841 685 212 203 191 187
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 1588 2503 2995 3257 1588
Size: 915 492 262 2764
Fragments arranged by size:
2764 915 492 262
SpeI A'CTAG_T
Cuts at: 67 160 67
Size: 93 4340
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
SspI AAT'ATT
Cuts at: 129 129
Size: 4433
StyI C'CwwG_G
Cuts at: 107 1567 1921 107
Size: 1460 354 2619
Fragments arranged by size:
2619 1460 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2016 2149 2608 2817 2016
Size: 133 459 209 3632
Fragments arranged by size:
3632 459 209 133
TatI w'GTAC_w
Cuts at: 1306 1395 3948 1306
Size: 89 2553 1791
Fragments arranged by size:
2553 1791 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 159 191 3950 3982 159
Size: 32 3759 32 610
Fragments arranged by size:
3759 610 32 32
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 855 1928 855
Size: 1073 3360
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
XmnI GAAnn'nnTTC
Cuts at: 1232 1232
Size: 4433
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI